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mini-review

Antibiotic Resistance Among Uropathogenic Escherichia coli

recommended for the treatment of UTIs. The resistance to antibiotics of UPEC isolated in different regions of the world (European countries, North America, Asia, and some countries of Africa) was compared. The increase of resistance to fluoroquinolones, which was significantly higher in developing countries than in developed countries, has been revealed. Also, the resistance mechanisms identified in these pathogens were discussed. Escherichia coli as a main etiological agent of UTIs The bacteria

BARBARA KOT

Polish Journal of Microbiology , ISSUE 4, 403–415

Original Paper

Expression of the Fluoroquinolones Efflux Pump Genes acrA and mdfA in Urinary Escherichia coli Isolates

Escherichia coli is one of the most frequent causes of urinary tract infections. Efflux system overexpression is reported to contribute to E. coli resistance to several antibiotics. Our aim in this study was to investigate the relation between antibiotic resistance and the expres­sion of the efflux pump genes acrA and mdfA in E. coli by real-time reverse transcription-PCR. We tested the in vitro susceptibilities to 12 antibiotics in 28 clinical isolates of E. coli obtained from urine

Sarah M. Abdelhamid, Rania R. Abozahra

Polish Journal of Microbiology , ISSUE 1, 25–30

Original Paper

Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland

Shiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorrhagic colitis (HC) and hemolytic – uremic syndrome (HUS) worldwide. The ability of STEC strains to cause disease is associated with the presence of wide range of identified and putativevirulence factors including those encoding Shiga toxin. In this study, we examined the

Aleksandra Januszkiewicz, Waldemar Rastawicki

Polish Journal of Microbiology , ISSUE 3, 261–269

short-communication

Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52

AFNAN A. AL-MNASER, MARTIN J. WOODWARD

Polish Journal of Microbiology , ISSUE 1, 121–125

research-article

THE USAGE OF ULTRASOUNDS TO DISINTEGRATE ESCHERICHIA COLI BACTERIA CONTAINED IN TREATED WASTEWATER

, and parasitic protists (protozoa) and worms react in a different way to the disinfection process. Classical disinfection of sewage and by-products that arise during purification processes can be carried out by physical and chemical methods [10]. One of these methods is ultrasonic disintegration. 3. METHODOLOGY OF LABORATORY TESTS The aim of the experiments was to determine the effect of low-frequency ultra-sound (20 and 40 kHz) on the survival of Escherichia coli bacteria present in ultrapure

Eliza HAWRYLIK

Architecture, Civil Engineering, Environment , ISSUE 3, 131–136

Research Article

Detection of Pathogenic Bacteria in Aqueous Media: Assessing the Potential of Real-Time Electromagnetic Wave Sensing

This paper reports on the capabilities of a novel electromagnetic wave sensing method to detect and identify the presence of various pathogenic bacteria in aqueous media. In particular, the change in the electromagnetic wave signal in microwave frequency range is used as an indicator of bacteria presence. The assessment was conducted by recording reflected signal spectra when the sensor was in contact with deionised water, Escherichia coli, sterile nutrient broth and Pseudomonas aeruginosa

I. Nakouti, O. Korostynska, A. Mason, A. I. Al-Shamma’a

International Journal on Smart Sensing and Intelligent Systems , ISSUE 5, 1–6

Article

CHARACTERIZATION OF A NEEDLE-TYPE GIANT MAGNETORESISTANCE SENSOR FOR DETECTION OF ESCHERICHIA COLI’S MAGNETIC MARKER

, designed for the identification and quantification of Escherichia coli O157:H7 bacteria covered by superparamagnetic beads, Dynabeads® MAX E.coli O157. The sensor characteristics, measurement system setup and the properties of the magnetic marker solution are discussed in detail.

