Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Publications

Share / Export Citation / Email / Print / Text size:

Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

GET ALERTS

ISSN: 1733-1331
eISSN: 2544-4646

DESCRIPTION

7
Reader(s)
15
Visit(s)
0
Comment(s)
0
Share(s)

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue / page

Related articles

VOLUME 66 , ISSUE 1 (March 2017) > List of articles

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Lisa Lombardi / Marina Zoppo / Cosmeri Rizzato / Colin Gerard Egan / Roberto Scarpato / Arianna Tavanti *

Keywords : Pseudomonas stutzeri, AFLP, polymorphisms, UVA exposure

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 1 , ISSN (Online) 2544-4646, DOI: 10.5604/17331331.1234998, March 2017

License : (CC BY-NC-ND 4.0)

Received Date : 23-December-2015 / Accepted: 10-May-2016 / Published Online: 30-March-2017

ARTICLE

ABSTRACT

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains compared to unexposed controls. AFLP showed a high discriminatory power, confirming the existence of different genotypes within the species and presence of DNA polymorphismsin UVA-exposed colonies.

Content not available PDF Share

FIGURES & TABLES

REFERENCES

Brunet-Galmés I., A. Busquets, A. Peña, M. Gomila, B. Nogales, E. García-Valdés, J. Lalucat, A. Bennasar and R. Bosch. 2012. Complete genome sequence of the naphthalene-degrading bacte-rium Pseudomonas stutzeri AN10 (CCUG 29243). J. Bacteriol. 194: 6642–6643.

 

Hensgens L.A., A. Tavanti, S. Mogavero, E. Ghelardi and S. Senesi. 2009. AFLP genotyping of Candida metapsilosis clinical isolates: evidence for recombination. Fungal Gen. Biol. 46: 750–758.

 

Grüntzig V., S.C. Nold, J. Zhou and J.M. Tiedje. 2001. Pseudomonas stutzeri nitrite reductase gene abundance in environmental samples measured by real-time PCR. Appl. Environ. Microbiol. 67: 760–768.

 

Krauss S.L. 2000. Accurate gene diversity estimates from amplified fragment length by polymorphism (AFLP) markers. Mol. Ecol. 9: 1241–1245.

 

Lalucat J., A. Bennasar, R. Bosch, E. García-Valdés and N.J. Palleroni. 2006. Biology of Pseudomonas stutzeri. Microbiol. Mol. Biol. Rev. 70: 510–547.

 

Lima A.B., L.S. Leão-Vasconcelos, M. Costa de, L.O. Vilefort, M.C. André, M.A. Barbosa and M.A. Prado-Palos. 2015. Pseudomonas spp. isolated from the oral cavity of healthcare workers from an oncology hospital in Midwestern Brazil. Rev Inst Med Trop Sao Paulo. 57: 513–514.

 

Santos A.L., V. Oliveira, I. Baptista, I. Henriques, N.C. Gomes,A. Almeida, A. Correia and A. Cunha. 2013. Wavelength dependence of biological damage induced by UV radiation on bacteria. Arch. Microbiol. 195: 63–74.

 

Sepúlveda-Torres L.C., J. Zhou, C. Guasp, J. Lalucat, D. Knaebel, J.L. Plank and C. Criddle. 2001. Pseudomonas sp. strain KC represents a new genomovar within Pseudomonas stutzeri. Int. J. Syst. Evol. Microbiol. 51: 2013–2019.

 

Sikorski J., J. Lalucat and W. Wackernagel. 2005. Genomovars 11 to 18 of Pseudomonas stutzeri, identified among isolates from soil and marine sediment. Intern. J. Syst. Evol. Microbiol. 55: 1767–1770.

 

Sikorski J., R. Rosselló-Mora and M.G. Lorenz. 1999. Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis. Syst. Appl. Microbiol. 22: 393–402.

 

Tavanti A., L.A. Hensgens, E. Ghelardi, M. Campa and S. Senesi. 2007. Genotyping of Candida orthopsilosis by AFLP reveals genetic diversity among independent isolates and strain maintenance within the same patient. J. Clin. Microbiol. 45: 1455–1462.

 

Vos P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman and M. Kuiper. 1995. AFLP:a new technique for DNA fingerprinting. Nucleic Acids Res. 23: 4407–4414.

 

Xiang S.R., M. Cook, S. Saucier, P. Gillespie, R. Socha, R. Scroggins and L.A. Beaudette. 2010. Development of amplified fragment length polymorphism-derived functional strain-specific markers to assess the persistence of 10 bacterial strains in soil microcosms. Appl. Environ. Microbiol. 76: 7126–7135.

 

EXTRA FILES

COMMENTS