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Article | 14-October-2020

Rapid genotyping of the major alleles at the Duffy (FY) blood group locus using real-time fluorescence polymerase chain reaction

The Duffy blood group system has clinical importance due to involvement in transfusion reactions and hemolytic disease of the newborn. Recently, the molecular basis of the two alleles, FY*A and FY*B (125G>A), and the mutation situated in the promoter region of the FY gene (–33T>C), have been elucidated. In order to develop an accurate, easy, and rapid genotyping method, we describe a procedure using the LightCycler®. Samples from 53 Caucasian Portuguese blood donors and 7 black

Fernando M. Araújo, Christina Pereira, Ana Aleixo, Isabel Henriques, Fátima Monteiro, Elsa Meireles, Pedro Lacerda, Luis M. Cunha-Ribeiro

Immunohematology, Volume 17 , ISSUE 2, 42–44

Article | 14-October-2020

Blood group antigen profile predicted by molecular biology— use of real-time polymerase chain reaction to genotype important KEL,JK, RHD, and RHCE alleles

Fernando Manuel Ferreira Araújo, Christiana Pereira, Fátima Monteiro, Isabel Henriques, Elsa Meireles, Pedro Lacerda, Ana Aleixo, Regina Celeste, Luis M. Cunha-Ribeiro, Maria J. Rodrigues

Immunohematology, Volume 18 , ISSUE 3, 59–64

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