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  • Immunohematology



Trends of ABO and Rh phenotypes in transfusion-dependent patients in Pakistan

The objective of this study was to determine the prevalence of ABO and Rh phenotypes in the general Pakistan population. This information could be used to help reduce the rate of alloimmunization in patients with blood disorders, such as thalassemia major, who require frequent blood transfusions. A total of 242 patients with blood disorders requiring frequent blood transfusions were enrolled in the study. ABO and Rh typing was performed on samples from these patients using tube and gel methods

Nida Anwar, Munira Borhany, Saqib Ansari, Sana Khurram, Uzma Zaidi, Imran Naseer, Muhammad Nadeem, Tahir Shamsi

Immunohematology , ISSUE 4, 170–173

Research Article

Modelling of Temperature Coefficient of Resistance of a Thin Film RTDTowardsExhaust Gas Measurement Applications

Current research in the automotive industry sector focuses on the application of platinum thin film sensors for exhaust temperature measurement. The prime intention of this paper is to pioneer the design and development of Platinum/Rhodium (Pt/Rh) sensors for exhaust gas temperature measurement. The developed sensors were able to endure harsh temperature environments (up to 950º C). In addition, the Pt/Rh sensors resistivity response to temperature increase was described by a second order

Anthony Maher, Vijayalakshmi Velusamy, Daniel Riordan, Joseph Walsh

International Journal on Smart Sensing and Intelligent Systems , ISSUE 5, 1–4

Case report

Two cases of the variant RHD*DAU5 allele associated with maternal alloanti-D  

Rh is a complex blood group system with diverse genotypes that may encode weak and partial D variants. Standard serologic analysis may identify clinically significant D variants as D+; nevertheless, individuals with these D variants should be managed as D– patients to prevent antibody formation to absent D epitopes. Variant identification is necessary during pregnancy to allow for timely and appropriate Rh immune globulin (RhIG) prophylaxis for hemolytic disease of the fetus and newborn

Jennifer A. Duncan, Susan Nahirniak, Rodrigo Onell, Gwen Clarke

Immunohematology , ISSUE 2, 60–63


Distribution of blood groups in the Iranian general population

We report the first study of antigen and phenotype prevalence within various blood group systems in the Iranian general population. In this retrospective study, samples from 3475 individuals referred to the Immunohematology Reference Laboratory of the Iranian Blood Transfusion Organization, Tehran, Iran, for paternity testing from 1998 to 2008 were additionally tested for red blood cell (RBC) antigens in the Rh, Kell, Kidd, Duffy, MNS, Lutheran, P1PK, and Xg blood group systems. The antigen

Ehsan Shahverdi, Mostafa Moghaddam, Ali Talebian, Hassan Abolghasemi

Immunohematology , ISSUE 4, 135–139


Laboratory management of perinatal patients with apparently “new” anti-D

Despite the existence of long-standing, well-organized programs for Rh immune globulin (RhIG) prophylaxis, immune anti-D continues to be detected in the D– perinatal population. Between 2006 and 2008, 91 prenatal patients, found to have a previously unidentified anti-D, were followed up with a survey to their treating physician and with additional serologic testing where possible. The physician survey requested pregnancy and RhIG history information, including recent or distant potential

Judith L. Hannon, Gwen Clarke

Immunohematology , ISSUE 3, 108–111

Research Article

Deterministic Blockmodeling of Two-Mode Binary Networks Using a Two-Mode KL-Median Heuristic

Deterministic blockmodeling of a two-mode binary network matrix based on structural equivalence is a well-known problem in the social network literature. Whether implemented in a standalone fashion, or embedded within a metaheuristic framework, a popular relocation heuristic (RH) has served as the principal solution tool for this problem. In this paper, we establish that a two-mode KL-median heuristic (TMKLMedH) seeks to optimize the same criterion as the RH for deterministic blockmodeling. The

Michael Brusco, Hannah J. Stolze, Michaela Hoffman, Douglas Steinley, Patrick Doreian

Journal of Social Structure , ISSUE 1, 1–22



using the Layer-by-Layer (LbL) technique. The refractive index of this polymeric coating is sensitive to changes in the external relative humidity (RH), which permits the fabrication of humidity sensors based on LMRs.The wavelength based fabricated sensors showed a dynamical range of 65 nm when the RH varied in the range from 20 to 80% and it has a good linearity when the RH is higher than 40%, high stability and are highly reproducible.

