Original Paper
Stenotrophomonas maltophilia, an opportunistic pathogen usually connected with healthcare-associated infections, is an environmental bacterium. Intrinsic resistance to multiple antibiotics, with different virulence determinants in the last decade classified this bacterium in the group of global multiple drug resistant (MDR) organism. S. maltophilia clinical isolates, were collected from tertiary care pediatric hospital in Belgrade, Serbia to investigate influence of different factors on biofilm
Marjan Biočanin,
Haowa Madi,
Zorica Vasiljević,
Milan Kojić,
Branko Jovčić,
Jelena Lozo
Polish Journal of Microbiology , ISSUE 4, 433–438
original-paper
2012b; Kimura et al. 2017; Qin et al. 2017), and the ability to adhere to biotic and abiotic surfaces and/or to form biofilms (Kwaszewska et al. 2006; Souza et al. 2015a; Souza et al. 2015b; Qin et al. 2017; Kang et al. 2018).
The biofilm-producing bacteria are protected both against antibiotics (Fux et al. 2005; Lebeaux et al. 2014), and the host innate and adaptive immune responses (Souza et al. 2015b). Biofilm infections are associated with simultaneous activation of both arms of the host immune
ALINA OLENDER,
AGNIESZKA BOGUT,
AGNIESZKA MAGRYŚ,
JACEK TABARKIEWICZ
Polish Journal of Microbiology , ISSUE 4, 457–464
Original Paper
Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of
Rambha K. Shah,
Zhao H. Ni,
Xiao Y. Sun,
Guo Q. Wang,
Fan Li
Polish Journal of Microbiology , ISSUE 4, 501–508
Original Paper
antibiotic susceptibility test was done using the diffusion method. Biofilm formation was evaluated with microplate method using TTC. Bacteremia caused by Proteus spp. was found in 97 patients, mainly secondary to urinary tract infection. Most of the strains were susceptible to piperacillin with tazobactam (95.9%) and amikacin (86.7%). Elderly patients have a higher risk of mortality after BSIs caused by Proteus spp. A detailed analysis was made for randomly chosen 26 strains isolated from 11 patients
JOANNA KWIECIŃSKA-PIRÓG,
KRZYSZTOF SKOWRON,
EUGENIA GOSPODAREK-KOMKOWSKA
Polish Journal of Microbiology , ISSUE 4, 471–478
Short Communication
The aim of this study was to investigate the capacity of Streptococcus suis strains to form biofilms and to evaluate the antimicrobial activity of Penicillin G and N-acetylcystein (NAC) on both S. suis sessile and planktonic forms. Only non-typeable isolates of S. suis were correlated with a greater biofilm formation capacity. The MCI of Penicillin G and NAC required for inhibiting biofilm growth were higher than the required concentration for inhibiting planktonic growth. The combinations of
Ivette Espinosa,
Michel Báez,
Evelyn Lobo,
Siomara Martínez,
Marcelo Gottschalk
Polish Journal of Microbiology , ISSUE 1, 105–109
Original Paper
Abstract
Non-diphtherial corynebacteria are Gram-positive rods that cause opportunistic infections, what is supported by their ability to produce biofilm on artificial surfaces. In this study, the characteristic of the biofilm produced on vascular and urological catheters was determined using a confocal microscopy for the most frequently involved in infections diphtheroid species. They were represented by the reference strains of Corynebacterium striatum ATCC 6940 and C. amycolatum ATCC 700207
ALINA OLENDER,
AGNIESZKA BOGUT,
AGNIESZKA MAGRYŚ,
KATARZYNA KRÓL-TURMIŃSKA
Polish Journal of Microbiology , ISSUE 4, 431–440
original-paper
of 142 patients was recorded (Linnan et al. 1988). Another listeriosis outbreaks linked to the contaminated cheese took place in Germany in 2006–2007 (Koch et al. 2010), and in Spain in 2008–2009 (Jackson et al. 2011) where 189 and 8 cases were reported, respectively.
