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short-communication

Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation

. 2013). In this report, we demonstrate the first-time use of an IncP-1β plasmid (R751) as a gene capture vehicle via the FRT-Capture technique. Recombineering-based approaches such as FRT Capture and other techniques allow the convenient cloning and/or manipulation of large DNA fragments using PCR and associated insertional/recombination steps (Wilson and Nickerson 2006; Narayanan and Chen 2011; Zeng, Zang, et al. 2017; Zeng, Hao, et al. 2017; Graf et al. 2018; Zeng et al. 2018). The development of

ASHVEEN BAINS, JAMES W. WILSON

Polish Journal of Microbiology , ISSUE 4, 559–563

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