Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 65 , ISSUE 3 (August 2016) > List of articles

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

Chandran Sathesh-Prabu / Young-Keun Lee *

Keywords : Pleurotus florida, 2D-PAGE, AFLP, genetic variability, mushroom

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 3, Pages 271-277, DOI: https://doi.org/10.5604/17331331.1215606

License : (CC BY-NC-ND 4.0)

Received Date : 23-September-2013 / Accepted: 11-February-2016 / Published Online: 26-August-2016

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ABSTRACT

We report the genetic similarity changes between a mutant mushroom (Pleurotus florida, designated as PfCM4) having increased cellulo­lytic activity developed through radiation mutagenesis and its wild type by amplified fragment length polymorphism (AFLP). On average, 23 AFLP fragments were amplified per primer combination, and a total of 286 polymorphic fragments (78.57% polymorphism) with maxi­mal fragment length of 1365 base pairs (bp) were obtained. The genetic similarity between wild type and PfCM4 was found to be 22.30%. In addition, mycelial and secreted protein profiling by 2D-PAGE showed at least three and five different protein spots in the range of 25 kD to 100 kD, respectively, in PfCM4. It seems that the variation in genetic similarity and different expression of both mycelial and secreted proteins in PfCM4 in comparison to the wild type could likely be correlated with its increased cellulolytic activity effected by the irradiation.

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