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Research Article

THE APPLICATION OF GENOTYPING AND PHENOTYPING TECHNIQUES FOR EPIDEMIOLOGICAL ANALYSIS OF MICROORGANISMS

. This review discusses and compares methods facilitating bacterial typing at a strain level. Phenotyping methods analysed in this article are: Biotyping, Antimicrobial Susceptibility Typing, Phage Typing and protein-based methods. Genotyping techniques reviewed in this article are based on digestion of genomic DNA, methods using amplification of DNA, and based on sequencing DNA. This would include Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS). Methods used in identification of

Marcin Brzozowski, Paweł Kwiatkowski, Joanna Jursa-Kulesza, Danuta Kosik-Bogacka

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 353–366

Original Paper

Genetic Analysis Method for Staphylococcus chromogenes Associated with Goat Mastitis

Mastitis in goats is mainly caused by coagulase-negative Staphylococcus (CNS). The identification methods for this group are based on evaluating the expression of phenotypic characteristics such as the ability to metabolize various substrates; however, this is disadvantageous as these methods are dependent on gene expression. In recent years, genotyping methods such as the Multiple Locus Variable-Number Tandem Repeat Analysis (MLVA) and gene identification have been useful for epidemiological

ROCÍO A. RUIZ-ROMERO, ROBERTO A. CERVANTES-OLIVARES, ANDRÉS E. DUCOING-WATTY, DANIEL MARTÍNEZ-GÓMEZ, EFRÉN DÍAZ-APARICIO, ESTELA T. MÉNDEZ-OLVERA

Polish Journal of Microbiology , ISSUE 2, –

Research Article

IMPLEMENTATION OF WHOLE GENOME SEQUENCING FOR BACTERIA GENOTYPING

Daria Artyszuk, Tomasz Wołkowicz

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 179–193

Review

A Caucasian JK*A/JK*B woman with Jk(a+b–) red blood cells, anti-Jkb, and a novel JK*B allele c.1038delG

The Kidd blood group on the red blood cell (RBC) glycoprotein urea transporter-B has a growing number of weak and null alleles in its gene SLC14A1 that are emerging from more widespread genotyping of blood donors and patients. We investigated a 64-year-old Caucasian woman of Polish-Czech descent who developed anti-Jkb detected in solid-phase RBC adherence testing within 12 days after 7 units of RBCs were transfused. Her RBCs subsequently typed Jk(a+b–) by licensed reagents and human

Glenn Ramsey, Ricardo D. Sumugod, Paul F. Lindholm, Jules G. Zinni, Jessica A. Keller, Trina Horn, Margaret A. Keller

Immunohematology , ISSUE 3, 91–95

Original Paper

Modeling alloantibody formation to highincidence red blood cell antigens in immune responders using genotypic data  

Alloimmunization to red blood cell antigens is unpredictable and poorly understood. Patients who are negative for highincidence antigens (HIAs) are at risk for developing the corresponding antibodies. Molecular methods can easily predict the lack of an antigen and thus, the risk of an individual to become immunized. We examined the prevalence and risk factors for HIA alloimmunization in patients at risk based on genotyping results. Genotyping using a molecular method (HEA BeadChip&trade

Patricia A.R. Brunker, Keerthana Ravindran, R. Sue Shirey

Immunohematology , ISSUE 1, 9–14

Article

Assessment of common red blood cell pretreatments to yield an accurate serologic antigen phenotype compared with genotype-predicted phenotype

disease, or microhematocrit centrifugation to isolate reticulocytes—are often used in an attempt to obtain a phenotype.6 The effectiveness of removing IgG from RBCs to obtain DAT-negative RBCs can vary between methods.7 With the increasing availability of RBC genotyping, more blood banks are using this testing to obtain a predicted RBC phenotype as an alternative to RBC pretreatments followed by serologic antigen typing.8 A RBC genotyping panel such as the U.S. Food and Drug Administration (FDA

T. Horn, J. Hamilton, J. Kosanke, V.W. Hare, W. Kluver, W. Beres, S. Nance, M.A. Keller

Immunohematology , ISSUE 4, 147–151

Original Paper

Genotyping and Clinicoepidemiological Characterization of Rotavirus Acute Gastroenteritis in Egyptian Children

Niveen Saudy, Walaa Othman Elshabrawy, Ahmed Megahed, Mona F. Foad, Aly F. Mohamed

Polish Journal of Microbiology , ISSUE 4, 433–442

original-paper

Molecular Diversity of Staphylococcus aureus Colonizing the Upper Respiratory Tract of Residents and Staff in A Nursing Home

further analysis using a medium of tryptic soy broth (TSB; BTL, Poland) and glycerol (POCH, Poland) in equal proportions (1:1). Multiple-locus variable-number tandem-repeat fingerprinting (MLVF). The MLVF genotyping was performed to analyze the genetic diversity and relatedness of all 66 identified S. aureus strains. The MLVF method involved a multiplex-PCR reaction targeting five variable number of tandem repeats (VNTRs) in the following loci: sspA, spa, sdr, clfA, and clfB. These five VNTRs are

MARTYNA KASELA, AGNIESZKA GRZEGORCZYK, ANNA MALM

Polish Journal of Microbiology , ISSUE 3, 371–376

Review

The Vel blood group system: a review

Jill R. Storry, Thierry Peyrard

Immunohematology , ISSUE 2, 56–59

Original Paper

Gas Gangrene of Different Origin Associated with Clostridium perfringens Type A in Three Patients Simultaneously Hospitalized in a Single Department of Orthopedics and Traumatology in Poland

April 2015 and 20th April 2015. The three C. perfrin­gens isolates studied had identical biochemical profiles. Two isolates had identical resistance patterns, while the third presented a different profile. Using the multiplex PCR method, all isolates showed the presence of cpa gene encoding α-toxin; furthermore, the presence of the cpb2 gene encoding β2-toxin was confirmed in two isolates. Genotyping with the use of pulsed field gel electrophoresis (PFGE) indicated that the isolates

Monika Brzychczy-Włoch, Dorota Ochońska, Anna Piotrowska, Małgorzata Bulanda

Polish Journal of Microbiology , ISSUE 4, 399–406

Short Communication

Clonal Analysis of Clinical and Environmental Pseudomonas aeruginosa Isolates from Meknes Region, Morocco

Itto Maroui, Abouddihaj Barguigua, Asmae Aboulkacem, Hanane Elhafa, Khadija Ouarrak, Mohammed Sbiti, Lhoussain Louzi, Mohammed Timinouni, Abdelhaq Belhaj

Polish Journal of Microbiology , ISSUE 3, 397–400

Short Communication

Genetic Characterization of Human Enteroviruses Associated with Hand, Foot and Mouth Diseases in Poland, 2013–2016

Magdalena Wieczorek, Agnieszka Ciąćka, Arleta Krzysztoszek, Agnieszka Figas, Leszek Szenborn

Polish Journal of Microbiology , ISSUE 3, 405–409

Report

Red blood cell phenotype prevalence in blood donors who self-identify as Hispanic

Molecular genotyping platforms provide a quick, high-throughput method for identifying red blood cell units for patients on extended phenotype-matching protocols, such as those with sickle cell disease or thalassemia. Most of the antigen prevalence data reported are for non-Hispanic populations. Therefore, this study sought to determine the phenotype prevalence in a single blood center’s Hispanic population and to compare those results with previously reported rates in non-Hispanic donor

Chelsea A. Sheppard, Nicole L. Bolen, Beth Eades, Gorka Ochoa-Garay, Mark H. Yazer

Immunohematology , ISSUE 3, 119–124

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