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  • Journal Of Nematology

 

research-article

On the synonymy of Trophotylenchulus asoensis and T. okamotoi with T. arenarius, and intra-generic structure of Paratylenchus (Nematoda: Tylenchulidae)

in one clade in the inferred trees of 28S rRNA and ITS gene sequences. The present study provides morphological and molecular support on the synonymy of T. asoensis (Minagawa, 1983; Siddiqi, 1999) and T. okamotoi (Minagawa, 1983; Siddiqi, 1999) with T. arenarius (Raski, 1956; Siddiqi, 1999) based on the observations made on two populations from Iran. Moreover, morphological and molecular characterization of 10 species of Paratylenchus (Micoletzky, 1922) was used to obtain a better insight into

Hossein Mirbabaei, Ali Eskandari, Reza Ghaderi, Akbar Karegar

Journal of Nematology, Volume 51 , 1–14

research-article

On the molecular identity of Paratylenchus nanus Cobb, 1923 (Nematoda: Tylenchida)

analysis DNA was extracted from several specimens using the proteinase K protocol. DNA extraction and PCR protocols were as described by Tanha Maafi et al. (2003). The primer sets D2A (5’ – ACA AGT ACC GTG AGG GAA AGT TG – 3’) and D3B (5’ – TCG GAA GGA ACC AGC TAC TA – 3’) amplifying the D2-D3 of 28S rRNA gene (Subbotin et al., 2006), TW81 (5’ – GTT TCC GTA GGT GAA CCT GC – 3’) and AB28 (5’ – ATA TGC TTA AGT TCA GCG GGT – 3’) amplifying ITS rRNA (Tanha Maafi et al., 2003) and COIF5 (5’ – AAT WTW GGT

Sergei A. Subbotin, Guiping Yan, Mihail Kantor, Zafar Handoo

Journal of Nematology, Volume 52 , 1–7

research-article

First report of a stunt nematode Tylenchorhynchus zeae on corn in Gansu Province, China

the Proteinase K method (Kumari and Subbotin, 2012), and the internal transcribed spacer1 (ITS1) region of rDNA and D2/D3 fragments of the 28S rRNA were amplified with universal primers rDNA2 and rDNA1.58 s (Szalanski et al., 1997), D2A and D3B (Castillo et al., 2003), respectively. PCR products were sequenced by Tsingke Biological Technology (Beijing, China). The ITS1 sequence (MN757910, 721 bp) and D2/D3 sequence (MN757911, 802 bp) were submitted to GenBank and compared with published sequences

Zhi Peng Xu, Hui Xia Li, Yong Gang Liu, Bao Cang Ren, Chun Hui Ni, Jin Hui Ma

Journal of Nematology, Volume 52 , 1–2

research-article

A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

shape parameters and substitution rates in the AIC were then given to MrBayes for the phylogenetic analyses. An unlinked general time-reversible model with invariable sites and a gamma-shaped distribution (GTR fo li + G) was used for the D2-D3 expansion segments of 28S rRNA, ITS, partial 18 S, and partial coxI. These BI analyses were run separately per dataset using four chains for 2 × 106 generations for all of the molecular markers. A combined analysis of the three genes was not undertaken due to

Munawar Maria, Wentao Miao, Ruihang Cai, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 52 , 1–14

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