M. Hernaez, C.R. Zamarreño, I. Del Villar, F.J. Arregui, I.R. Matias

International Journal on Smart Sensing and Intelligent Systems , ISSUE 4, 653–660


Humidity sensor based on silver nanoparticles embedded in a polymeric coating

sensitivity to Relative Humidity (RH) variations, suitable for high performance applications such as human breathing monitoring.

Pedro J. Rivero, Aitor Urrutia, Javier Goicoechea, Francisco J. Arregui, Ignacio R. Matías

International Journal on Smart Sensing and Intelligent Systems , ISSUE 1, 71–83


Red blood cell phenotype prevalence in blood donors who self-identify as Hispanic

populations. We performed a retrospective review of all serologic and molecular typing from donors who self-reported as Hispanic. The phenotype prevalence was reported and compared with rates from other racial/ethnic groups. A total of 1127 donors who selfidentified as Hispanic were screened by serologic methods for Rh and Kell antigens, and 326 were subsequently selected for molecular typing. The most prevalent probable Rh phenotypes were R1r (26.6%), R1R2 (21.5%), and R1R1 (20.7%); rr was found in 7.8

Chelsea A. Sheppard, Nicole L. Bolen, Beth Eades, Gorka Ochoa-Garay, Mark H. Yazer

Immunohematology , ISSUE 3, 119–124


Dithiothreitol treatment of red blood cells

to a 2–5 percent suspension in PBS. Test DTT-treated cells with serum containing the antibody in question. Test K+ treated RBCs with anti-K. DTT = dithiothreitol; PBS = phosphate-buffered saline; RBCs = red blood cells. Treatment of autologous RBCs with 0.01 M DTT can resolve spontaneous agglutination due to potent IgM autoantibodies that interfere with ABO/Rh and direct antiglobulin testing.4 Monoclonal anti-CD38 (daratumumab), approved by the U.S. Food and Drug Administration for

C.B. Bub

Immunohematology , ISSUE 4, 170–172



ultra-thin coatings have been fabricated with the Layer-by-Layer (LbL) technique using different diameter SiO2 nanoparticles. These coatings were characterized to optimize their behavior and were applied to optical fiber substrates in order to create superhydrophilic optical interferometric cavities. These cavities show an optical response to Relative Humidity (RH) variations that are suitable for high performance sensing applications such as human-breathing monitoring.

Miguel Hernaez, Ignacio R. Matias, Jesus M. Corres, Javier Goicoechea, Javier Bravo, Francisco J. Arregui

International Journal on Smart Sensing and Intelligent Systems , ISSUE 1, 63–74



with improved thermal stability of coating material in wide range of 9-95 % RH with good process repeatability. Sensor response is also observed to be very fast and highly reversible. Advantage of our developed composite material become evident when it exhibits wider range of moisture sensitivity compare to pure PVA or pure TiO2 material found in literature. Performance of PVA-TiO2 nanocomposite thick film is also evaluated by capacitance method and result found to agree with coated modified clad

Chandra Khatuaa, Ipsita Chinyaa, Debdulal Sahab, Shyamal Das, Ranjan Sen, Anirban Dhar

International Journal on Smart Sensing and Intelligent Systems , ISSUE 3, 1424–1442



Fermentation is extremely a crucial process which is primarily responsible for tea quality. It is an oxidation process where tea leaves change colour and smell. Relative humidity (RH) and temperature are two important physical parameters which play a crucial role in producing good quality tea. This work is an attempt to develop and implement a monitoring system for fermentation room of tea factory. Due to the larger dimension of the fermentation room, it requires several numbers of monitoring

Debashis Saikia, P.K. Boruah, Utpal Sarma

International Journal on Smart Sensing and Intelligent Systems , ISSUE 3, 1254–1270

Research Article

NTC Thermistors of Y-Al-Mn-Fe-Ni-Cr-O Ceramics for Wide Temperature Range Measurement

NTC thermistors of Y-Al-Mn-Fe-Ni-Cr-O systems were fabricated by using normal ceramic processing for wide temperature range measurement. Pt-Rh alloy as electrodes was inserted into the body during the forming process to increase the reliability of high temperature and to decrease the contact resistance. The properties were analyzed by XRD, SEM and resistance measurement. There are no distinct XRD patterns between Y0.2Al0.1Mn0.27Fe0.16Ni0.27Ox and Y0.2Al0.1Mn0.264Fe0.16 Ni0.264Cr0.012Ox because