Hamidreza Shirzadfar, Mustapha Nadi, Djilali Kourtiche, Sotoshi Yamada, Payman Shahabi

International Journal on Smart Sensing and Intelligent Systems , ISSUE 1, 220–234

Mini Review

Non-antibiotics, Efflux Pumps and Drug Resistance of Gram-negative Rods

observed. It has also been revealed that depending on the concentration, salicylate induces expression of multi-drug resistance efflux pumps in Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Burkholderia cenocepacia. However, salicylate does not affect the expression of the resistance-nodulation-division efflux systems in Stenotrophomonas maltophilia and Acinetobacter baumannii. Most importantly, there were no effects of medicinal products containing some non-antibiotic

AGNIESZKA EWA LAUDY

Polish Journal of Microbiology , ISSUE 2, 129–135

Original Paper

Microbiology and Drug Resistance of Pathogens in Patients Hospitalized at the Nephrology Department in the South of Poland

Abstract A retrospective study was conducted among 498 patients with urinary tract infections (UTI) referred to our department from January 2013 to December 2015. This study was performed to evaluate the etiology of UTI and the antibiotic susceptibility profile of Escherichia coli (E. coli) as the main etiological factor in different age groups. Urine samples were examined using standard microbiological methods. Three hundred sixty-three samples (72.9%) were identified as E. coli, of which 29

MIKOŁAJ MICHNO, ANTONI SYDOR, MARTA WAŁASZEK, WŁADYSŁAW SUŁOWICZ

Polish Journal of Microbiology , ISSUE 4, 517–524

Original Paper

Improved Production of Recombinant Human β-NGF in Escherichia coli – a Bioreactor Scale Study

Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in Escherichia coli in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further

ZAHRA HAJIHASSAN, POURIA GHOLAMI TILKO, SEYEDEH MAHDIEH SADAT

Polish Journal of Microbiology , ISSUE 3, 355–363

Article

THE ROLE AND IMPORTANCE OF SELECTED VIRULENCE FACTORS DETERMINING THE PATHOGENICITY OF UROPATHOGENIC ESCHERICHIA COLI STRAINS

1. Wstęp Pozajelitowe patogenne szczepy Escherichia coli ExPEC (extraintestinal patogenic E. coli) charakteryzują się różnorodnymi genotypami, jak i fenotypami, a w ich obrębie wyróżnia się, m.in. grupę uropatogennych szczepów Escherichia coli (uropathogenic E. coli, UPEC) odpowiedzialnych za 75–95% przypadków zakażeń układu moczowego (ZUM), zarówno u dzieci, jak i osób dorosłych [4, 6, 10, 33, 34]. UPEC mogą kolonizować nabłonek układu moczowego człowieka, lecz o ich chorobotwórczości i

Sylwia Joanna Chmielewska, Katarzyna Leszczyńska

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 25–37

Original Paper

Genetic Characterization of a Novel Composite Transposon Carrying armA and aac(6)-Ib Genes in an Escherichia coli Isolate from Egypt

surrounding the armA gene. Out of 123 isolates, 66 (53.66%) were resistant to at least oneaminoglycoside antibiotic. Only one Escherichia coli isolate (E9ECMO) which was totally resistant to all tested aminoglycosides, was confirmed to have the armA gene in association with blaTEM-1, blaCTX-M-15, blaCTX-M-14 and aac(6)-Ib genes. The armA gene was found to be carried on a large A/C plasmid. Genetic mapping of the armA surrounding region revealed, for the first time, the association of armA with aac(6)-Ib

Mona T. Kashef, Omneya M. Helmy

Polish Journal of Microbiology , ISSUE 2, 163–169

Article

DIFFUSELY ADHERING ESCHERICHIA COLI

1. Introduction Escherichia coli is a facultative anaerobe that is part of the human colon’s microbiota. This organism colonizes the intestines of newborns as early as in the first hours of their life. Most E. coli rods are harmless and its presence is limited to the digestive tract. Nevertheless, in weakened people, patients suffering from immunosuppression and as a result of damage to the intestinal epithelial layer, even these non-pathogenic strains of E. coli may cause extraintestinal

