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Research Article

The Draft Genome of Globodera ellingtonae

Globodera ellingtonae is a newly described potato cyst nematode (PCN) found in Idaho, Oregon, and Argentina. Here, we present a genome assembly for G. ellingtonae, a relative of the quarantine nematodes G. pallida and G. rostochiensis, produced using data from Illumina and Pacific Biosciences DNA sequencing technologies.

WENDY S. PHILLIPS, DANA K. HOWE, AMANDA M. V. BROWN, SEBASTIAN EVES-VAN DEN AKKER, LEVI DETTWYLER, AMY B. PEETZ, DEE R. DENVER, INGA A. ZASADA

Journal of Nematology , ISSUE 2, 127–128

research-article

The draft genome of Ditylenchus dipsaci

and Brzeski, 1991). In this study, we report the first genome assembly of D. dipsaci, a resource that will improve our understanding of this important parasite. A population of D. dipsaci (E-105) was obtained from the Canadian National Collection of Insects, Arachnids, and Nematodes and originally isolated from garlic in Northern Ontario (Qiao et al., 2013). The nematodes were multiplied in vitro on pea sprout inoculated with only 15 mixed-stages individuals, as described in Poirier et al. (2019

Benjamin Mimee, Etienne Lord, Pierre-Yves Véronneau, Rick Masonbrink, Qing Yu, Sebastian Eves-van den Akker

Journal of Nematology , 1–3

Research Article

GENOME SHUFFLING AS AN ALTERNATIVE METHOD OF IMPROVING THE PROPERTIES OF DISTILLERY YEAST

most promising and effective methods is genome shuffling, allowing the creation of hybrids whose genome is a combination of large DNA fragments derived from strains with distinct phenotypic traits. Genome shuffling creates a chance that the new strain will have valuable functional genes, including their full operons. This, in turn, increases the chance of a long-term maintenance of beneficial technological features by the obtained hybrids.

Aleksandra Wawro

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 278–285

research-article

Genome sequence of the root-knot nematode Meloidogyne luci

parthenogenesis (except M. floridensis) and parasitize a broader range of hosts than their sexual relatives (Castagnone-Sereno and Danchin, 2014). Several genomes of Clade I tropical Meloidogyne spp. have been sequenced (Abad et al., 2008; Lunt et al., 2014; Blanc-Mathieu et al., 2017; Szitenberg et al., 2017) and have revealed them to be complex allopolyploids with heterozygous duplicated genome regions and abundant transposable elements (Blanc-Mathieu et al., 2017; Szitenberg et al., 2017). Previous genome

Nik Susič, Georgios D. Koutsovoulos, Cristian Riccio, Etienne G. J. Danchin, Mark L. Blaxter, David H. Lunt, Polona Strajnar, Saša Širca, Gregor Urek, Barbara Gerič Stare

Journal of Nematology , 1–5

research-article

The Draft Genome of Deladenus siricidicola

effective control of S. noctilio is via the biological control agent, Deladenus siricidicola (Tylenchida: Neotylenchidae). This nematode has a bicyclic life-cycle, where in the free-living phase it reproduces in wood whilst feeding on A. areolatum, while in the parasitic phase it infects S. noctilio larvae and results in sterilized females. The parasitized adult female S. noctilio becomes the natural vector that disperses D. siricidicola into new trees. In this study, the genome of D. siricidicola was

Alisa Postma, X. Osmond Mlonyeni, Frederick Clasen, Fourie Joubert, Bernard Slippers

Journal of Nematology , 1–4

Research Article

Nematode Genome Announcement: A Draft Genome for Rice Root-Knot Nematode, Meloidogyne graminicola

The rice root-knot nematode Meloidogyne graminicola has emerged as a devastating pest of rice in South-East Asian countries. Here we present a draft genome sequence for M. graminicola, assembled using data from short and long insert libraries sequenced on Illumina GAIIx sequencing platform.

Vishal Singh Somvanshi, Madhura Tathode, Rohit Nandan Shukla, Uma Rao

Journal of Nematology , ISSUE 2, 111–116

Research Article

IMPLEMENTATION OF WHOLE GENOME SEQUENCING FOR BACTERIA GENOTYPING

molecular microbiology, thus providing modern tools for the identification of sources and routes of infection. Whole genome sequencing and other high-throughput typing methods are becoming increasingly popular and, thanks to their high resolution, they are ideal tools for comparative analysis of bacteria. This study reviews the methods most commonly used in the molecular typing of bacteria, including those which are in the development stage and may be the main tool in microbial typing in the future.

