Distribution and Identification of Endophytic Streptomyces Species from Schima wallichii as Potential Biocontrol Agents against Fungal Plant Pathogens

Publications

Share / Export Citation / Email / Print / Text size:

Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

GET ALERTS

ISSN: 1733-1331
eISSN: 2544-4646

DESCRIPTION

9
Reader(s)
15
Visit(s)
0
Comment(s)
0
Share(s)

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue / page

Related articles

VOLUME 65 , ISSUE 3 (August 2016) > List of articles

Distribution and Identification of Endophytic Streptomyces Species from Schima wallichii as Potential Biocontrol Agents against Fungal Plant Pathogens

Ajit Kumar Passari / Vineet Kumar Mishra / Vijai Kumar Gupta / Ratul Saikia / Bhim Pratap Singh *

Keywords : Schima wallichii, biocontrol agent, endophytic Streptomyces, nonribosomal peptide synthetase (NRPS), polyketide synthase (PKS-I)

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 3, Pages 319-329, DOI: https://doi.org/10.5604/17331331.1215611

License : (CC BY-NC-ND 4.0)

Received Date : 28-March-2015 / Accepted: 02-February-2016 / Published Online: 26-August-2016

ARTICLE

ABSTRACT

The prospective of endophytic microorganisms allied with medicinal plants is disproportionally large compared to those in other biomes. The use of antagonistic microorganisms to control devastating fungal pathogens is an attractive and eco-friendly substitute for chemi­cal pesticides. Many species of actinomycetes, especially the genus Streptomyces, are well known as biocontrol agents. We investigated the culturable community composition and biological control ability of endophytic Streptomyces sp. associated with an ethanobotanical plant Schima wallichi. A total of 22 actinobacterial strains were isolated from different organs of selected medicinal plants and screened for their biocontrol ability against seven fungal phytopathogens. Seven isolates showed significant inhibition activity against most of the selected pathogens. Their identification based on 16S rRNA gene sequence analysis, strongly indicated that all strains belonged to the genus Streptomyces. An endophytic strain BPSAC70 isolated from root tissues showed highest percentage of inhibition (98.3 %) against Fusarium culmorum with significant activity against other tested fungal pathogens. Phylogenetic analysis based on 16S rRNA gene sequences revealed that all seven strains shared 100 % similarity with the genus Streptomyces. In addition, the isolates were subjected to the amplification of antimicrobial genes encoding polyketide synthase type I (PKS-I) and nonribosomal peptide synthetase (NRPS) and found to be present in most of the potent strains. Our results identified some potential endophytic Streptomyces species having antagonistic activity against multiple fungal phytopathogens that could be used as an effective biocontrol agent against pathogenic fungi.

Content not available PDF Share

FIGURES & TABLES

REFERENCES

Adegboye M.F. and O.O. Babalola. 2012. Taxonomy and ecology of antibiotic producing actinomycetes. Afr. J. Agric. Res. 7: 2255–2261.

 

Anderson A.S. and M.H.E. Wellington. 2001. The taxonomy of Streptomyces and related genera. Int. J. Syst. Evol. Microbiol. 51: 797–814.

 

Anitha A. and M. Rabeeth. 2009. Control of Fusarium wilt of tomato by bioformulation of Streptomyces griseus in green house condition. Afr. J. Basic Appl. Sci. 1: 9–14.

 

Ayuso-Sacido A. and O. Genilloud. 2005. New PCR primers for the screening of NRPS and PKS-I systems in actinomycetes: detection and distribution of these biosynthetic gene sequences in major taxonomic groups. Microb. Ecol. 49: 10–24.

 

Baltz R.H. 2006. Is our antibiotic pipeline unproductive because of starvation, constipation or lack of inspiration? J. Ind. Microbiol. Biotechnol. 33: 507–513.

 

Berdi J. 1989. The discovery of new bioactive metabolites; screening and identification. Prog. Ind. Microbiol. 27: 3–25.

 

Bredholdt H., O.A. Galatenko, K. Engelhardt, E. Fjaervik, L.P. Terekhova and S.B. Zotchev. 2007. Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord, Norway: isolation, diversity and biological activity. Environ. Microbiol. 9: 2756–2764.

