SEARCH WITHIN CONTENT
Citation Information : Polish Journal of Microbiology. Volume 66, Issue 1, Pages 131-134, DOI: https://doi.org/10.5604/17331331.1235002
License : (CC BY-NC-ND 4.0)
Received Date : 03-December-2015 / Accepted: 04-January-2017 / Published Online: 30-March-2017
We compared the susceptibility to viral infection of splenocytes, isolated from young versus old CBA mice, and evaluated the antiviral actions of lactoferrin in splenocytes infected with Encephalomyocarditis virus (EMCV). Recombinant mouse lactoferrin (rmLF) and bovine lactoferrin (bLF) were used. There were no differences in the susceptibility to EMCV infection in the studied age categories. Both types of lactoferrins were protective in young and old mice. The study confirmed the undisturbed viral resistance in old mice and the protective actions of lactoferrin in viral infection. The antiviral action of the homologous mouse lactoferrin was demonstrated for the first time.
Castle S.C., K. Uyemura, W. Crawford, W. Wong and T. Makinodan. 1999. Antigen presenting cell function is enhanced in healthy elderly. Mech. Ageing. Dev. 107: 137–145.
Carey M.A., J.A. Bradbury, Y.D. Rebolloso, J.P. Graves, D.C. Zeldinand D.R. Germolec. 2010. Pharmacologic inhibition of COX-1 and COX-2 in influenza A viral infection in mice. PLoS One. 5: e11610.
Dailey R.W., S.Y. Eun, C.E. Russell and L.A. Vogel. 2001. B cells of aged mice show decreased expansion in response to antigen, but are normal in effectors function. Cell Immunol. 214: 99–109.
Hayek M.G., C. Mura, D. Wu, A.A. Beharka, S.N. Han, K.E. Paulson, D. Hwang and S.N. Meydani. 1997. Enhanced expression of inducible cyclooxygenase with age in murine macrophages. J. Immu-nol. 159: 2445–2451.
Kruzel M.L., J.K. Actor, M. Zimecki, J. Wise, P. Płoszaj, S. Mirza, M. Kruzel, S.A. Hwang, X. Ba and I. Boldogh. 2013. Novel recombinant human lactoferrin: differential activation of oxidative stress related gene expression. J. Biotechnol. 168: 666–675.
Kogut I., J.L. Scholz, M.P. Cancro and J.C. Cambier. 2012. B cell maintenance and function in aging. Semin. Immunol. 24: 342–349.
Labeur M., V.E. Nahmod, S. Finkielman and E. Arzt. 1991. Lesions of the medial septal nucleus produce a long-lasting inhibition of T lymphocyte proliferation. Neurosci. Lett. 125: 129–132.
Picard-Jean F., S. Bouchard, G. Larivée and M. Bisaillon. 2014. The intracellular inhibition of HCV replication represents a novel mechanism of action by the innate immune Lactoferrin protein. Antiviral Res. 111: 13–22.
Pietrantoni A., C. Fortuna, M.E. Remoli, M.G. Ciufolini andF. Superti. 2015. Bovine lactoferrin inhibits Toscana virus infection by binding to heparan sulphate. Viruses 29:480–495.
Ouyang Q., W.M. Wagner, D. Voehringer, A. Wikby, T. Klatt,S. Walter, C.A. Müller, H. Pircher and G. Pawelec. 2003. Age-associated accumulation of CMV-specific CD8+ T cells expressing the inhibitory killer cell lectin-like receptor G1 (KLRG1). Exp. Gerontol. 38: 911–920.
Russo A.J., J.H. Howell, T. Han, P. Bealmear and M.H. Goldrosen. 1979. Isolation of T-cells, B-cells and macrophages by a two-stage adherence procedure. J. Clin. Lab. Immunol. 2: 67–72.
Sansoni P., R. Vescovini, F. Fagnoni, C. Biasini, P. Sansoni,R. Vescovini, F. Fagnoni, C. Biasini, F. Zanni, L. Zanlari andothers. 2008. The immune system in extreme longevity. Exp. Gerontol. 43: 61–65.
Scholz J.L., A. Diaz, R.L. Riley, M.P. Cancro and D. Frasca. 2013. A comparative review of aging and B cell function in mice and humans. Curr. Opin. Immunol. 25: 504–510.
Simioni P.U., E.H. Costa and W.M. Tamashiro. 2007. Aging reduces the primary humoral response and the in vitro cytokine production in mice. Braz. J. Med. Biol. Res. 40: 1111–1120.
Välimaa H., J. Tenovuo, M. Waris and V. Hukkanen. 2009. Human lactoferrin but not lysozyme neutralizes HSV-1 and inhibits HSV-1 replication and cell-to-cell spread. Virol. J. 6: 53.
Zhang Y., Y. Wang, X. Gilmore and K. Xu. 2002. An aged mouse model for RSV infection and diminished CD8(+) CTL responses. Exp. Biol. Med. (Maywood). 227: 133–140.
Zheng Y., W. Zhang, Q. Ye, Y. Zhou, W. Xiong, W. He, M. Deng, M. Zhou, X. Guo, P. Chen and others. 2012. Inhibition of Epstein-Barr virus infection by lactoferrin. J. Innate. Immun. 4: 387–398.
Zimecki M. and M.L. Kruzel. 2007. Milk-derived proteins and peptides of potential therapeutic and nutritive value. J. Exp. Ther. Oncol. 6: 89–106.