Deletion of atoR from Streptococcus pyogenes Results in Hypervirulence in a Mouse Model of Sepsis and is LuxS Independent

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 1 (March 2017) > List of articles

Deletion of atoR from Streptococcus pyogenes Results in Hypervirulence in a Mouse Model of Sepsis and is LuxS Independent

Izabela Sitkiewicz * / James M. Musser

Keywords : Streptococcus pyogenes, ato, host-pathogen interactions, short chain fatty acid synthesis, virulence factors

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 1 , ISSN (Online) 2544-4646, DOI: 10.5604/17331331.1234989, March 2017

License : (CC BY-NC-ND 4.0)

Received Date : 17-November-2016 / Accepted: 27-January-2017 / Published Online: 30-March-2017

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ABSTRACT

Group A Streptococcus (GAS) is a Gram-positive human pathogen that causes a variety of diseases ranging from pharyngitis to life-threaten­ing streptococcal toxic shock syndrome. Recently, several global gene expression analyses have yielded extensive new information regarding the regulation of genes encoding known and putative virulence factors in GAS. A microarray analysis found that transcription of the GAS gene M5005_Spy_1343 was significantly increased in response to interaction with human polymorphonuclear leukocytes. M5005_Spy_1343 is predicted to encode a member of the LysR family of transcriptional regulators and is located upstream of a putative operon containing six genes. Five of these genes have sequence similarity to genes involved in short-chain fatty acid metabolism, whereas the sixth gene (luxS) is found in many bacterial species and is involved in quorum sensing. Unexpectedly, inactivation of the M5005_Spy_1343 gene resultedin hypervirulence in an intraperitoneal mouse model of infection. Increased virulence was not due to changes in luxS gene expression.We postulate that short-chain fatty acid metabolism is involved in GAS pathogenesis.

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