Daria Artyszuk, Tomasz Wołkowicz

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 179–193

research-article

A draft genome of a field-collected Steinernema feltiae strain NW

The genome encodes the entire inheritance messages of living organisms, serving as the foundation for biological, cellular, and molecular processes that are essential for development and reproduction. Knowledge of genomes advances the understanding of complex gene networks and assists in the engineering of crops and biological control agents for trait improvement (Bolger et al., 2014; Lu et al., 2016; Rodríguez-Leal et al., 2017). Research efforts to date have largely focused on model species

Zhen Fu, Yuxiang Li, Axel A. Elling, William E. Snyder

Journal of Nematology , 1–7

Article

Introduction to Nematode Genome and Transcriptome Announcements in the Journal of Nematology

The Journal of Nematology now offers publication of Nematode Genome Announcements (NGA) and Nematode Transcriptome Announcements (NTA). These brief reports announce the sequencing and assembly of a nematode genome or transcriptome resource, along with basic technical information on DNA sequencing and bioinformatic methods used. This publishing initiative offers a new avenue to openly and concisely communicate the availability and relevance of genome and transcriptome sequence resources to the

DEE R. DENVER, ERIK J. RAGSDALE, W. KELLEY THOMAS, INGA A. ZASADA

Journal of Nematology , ISSUE 2, 125–126

research-article

A draft genome of Steinernema diaprepesi

by its symbiotic bacteria Xenorhabdus doucetiae to kill insect hosts (Goodrich-Blair and Clarke, 2007; Stock and Blair, 2008; Castillo et al., 2011). The genome of any organism is the basis of the biological, molecular, and cellular processes that are vital for development and reproduction as it encodes the entire inheritance message of living organisms. Improved understanding of the genome aid in the knowledge of complex gene networks, molecular mechanisms of underpinning symbiosis and

Anil Baniya, Jose C. Huguet-Tapia, Peter DiGennaro

Journal of Nematology , 1–4

research-article

A Draft Genome Sequence of the Burrowing Nematode Radopholus similis

Plant-parasitic nematodes cause estimated crop losses of $100 billion worldwide (Coyne et al., 2018). In the United States alone, economic losses due to nematode induced damages have been estimated to be around $10 billion (Coyne et al., 2018). Recent advances in genome sequencing technologies combined with the suite of open source softwares have opened a new paradigm for molecular scientists and plant pathologists to examine the genomic variability that exists within pathogen populations

Reny Mathew, Mark Burke, Charles H. Opperman

journal of nematology , 1–2

research-article

A draft genome for a species of Halicephalobus (Panagrolaimidae)

, free-living species of Panagrolaimidae have lagged behind, as have free-living species of Clade IV (sensu Blaxter et al., 1998) more generally. A major contribution to the genomics of Panagrolaimidae was the genome sequence of Panagrellus redivivus, a species championed for decades as a laboratory model for genetics and development, especially as a satellite to Caenorhabditis elegans (Srinivasan et al., 2013). More recently, several species of Panagrolaimus have been sequenced to study the

Erik J. Ragsdale, Georgios Koutsovoulos, Joseph F. Biddle

journal of nematology , 1–4

research-article

Genome Announcement: The Draft Genomes of Two Radopholus similis populations from Costa Rica

, there have been efforts to better understand the mechanisms behind this nematode’s ability to infect its hosts. These efforts include characterizing cell-wall degrading enzymes (Haegeman et al., 2008; Haegeman et al., 2009), sequencing of the mitochondrial genome (Jacob et al., 2009), and generating multi-stage transcriptomes (Jacob et al., 2008; Huang et al., 2019). The addition of genomes from multiple populations would be beneficial to the community characterizing this nematode. Radopholus

Catherine L. Wram, Cedar N. Hesse, Sulochana K. Wasala, Dana K. Howe, Amy B. Peetz, Dee R. Denver, Danny Humphreys-Pereira, Inga A. Zasada

journal of nematology , 1–4

Research Article

High Mitochondrial Genome Diversity and Intricate Population Structure of Bursaphelenchus xylophilus in Kyushu, Japan

