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Original Paper

The Determination and Correlation of Various Virulence Genes, ESBL, Serum Bactericidal Effect and Biofilm Formation of Clinical Isolated Classical Klebsiella pneumoniae and Hypervirulent Klebsiella pneumoniae from Respiratory Tract Infected Patients

Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of

Rambha K. Shah, Zhao H. Ni, Xiao Y. Sun, Guo Q. Wang, Fan Li

Polish Journal of Microbiology , ISSUE 4, 501–508

original-paper

Patterns of Drug-Resistant Bacteria in a General Hospital, China, 2011–2016

6 0.42 3 0.23 3 0.27 47 0.24 Others 65 1.91 236 8.48 199 13.92 47 3.68 43 3.88 590 3.06 During the study period, the detection rate of K. pneumoniae isolates was stable, meanwhile, the rate of extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae (ESBL-K. pneumoniae) showed a downward trend (χ2 = – 4.6619, p < 0.0001). A significant increase of resistance was observed for cefotaxime, meropenem, and imipenem to K. pneumoniae and ESBL-K. pneumoniae. But a significant decrease of

TINGTING MAO, HUIJUAN ZHAI, GUANGCAI DUAN, HAIYAN YANG

Polish Journal of Microbiology , ISSUE 2, 225–232

Short Communication

KPC-2-producing Klebsiella pneumoniae ST11 in a Children’s Hospital in Poland

Four Klebsiella pneumoniae isolates from children hospitalized over 10 months in an intensive care unit in a children’s teaching hospital in Poland were analyzed. All of the isolates belonged to a single pulsotype and sequence type (ST) 11, and produced the KPC-2 carbapenemase and extended-spectrum β-lactamase (ESBL) CTX-M-15. They were resistant to a variety of antimicrobials, and their β-lactam resistance patterns were typical for KPC producers. This is one of few cases of

Monika Machulska, Anna Baraniak, Iwona Żak, Katarzyna Bojarska, Dorota Żabicka, Iwona Sowa-Sierant, Waleria Hryniewicz, Marek Gniadkowski

Polish Journal of Microbiology , ISSUE 3, 401–404

Short Communication

Analyses of Plasmids Harbouring Quinolone Resistance Determinants in Enterobacteriaceae Members

Bayri Erac, Fethiye Ferda Yilmaz, Ismail Ozturk, Sabire Sohret Aydemir, Mine Hosgor-Limoncu

Polish Journal of Microbiology , ISSUE 4, 529–532

short-communication

Evaluation of The Pathogenic Potential of Insecticidal Serratia marcescens Strains to Humans

-trimoxazole (25 μg), trimethoprim (5 μg), sulfamethoxazole (200 μg), piperacillin (30 μg), piperacillin/tazobactam (30–60 μg), aztreonam (30 μg), imipenem (10 μg), and meropenem (10 μg). The production of extended spectrum β-lactamases (ESBL) was determined in the double-disc synergy test. All antibiotic discs were provided by Oxoid. Amplifications of three genes conferring resistance to sulfonamides (sul1, sul2, sul3), were conducted in a 25-μl volume with PCR buffer with NH4(SO4)2, 0.5 μM of each primer

EDYTA KONECKA, JOANNA MOKRACKA, SYLWIA KRZYMIŃSKA, ADAM KAZNOWSKI

Polish Journal of Microbiology , ISSUE 2, 185–191

Original Paper

Microbiology and Drug Resistance of Pathogens in Patients Hospitalized at the Nephrology Department in the South of Poland

(8.0%) can produce extended-spectrum β-lactamases (ESBL). E. coli was highly sensitive to imipenem (100.0%), gentamicin (91.0%), nitrofurantoin (89.4%), amikacin (88.2%), piperacillin/ tazobactam (87.0%) and cephalosporins (79.7–89.5%). Low sensitivity was found in relation to fluoroquinolones (60.3–70.4%). E. coli was least sensitive to ampicillin (30.2%) and amoxicillin/clavulanic acid (49.9%). We observed a significant fall in susceptibility level to piperacillin/tazobactam (68.4% vs. 88.8%; p

MIKOŁAJ MICHNO, ANTONI SYDOR, MARTA WAŁASZEK, WŁADYSŁAW SUŁOWICZ

Polish Journal of Microbiology , ISSUE 4, 517–524

Article

ANTIBIOTIC RESISTANCE OF BACTERIA A GROWING THREAT FOR ANIMALS AND PUBLIC HEALTH

failure of therapy and the spread of resistance. It soon became clear that the resistance of these bacteria is to a large extent determined by the production of β-lactamases, which are often derivatives of TEM-1, TEM-2 or SHV-1, as well as CTX-M, VEB, PER, BES and OXA, characterised by an extended substrate spectrum (extended-spectrum beta-lactamases, ESBL). OXA β-lactamase also has the characteristics of carbapenemases classified as class D, CHDL type (carbapenem-hydrolysing class D β-lactamase) [39

Marian Binek, Magdalena Kizerwetter-Świda, Magdalena Rzewuska, Dorota Chrobak-Chmiel, Agnieszka Sałamaszyńska-Guz

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 259–270

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