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  • Immunohematology

 

Article | 13-April-2020

Problems highlighted when using anticoagulated samples in the standard tube low ionic strength antiglobulin test

Amanda J Sweeney

Immunohematology, Volume 22 , ISSUE 2, 72–77

Article | 16-October-2019

Cold autoadsorption

Cold-reactive autoantibodies can mask the presence of underlying clinically significant alloantibodies in a patient’s plasma or serum. These autoantibodies are problematic when performing laboratory procedures such as ABO typing, red blood cell (RBC) crossmatching, antibody detection testing, and antibody identification. To avert the masking of clinically significant alloantibodies in a patient’s plasma or serum, adsorption studies can be performed at 4°C using autologous RBCs

Ernest M. Ekema

Immunohematology, Volume 34 , ISSUE 4, 158–160

Review | 01-December-2019

Warm autoadsorption with enzyme-treated red blood cells

Patients demonstrating warm autoantibody specificity present serologic challenges for laboratory staff performing antibody identification in the blood bank. Autoantibody can be removed from plasma or serum by adsorption onto autologous red blood cells (RBCs) provided the patient has not been transfused in the previous 3 months. The adsorption process can be enhanced by enzyme pretreatment of autologous RBCs.

Farai Tsimba-Chitsva, Susanne Bishop, Kelly Kezeor

Immunohematology, Volume 28 , ISSUE 3, 88–90

Article | 16-February-2021

Acidification of plasma for detection of pH-dependent antibodies

antigen–antibody complex.6 Reagents/Supplies Reagents Supplies Plasma or serum containing antibody of interest 0.2 N HCL (1.0 N HCL diluted with distilled H2O) Isotonic saline Reagent RBCs Anti-IgG (optional) IgG–coated RBCs (optional) Test tubes pH meter or pH strips Transfer pipettes Centrifuge Cell washer Agglutination viewer Microscope 37°C incubator or water bath 4°C refrigerator or ice bath HCL = hydrochloric acid; RBCs = red blood cells. Procedural Steps Mix

K.L. Bowman, B.C. Dunlap, L.M. Hawthorne, K.L. Billingsley

Immunohematology, Volume 35 , ISSUE 3, 116–118

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