Despite the improvement in the hygiene of the production process, L. monocytogenes poses still a serious problem in the food processing plants, including dairies. Such environment favors biofilm formation and bacterial survival
KRZYSZTOF SKOWRON,
EWA WAŁECKA-ZACHARKSA,
KATARZYNA GRUDLEWSKA,
NATALIA WIKTORCZYK,
AGNIESZKA KACZMAREK,
GRZEGORZ GRYŃ,
JOANNA KWIECIŃSKA-PIRÓG,
KLAUDIA JUSZCZUK,
ZBIGNIEW PALUSZAK,
KATARZYNA KOSEK-PASZKOWSKA,
EUGENIA GOSPODAREK-KOMKOWSKA
Polish Journal of Microbiology , ISSUE 3, 353–369
Short Communication
Transcriptome changes of biofilm-forming Staphylococcus epidermidis response to total alkaloids of Sophorea alopecuroides was observed. Bioinformatic analyses were further used to compare the differential gene expression between control and the treated samples. It was found that 282 genes were differentially expressed, with 92 up-regulated and 190 down-regulated. These involved down-regulation of the sulfur metabolism pathway. It was suggested that inhibitory effects on Staphylococcus
CUI-PING GUAN,
HUI-XIA LUO,
H.E. FANG,
XUE-ZHANG ZHOU
Polish Journal of Microbiology , ISSUE 2, 223–226
Research Article
-associated infections. Staphylococcus epidermidis is mainly responsible for bloodstream infections and other biomedical device-related infections. Hospital strains of Staphylococcus epidermidis form protective biofilm and are characterized by their resistance to various antibiotics.
Beata Podgórska,
Danuta Kędzia
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 4, 338–348
Article
, virulence, symbiosis or biofilm formation. In combination with GTP, this protein is able to bind to the 70S bacterial ribosome at the same site as where it interacts with Ef-G, Ef-Tu and Ef4. As a result of this interaction, BipA is able to regulate the translation process, in particular with regard to proteins involved in the response of bacteria to stress. The involvement of the BipA protein in the biogenesis of ribosomal subunits and assembling the 70S monosome is evidenced by the fact that the bipA
Julia Berdychowska,
Justyna Boniecka,
Grażyna B. Dąbrowska
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 127–142
Original Paper
Badreddine Mechri,
Amel Medhioub,
Mohamed Nejib Medhioub,
Mahjoub Aouni
Polish Journal of Microbiology , ISSUE 3, 307–318
Research Article
during stressful conditions, among others. H. pylori OMV mediate transfer of virulence factors such as toxins and immunomodulatory compounds. They contribute to avoiding a response from the host immune system and inducing chronic gastritis. OMV secretion also affects the formation of cell aggregates, microcolonies and biofilm matrix. Enhanced OMV production is connected to maintenance of direct contact through cell-cell and cell-surface interactions. A key component of OMV, which determines their
Paweł Krzyżek
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 316–325
original-paper
(Mathur et al. 2018). Cramton and coworkers reported that SA was more frequently isolated from corneal infections related to the contact lenses wearing (Cramton et al. 1999). The extended wear of contact lenses and lack of eye hygiene increase the risk of keratitis. The morbidity of ocular infections is associated with the increasing number of cataract surgery and lens replacement (Astley et al. 2019). The ability of SA strains to aggregate and form biofilm is related to their capacity of producing
MARTA KŁOS,
MONIKA POMORSKA-WESOŁOWSKA,
DOROTA ROMANISZYN,
AGNIESZKA CHMIELARCZYK,
JADWIGA WÓJKOWSKA-MACH
Polish Journal of Microbiology , ISSUE 4, 541–548
Research Article
belonging to ST2, the most frequently found clonal type of hospital-associated invasive S. epidermidis, are characterized by bacterial biofilm formation and resistance to methicillin, amongst other antibiotics. S. epidermidis is mainly responsible for bloodstream infections and other biomedical device-related infections. Treating infections characterized by biofilm formation is problematic. An additional challenge is differentiation between actual S. epidermidis bloodstream infections and blood probe
Beata Podgórska,
Danuta Kędzia
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 4, 348–359
Research Article
Barbara Katarzyna Pawłowska,
Beata Magdalena Sobieszczańska
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 77–87
original-paper
Introduction
The role of oral health, including the level of dental hygiene, accumulation of dental bacterial plaque, and periodontal diseases in the pathogenesis of respiratory infections is usually ignored in the medical care of cystic fibrosis (CF) patients. The oral cavity is a reservoir of many microorganisms; to date, more than 700 oral bacterial species have been identified. Over 400 of them form dental bacterial plaque biofilm and can be isolated from gingival sulcus or gingival pocket
TAMARA PAWLACZYK-KAMIEŃSKA,
RENATA ŚNIATAŁA,
HALINA BATURA-GABRYEL,
MARIA BORYSEWICZ-LEWICKA,
SZCZEPAN COFTA
Polish Journal of Microbiology , ISSUE 3, 377–382
Short Communication
, none of these strains showed resistance to tigecycline and only four strains were resistant to rifampin i.e. antibiotics which are very efficient in treating biofilm-associated infections.
