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  • Immunohematology

 

Report | 01-December-2019

RHCE variant allele: RHCE*ce254G,733G

A novel RHCE allele was identified in a 53-year-old AfricanAmerican female blood donor with an Rh phenotype of D+ C– E– c+ e+ and a negative antibody screen. The donor’s cells typed e+ with all antisera tested. By gel-based genotyping and cDNA analysis, the two RHCE alleles in this donor were characterized. One allele was found to be the known allele RHCE*01.20.01 (RHCE*ce733G) and the second was novel: RHCE*01.06.02 (RHCE*ce254G,733G).

Jessica A. Keller, Trina Horn, Colleen Chiappa, Camilla Melland, Christine Vietz, Lilian Castilho, Margaret A. Keller

Immunohematology, Volume 30 , ISSUE 3, 121–122

Report | 01-December-2019

Prevalence of RHD*DOL and RHCE*ce(818T)  in two populations

The alleles RHCE*ceBI (RHCE*ce 48C, 712G, 818T, 1132G) and RHCE*ceSM (RHCE*ce 48C, 712G, 818T) encode the lowprevalence Rh antigen STEM. These alleles frequently travel in cis with RHD*DOL. To estimate the frequency of these alleles, we tested a total of more than 700 samples in two populations. Blood samples were obtained from patients with sickle cell disease and from blood donors of African descent. DNA extractions and analyses were performed by standard methods. In the United States, none

Christine Halter Hipsky, Daiane Cobianchi da Costa, Ricardo Omoto, Angela Zanette, Lilian Castilho, Marion E. Reid

Immunohematology, Volume 27 , ISSUE 2, 66–67

Article | 14-October-2020

Blood group antigen profile predicted by molecular biology— use of real-time polymerase chain reaction to genotype important KEL,JK, RHD, and RHCE alleles

The most clinically important blood group systems in transfusion medicine, excluding the ABO system, are the RH, Kell, and Kidd systems. Alloantibodies to antigens of these systems may be produced following blood transfusion or during pregnancy and can result in serious hemolytic transfusion reactions and hemolytic disease of the newborn.We developed rapid and robust techniques for RHD, RHCE, KEL, and JK genotyping with the use of a real-time polymerase chain reaction instrument. Two

Fernando Manuel Ferreira Araújo, Christiana Pereira, Fátima Monteiro, Isabel Henriques, Elsa Meireles, Pedro Lacerda, Ana Aleixo, Regina Celeste, Luis M. Cunha-Ribeiro, Maria J. Rodrigues

Immunohematology, Volume 18 , ISSUE 3, 59–64

Report | 12-March-2020

RHCE*ceAR encodes a partial c (RH4) antigen

procedures. RBCs from the patient typed C+c+ but his plasma contained alloanti-c. DNA analyses showed the presence of RHCE*Ce in trans to RHCE*ceAR with RHD*D and RHD*Weak D Type 4.2.2. The amino acid changes on RhceAR are such that a C+c+ patient made alloanti-c. This case shows that RhceAR carries a partial c antigen and illustrates the value of DNA testing as an adjunct to hemagglutination to aid in antibody identification in unusual cases.

Marion E. Reid, Christine Halter Hipsky, Christine Lomas-Francis, Akiko Fuchisawa

Immunohematology, Volume 26 , ISSUE 2, 57–59

Report | 01-December-2019

Molecular background of RH in Bastiaan, the RH:–31,–34 index case, and two novel  RHD alleles

available reagents. We tested a cohort of African Americans to estimate the frequency of the RHCE*ce 48C, 733G, 1006T allele, and in addition found two novel RHD alleles. Hemagglutination tests and DNA analyses were performed by standard methods. Analyses revealed homozygosity for RHCE*ce 48C, 733G, 1006T in Bastiaan. RBCs from Bastiaan were strongly agglutinated by three commercial anti-e reagents. Testing RBCs from people homozygous for RHCE*ce 48C, 733G, 1006T showed that anti-e MS16, MS17, and MS63

Marion E Reid, Christine Halter Hipsky, Randall W. Velliquette, Christine Lomas-Francis, Kathleen Larimore, Coral Olsen

Immunohematology, Volume 28 , ISSUE 3, 97–103

Article | 15-April-2020

Serologic and molecular genetic management of a pregnancy complicated by anti-Rh18

Antibodies,such as anti-Rh18 (Hr/HrS),that react with the common products of RHCE can cause HDN as well as severe hemolytic transfusion reactions. Individuals with anti-Rh18 antibodies can have different RHCE genetic backgrounds;therefore,sera and RBCs from these individuals may cross-react. In these situations, genotyping may be the best method to determine compatibility. We report a 26-year-old pregnant Puerto Rican woman who presented at 31 weeks’gestation with anti-E and anti-Rh18 in

Richard L. Haspel, Dawn Michelle, Richard M. Kaufman, Sunitha Vege, Connie M. Westhoff

