SEARCH WITHIN CONTENT
Citation Information : Polish Journal of Microbiology. Volume 65, Issue 3, Pages 353-357, DOI: https://doi.org/10.5604/17331331.1215614
License : (CC BY-NC-ND 4.0)
Received Date : 22-January-2016 / Accepted: 06-June-2016 / Published Online: 26-August-2016
Microbial colonisation of chronic venous ulcers and synergism between bacterial species slow down the healing process. The study aimed at performing qualitative analysis of microbial flora in venous leg ulcers treated with platelet rich plasma (PRP). Twenty two women and twelve men aged 47–90 years were treated with PRP at our department between 2012 and 2015. Ulcer cultures collected before and after PRP therapy yielded 83 and 110 microbial isolates, respectively, of Gram positive, Gram negative bacteria and candida. Pseudomonas aueruginosa and Staphylococcus aureus were the most common pre-and post-treatment isolates. PRP therapy and increased the variety of microbial flora.
Alinovi A., P. Bassissi and M. Pini. 1986. Systemic administration of antibiotics in the management of venous ulcers. A randomized clinical trial. J. Am. Acad. Dermatol. 15: 186–191.
Anitua E., I. Andia, B. Ardanza, P. Nurden and A.T. Nurden. 2004. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb. Haemost. 91: 4–15.
Bielecki T.M., T.S. Gazdzik, J. Arendt, T. Szczepański, W. Król and T. Wielkoszyński. 2007. Antibacterial effect of autologous platelet gel enriched with growth factors and other active substances: an in vitro study. J. Bone Joint Surg. Br. 89: 417–420.
Bowler P.G. 2002. Wound pathophysiology, infection and therapeutic options. Ann. Med. 34: 419–427.
Carter M.J., C.P. Fylling and L.K. Parnell. 2011. Use of platelet rich plasma gel on wound healing: a systematic review and meta-analysis. Eplasty. 11: e38.
Cieślik-Bielecka A., D.M. Dohan Ehrenfest, A. Lubkowska and T. Bielecki. 2012. Microbicidal properties of leukocyte-and platelet-rich plasma/fibrin (L-PRP/L-PRF): new perspectives. J. Biol. Regu. Homeost. Agents. 26: 43–52
Eppley B.L., J.E. Woodell and J. Higgins. 2004. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast. Reconstr. Surg. 114: 1502–1508.
Everts P.A., C. Brown Mahoney, J.J. Hoffmann, J.P. Schönberger, H.A. Box, A.van Zundert and J.T. Knape. 2006. Platelet-rich plasma preparation using three devices: implications for platelet activation and platelet growth factor release. Growth Factors. 24: 165–171.
Gjødsbøl K, J.J. Christensen, T. Karlsmark, B. Jørgensen, B.M. Klein and K.A. Krogfelt. 2006. Multiple bacterial species reside in chronic wounds: a longitudinal study. Int Wound J. 3: 225–231.
Howard D.P., A. Howard, A. Kothari, L. Wales , M. Guest and A.H. Davies. 2008. The role of superficial venous surgery in the management of venous ulcers: a systematic review.
Li G.Y., J.M. Yin, H. Ding, W.T. Jia and C.Q. Zhang. 2013. Efficacy of leukocyte-and platelet-rich plasma gel (L-PRP gel) in treating osteomyelitis in a rabbit model. J. Orthop. Res. 31: 949–956.
Lim T., B. Mwipatayi, R. Murray, K. Sieunarine, M. Abbas and D. Angel. 2006. Microbiological profile of chronic ulcers of the lower limb: a prospective observational cohort study. ANZ J. Surg. 76: 688–692.
Lubkowska A., Dołęgowska B. and G. Banfi. 2012. Growth factor content in PRP and their applicability in medicine. J. Biol. Regul. Homeost. Agents. 26: 3–22.
Martinez-Zapata M.J., A.J. Martí-Carvajal, I. Solà, J.A. Expósito, I. Bolíbar, L. Rodríguez and J. Garcia. 2012. Autologous platelet-rich plasma for treating chronic wounds. http://onlinelibrary.wiley. com/doi/10.1002/14651858.CD006899.pub2/full, 2012.10.17.
Moojen D.J., P.A. Everts, R.M. Schure, E.P. Overdevest, A. van Zundert, J.T. Knape, R.M. Castelein, L.B. Creemers and W.J. Dhert. 2008. Antimicrobial activity of platelet-leukocyte gel against Staphylococcus aureus. J. Orthop. Res. 26: 404–410.
Morimoto N., S. Takemoto, K. Kawai, R. Aya, T. Ishisaka and S. Suzuki. 2014. Immediate evaluation of neovascularization in a grafted bilayered artificial dermis using laser Doppler imaging. Ann. Plast. Surg. 72: 84–88.
Rosales A., G. Sandbaek and J.J. Jørgensen. 2010. Stenting for chronic post-thrombotic vena cava and iliofemoral venous occlusions: mid-term patency and clinical outcome. Eur. J. Vasc. Endovasc. Surg. 36: 458–465.
Ross R., J. Glomset, B. Kariyja and L. Harker. 1974. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc. Natl. Acad. Sci. USA 71: 1207–1210.
Saad Setta H., A. Elshahat, K. Elsherbiny, K. Massoud and I. Safe. 2011. Platelet-rich plasma versus platelet-poor plasma in the management of chronic diabetic foot ulcers: a comparative study. Int. Wound. J. 8: 307–312.
Scimeca C.L., M. Bharara, T.K. Fisher, H. Kimbriel and D.G. Armstrong. 2010. Novel use of platelet-rich plasma to augment curative diabetic foot surgery. J. Diabetes Sci. Technol. 4: 1121–1126.