Woonyoung Lee, Jinseong Park

International Journal on Smart Sensing and Intelligent Systems , ISSUE 5, 1–4

Original Paper

Neurobiological and clinical predictors of remission and antiepileptic treatment efficacy in partial epilepsies

the dependent variables was observed; and some combinations may be used for prediction purposes of therapeutic remission and percentage in seizure reduction. The FLE in the men with the right-handedness and the left focus (FLE • M • Rh • LF) resulted in the maximal length of therapeutic remission and maximal seizures reduction, while the other combinations have resulted in less favorable treatment results. The TLE • M • Rh • RF and TLE • M • Lh • LF

Vladimir V. Kalinin, Anna A. Zemlyanaya, Elena V. Zheleznova, Lyudmila V. Sokolova

Journal of Epileptology , ISSUE 1, 15–25

Case report

Autoanti-C in a patient with primary sclerosing cholangitis and autoimmune hemolytic anemia: a rare presentation

-reactive AIHA. Further testing showed the possibility of anti-C. The patient’s Rh phenotype was C+D+E–c– e+. Further testing with select cells, serial alloadsorption, and an elution confirmed anti-C specificity. The patient was transfused with two C–, crossmatch-compatible packed red blood cell units. The patient’s hemoglobin level and general condition showed improvement. This unique case report shows PSC associated with AIHA caused by autoanti-C. Usually, warm AIHA

Meenu Bajpai, Ashish Maheshwari, Shruti Gupta, Chhagan Bihari

Immunohematology , ISSUE 3, 104–107


Fetal cardiac function by three-dimensional ultrasound using 4D-STIC and VOCAL – an update

malformations. In recent years, functional echocardiography has been used to study fetuses with a structurally normal heart but who are susceptible to hemodynamic changes due to the presence of extra-cardiac conditions, including, among others, fetal growth restriction, tumors/masses, twin-to-twin transfusion syndrome, fetal anemia (Rh alloimmunization), congenital infections, or maternal diseases such as diabetes mellitus, systemic arterial hypertension, and Graves’ disease(8–11). The assessment of cardiac

Nathalie Jeanne Bravo-Valenzuela, Alberto Borges Peixoto, Milene Carvalho Carrilho, Ana Letícia Siqueira Pontes, Caroline Cevante Chagas, Christiane Simioni, Edward Araujo Júnior

Journal of Ultrasonography , ISSUE 79, 287–294

Original Paper

Recovery of Gram-Positive Cocci and Candida albicans from Peroxygen/Silver-Based Disinfectants

; Staphylococcus aureus (ATCC 6538), Kucoria rhizophila (ATCC 9341) and Can­dida albicans (ATCC 10231) and Staphylococcus capitis as an environmental monitoring (EM) isolate sample (identified by miniaturized biochemical identification system). Disinfectants preparation, dilutions and the test procedure were carried on in laboratory under condi­tions simulating the working environment of 20–25°C and RH% 40–60. In-house made neutralizing broth was mixed with biocidal agents to make two

Mostafa E. Eissa, Ahmed S. Nouby

Polish Journal of Microbiology , ISSUE 1, 13–21


Stability guidelines for dithiothreitol-treated red blood cell reagents used for antibody detection methods in patients treated with daratumumab

14 days for observation of hemolysis. In Set 1, all antigen reactivity remained at ≥2+ with both single- and double-dose cells for 14 days. The Rh antigens gave stronger reactions longer, compared with those tested in the Duffy, Kidd, and MNS blood group systems. Sets 2 and 3 were monitored for hemolysis. On day 3, Set 2 began displaying hemolysis, with complete hemolysis by day 8. Set 3 did not display hemolysis in 14 days. In conclusion, a large volume of RBCs can be treated with DTT and

Wendy L. Disbro

Immunohematology , ISSUE 3, 105–109


Assessment of common red blood cell pretreatments to yield an accurate serologic antigen phenotype compared with genotype-predicted phenotype

EGA treatment in removing IgG from RBCs. This study aimed to compare the accuracy of the results obtained from the commonly used RBC pretreatment methods with the results from a genotype-predicted phenotype using the BeadChip platform. Table 1. Blood group antigens predicted by the HEA BeadChip genotyping panel Blood group system Antigens RH C, c, E, e, V, VS FY Fya, Fyb, Fyb silenced DO Doa, Dob, Hy, Joa SC Sc1, Sc2 DI Dia, Dib LW LWa, LWb CO Coa, Cob JK Jka, Jkb KEL

T. Horn, J. Hamilton, J. Kosanke, V.W. Hare, W. Kluver, W. Beres, S. Nance, M.A. Keller

Immunohematology , ISSUE 4, 147–151

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