Immunohematology, Volume 22 , ISSUE 3, 132–135

Report | 25-March-2020

Rapid, single-subject genotyping to predict red blood cell antigen expression

isolated from fresh and 1- and 2-week-old stored blood from 20 donors with known ABO and Rh phenotypes and was used for ABO, RHD, and RHCE genotyping using SSPs.  The amplicons were analyzed using gel electrophoresis and a novel microfluidic onchip electrophoresis system.  Analysis of DNA from fresh and 1- and 2-week-old blood by SSP and gel electrophoresis yielded the correct ABO, RHD, and RHCE type in all samples, but with DNA from 2-week-old stored blood the amplicons were more difficult

Stefanie L. Slezak, Sharon Adams, Hallie Lee-Stroka, Joshua E. Martin, Lorraine Caruccio, David F. Stroncek

Immunohematology, Volume 24 , ISSUE 4, 154–159

Case report | 16-October-2019

A delayed and acute hemolytic transfusion reaction mediated by anti-c in a patient with variant RH alleles

RHCE*ceEK/ RHCE*ceAR alleles. The patient was previously alloimmunized to D, C, and E and possibly hrS. Further transfusion of D–C–E–K– RBCs resulted in a suspected acute hemolytic transfusion reaction and the subsequent identification of anti-c. Monocyte monolayer assay testing suggests clinical significance with a range of 29.5–38.5 percent reactive monocytes.

Tiffany K. Walters, Thomas Lightfoot

Immunohematology, Volume 34 , ISSUE 3, 109–112

Article | 18-October-2020

The Rh blood group system: the first 60 years of discovery

Christine Lomas-Francis, Marion E. Reid

Immunohematology, Volume 16 , ISSUE 1, 7–17

Article | 16-November-2020

Rhmod phenotype: a parentage problem solved by denaturing gradient gel electrophoresis of genomic DNA

support this hypothesis, DNA analysis of the RHD and RHCE genes was performed on the five family members. Polymerase chain reaction (PCR) products from exons 2 and 5 were analyzed by denaturing gradient gel electrophoresis (DGGE). The DNA results corroborated the serologic findings and refuted the exclusion of paternity.

Fiona J. Steers, Maura Wallace, Marialuisa Mora, Ben Carritt, Patricia Tippett, Geoff Daniels

Immunohematology, Volume 12 , ISSUE 4, 154–158

Case report | 14-October-2020

Moderate hemolytic disease of the newborn (HDN) due to anti-Rh17 produced by a black female with an e variant phenotype

cause mild to fatal HDN. We report an example of anti-Rh17 produced by a black female with an e variant RBC phenotype that caused moderate HDN. A panel of seven monoclonal anti-e demonstrated her RBCs carried a variant e antigen, and her genotype was RHD, RHce by PCR-RFLP analysis. Amniotic fluid with ΔOD450 values from 30 to 35 weeks’ gestation predicted moderate HDN probability by the Liley method. At 38+ weeks, a viable 3165 g female infant was delivered. The infant’s direct

Marla C. Brumit, Gary E. Carnahan, James R. Stubbs, Jill R. Storry, Marion E. Reid

Immunohematology, Volume 18 , ISSUE 2, 40–42

Case report | 25-March-2020

RHD deletion in a patient with chronic myeloid leukemia

primers designed to amplify RHD exons 3, 4, or 7.  Two assays that detect the hybrid Rhesus box showed deletion of RHD.  Amplification of RHCE in the patient’s DNA was as efficient as that of control samples, and multiplex and PCR-RFLP assays predicted her RBCs would be C–E–c+e+.  Based on finding a hybrid Rhesus box and absence of D-specific exons, we conclude that DNA from the patient’s WBCs carries a deleted RHD.  This explains the molecular mechanism

Ann Murdock, Deborah Assip, Kim Hue-Roye, Christine Lomas-Francis, Zong Hu, Sunitha Vege, Connie M. Westhoff, Marion E. Reid

Immunohematology, Volume 24 , ISSUE 4, 160–164

Article | 17-February-2021

Concordance of two polymerase chain reaction–based blood group genotyping platforms for patients with sickle cell disease

concordance tally. HEA BeadChip does not interrogate an SNV associated with expression of the Mia antigen; therefore, Mia was not included in the comparison. Likewise, LW, Scianna, and Cartwright antigens were not included in the analysis for discrepancies or in our concordance calculation. We found four discrepant results. Thus, the concordance rate was 99.9 percent (100 × [4275 − 4]/4275). All four discrepancies were in the Rh blood group system (Table 1). ID CORE XT identified the RHCE*ceAR allele in

C.A. Sheppard, N.L. Bolen, G. Meny, M. Kalvelage, G. Ochoa-Garay

Immunohematology, Volume 36 , ISSUE 4, 123–128

Article | 15-April-2020

Molecular characterization of GYPB and RH in donors in the American Rare Donor Program

referred were hrB– and carry at least one hybrid RHD-CE(3-7)-D gene that encodes a variant C antigen linked to RHCE*ceS that encodes the VS+V– phenotype. Surprisingly, the majority of donors were heterozygous, some even carrying conventional alleles, suggesting that the loss of expression of the hrB epitopes on RBCs is a dominant phenotype. Although antigen-matching of patients with SCD with donors for C, E, and K antigens has decreased the incidence of alloimmunization, some patients still

Sunitha Vege, Connie M. Westhoff

Immunohematology, Volume 22 , ISSUE 3, 143–147

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