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Citation Information : Polish Journal of Microbiology. Volume 66, Issue 1, Pages 57-65, DOI: https://doi.org/10.5604/17331331.1234993
License : (CC BY-NC-ND 4.0)
Received Date : 24-December-2015 / Accepted: 12-April-2016 / Published Online: 30-March-2017
Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.
Adesemoye A.O., H.A. Torbert and J.W. Kloepper. 2009. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecol. 58: 921–929.
Afshan M., M. Kaleem, A. Sohail, H. Asma and I. Nasir. 2015. Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Front. Microbiol. 1: 6.
Alam M.M. and J.K. Ladha. 2004. Optimizing phosphorus fertilization in an intensive vegetable-rice cropping system. Biol. Fert. Soils. 40: 277–283.
Chopade B.A., D.P. Sachdev, H.G. Chaudhari, V.M. Kasture and D.D. Dhavale. 2009. Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth. In. J. Exp. Biol. 47: 993–1000.
Chung H., M. Park, M. Madhaiyan, S. Seshadri, J. Song, H. Cho and T. Sa. 2005. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea. Soil Biol. Biochem. 37: 1970–1974.
Ding Y., J. Wang, Y. Liu and S. Chen. 2005. Isolation and identification of nitrogen-fixing bacilli from plant rhizospheres in Beijing region. J. Appl. Microbiol. 99: 1271–1281.
Fernández L., P. Zalba, M. Gómez and M. Sagardoy. 2007. Phosphate-solubilization activity of bacterial strains in soil and their effect on soybean growth under greenhouse conditions. Biol. Fert. Soils. 43: 805–809.
Gordon S.A. and R.P. Weber. 1951. Colorimetric estimation of indoleacetic acid. Plant Physiol. 26: 192.
Grönemeyer J., C. Burbano, T. Hurek and B. Reinhold-Hurek. 2011. Isolation and characterization of root-associated bacteria from agricultural crops in the Kavango region of Namibia. Plant and Soil. 356: 67–82.
Guiñazú L.B., J.A. Andrés, M.F. Del Papa, M. Pistorio and S.B. Rosas. 2010. Response of alfalfa (Medicago sativa L.) to single and mixed inoculation with phosphate-solubilizing bacteria and Sinorhizobium meliloti. Biol. Fert. Soils. 46:185–190.
Gulati A., N. Sharma, P. Vyas, S. Sood, P. Rahi, V. Pathania and R. Prasad. 2010. Organic acid production and plant growth promotion as a function of phosphate solubilization by Acinetobacter rhizosphaerae strain BIHB 723 isolated from the cold deserts of the trans-Himalayas. Arch. Microbiol. 192: 975–983.
Gyaneshwar P., G. Naresh Kumar, L.J. Parekh and P.S. Poole. 2002. Role of soil microorganisms in improving P nutrition of plants. Plant and Soil 245: 83–93.
Hafeez F.Y., S. Yasmin, D. Ariani, U.R. Mehboob, R.Y. Zafar and K.A. Malik. 2006. Plant growth-promoting bacteria as biofertilizer. Agron. Sust. Develop. 26: 143–150.
Hoagland D.R. and D.I. Arnon. 1950. The water-culture method for growing plants without soil. Circ. Calif. Agric. Exp. Stn. 347: 4–31.
Holt J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley and S.T. Williams. 1994. Bergey’s manual of determinative bacteriology. Williams and Wilkins, Baltimore, USA.
Johri B. 2011. Bacterial diversity in a bagasse-based compost prepared for the cultivation of edible mushrooms Agaricus bisporus. J. Agr. Technol. 7: 1303–1311.
Johri J.K., S. Surange and C.S. Nautiyal. 1999. Occurrence of salt, pH, and temperature-tolerant, phosphate-solubilizing bacteria in alkaline soils. Curr. Microbiol. 39: 89–93.
Kumar R.S., N. Ayyadurai, P. Pandiaraja, A.V. Reddy, Y. Venkateswarlu, O. Prakash and N. Sakthivel. 2005. Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits. J. Appl. Microbiol. 98: 145–154.
Laguerre G., M.R. Allard, F. Revoy and N. Amarger. 1994. Rapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Appl. Environ. Microbiol. 60: 56–63.
Li H.J., H.Y. Chen, L.L. Fan, Z.H. Jiao, Q.H. Chen and Y.C. Jiao. 2015. In vitro antioxidant activities and in vivo anti-hypoxic activity of the edible mushroom Agaricus bisporus (lange) sing. chaidam. Molecules. 20: 17775.
Liu X., L. Wang, C. Zhang, H. Wang, X. Zhang and Y. Li. 2015. Structure characterization and antitumor activity of a polysaccharide from the alkaline extract of king oyster mushroom. Carbohy. Poly. 118: 101–106.
Nautiyal C.S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol. Lett. 170: 265–270.
Park J.H., N. Bolan, M. Megharaj and R. Naidu. 2011, Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. J. Hazard. Mater. 185: 829–836.
Park M., C. Kim, J. Yang, H. Lee, W. Shin, S. Kim and T. Sa. 2005. Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea. Microbiol. Res. 160: 127–133.
Poonguzhali S., M. Madhaiyan and T. Sa. 2008. Isolation and identification of phosphate solubilizing bacteria from chinese cabbage and their effect on growth and phosphorus utilization of plants. J. Microbiol. Biotechnol. 18: 773–777.
Puente M., C. Li and Y. Bashan. 2004. Microbial populations and activities in the rhizoplane of rock-weathering desert plants. II. Growth promotion of cactus seedlings. Plant Biol. 6: 643–650.
Rodríguez H. and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17: 319–339.
Sæbø A. and F. Ferrini. 2006. The use of compost in urban green areas-A review for practical application. Urban. Urban. Greening. 4: 159–169.
Sambrook J., E.F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Press, New York.
Schwyn B. and J.B. Neilands. 1987. Universal chemical assay for the detection and determination of siderophores. Analy. Biochem. 160: 47–56.
Tamura K., J. Dudley, M. Nei and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596–1599.
Teather R.M. and P.J. Wood. 1982. Use of congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43: 777–780.
Ullah S. and A. Bano. 2015. Isolation of plant-growth-promoting rhizobacteria from rhizospheric soil of halophytes and their impact on maize (Zea mays L.) under induced soil salinity. Can. J. Microbiol. 61: 307–313.
Vassilev N. and M. Vassileva. 2003. Biotechnological solubilization of rock phosphate on media containing agroindustrial wastes. Appl. Environ. Microbiol. 61: 435–440.
Vessey J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255: 571–586.
Watanabe F. and S. Olsen. 1965. Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Sci. Soci. Ame. J. 29: 677–678.
Yegorenkova I.V., S.A. Konnova, V.N. Sachuk and V.V. Ignatov. 2001. Azospirillum brasilense colonisation of wheat roots and the role of lectin-carbohydrate interactions in bacterial adsorption and root-hair deformation. Plant and Soil 231: 275–282.
Yu X., X. Liu, T. Zhu, G. Liu and C. Mao. 2011. Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization. Biol. Fert. Soils. 47: 437–446.