Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 66 , ISSUE 1 (March 2017) > List of articles

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Lisa Lombardi / Marina Zoppo / Cosmeri Rizzato / Colin Gerard Egan / Roberto Scarpato / Arianna Tavanti *

Keywords : Pseudomonas stutzeri, AFLP, polymorphisms, UVA exposure

Citation Information : Polish Journal of Microbiology. Volume 66, Issue 1, Pages 107-111, DOI:

License : (CC BY-NC-ND 4.0)

Received Date : 23-December-2015 / Accepted: 10-May-2016 / Published Online: 30-March-2017



Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains compared to unexposed controls. AFLP showed a high discriminatory power, confirming the existence of different genotypes within the species and presence of DNA polymorphismsin UVA-exposed colonies.

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