 

Brimner T.A. and G.J. Boland. 2003. A review of the non-target effects of fungi used to biologically control plant diseases. Agr. Ecosyst. Environ. 100: 3–16.

 

Buchnan R.E. and N.E. Gibbons. 2000. In: Bergey’s manual of determinative bacteriology. 9th ed. Actinomycetales.

 

Cao L., Z. Qiu, J. You, H. Tan and S. Zhou. 2005. Isolation and Characterization of endophytic Streptomycete antagonists of Fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol. Lett. 247: 147–152.

 

Coombs J.T. and C.M.M. Franco. 2003. Isolation and identification of actinobacteria from surface sterilized wheat roots. Appl. Env. Microbiol. 69: 5603–5608.

 

Coombs J.T., P.P. Michelsen and C.M.M. Franco. 2004. Evaluation of endophytic actinobacteria as antagonists of Gaeumannomyces graminis var. tritici in wheat. Biol. Control. 29: 359–366.

 

Dahiya N., R. Tewari and G.S. Hoondal. 2006. Biotechnolo-gical aspects of chitinolytic enzymes. Appl. Microbiol. Biotechnol. 71: 773–782.

 

De-Araujo M.J., C.A. Silva and J.L. Azevedo. 2000. Isolation of endophytic actinomycetes from roots and leaves of Maize (Zea mays). Braz. Arch. Biol. Techn. 43: 447–451.

 

El-Tarabily K.A., A.H. Nassar, G.E. Hardy and K. Sivasithamparam. 2009. Plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes. J. Appl. Microbiol. 106: 13–26.

 

Evangelista-Martinez Z. 2014. Isolation and characterization of soil Streptomyces species as potential biological control agents against fungal plant Pathogens. World J. Microbiol. Biotechnol. 30: 1639–1647.

 

Ezziyyani M., M.E. Requena, C. Egea-Gilabert and M.E. Candela. 2007. Biological control of Phytophthora root rot of pepper using Trichoderma harzianum and Streptomyces rochei in combination. J. Phytopathol. 155: 342–349.

 

Felsenstein J. 1985. Confidence limits of phylogenies: An approach using the bootstrap. Evol. 39: 783–791.

 

Franco C.M. and L.E. Cautinho. 1991. Detection of novel secondary metabolites. Crit. Rev. Biotechnol. 11: 193–276.

 

Godfrey C.R.A. 1995. Agrochemicals from Natural Products. New York, Marcel Dekker.

 

Gohel V., A. Singh, M. Vimal, P. Ashwini and H.S. Chhatpar. 2006. Bioprospecting and antifungal potential of chitinolytic Microorganisms. Afr. J. Biotechnol. 5: 54–72.

 

Goodfellow M. and J.A. Haynes. 1984. Actinomycetes in marine sediments, pp. 453–472. In: Ortiz-Ortiz L., L.F. Bojalil and V. Yakoleff (eds). Biological, biochemical and biomedical aspects of actinomy-cetes. Academic Press, New York.

 

Graner G., P. Persson, J. Meijer and S. Alstrom. 2003. A study on microbial diversity in different cultivars of Brassica napus in relation to its wilt pathogen, Verticillium longisporum. FEMS Microbiol. Lett. 224: 269–276.

 

Haggag W.M. and A.M. Abdall. 2011. Foliar application of Streptomyces aureofaciens improve protection in Mango against post-harvest anthracnose and enhances fruit yield. Eur. J. Sci. Res. 63: 139–149.

 

Hasegawa S., A. Meguro, M. Shimizu, T. Nishimura and H. Kunoh. 2006. Endophytic actinomycetes and their interactions with host plants. Actinomycetologica 20: 72–81.

 

Hayakawa M., Y. Momose, T. Yamazaki and H. Nonomura. 1996. A method for the selective isolation of Microtetraspora glauca and related fourspored actinomycetes from soil. J. Appl. Bacteriol. 80: 375–386.

 

Hwang B.C., S.J. Ahn and S.S. Moon. 1994. Production, puri-fication and antifungal activity of the antibiotic nucleoside, tubercidin, produced by Streptomyces violaceusniger. Can. J. Bot. 72: 480–485.