Ewa Szczuka,
Karolina Bosacka,
Adam Kaznowski
Polish Journal of Microbiology , ISSUE 2, 215–217
Short Communication
From 123 clinical and environmental Pseudomonas aeruginosa isolates, 24 strains were selected for their similar antibioresistance, virulence and biofilm formation profiles, to examine their diversity and occurrence of clones within two hospitals and different natural sites in Meknes (Morocco). Pulsed-field gel electrophoresis, using DraI enzyme, didn’t reveal a close relationship between clinical and environmentalisolates nor between strains of the two hospitals. 19 genotypes were
Itto Maroui,
Abouddihaj Barguigua,
Asmae Aboulkacem,
Hanane Elhafa,
Khadija Ouarrak,
Mohammed Sbiti,
Lhoussain Louzi,
Mohammed Timinouni,
Abdelhaq Belhaj
Polish Journal of Microbiology , ISSUE 3, 397–400
original-paper
Introduction
Periodontitis is a polymicrobial biofilm-mediated disease of the oral cavity and is a chronic inflammatory condition of the periodontium, leading to the damage of structural tooth support, resorption of bone, and eventually tooth loss (Schaudinn et al. 2009). The microbial etiology of periodontitis is defined by oral biofilm, also called dental plaque, in which resides an interdependent microbial community containing numerous bacterial species (Sundus et al. 2016). A typical dental
JI-HOI MOON,
MI HEE NOH,
EUN-YOUNG JANG,
SEOK BIN YANG,
SANG WOOK KANG,
KYU HWAN KWACK,
JAE-IN RYU,
JIN-YONG LEE
Polish Journal of Microbiology , ISSUE 2, 263–268
minireview
environment enriched with nutrient germinants (L-valine) and non-nutrient germinants (dodecylamine, Ca2+DPA). Their results indicated the potential inactivation effects of the plasma on germination receptors but the germination induced by L-valine was inhibited.
Bacterial biofilm. Many microorganisms live in the environment as biofilms rather than free-living organisms. Biofilm was defined as the cells adhering to a solid surface and surrounded by an extracellular matrix produced by them (Czapka et al
IWONA NIEDŹWIEDŹ,
ADAM WAŚKO,
JOANNA PAWŁAT,
MAGDALENA POLAK-BERECKA
Polish Journal of Microbiology , ISSUE 2, 153–164
Original Paper
Joanna Stefańska,
Karolina Stępień,
Anna Bielenica,
Małgorzata Wrzosek,
Marta Struga
Polish Journal of Microbiology , ISSUE 4, 451–460
Short Communication
Witold Woźniak,
Aleksandra Kozińska,
Piotr Ciostek,
Izabela Sitkiewicz
Polish Journal of Microbiology , ISSUE 1, 125–129
Research Article
archaea in pipelines hinders the correct operation of the network, through the accumulation of biofilm and reduction in the pipe diameter.
Agnieszka Staniszewska,
Alin Kunicka-Styczyńska,
Krzysztof Ziemiński
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 4, 381–388
research-article
equipment to the network [5–7].
The main objective of the paper is to present a methodology for analysing and assessing the risk of biological stability in terms of the water consumers safety.
2.
RESEARCH METHODOLOGY
2.1.
Water biostability
In water distribution systems more than 95% of the microorganisms is on the internal surfaces of water pipes in the form of biofilm. Biofilm is a group of microorganisms that are linked by trophic dependence from autotrophic and heterotrophic bacteria and fungal
Barbara TCHÓRZEWSKA-CIEŚLAK,
Dorota PAPCIAK,
Katarzyna PIETRUCHA-URBANIK,
Andżelika PIETRZYK
Architecture, Civil Engineering, Environment , ISSUE 1, 149–154
Original Paper
%, respectively) and sessile form hosted in dentinal tubules (to 19%, 13% and 52%, respectively), relative to the controls. S. salivarius was the least affected in planktonic (94% viability) and sessile form (86% viability). In conclusion, sensitivity to photosensitization is species-dependent and sessile biofilm cells are affected to the same extent as their planktonic counterparts.
HOMA DARMANI,
KHITAM H. TAWALBEH,
AHMAD S. AL-HIYASAT,
MOHAMMAD-ALI AL-AKHRAS
Polish Journal of Microbiology , ISSUE 4, 455–462
Article
żelaza [4, 38]. Ponadto ogromną rolę w patomechanizmie ZUM odgrywa zdolność tworzenia biofilmu przez szczepy UPEC. Biofilm stanowi bowiem skuteczną barierę dla stosowanych antybiotyków, co niejednokrotnie prowadzi do niepowodzeń terapeutycznych oraz przyczynia się do przewlekłych i nawracających stanów zapalnych układu moczowego [41].
Geny kodujące czynniki wirulencji szczepów UPEC umiejscowione są na ruchomych elementach genetycznych przenoszonych drogą horyzontalnego transferu genów (horizontal
Sylwia Joanna Chmielewska,
Katarzyna Leszczyńska
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 25–37