original-paper | 05-August-2020
identified and characterized genotypically and phenotypically.
DNA extraction, rep-PCR, and RFLP-PCR of the 5.8S-ITS region. DNA extraction was performed as per Querol et al. (1992) with a slight modification in the use of lyticase (3.3 U · µ/l; Sigma, USA) instead of zymolase. For discrimination at the strain level, PCR of the repetitive extragenic palindromic sequences (rep-PCR) (Versalovic et al. 1991) was performed using a primer (GTG)5 (5’-GTG GTG GTG GTG GTG-3’) as described by Gori et al. (2013
AMPARO I. ZAVALETA,
PAMELA E. CANALES,
Polish Journal of Microbiology, Volume 69 , ISSUE 3, 251–261