Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 65 , ISSUE 4 (December 2016) > List of articles

Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods

Agnieszka Figas * / Magdalena Wieczorek / Bogumiła Litwińska / Włodzimierz Gut

Keywords : cell lines L20B and RD, environmental surveillance, poliovirus, sewage

Citation Information : Polish Journal of Microbiology. Volume 65, Issue 4, Pages 479-483, DOI:

License : (CC BY-NC-ND 4.0)

Received Date : 14-December-2015 / Accepted: 08-March-2016 / Published Online: 28-December-2016



The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.

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Al-Khayat Z. and A. M. Ahmad. 2012. Antiviral activity of enviroxime against Polio virus and Rubella virus in tissue culture. Ibnosina J. Med. BS. 4: 9–12.


AW T.G. and K.Y. Gin. 2010. Environmental surveillance and molecular characterization of human enteric viruses in tropical urban wastewaters. J. Apply. Microbiol. 109: 716–730.


Bodian D. and D.M. Horstmann. 1965. Polioviruses, pp. 430–473. In: Horstfal F.L. and I. Tamm. Viral and Rickettsial Infections of Man, 4th ed. Lippincott J.B., Philadelphia.


Dedepsidis E., Z. Kyriakopoulou, V. Pliaka, C. Kottaridi, E. Bolanaki, S. Le vidiotou-Stefanou, D. Komiotis and P. Markoulatos. 2007. Retrospective characterization of a vaccine-derived poliovirus type 1 isolate from sewage in Greece. Appl. Environ. Microbiol. 73: 6697–6704.


Feachem R.G., D.J. Bradley, H. Garelick and D.D. Mara. 1983. Sanitation and disease: health aspects of excreta and wastewater management. John Wiley & Sons, Chichester, U.K.


Hovi T., L.M. Shulman, H. van der Avoort, J. Deshpande, M. Roivainen and E.M. de Gourville. 2010. Role of environmental poliovirus surveillance in global polio eradication and beyond. Epidemiol. Infect. 140: 1–13.


Katayama H., A. Shimasaki and S. Ohgaki. 2002. Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater. Appl. Environ. Microbiol. 68: 1033–1039.


Kern A., M. Kadar, K. Szomor, G. Berencsi, B. Kapusinszky and M. Vargha. 2013. Detection of enteric viruses in Hungarian surface waters: first steps towards environmental surveillance. J. Water Health. 11: 772–782.


Kew O.M., P.F. Wright, V.I. Agol, F. Delpeyroux, H. Shimizu, N. Nathanson and M.A. Pallansch. 2004. Circulating vaccine-derived polioviruses: current state of knowledge. Bull WHO. 82: 16–23.


Kew O.M., R.W. Sutter, E.M. de Gourville, W.R. Dowdle and M.A. Pallansch. 2005. Vaccine-derived polioviruses and the endgame strategy for global polio eradication. Annu. Rev. Microbiol. 59: 587–635.


Kocwa-Haluch R. and B. Zalewska. 2002. Presence of Rotavirus hominis in sewage and water. Polish. J. Environ. 11: 751–755.


Landsteiner K and E. Popper. 1909. Transmission of poliomyelitis on mokeys (in German). Z. Immunitatstorsch. Orig. 2: 377–390.


Li D., A. Gu, Y. Wan, M. He, X. Hu and H.C. Shi. 2010. An integrated cell culture and reverse transcription quantitative PCR assay for detection of infectious rotaviruses in environmental waters. J. Microbiol. Methods. 82: 59–63.


Melnick J.L. 1996. Enteroviruses: polioviruses, coxsackieviruses, echoviruses and newer enteroviruses, pp. 655–712. In: Virology, Fields B.N. Raven Press, New York.


Parasidis T.A., I.G. Alexandropoulou, T.G. Konstantinidis, M. Panopoulou and T.C. Constantinidis. 2013. Epidemiological surveillance of enteric viruses in sewage samples in East Macedonia and Thrace region in Greece. J. Apply. Virol. 2: 9–18.


Pavlov D., W.B. van Zyl, J. van Heerden, W.O.K. Grabow and M.M. Ehlers. 2005. Prevalence of vaccine-derived polioviruses in sewage and river water in South Africa. Water Res. 39: 3309–3319.


Sabin A.B. 1956. Pathogenesis of poliomyelitis. Reappraisal in the light of new data. Science 123: 1151–1157.


World Health Organization (WHO). 2003. Guidenlines for environmental surveillance of poliovirus circulation.


World Health Organization (WHO). 2004. Polio laboratory manual, 4th edition.


World Health Organization (WHO). 2007.World Health Organization Regional Office for Europe. Wild poliovirus isolated in Switzerland’s sewer system, insignificant risk of outbreak. Monthly AFP surveillance bulletin., data dostepu?


Wieczorek M., A. Ciąćka, A. Witek, Ł. Kuryk, A. Żuk-Wasek. 2015. Environmental surveillance of non-polio enteroviruses in Poland, 2011. Food Environ. Virol. 7: 224–231.


Witek A. and M. Wieczorek. 2009. Virological and molecularmethods in enteroviruses infections in 2004–2008, Poland (inPolish). Przegl. Epidemiol. 63: 379–382.


Wood D.J. and B. Hull. 1999. L20B cells simplify culture of polioviruses from clinical samples. J. Med. Virol. 58: 188–192.


Vinje J., N. Gregoricus, J. Martin, H.E. Gary, V.M. Cáceres,L. Venczel, A. Macadam, J.A. Dobbins, C. Burns, D. Wait and others. 2004. Isolation and characterization of circulating type 1 vaccine-derived poliovirus from sewage and stream waters in Hispaniola. J. Infect. Dis. 189: 1168–1175.


Zurbriggen S., K. Tobler, C. Abril, S. Diedrich, M. Ackermann, M.A. Pallansch and A. Metzler. 2008. Isolation of sabin-like polioviruses from wastewater in a country using inactivated polio vaccine. Appl. Environ. Microbiol. 74: 5608–5614.