Vital Staining of Bacteria by Sunset Yellow Pigment


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 66 , ISSUE 1 (March 2017) > List of articles

Vital Staining of Bacteria by Sunset Yellow Pigment

Peng Gao / Chengming Sun / Yuxia Li / Xiaohua Zou / Xun Wu / Yan Ling / Caifu Luan / Huipeng Chen *

Keywords : bio-safety, pathogenic bacteria detection, sunset yellow, vital staining

Citation Information : Polish Journal of Microbiology. Volume 66, Issue 1, Pages 113-117, DOI:

License : (CC BY-NC-ND 4.0)

Received Date : 24-March-2016 / Accepted: 07-August-2016 / Published Online: 30-March-2017



In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture with sunset yellow. Our results suggest that sunset yellow is suitable pigment to dye microorganisms.

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