 

Intra B., I. Mungsuntisuk, T. Nihira, Y. Igarashi and W. Panbangred. 2011. Identification of actinomycetes from plant rhizospheric soils with inhibitory activity against Colletotrichum sp. the causative agent of anthracnose disease. BMC Res. Notes 4: 98.

 

Kayini A. and R.R. Pandey. 2010. Phyllosphere Fungi of Alnus nepalensis, Castanopsis hystrix and Schima walichii in a Subtropical Forest of North East India. J. Am. Sci. 6: 118–124.

 

Khamna S., A. Yokota and S. Lumyong. 2009a. Actinomycetes isolated from medicinal plant rhizosphere soil: diversity and screening of antifungal compound, indole-3-acetic acid and siderophore production. World J. Microbiol. Biotechnol. 25: 649–655.

 

Khamna S., A. Yokota, J.F. Peberdy and S. Lumyong. 2009b. Antifungal activity of Streptomyces sp. isolated from rhizosphere of Thai medicinal plants. Int. J. Integr. Biol. 6: 143–147.

 

Kim T.U., S.H. Cho, J.H. Han, Y.M. Shin, H.B. Lee and S.B. Kim. 2012. Diversity and physiological properties of root endophytic actinobacteria in native herbaceous plants of Korea. J. Microbiol. 50: 50–57.

 

Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111–120.

 

Kizuka M., R. Enokita, K. Takahashi, Y. Okamoto, T. Otsuka, Y. Shigematsu, Y. Inoue and T. Okazaki. 2002. Studies on actinomycetes isolated from plant leaves: New plant growth inhibitors A-79197-2 and -3 from Dacthylosporangium aurantiacum SANK 61299. Actinomycetologica 16: 14–16.

 

Kumar V., A. Bharti, O. Gusain and G.S. Bisht. 2011. Scanning electron microscopy of Streptomyces without use of any chemical fixatives. Scanning 33: 1–4.

 

Kuster E. and S. Williams. 1964. Selection of media for isolation of Streptomycetes. Nat. 202: 928–929.

 

Maldonado M.C., C.E. Orosco, M.A. Gordillo and A.R. Navarro. 2010. In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum. Afr. J. Microbiol. Res. 4: 2451–2556.

 

Mingma R., W. Pathom-aree, S. Trakulnaleamsai, A. Thamchaipenet and K. Duangmal. 2014. Isolation of rhizospheric and roots endophytic actinomycetes from Leguminosae plant and their activities to inhibit soybean pathogen, Xanthomonas campestris pv. Glycine. World J. Microbiol. Biotechnol. 30: 271–280.

 

Nimnoi P., N. Pongsilp and S. Lumyong. 2010. Endophytic actinomycetes isolated from Aquilaria crassna Pierre ex Lec and screening of plant growth promoters production. World J. Microbiol. Biotechnol. 26: 193–203.

 

Oskay M. 2009. Antifungal and antibacterial compounds from Streptomyces strains. Afr. J. Biotechnol. 8: 3007–3017.

 

Passari A.K., Mishra V.K., Saikia R., Gupta V.K. and Singh B.P. 2015. Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential. Front. Microbiol. 6: 273.

 

Patil N.N., M.S. Waghmode, P.S. Gaikwad, M.H. Gajbhiye, A.B. Gunjal, N.N. Nawani and B.P. Kapadnis. 2014. Potential of Microbispora sp. V2 as biocontrol agent against Sclerotium rolfsii the causative agent of southern blight of Zea mays L (Baby corn) in vitro studies. Indian J. Exp. Biol. 52: 1147–1151.

 

Qin S., J. Li, H.H. Chen, G.Z. Zhao and W.Y. Zhu. 2009. Isolation, diversity and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl. Environ. Microbiol. 75: 6176–6186.

 

Qin Z., V. Peng, X. Zhou, R. Liang, Q. Zhou, H. Chen, D.A. Hopwood, T. Keiser and Z. Deng. 1994. Development of a gene cloning system for Streptomyces hygroscopicus var yingchengensis, a pro-ducer of three useful antifungal compounds by elimination of three barriers to DNA transfer. J. Bacteriol. 176: 2090–2095.

