Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic Ganoderma boninense

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 65 , ISSUE 3 (August 2016) > List of articles

Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic Ganoderma boninense

Nisha Thopla Govender * / Maziah Mahmood / Idris Abu Seman / Wong Mui-Yun *

Keywords : basidiocarp, basidiospore, incubation, lytic enzyme

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 3, Pages 383-388, DOI: https://doi.org/10.5604/17331331.1215619

License : (CC BY-NC-ND 4.0)

Received Date : 14-November-2015 / Accepted: 02-February-2016 / Published Online: 26-August-2016

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ABSTRACT

Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological poten­tials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 104 basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 × 106 single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period).

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REFERENCES

Adams D.J. 2004. Fungal cell wall chitinases and glucanases. Microbiology 150: 2029–2035.

 

An Z. 2005. Handbook of Industrial Mycology. CRC Press: Marcel Dekker New York.

 

Annamalai P. and D. Lalithakumari. 1991. Isolation and regeneration of protoplasts from mycelium of Dreschlera oryzae. J. Plant Dis. Prot. 98: 197–204.

 

Choi S.H., B.K. Kim, H.W. Kim, J.H. Kwak, E.C. Choi, Y.C. Kim,Y.B. Yoo and Y.H. Park. 1987. Studies on protoplast formation and regeneration of Ganoderma lucidum. Arch. Pharm. Res. 10(3): 158–164.

 

Kamada T. 2002. Molecular genetics of sexual development in the mushroom Coprinus cinereus. Bioessay 24: 449–459.

 

Kelly F.D. and Nurse P. 2011. de Novo growth zone formation from fission yeast spheroplasts. PLoS One 6: e27977.

 

Lalithakumari D. 1996. Protoplasts-A biotechnological tool for plant pathological studies. Ind. Phytopath. 49: 199–212.

 

Liu T.H., M.J. Lin and W.H. Ko. 2010. Factors affecting protoplast formation by Rhizoctonia solani. New Biotechnology, DOI:10.1016/j.nbt.2009.08.009.

 

Mendoza C.G. 1992. Cell wall structure and protoplast reversion in basidiomycete. World J. Microbiol. Biotechnol. 8(Suppl. 1).

 

Michielse C.B., M. Arentshorst, A.F.J. Ram and C.A.M.J.J. van den Hondel. 2005. Agrobacterium- mediated transformation leads to improved gene replacement efficiency in Aspergillus awamori. Fungal Genet. Biol. 42: 9–19.

 

Rivera A.L., D. Magana-Ortiz and M. Gomez-Lim. 2014. Physical methods for genetic transformation of fungi and yeast. Phs Life Rev. 11: 184–203.

 

Robinson H.L. and J.W. Deacon. 2001. Protoplast preparation and transient transformation of Rhizoctani solani. Mycol. Res.105: 1295–1303.

 

Souza A.G.C., S. Herrero, L.A. Mafia and M.E. Daub. 2014.Methods for Cercospora coffeicola protoplast isolation and genetic transformation with green fluorescent protein. Euro. J. Plant Pathol. 139: 241–244.

 

Shi L., X. Fang, M. Li, D. Mu, A. Ren and M. Zhao. 2012. Development of simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum. World J. Microbiol. Biotechnol. 28: 283–291.

 

Wang M., S. Liu, Y. Li, R. Xu, C. Lu and Y. Shen. 2010. Protoplast mutation and genome shuffling induce the endophytic fungus tubercularia sp. TF5 to produce new compounds. Curr. Microbiol. 61: 254–260.

 

Yan P.S., J.H. Jiang and W.S. Cui. 2004. Characterization of protoplast prepared from the edible fungus, Stropharia rugosa-annulata. World J. Microbiol. Biotechnol. 20: 173–177.

 

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