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Article

Separation of multiple antibodies by adsorption with allogeneic red blood cells

Principle Antibody detection and identification are processes that are commonly performed in the transfusion service before the transfusion of allogeneic red blood cells (RBCs). Antibody identification usually follows the discovery of a positive antibody detection test, or other factors such as ABO serum/cell discrepancy or incompatible crossmatch.1 Antibody identification is a necessary practice in blood banking to determine blood products that are suitable for transfusion to an individual

E.M. Ekema

Immunohematology , ISSUE 4, 155–158

Article

Applications of selected cells in immunohematology in a developing country: case studies

When an antibody is detected, its specificity should be determined and its likely clinical significance should be assessed. When one antibody has been identified, it becomes necessary to confirm the presence of additional significant antibodies to ensure that compatible blood is provided to the patient. To perform this confirmation, specific reagent red blood cells (RBCs) are selected; these are called selected cells. Though the most common use of selected cells is for antibody confirmation

Ravi C. Dara Dara, Aseem Kumar Tiwari, Dinesh Arora, Subhasis Mitra, Geet Aggarwal, Devi Prasad Acharya, Gunjan Bhardwaj

Immunohematology , ISSUE 1, 27–35

Review

How to recognize and resolve reagentdependent reactivity: a review

Reagent-dependent reactivity can be described as agglutination of red blood cells (RBCs) in serologic testing that is not related to the interaction of RBC antigens and antibodies that the test system is intended to detect. In other words, reagent-dependent reactivity results in false-positive agglutination reactions in serologic testing. These false-positive reactions can cause confusion in antigen typing and RBC antibody detection and identification procedures, and may result in delays in

Gavin C. Patch, Charles F. Hutchinson, Nancy A. Lang, Ghada Khalife

Immunohematology , ISSUE 3, 96–99

Article

Anti-Vel alloimmunization and severe hemolytic disease of the fetus and newborn

therapy has been noted to date. We report such a case recently encountered at our Fetal Center. Case Report The patient was a 31-year-old woman (gravida/para/abortus [GPA] = G2P1000) who was referred to our Fetal Center from a neighboring state for evaluation of Vel alloimmunization. Her past history was significant, with the finding of a positive antibody detection test and identification of anti-Vel during her previous pregnancy. Based on literature reports of only mild-to-moderate HDFN in

K.J. Moise, Y. Morales, M.F. Bertholf, S.N. Rossmann, Y. Bai

Immunohematology , ISSUE 4, 152–154

Report

Stability guidelines for dithiothreitol-treated red blood cell reagents used for antibody detection methods in patients treated with daratumumab

Daratumumab (DARA), a drug used to treat patients with multiple myeloma, causes interference in pre-transfusion testing. Samples from patients receiving DARA exhibit panreactivity in antibody detection and identification tests with red blood cells (RBCs). Many hospitals are sending these samples to reference laboratories. Dithiothreitol (DTT), a sulfhydryl chemical treatment of RBCs, negates this reactivity. This study investigated the stability of the antigens on DTT-treated RBCs to determine

Wendy L. Disbro

Immunohematology , ISSUE 3, 105–109

Article

Clinical and laboratory profile of anti-M

an ABO group discrepancy. If the antibody is active at 37°C, then M–, compatible RBCs must be transfused.7 We report here anti-M cases with varied presentations detected in our laboratory. Materials and Methods This report is from a tertiary care hemato-oncology center in eastern India treating patients with hematologic malignancies and solid tumors (i.e., gynecologic, gastrointestinal, head and neck, and soft tissue tumors). Pretransfusion antibody detection testing and crossmatch is performed

D. Basu, S. Basu, M. Reddy, K. Gupta, M. Chandy

Immunohematology , ISSUE 4, 165–169

Case report

Hemolytic transfusion reaction attributable to anti-Dia  

In situations when a patient's antibody detection test is negative, many institutions have moved from an indirect antiglobulin test (IAT) crossmatch to an electronic crossmatch system. Here we report a case of an acute hemolytic transfusion reaction attributable to anti-Dia in a patient with a negative antibody detection test. A 22-year-old female patient with a diagnosis of β thalassemia and sickle cell anemia commenced a routine exchange transfusion of 5 units of red blood cells

