Expiration Date Prediction of Biocontrol Agent Prepared with Bacillus subtilis B579 Using the Accelerated Aging Method


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 65 , ISSUE 4 (December 2016) > List of articles

Expiration Date Prediction of Biocontrol Agent Prepared with Bacillus subtilis B579 Using the Accelerated Aging Method

Yu Zheng * / Xiaoyang Jiao / Fang Chen / Xuelian Wang / Min Wang *

Keywords : Bacillus subtilis, accelerated aging, biocontrol agent, expiration date

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 4, Pages 461-464, DOI: https://doi.org/10.5604/17331331.1227672

License : (CC BY-NC-ND 4.0)

Received Date : 19-December-2014 / Accepted: 06-June-2016 / Published Online: 28-December-2016



The expiration date of biopesticidal products is an essential feature of their use and storage. In the present work, the expiration date of biocontrol agent was predicted using the accelerated aging method. The available bacteria in Bacillus subtilis B579 biocontrol agent were 3.7 ± 0.2 × 1011 CFU/g. It is calculated that the expiration date of the agent was about 17 months at 25°C. During this period, the available bacteria retained more than 90% of the value in the initial product. Thus, this work suggests the expiration date of biocontrol agents com­posed with spores could be estimated using the accelerated aging method.

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