In the nematode family Criconematidae, a taxonomy primarily based on cuticle characters has created classifications that are notoriously volatile. Molecular characters may lead to their stabilization. A phylogenetic tree of Criconematoidea was constructed using 166 new near full-length 18S rDNA sequences and 58 sequences from GenBank. Bayesian and maximum likelihood (ML) analyses produced trees with similar topologies. Major features include a strongly supported clade that includes
Journal of Nematology , ISSUE 3, 236–244
duct, offset spermatheca filled with small spheroid sperm cells, 106 to 127 µm long elongate-conoid tail with filiform distal region and finely rounded tip. Molecular phylogenetic analyses were performed using a near-full length fragment of the 18S rDNA and the D2–D3 expansion segments of the 28S rDNA using Bayesian inference and maximum likelihood methods. In the inferred phylogenetic tree with 18S rDNA, the new species has a close affinity with several isolates of the type species, Labrys
Robin M. Giblin-Davis,
Farahnaz Jahanshahi Afshar,
Journal of Nematology , ISSUE 3, 343–354
molecular analysis of many D. weischeri specimens from Canada is presented. Individuals from 41C. arvense or yellow pea grain samples with seeds of C. arvense from the Prairie Provinces were sequenced for the internal transcribed spacer (ITS rDNA), large subunit (LSU) D2D3 28S rDNA, partial segment of small subunit (SSU) 18S rDNA, and the heat shock protein Hsp90 gene. The analysis also included D. weischeri individuals from C. arvense from Russia and garlic with D. dipsaci from the Provinces of Ontario
Journal of Nematology , ISSUE 2, 163–182
SERGIO A LVAREZ-ORTEGA,
MOHAMMAD REZA ATIGHI,
Journal of Nematology , ISSUE 3, 214–221
) according to manufacturer’s instructions. Plasmid clone DNA was prepared with the QiaPrep Spin Miniprep Kit (Qiagen, Valencia, CA) and digested with Eco RI to verify the presence of insert. Sequencing was performed by Genewiz, Inc. Direct sequencing of PCR amplicons was used to obtain the 28S sequences (assigned GenBank accession numbers MK121965-MK121968), the ITS 1&2 rDNA sequence (MK121952), the 18S rDNA sequence (MK182465), and COI sequences (MK253554-MK253558). Accession numbers were assigned for
Andrea M. Skantar,
Zafar A. Handoo,
Mihail R. Kantor,
Lynn K. Carta,
Journal of Nematology , 1–8
sequences appeared separated into two clades.
Bayesian inference tree showing the phylogenetic position of Eucephalobus and related taxa based on sequences of the 18S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.
Bayesian Inference tree showing the phylogenetic position of Eucephalobus and related taxa based on sequences of the 28S rDNA region. Bayesian posterior probabilities (%) are given for
Journal of Nematology , 1–20
, the mixture was incubated at room temperature (23 ± 2°C) for 10 min and then at 95°C for 3 min. Finally, 20 µL of neutralization solution was added to the tube and vortexed briefly. This DNA extract was stored at −20°C and used as DNA template for PCR reactions in 2 µL aliquots. Three primer pairs targeting 18S rDNA (360F/932R), 28S rDNA (D2A/D3B) and ITS1 rDNA (BL18/5818) (Riga et al., 2007; Duarte et al., 2010; Ye et al., 2015) were used in singleplex PCR (Hajihassani et al., 2018a). The
Ganpati B. Jagdale,
Alfredo Dick Martinez-Espinoza
Journal of Nematology , 1–3
of hermaphroditism in this species.
One 923 bp 18S rDNA sequence (GenBank accession number MK959600) and three identical 637 bp without changes or deletions each other 28S rDNA sequences (GenBank accession numbers MK959601-MK959603) were obtained from three specimens. Both trees show O. dolichura as the sister species of O. saproxylicus sp. n. With respect to O. dolichura, the 18S fragment (with 914 bp), show four changes or deletions while the 28S fragments (with 637
journal of nematology , 1–21
based on sequences of D2-D3 expansion region of 28S and 18S rDNA, confirmed its status as a new species.
Journal of Nematology , ISSUE 1, 67–76
Juvenile, female and male nematodes were discovered in wood chips of white pine Pinus strobus from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics. The specimens were identified as Bursaphelenchus antoniae Penas et al., 2006 based on 18S rDNA molecular sequence vs
Lynn K. Carta,
R. L. Wick
Journal of Nematology , ISSUE 4, 473–478
: 9.94% in Ischnura fluviatilis and 4.76% in Rhionaeschna bonaerensis.
Specimens deposited: all types have been deposited in the Colección Helmintológica del Museo de Ciencias Naturales de La Plata with the following accession numbers: Holotype male No. MLP-He 7639, allotype female No. MLP-He 7640, and one paratype (postparasitic juvenile) No. MLP-He 7641.
Etymology: this species is named after Enzo Rusconi, nephew of JMR.
DNA characterization and phylogenetic analysis
The 18S rDNA sequence of
José Matias Rusconi,
Cristian Di Battista,
María Fernanda Achinelly
Journal of Nematology , 1–9
, joins together anterior to the spicules, and is partially extended and decorated with microvilli. The spicules are incompletely separated, and the tail does not extend beyond the bursa. Phylogenetic trees of 18S rDNA and internal transcribed spacer indicate that the new species belongs to the insectivora group of the genus Oscheius; it is most closely related to O. myriophilus, and the two species can be distinguished on the basis of their different body length, morphological
Journal of Nematology , ISSUE 1, 33–41
from those of other closely related species of Acrobeloides by 9 to 89 bp (Table 5). The D2-D3 segment of 28 S rDNA gene in the Indian isolates differed in 5 to 76 bp from other closely related species of Acrobeloides (Table 6).
Pairwise distances of the 18 S rDNA regions between present strains of Acrobeloides and already described species.
MK935150 A. saeedi KMW
Aashaq Hussain Bhat,
Ashok Kumar Chaubey,
Journal of Nematology , 1–21