Arthur J. Joyce, Kelli M Quantock, Ray Banh, Yew-Wah Liew

Immunohematology , ISSUE 1, 6–8

original-paper

Presence of Antibodies Against Leptospira interrogans Serovar hardjo in Serum Samples from Cattle in Ukraine

. reported in 1981 that a combined random survey of both beef and dairy cattle in Northern Ireland resulted in positive antibody titers in 34.7% of the population sampled toward serovar hardjo using the MAT assay (Ellis et al. 1981). The serological herd prevalence of serovar hardjo in beef herds in England was 72% in 1987 (Pritchard et al. 1987); herd prevalence was 11% among beef herds in 2001 in Spain (Alonso-Andicoberry et al. 2001); and in the USA 42% of suckler herds were infected with Leptospira

ANTON PYSKUN, VITALII UKHOVSKYI, OLENA PYSKUN, VITALII NEDOSEKOV, VYACHESLAV KOVALENKO, SERHIY NYCHYK, MYKOLA SYTIUK, WOJCIECH IWANIAK

Polish Journal of Microbiology , ISSUE 3, 295–302

review-article

Neuromyelitis optica spectrum disorder: an overview

several years, NMO was considered to be a variant of the disease multiple sclerosis (MS), a CNS autoimmune disorder that results in severe demyelination. In 2004, antibody specific for the water channel protein called aquaporin 4 (AQP4) was found to cause NMO which led to NMO being identified as a separate disease (Lennon et al., 2004). Initially when identified, the disease was thought to present necrotic and demyelinating lesions only in the optic nerve and the spinal cord (Devic, 1894). So NMO was

Pooja Ramakrishnan, Devipriya Nagarajan

Acta Neurobiologiae Experimentalis , ISSUE 3, 256–272

Case report

ABO serology in a case of persistent weak A in a recipient following a group O–matched unrelated bone marrow transplant

Dianne E. Grey, Elizabeth A. Fong, Catherine Cole, Jesper Jensen, Jill Finlayson

Immunohematology , ISSUE 3, 99–104

Research Article

ADAPTIVE DYNAMIC CLONE SELECTION NEURAL NETWORK ALGORITHM FOR MOTOR FAULT DIAGNOSIS

A fault diagnosis method based on adaptive dynamic clone selection neural network (ADCSNN) is proposed in this paper. In this method the weights of neural network is encoded as the antibody, and the network error is considered as the antigen. The algorithm is then applied to fault detection of motor equipment. The experiments results show that the fault diagnosis method based on ADCS neural network has the capability in escaping local minimum and improving the algorithm speed, this gives better

Wu Hongbing, Lou Peihuang, Tang Dunbing

International Journal on Smart Sensing and Intelligent Systems , ISSUE 2, 482–504

Case report

Two cases of the variant RHD*DAU5 allele associated with maternal alloanti-D  

Rh is a complex blood group system with diverse genotypes that may encode weak and partial D variants. Standard serologic analysis may identify clinically significant D variants as D+; nevertheless, individuals with these D variants should be managed as D– patients to prevent antibody formation to absent D epitopes. Variant identification is necessary during pregnancy to allow for timely and appropriate Rh immune globulin (RhIG) prophylaxis for hemolytic disease of the fetus and newborn

Jennifer A. Duncan, Susan Nahirniak, Rodrigo Onell, Gwen Clarke

Immunohematology , ISSUE 2, 60–63

Research Article

TICK-BORNE PATHOGENS IN INDIVIDUALS WITH HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1) INFECTION

The studies on the occurrence and diversity of tick-borne infections in HIV-infected individuals have been few, and the subject has been relatively neglected when compared with other infections associated with HIV. Non-specific symptoms of tick-borne diseases pose a challenge in clinical care and may lead to misdiagnosis, especially in HIV-positive patients, who often experience many non-specific clinical symptoms. Additionally, in immunocompromised patients, a significant delay of antibody

Renata Welc-Falęciak, Małgorzata Bednarska, Magdalena Szatan, Agnieszka Pawełczyk

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 251–259

Short Communication

IgG Avidity: an Important Serologic Marker for the Diagnosis of Tick-Borne Encephalitis Virus Infection

21.3% patients without measurable IgM antibodies current/recent infection was confirmed by AI. IgG avidity represents an additional serologic marker that improves diagnosis of TBEV, especially in cases of atypical antibody response.

