Enzymes Involved in Naproxen Degradation by Planococcus sp. S5

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 65 , ISSUE 2 (June 2016) > List of articles

Enzymes Involved in Naproxen Degradation by Planococcus sp. S5

Danuta Wojcieszyńska / Dorota Domaradzka / Katarzyna Hupert-Kocurek / Urszula Guzik *

Keywords : Planococcus, biodegradation, naproxen, enzymes induction, aromatic plant compounds

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 2, Pages 177-182, DOI: https://doi.org/10.5604/17331331.1204477

License : (CC BY-NC-ND 4.0)

Received Date : 30-October-2015 / Accepted: 10-January-2016 / Published Online: 07-June-2016

ARTICLE

ABSTRACT

Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. Planococcus sp. S5 is a gram positive strain able to degrade naproxen in monosubstrate culture (27%). However, naproxen is not a suf­ficient growth substrate for this strain. In the presence of benzoate, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid or vanillic acid as growth substrates, the degradation of 21.5%, 71.71%, 14.75% and 8.16% of naproxen was observed respectively. It was shown that the activity of monooxygenase, hydroxyquinol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxyegnase in strain S5 was induced after growth of the strain with naproxen and 4-hydroxybenzoate. Moreover, in the presence of naproxen activity of gentisate 1,2-dioxygenase, enzyme engaged in 4-hydroxybenzoate metabolism, was completely inhibited. The obtained results suggest that monooxygenase and hydroxyquinol 1,2-dioxygenase are the main enzymes in naproxen degradation by Planococcus sp. S5.

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