Original Paper
Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of
Rambha K. Shah,
Zhao H. Ni,
Xiao Y. Sun,
Guo Q. Wang,
Fan Li
Polish Journal of Microbiology , ISSUE 4, 501–508
original-paper
6
0.42
3
0.23
3
0.27
47
0.24
Others
65
1.91
236
8.48
199
13.92
47
3.68
43
3.88
590
3.06
During the study period, the detection rate of K. pneumoniae isolates was stable, meanwhile, the rate of extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae (ESBL-K. pneumoniae) showed a downward trend (χ2 = – 4.6619, p < 0.0001). A significant increase of resistance was observed for cefotaxime, meropenem, and imipenem to K. pneumoniae and ESBL-K. pneumoniae. But a significant decrease of
TINGTING MAO,
HUIJUAN ZHAI,
GUANGCAI DUAN,
HAIYAN YANG
Polish Journal of Microbiology , ISSUE 2, 225–232
Short Communication
Four Klebsiella pneumoniae isolates from children hospitalized over 10 months in an intensive care unit in a children’s teaching hospital in Poland were analyzed. All of the isolates belonged to a single pulsotype and sequence type (ST) 11, and produced the KPC-2 carbapenemase and extended-spectrum β-lactamase (ESBL) CTX-M-15. They were resistant to a variety of antimicrobials, and their β-lactam resistance patterns were typical for KPC producers. This is one of few cases of
Monika Machulska,
Anna Baraniak,
Iwona Żak,
Katarzyna Bojarska,
Dorota Żabicka,
Iwona Sowa-Sierant,
Waleria Hryniewicz,
Marek Gniadkowski
Polish Journal of Microbiology , ISSUE 3, 401–404
Short Communication
Bayri Erac,
Fethiye Ferda Yilmaz,
Ismail Ozturk,
Sabire Sohret Aydemir,
Mine Hosgor-Limoncu
Polish Journal of Microbiology , ISSUE 4, 529–532
short-communication
-trimoxazole (25 μg), trimethoprim (5 μg), sulfamethoxazole (200 μg), piperacillin (30 μg), piperacillin/tazobactam (30–60 μg), aztreonam (30 μg), imipenem (10 μg), and meropenem (10 μg). The production of extended spectrum β-lactamases (ESBL) was determined in the double-disc synergy test. All antibiotic discs were provided by Oxoid. Amplifications of three genes conferring resistance to sulfonamides (sul1, sul2, sul3), were conducted in a 25-μl volume with PCR buffer with NH4(SO4)2, 0.5 μM of each primer
EDYTA KONECKA,
JOANNA MOKRACKA,
SYLWIA KRZYMIŃSKA,
ADAM KAZNOWSKI
Polish Journal of Microbiology , ISSUE 2, 185–191
Original Paper
(8.0%) can produce extended-spectrum β-lactamases (ESBL). E. coli was highly sensitive to imipenem (100.0%), gentamicin (91.0%), nitrofurantoin (89.4%), amikacin (88.2%), piperacillin/ tazobactam (87.0%) and cephalosporins (79.7–89.5%). Low sensitivity was found in relation to fluoroquinolones (60.3–70.4%). E. coli was least sensitive to ampicillin (30.2%) and amoxicillin/clavulanic acid (49.9%). We observed a significant fall in susceptibility level to piperacillin/tazobactam (68.4% vs. 88.8%; p
MIKOŁAJ MICHNO,
ANTONI SYDOR,
MARTA WAŁASZEK,
WŁADYSŁAW SUŁOWICZ
Polish Journal of Microbiology , ISSUE 4, 517–524
Article
failure of therapy and the spread of resistance. It soon became clear that the resistance of these bacteria is to a large extent determined by the production of β-lactamases, which are often derivatives of TEM-1, TEM-2 or SHV-1, as well as CTX-M, VEB, PER, BES and OXA, characterised by an extended substrate spectrum (extended-spectrum beta-lactamases, ESBL). OXA β-lactamase also has the characteristics of carbapenemases classified as class D, CHDL type (carbapenem-hydrolysing class D β-lactamase) [39
Marian Binek,
Magdalena Kizerwetter-Świda,
Magdalena Rzewuska,
Dorota Chrobak-Chmiel,
Agnieszka Sałamaszyńska-Guz
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 259–270