Mitogenomic diversity and genetic population structure of the pinewood nematode (PWN) Bursaphelenchus xylophilus inhabiting Kyushu, Japan were analyzed. A method for performing long PCR using single nematodes and sequencing nematode mitochondrial genomes individually is presented here. About 8 kb (∼55%) of the complete mitochondrial genome was successfully obtained from 285 individuals collected from 12 populations. The 158 single nucleotide polymorphisms detected corresponded to 30 haplotypes

Hanyong Zhang, Erika Okii, Eiji Gotoh, Susumu Shiraishi

Journal of Nematology , ISSUE 3, 281–302

Research Article

PANDEMIC HUMAN CORONAVIRUS – CHARACTERIZATION AND COMPARISON OF SELECTED PROPERTIES OF HCOV-SARS AND HCOV-MERS

ability for human-to-human transmission is higher for HCoV-SARS than for HCoV-MERS (8 generations vs. 4, respectively). Moreover, there are differences in genome structure and pathogenic mechanisms: different receptor, cell entry mechanism, different way of host response modulation (e.g. inhibition of IFNβ cascade), etc. Probably, these differences influence the overall manifestation of the disease in humans. Infection caused by HCoV-MERS might manifest itself as ARDS, a mild-mannered and

Katarzyna W. Pancer

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 22–32

research-article

A genomic resource for the sedentary semi-endoparasitic reniform nematode, Rotylenchulus reniformis Linford & Oliveira

The reniform nematode (Rotylenchulus reniformis Linford & Oliveira) is a sedentary semi-endoparasitic species that infects 77 plant families, including cotton and soybean, and other high-value crops such as sweet potato and pineapple (Robinson et al., 1997). Here, we report the genome sequencing and assembly of R. reniformis. Biological material for sequencing was a R. reniformis stock culture maintained on cotton. Eggs of R. reniformis were collected from infected cotton roots (Hussey and

Kurt C. Showmaker, William S. Sanders, Sebastian Eves-van den Akker, Brigitte E. Martin, Roy N. Platt, John V. Stokes, Chuan-Yu Hsu, Benjamin D. Bartlett, Daniel G. Peterson, Martin J. Wubben

Journal of Nematology , 1–2

original-paper

Impact of Hydrogen on the Transcriptome of Sinorhizobium meliloti 1021 Using RNA-sequencing Technology

synthesized using Second Strand Synthesis Enzyme Mix (New England Biolabs). It was purified using AxyPrep Mag PCR Clean-up (Axygen) and treated with End Prep Enzyme Mix (Axygen) to repair both ends and add dA-tails in a single reaction. Following end-repair and adaptor ligation, RNA-seq data were obtained using a HiSeq instrument (Illumina, San Diego, CA, USA). Data analysis. As the genome of S. meliloti 1021 has already been sequenced, DNA microarrays can be used to study the regulation of gene

RUIRUI LIU, LULU LI, ZHIYING LI, WEIWEI WANG

Polish Journal of Microbiology , ISSUE 1, 39–48

Original Paper

Genetic Analysis Method for Staphylococcus chromogenes Associated with Goat Mastitis

study of several bacterial species. To develop a genotyping method, the genome sequence of Staphylococcus chromogenes MU970 was analysed. The analysis showed nine virulence genes described in Staphylococcus aureus. The MLVA was developed using four loci identified in the genome of S. chromogenes MU970. This genotyping method was examined in 23 strains of CNS isolated from goat mastitis. The rate of discrimination for MLVA was 0.8893, and the highest rates of discrimination per the index of Simpson

ROCÍO A. RUIZ-ROMERO, ROBERTO A. CERVANTES-OLIVARES, ANDRÉS E. DUCOING-WATTY, DANIEL MARTÍNEZ-GÓMEZ, EFRÉN DÍAZ-APARICIO, ESTELA T. MÉNDEZ-OLVERA