Tatjana Vilibic-Cavlek, Ljubo Barbic, Vladimir Stevanovic, Goranka Petrovic, Gordana Mlinaric-Galinovic

Polish Journal of Microbiology , ISSUE 1, 119–121

Original Paper

A field analysis trial comparing the turnaround times of routine and STAT red blood cell immunohematology testing

-transfusion tests and antibody titers. We found that the capacity of the VISION to load and run new samples, even while several other tests were ongoing, allowed for faster overall TAT when compared with samples run on the Echo. The PTs of the two analyzers (from load to result) were also compared, and we found them to be equivalent. These findings highlight the inherent flaw in considering only PT when assessing a laboratory’s ability to efficiently and consistently make results available to best

Katie Sackett, Andrea Kjell, Abigail M. Schneider Schneider, Claudia S. Cohn Cohn

Immunohematology , ISSUE 1, 1–5

Original Paper

Modeling alloantibody formation to highincidence red blood cell antigens in immune responders using genotypic data  

;, Immucor, Warren, NJ) was performed on all patient specimens referred for molecular testing over 45 months; serologic and clinical data were analyzed. We used simple and multiple logistic regression to model the risk factors for alloimmunization to an HIA. Of the 2591 patients genotyped, 32 (1.2%) were homozygous for at least one variant predicting absence of an HIA. Of these 32 patients, prior transfusion or pregnancy history was available for 29 (91%). Four susceptible patients made an antibody to an

Patricia A.R. Brunker, Keerthana Ravindran, R. Sue Shirey

Immunohematology , ISSUE 1, 9–14

Case report

A suspected delayed hemolytic transfusion reaction mediated by anti-Joa

A 32-year-old African-American woman with a history of sickle cell disease presented for surgical evaluation of left total hip arthroplasty due to avascular necrosis of the femoral head. In anticipation of a complex orthopedic procedure, pre-surgical blood work was ordered. The patient’s Fenwal blood sample typed as group O, D+. Although the patient had a history of anti-Fya, the antibody identification was inconclusive, so the workup was sent to a reference laboratory. The patient was

Ryan P. Jajosky, Wendy C. Lumm, Scott C. Wise, Roni J. Bollag, James F. Shikle

Immunohematology , ISSUE 2, 73–75

original-paper

Clinical Interpretation of Detection of IgM Anti-Brucella Antibody in the Absence of IgG and Vice Versa; a Diagnostic Challenge for Clinicians

course, the detection of specific IgM antibody in the absence of specific IgG antibody might be confusing for treating physician and therefore risks misdiagnosis of active brucellosis. Likewise, clinical interpretation of Brucella-specific IgG antibodies in the absence of IgM also creates confusion for clinicians. While some studies conducted on the diagnostic accuracy of ELISA mentioned a combined specificity of 100% for Brucella-specific IgM and IgG (Özdemir et al. 2011; Asaad et al. 2012), some

REEM AL JINDAN, NIDA SALEEM, AAMIR SHAFI, SHEIKH MUHAMMAD AMJAD

Polish Journal of Microbiology , ISSUE 1, 51–57

Case report

Autoanti-C in a patient with primary sclerosing cholangitis and autoimmune hemolytic anemia: a rare presentation

presents with a panreactive pattern, and it is difficult to find compatible blood. In this rare case, we could determine the specific antibody; efforts should always be made in cases of AIHA to identify the specificity of autoantibody.