Michał Turniak, Beata Sobieszczańska

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 143–152

Article

THE ROLE OF THE STRINGENT COMPONENTS IN THE REGULATION OF VIRULENCE

dokładnie dla modelowych bakterii, Gram-ujemnych i Gram-dodatnich. U Escherichia coli poziom cztero- i pięciofosforanu guanozyny kontrolowany jest przez aktywność produktów genów relA i spoT. ppGpp i pppGpp mogą być syntetyzowane przez białka RelA i SpoT, a powstają odpowiednio z GDP lub GTP oraz ATP. Z kolei dwufunkcyjne SpoT ma również zdolność hydrolizy ppGpp do GDP i pirofosforanu (PPi) oraz pppGpp do GTP i PPi. Większość Gammaproteobacteria i część Betaproteobacteria, takich jak E. coli, Salmonella

Klaudyna Krause, Klaudia Milewska, Agnieszka Szalewska-Pałasz

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 247–258

Short Communication

Vital Staining of Bacteria by Sunset Yellow Pigment

In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture

Peng Gao, Chengming Sun, Yuxia Li, Xiaohua Zou, Xun Wu, Yan Ling, Caifu Luan, Huipeng Chen

Polish Journal of Microbiology , ISSUE 1, 113–117

short-communication

Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation

ASHVEEN BAINS, JAMES W. WILSON

Polish Journal of Microbiology , ISSUE 4, 559–563

Short Communication

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable suscep­tibility of S. aureus and E. faecalis to treatment while, under the same conditions, E

Moreno Bondi, Anna Mazzini, Simona De Niederhäusern, Ramona Iseppi, Patrizia Messi

Polish Journal of Microbiology , ISSUE 4, 533–536

Original Paper

Characterisation of Antimicrobial Properties of Extracts of Selected Medicinal Plants

antimicrobial activity against the tested strains was demonstrated by black elder (Sambucus nigra L.), black locust (Robinia pseudoacacia L.) and lingonberry (Vaccinium vitis-idaea L.) extracts. The study showed the diverse morphological activity of specific parts of elderberry and quince, which is the effect of different polyphenolic profile of these plants. The yeast Saccharomyces cerevisiae, Escherichia coli, Pseudomonas putida and Bacillus subtilis showed the highest sensitivity to the effect of

Monika Cioch, Paweł Satora, Magdalena Skotniczy, Dorota Semik-Szczurak, Tomasz Tarko

Polish Journal of Microbiology , ISSUE 4, 463–472

Research Article

STRATEGIES FOR THE ANALYSIS OF THIOLOXIDORDUCTASES

Bacterial Dsb (disulfide bond) enzymes are involved in the oxidative folding of many proteins, through the formation of disulfide bonds between thiol groups of cysteine residues. This process is critical for the correct folding and structural stability of many secreted and membrane proteins. The rapidly expanding number of sequenced bacterial genomes has revealed the enormous diversity among bacterial Dsb systems. While the Escherichia coli oxidative protein folding has been studied in great

Elżbieta Katarzyna Jagusztyn-Krynicka, Anna Marta Banaś, Magdalena Joanna Grzeszczuk

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 326–334

research-article

Active and inactive forms of biotin synthase occur in Heterodera glycines

extracted from the overnight culture using a QIAprep Spin Miniprep Kit (Qiagen) and the resulting plasmids were sequenced for verification at the University of Illinois Roy J. Carver Biotechnology Center. Complementation of ΔbioB Escherichia coli with avirulent and virulent alleles of HgBioB A kanamycin-resistant E. coli vitamin B7 auxotroph (K-12 Keio Collection: JW0758-1) was obtained from the Coli Genetic Stock Center at Yale University and used to prepare electrocompetent cells as described by

