the technique has already been applied to the detection of P. mirabilis (Liu et al. 2019). At present, 16S ribosomal RNA (rRNA) sequencing, selective media, biochemical identification, and serological tests remain the mainstay modalities for distinguishing between strains of P. mirabilis and P. vulgaris (O’Hara et al. 2000). In this study, we used a dual TaqMan Real-Time PCR for the rapid and accurate identification and classification of P. mirabilis and P. vulgaris in food samples.
Polish Journal of Microbiology , ISSUE 3, 293–300
three isolates of Leptospira and the reference strain was isolated and purified with and without shock using a hybrid-RTM (Gene ALL Korea-South Seoul) RNA extraction kit. cDNA synthesis was performed by the TwoStep kit HyperScriptTM first-strand synthesis kit (Gene ALL Korea, South Seoul). cDNA was used as a template for PCR and real-time PCR.
Real-Time PCR. The real-time PCR reaction was performed on Step One and Step One Plus Real-Time PCR systems (Applied Biosystems-Thermo Fisher Scientific
SONA ROSTAMPOUR YASOURI,
NAFISEH SADAT NAGHAVI,
Polish Journal of Microbiology , ISSUE 3, 301–310
. kumamotoensis are known to inhabit in many chrysanthemum growing areas (Iwahori et al., 2008) and Pratylenchus may cause chronic damage to chrysanthemum (Kobayashi, 1995). Chemical treatments including nematicides, such as fosthiazate, are popular means to control nematode damage in Okinawa, however, nematode diagnosis in a chrysanthemum field is not quick and easy. Koyama et al. (2016) reported a low cost and high-throughput approach to quantify Pratylenchus spp. with the real-time PCR method by using pre
Journal of Nematology , 1–11
This work presents results of the research on the occurrence of Coxiella burnetii and Francisella tularensis in the tissues of wild-living animals and ticks collected from Drawsko County, West Pomeranian Voivodeship. The real-time PCR testing for the pathogens comprised 928 samples of animal internal organs and 1551 ticks. The presence of C. burnetii was detected in 3% of wild-living animals and in 0.45–3.45% (dependent on collection areas) of ticks. The genetic sequences of F
Polish Journal of Microbiology , ISSUE 4, 529–534
The accurate diagnosis of Epstein-Barr virus (EBV) infections is important, as many other infectious agents or diseases can cause similar symptoms. In this study, sera of pediatric patients who were suspected to have an EBV infection, were sent to Eskisehir Osmangazi University Faculty of Medicine, Department of Clinical Microbiology, and investigated by IFA, ELISA, immunoblotting and Real-time PCR. The performances of these tests were compared with IFA. The rates of agreement between ELISA and
Ener Cagri Dinleyici,
Polish Journal of Microbiology , ISSUE 1, 81–88
Vovlas, 2007). Molecular techniques as RAPD-PCR and sequencing of D2 to D3 expansion segments of the 28S rRNA was used for the identification of P. vulnus on different plant species (Subbotin et al., 2008; Bakooie et al., 2012; Lopez-Nicora et al., 2012). Moreover, real-time PCR provides sensitive identification of the species with species-specific primers using 1/128 of the DNA of one nematode (Huang and Yan, 2017).
Pratylenchus vulnus (Allen and Jensen, 1951) (walnut root lesion nematode) has been
Mehmet Sait Karaca,
Ozlem Ates Sonmezoglu
Journal of Nematology , 1–4
Polish Journal of Microbiology , ISSUE 3, 377–382
intervals of 0, 30, 60, 90 and 120 min, using a spectrofluorometer (Shimadzu, Japan) with excitation at 485 nm and emission at 530 nm. All results were represented as an average of three biological samples.
Molecular quantification of the Candida efflux pump genes MDR1, CDR1, and CDR2 using quantitative real-time PCR. Quantitative real-time PCR was applied for two isolates C6 and C21 whose efflux pump activity was more prominently affected by amikacin using the Applied Biosystems 7500 Real-Time PCR
EVA A. EDWARD,
NELLY M. MOHAMED,
AZZA S. ZAKARIA
Polish Journal of Microbiology , ISSUE 1, 73–84
way and that genomic DNA solutions were free of PCR inhibitors. These methods revealed the presence of L. delbrueckii and S. thermophilus. The real-time PCR enabled quantification with a detection of 101–103 CFU/g of product. qPCR-standard curves were linear over seven log units down to 101 copies per reaction; efficiencies ranged from 77.9% to 93.6%. Cheese samples were analysed with plate count method and qPCR in parallel. Compared with the classic plate count method, the newly developed
Milena A. Stachelska
Polish Journal of Microbiology , ISSUE 4, 491–499
MILENA A. STACHELSKA
Polish Journal of Microbiology , ISSUE 2, –
EUI SHIK RHA
Polish Journal of Microbiology , ISSUE 2, 211–216
. HCV PB analysis indicated DxN VERIS combined = −0.3394 + 0.8602 Qiagen log IU/ml with the correlation of 0.90. HCV plots for PB and BAP are shown in Fig. 2.
HCV plots for Passing-Bablok (upper) and Bland Altman analysis (lower).
Viral nucleic acid detection is the gold standard for the detection of viral genomes in clinical samples. COBAS Ampliprep, artus Qiagen and Abbott real-time PCR assays are currently the most frequently used platforms worldwide in this field. DxN VERIS systems
Polish Journal of Microbiology , ISSUE 1, 139–143
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 341–352
with the appropriate drug-containing medium. On the seventh day, total RNA was extracted from both control and treatment groups, cDNA was synthesized using the mRNA extracted from each sample, and real-time PCR was performed.
Cell viability test
To determine the non-toxic doses of VPA (Darou Pakhsh Pharma. Chem. Co, Iran) and crocin (Puyesh Darou Sina, Iran), an MTT assay was performed. EPI-NCSCs were seeded in 96-well plates, at a density of 5×103 cells/well, 24 h prior to treatment. Then, EPI
Acta Neurobiologiae Experimentalis , ISSUE 1, 38–46
Polish Journal of Microbiology , ISSUE 1, 39–48
. mediasiatica isolated mostly in Asia and F. tularensis subsp. novicida, non-pathogenic to humans. Due to its ability to infect and variable forms of the disease, the etiological agent of tularaemia is classified by the CDC (Centers for Disease Control and Prevention, USA) as a biological warfare agent with a high danger potential (group A). The majority of data describing incidence of tularaemia in Poland is based on serological tests. However, real-time PCR method and MST analysis of F. tularensis highly
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 58–67
carried out in standard automated systems. Subsequently, FISH (Fluorescent In-Situ Hybridization) and nested multiplex-real-time-PCR (PCR) were performed. Blood cultures, FISH and PCR yielded positive results in 18%, 39.1%, and 71.7% of samples, respectively. Significant differences were found between the results obtained through culture before and after induction of antibiotherapy: 25.5% vs. 9.7%. There was no significant difference in FISH and PCR results in relation to antibiotics. The three
TOMASZ W. ŹRÓDŁOWSKI,
Polish Journal of Microbiology , ISSUE 4, 479–486