 

Raatikainen O.J., T.H. Paivinen and R.T. Tahvonen. 1994. HPLC separation and subsequent detection of aromatic heptaene polyenes in peat after treatment with Streptomyces griseoviridis. Pestic. Sci. 41: 149–154.

 

Rothrock C.S. and D. Gottlieb. 1984. Role of antibiosis in antagonism of Streptomyces hygroscopicus var geldanus to Rhizoctonia solani in soil. Can. J. Microbiol. 30: 1440–1447.

 

Sardi P., M. Saracchi, S. Quaroni, B. Petrolini, G.E. Borgo-novi and S. Merli. 1992. Isolation of endophytic Streptomyces strains from surface-sterilized roots. Appl. Environ. Microbiol. 58: 2691–2693.

 

Shimizu M., T. Furumai, Y. Igarashi, H. Onaka, T. Nishimura, R. Yoshida and H. Kunoh. 2001. Association of induced disease resistance of rhododendron seedlings with inoculation of Strepto-myces sp. R-5 and treatment with actinomycin D and amphotericin B to the tissue-culture medium. J. Anti. Biot. 54: 501–505.

 

Shimizu M., A. Meguro, S. Hasegawa, T. Nishimura and H. Kunoh H. 2006. Disease resistance induced by nonantagonistic endophytic Streptomyces sp. on tissue-cultured seedlings of rhododendron. J. Gen. Plant Pathol. 72: 351–354.

 

Shimizu M., S. Yazawa, Y. Ushijima. 2009. A promising strain of endophytic Streptomyces sp. for biological control of cucumber anthracnose. J. Gen. Plant Pathol. 75: 27–36.

 

Simone D.G.P., M.M. Rosana, R.R.C. Rosalie, N.L.M. Maria, H.B. Marta and B.V. Alane. 1999. Influence of growth medium in protease and pigment production in Streptomyces cyzneus. Mem. Inst. Oswwaldo. Cruz. 94: 173–177.

 

Smith J., A. Putnam and M. Nair. 1990. In vitro control of Fusarium diseases of Asparagus officinalis L. with a Streptomyces or its polyene antibiotic, Faeriefungin. J. Agric. Food Chem. 38: 1729–1733.

 

Stone J.K., C.W. Bacon and J.F. White. 2000. An overview of endophy-tic microbes: endophytism defined, pp. 3–29. In: Bacon C.W. and J.F. White (eds). Microbial endophytes. Marcel Dekker, Inc., New York.

 

Strobel G.A. and B. Daisy. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiol. Mol. Biol. Rev. 67: 491–502.

 

Taechowisan T., C. Lu, Y. Shen and S. Lumyong. 2005. Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activity. Microbiology 151: 1691–1695.

 

Taechowisan T. and S. Lumyong. 2003. Activity of endophytic actinomycetes from roots of Zingiber officinale and Alpinia galanga against phytopathogenic fungi. Ann. Microbiol. 53: 291–298.

 

Taechowisan T., J.F. Peberdy and S. Lumyong. 2003. Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J. Microbiol. Biotechnol. 19: 381–385.

 

Thompson J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analys is tools. Nucleic Acids Res. 24: 4876–4882.

 

Trejo-Estrada S.L., A. Paszczynski and D.L. Crawford. 1998. Antibiotics and enzymes produced by biological agent Strepto-myces violaceusniger YCED-9. J. Ind. Microbiol. Biotechnol. 21: 81–90.

 

Verma V.C., S.K. Gond, A. Kumar, A. Mishra, R.N. Kharwar and A.C. Gange. 2009. Endophytic actinomycetes from Azadirachta indica A. Juss.: isolation, diversity, and anti-microbial activity. Microb. Ecol. 57: 749–756.

 

Zhao S., C.M. Du and C.Y. Tian. 2012. Suppression of Fusarium oxysporum and induced resistance of plants involved in the biocontrol of cucumber Fusarium wilt by Streptomyces bikiniensis HD-087. World J. Microbiol. Biotechnol. 28: 2919–2927.

 

EXTRA FILES

COMMENTS