Khee Man Kwon, Sadia Bekal, Leslie L. Domier, Kris N. Lambert

Journal of Nematology , 1–12

original-paper

New Look on Antifungal Activity of Silver Nanoparticles (AgNPs)

do not exhibit toxicity for mammalian cells (Milic et al. 2015; Zhang et al. 2016a). However, some cytostatic and anti-cancer activity is reported at these concentrations (AshaRani et al. 2009). Antibacterial activity of AgNPs is rather well documented in the literature, mainly against the following species: Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli (Yoon et al. 2007; Li et al. 2010; Guzman et al. 2012, Kanawaria et al. 2018). Some information

BARBARA ŻAROWSKA, TOMASZ KOŹLECKI, MICHAŁ PIEGZA, KATARZYNA JAROS-KOŹLECKA, MAŁGORZATA ROBAK

Polish Journal of Microbiology , ISSUE 4, 515–525

Original Paper

In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs

The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in

Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, István Bors, András Bónai, Brigitta Bóta, Judit Szabó-Fodor

Polish Journal of Microbiology , ISSUE 2, 245–250

Original Research

Optimization of Mixed Solid-state Fermentation of Soybean Meal by Lactobacillus Species and Clostridium butyricum

Staphylococcus aureus and Escherichia coli. These results together suggest that supplementation of both proteases and probiotics in SSF improves the nutritional value of fermented soybean meal and this is suitable as a protein source in animal feed.

LI-WEN SU, YEONG-HSIANG CHENG, FELIX SHIH-HSIANG HSIAO, JIN-CHENG HAN, YU-HSIANG YU

Polish Journal of Microbiology , ISSUE 3, 297–305

original-paper

Patterns of Drug-Resistant Bacteria in a General Hospital, China, 2011–2016

a more complete picture of bacterial infections and to help clinicians and decision-making departments undertake the proper decisions for patients and antibiotic use. Experimental Materials and Methods Based on the data from a general hospital in Zhengzhou, Henan province, China from 2011 to 2016, five dominant bacteria (Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii) were investigated in this study. The antibiotic

TINGTING MAO, HUIJUAN ZHAI, GUANGCAI DUAN, HAIYAN YANG

Polish Journal of Microbiology , ISSUE 2, 225–232

Research Article

TYPE VB AND VI SECRETION SYSTEMS AS COMPETITION AGENTS OF GRAM-NEGATIVE BACTERIA

phenomenon. A contact-dependent growth inhibition (CDI), primarily found in Escherichia coli strains, utilizes CdiB/CdiA protein of type Vb secretion system, described also as two-partner secretion (TPS) system, to inhibit growth of non-kin strains, where cell contact is required. Presence of an intracellular small immunity protein (CdiI) protects E. coli cells from autoinhibition. Other bacterial competition system, involved mainly in the nodulation process of Rhizobium leguminosarum bv. Trifolii strain

Dawid Gmiter, Grzegorz Czerwonka, Wiesław Kaca

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 4, 360–373

Article

ANTIBIOTIC RESISTANCE OF BACTERIA A GROWING THREAT FOR ANIMALS AND PUBLIC HEALTH

%). Moreover, 55–64% of the isolates demonstrated cross-resistance to ciprofloxacin and nalidixic acid. Table II Percentage of commensal Escherichia coli strains resistant to selected antimicrobials, isolated from fattening pigs Country/average in EU Number of strains tested Percentage of resistant strains Gentamicin Chloramphenicol Ampicillin Cefotaxime Ceftazidime Ciprofloxacin Colistine Sulfamethoxazole Tetracicline Spain 170 4.7 35.3 77.1 2.9 2.9 44.7 0.6 63.5 88.8 Portugal 142

Marian Binek, Magdalena Kizerwetter-Świda, Magdalena Rzewuska, Dorota Chrobak-Chmiel, Agnieszka Sałamaszyńska-Guz

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 259–270

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