Meenu Bajpai, Ashish Maheshwari, Shruti Gupta, Chhagan Bihari

Immunohematology , ISSUE 3, 104–107

Review

The Augustine blood group system, 48 years in the making

The high-prevalence antigen, Ata, was first identified in 1967, but it was not until 2015 that Ata became AUG1 of a new blood group system, Augustine (AUG). The new system was established after the identification of the gene encoding Ata and the recognition of a null phenotype (AUG:–1,–2) in an At(a–) patient with an antibody (anti-AUG2) reactive with At(a–) red blood cells. The At(a–) phenotype is very rare and, with the exception of the one family with the null

Geoffrey Daniels

Immunohematology , ISSUE 3, 100–103

Case report

A LU:−16 individual with antibodies

investigation was requested. This time, an antibody directed at a high-prevalence Lutheran antigen was found in addition to the anti-Lea and -Lub previously observed. Her serum was compatible with three out of five Lu(a−b−) reagent red blood cells (RBCs). One of the incompatible Lu(a−b−) reagent RBCs was known to be In(Lu) (KLF1 mutation). The genetic background of the other reagent RBC was unknown. The LU cDNA sequence analysis revealed the presence of the c.230G>A (Lua), c.679C

Carole Éthier, Cynthia Parent, Anne-Sophie Lemay, Nadia Baillargeon, Geneviève Laflamme, Josée Lavoie, Josée Perreault, Maryse St-Louis

Immunohematology , ISSUE 3, 110–113

Report

Trends of ABO and Rh phenotypes in transfusion-dependent patients in Pakistan

(37.7%), R1r (33.4%), R1R2 (19.4%), R2r (5.2%), and rr (4.3 %). All of the D– patients were rr. In our study, the highest prevalence of ABO phenotypes was group O and the most prevalent Rh antigen was e. Rh phenotyping, along with antibody screening and identification should be performed prior to transfusion of patients requiring multiple transfusions to reduce and possibly prevent the rate of alloimmunization.

Nida Anwar, Munira Borhany, Saqib Ansari, Sana Khurram, Uzma Zaidi, Imran Naseer, Muhammad Nadeem, Tahir Shamsi

Immunohematology , ISSUE 4, 170–173

Research paper

Upregulation of CCL3/MIP‑1alpha regulated by MAPKs and NF‑kappaB mediates microglial inflammatory response in LPS‑induced brain injury

and phosphorylation of MAPKs in the brains of rats 6, 24, and 72 h after LPS administration. Additionally, LPS‑treated rats were administered an anti‑MIP‑1alpha neutralizing antibody, and the microglial reaction and the expression of both cyclooxygenase‑2 and inducible nitric oxide synthase (iNOS) were analyzed. We finally evaluated the effect of an inhibitor of P38 MAPK, an inhibitor of ERK1/2, or an inhibitor of NF‑kappaB, on the levels of CCL3/MIP‑1alpha protein and numbers of microglia in the

Xiaobo Zhu, Dee Wei, Ou Chen, Zhaohua Zhang, Jiang Xue, Shanying Huang, Weiwei Zhu, Yibiao Wang

Acta Neurobiologiae Experimentalis , ISSUE 4, 304–317

short-communication

Evaluation of a Salmonella Strain Isolated from Honeybee Gut as a Potential Live Oral Vaccine Against Lethal Infection of Salmonella Typhimurium

0.5 HE13 0.2 0.2 0.5 0.5 0.5 0.5 HR1 = Group 1 of rabbits given antigen, C = control group given normal saline Microtiter plate agglutination test. This modified assay test was performed to check the antibody titer levels resultant from the antiserum reacting with the different Salmonella antigens (Barsoum and Awad 1972). It was performed in a 96-well microtiter plate in a triplicate with average readings presented in the Results section. A volume of 50 μl of tryptic soy broth was layered

HASSAN ZAFAR, SAJJAD UR RAHMAN, SULTAN ALI, MUHAMMAD TARIQ JAVED

Polish Journal of Microbiology , ISSUE 2, 173–183

Original Paper

Use of standard laboratory methods to obviate routine dithiothreitol treatment of blood samples with daratumumab interference

Daratumumab is an antibody currently used in the treatment of patients with refractory multiple myeloma. Blood samples from patients being treated with daratumumab may show panreactivity during pre-transfusion testing. To facilitate the provision of blood components for such patients, it is recommended that a baseline phenotype or genotype be established prior to starting treatment with daratumumab. If patient red blood cells (RBCs) require phenotyping after the start of daratumumab treatment

Nicholas J. Lintel, Debra K. Brown, Diane T. Schafer, Farai M. Tsimba-Chitsva, Scott A. Koepsell, Sara M. Shunkwiler

Immunohematology , ISSUE 1, 22–26

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