Vital Staining of Bacteria by Sunset Yellow Pigment

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 1 (March 2017) > List of articles

Vital Staining of Bacteria by Sunset Yellow Pigment

Peng Gao / Chengming Sun / Yuxia Li / Xiaohua Zou / Xun Wu / Yan Ling / Caifu Luan / Huipeng Chen *

Keywords : bio-safety, pathogenic bacteria detection, sunset yellow, vital staining

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 1 , ISSN (Online) 2544-4646, DOI: 10.5604/17331331.1234999, March 2017

License : (CC BY-NC-ND 4.0)

Received Date : 24-March-2016 / Accepted: 07-August-2016 / Published Online: 30-March-2017

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ABSTRACT

In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture with sunset yellow. Our results suggest that sunset yellow is suitable pigment to dye microorganisms.

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Adler A., S. Navon-Venezia, J. Moran-Gilad, E. Marcos, D. Schwartz and Y. Carmeli. 2011. Laboratory and clinical evaluation of screening agar plates for detection of carbapenem-resistant Enterobacteriaceae from surveillance rectal swabs. J. Clin. Microbiol. 49: 2239–2242.

 

Choi C., E. Kuatsjah, E. Wu and S. Yuan. 2010. The effect of cell size on the burst size of T4 bacteriophage infections of Escherichia coli B23. J. Exp. Microbiol. Immun. 14: 85–91.

 

Chung K.T., S.E. Stevens and C.E. Cerniglia. 2008. The reduction of azo dyes by the intestinal microflora. Crit. Rev. Microbiol. 18: 175–190

 

Cohen H.W., R.M. Gould and V.W. Sidel. 2004. The pitfalls of bioterrorism preparedness: the anthrax and smallpox experiences. Am. J. Pub. Health. 94: 1667–1671.

 

Conrad T.M., M. Frazier, A.R. Joyce, B.-K. Cho, E.M. Knight, N.E. Lewis, R. Landick and B.Ø. Palsson. 2010. RNA polymerase mutants found through adaptive evolution reprogram Escherichia coli for optimal growth in minimal media. Proceedings of the National Academy of Sciences. 107: 20500–20505.

 

Cox S., C. Mann, J. Markham, H. Bell, J. Gustafson, J. Warmington and S. Wyllie. 2000. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J. Appl. Microbiol. 88: 170–175.

 

De Mey M., G.J. Lequeux, J. Maertens, C.I. De Muynck, W.K. Soetaert and E.J. Vandamme. 2008. Comparison of protein quantification and extraction methods suitable for E. coli cultures. Biologicals 36: 198–202.

 

Ferreira L., F. Sánchez-Juanes, M. González-Ávila, D. Cembrero- Fuciños, A. Herrero-Hernández, J.M. González-Buitrago and J.L. Muñoz-Bellido. 2010. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 48: 2110–2115.

 

Jünger M., W. Vautz, M. Kuhns, L. Hofmann, S. Ulbricht, J.L. Baumbach, M. Quintel and T. Perl. 2012. Ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria. Appl. Microbiol. Biotechnol. 93: 2603–2614.

 

Nevado J.B., J.R. Flores, C.G. Cabanillas, M.V. Llerena and A.C. Salcedo. 1998. Resolution of ternary mixtures of Tartrazine, Sunset Yellow and Ponceau 4R by derivative spectrophotometric ratio spectrum-zero crossing method in commercial foods. Talanta 46: 933–942.

 

Ngwa G., R. Schop, S. Weir, C. León-Velarde and J. Odumeru. 2013. Detection and enumeration of E. coli O157: H7 in water samples by culture and molecular methods. J. Microbiol. Meth. 92: 164–172.

 

Pérez-Díaz I. and R. McFeeters. 2009. Modification of azo dyes by lactic acid bacteria. J. Appl. Microbiol. 107: 584–589.

 

Poul M., G. Jarry, M.O. Elhkim and J.-M. Poul. 2009. Lack of genotoxic effect of food dyes amaranth, Sunset Yellow and tartrazine and their metabolites in the gut micronucleus assay in mice. Food Chem. Tox. 47: 443–448.

 

Salleh F.M., A.M. Al-Mekhlafi, A. Nordin, A.M. Yasin, H.M. Al- Mekhlafi and N. Moktar. 2011. Evaluation of gram-chromotrope kinyoun staining technique: its effectiveness in detecting micro-sporidial spores in fecal specimens. Diagn. Microbiol. Infect. Dis. 69:82–85.

 

Salleh F.M., T.S. Anuar, A.M.Yasin and N. Moktar. 2012. Wintergreen oil: a novel method in Wheatley’s trichrome staining technique. J. Microbiol. Meth. 91: 174–178.

 

Sasaki Y.F., S. Kawaguchi, A. Kamaya, M. Ohshita, K. Kabasawa, K. Iwama, K. Taniguchi and S. Tsuda. 2002. The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 519: 103–119.

 

Tucker J.B. 2003. Biosecurity: limiting terrorist access.

 

Zimmermann R., R. Iturriaga and J. Becker-Birck. 1978. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl. Env. Microbiol. 36: 926–935.

 

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