Polish Journal of Microbiology , ISSUE 2, –

Article

Analysis and Study the Correlation of Genetic Loci

Liu Yang1,, Bai Xiaojun2, Kang Xiaofeng3

International Journal of Advanced Network, Monitoring and Controls , ISSUE 2, 118–124

research-article

Prevalence of the root lesion nematode virus (RLNV1) in populations of Pratylenchus penetrans from North America

genome (Fig. 1 and Table 1). These isolates were obtained from either established cultures or field collections of P. penetrans, originally collected from agricultural fields distributed throughout several areas of the USA and Canada (Table 1). Pratylenchus penetrans populations were collected mainly from potato and soybean fields, but were also collected from apple, cherry, corn, mint, and raspberry, which is consistent with the wide host range of this nematode species (Castillo and Vovlas, 2007

Paulo Vieira, Amy Peetz, Benjamin Mimee, Kanan Saikai, Dimitre Mollov, Ann MacGuidwin, Inga Zasada, Lev G. Nemchinov

Journal of Nematology , 1–10

Article

Inferring Genome-Wide Gene Regulatory Networks with GPU or CPU Parallel Algorithm

Ming Zheng, Mugui Zhuo, Shugong Zhang, Guixia Liu

International Journal of Advanced Network, Monitoring and Controls , ISSUE 3, 15–19

short-communication

Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52

an adaptative change. WGS of the derivative E. coli strains. Strains 22M and 26M with their original wild-type strain C1 were sent to MicrobesNG at the University of Birmingham for WGS. In silico serotyping analysis using Serotype Finder 1.1 website (https://cge.cbs.dtu.dk/services/Serotype-Finder/) (Joensen et al. 2015) and MLST analysis using MLST 2.0 software (https://pubmlst.org/) (Sepehri et al. 2009) were performed prior to full genome analysis. Results and Discussion In this study, we

AFNAN A. AL-MNASER, MARTIN J. WOODWARD

Polish Journal of Microbiology , ISSUE 1, 121–125

Research Article

INDUSTRIAL APPLICATIONS OF WILD AND GENETICALLY-MODIFIED STRAINS OF ACETIC ACID BACTERIA

both positive and negative aspects. Modifications of the bacterial genome have a significant effect upon the functioning of the entire cell. This review presents industrial applications of metabolites produced by both wild and genetically-modified strains of AAB.

Lidia Stasiak-Różańska, Milena Kupiec

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 4, 398–402

research-article

De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae

patients were identified as A. pegreffii by molecular methods, reflecting their pathogenic potentials to humans (Mattiucci et al., 2013). Recent impressive progress in genome-wide analyses of socio-economically important nematode parasites helped us to better understand the genetic information of them covering the general biology, host-parasite interaction, pathogenicity and development of drug or vaccine candidates. Moreover, the increase of sequencing data have opened a new era in comparative studies

U-Hwa Nam, Jong-Oh Kim, Jeong-Ho Kim

Journal of Nematology , 1–16

research-article

First report of Ovomermis sinensis (Nematoda: Mermithidae) parasitizing fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) in China

Bingjiao Sun, Fen Li, Xiaorui He, Fengqin Cao, Elizabeth Bandason, David Shapiro-Ilan, Weibin Ruan, Shaoying Wu

Journal of Nematology , 1–7

research-article

First report of cultivated Cretan mountain tea (Sideritis syriaca) as a host of Meloidogyne hapla and M. javanica in Crete, with some additional records on the occurrence of Meloidogyne species in Greece

Emmanuel A. Tzortzakakis, Carolina Cantalapiedra-Navarrete, Antonio Archidona-Yuste, Maria Kormpi, Juan E. Palomares-Rius, Pablo Castillo

Journal of Nematology , 1–4

Original Paper

The Prevalence of Exoenzyme S Gene in Multidrug-Sensitive and Multidrug-Resistant Pseudomonas aeruginosa Clinical Strains

Pseudomonas aeruginosa rods are one of the most commonly isolated microorganisms from clinical specimens, usually responsible for nosocomial infections. Antibiotic-resistant P. aeruginosa strains may present reduced expression of virulence factors. This fact may be caused by appropriate genome management to adapt to changing conditions of the hospital environment. Virulence factors genes maybe replaced by those crucial to survive, like antimicrobial resistance genes. The aim of this study was

Tomasz Bogiel, Aleksander Deptuła, Joanna Kwiecińska-Piróg, Małgorzata Prażyńska, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska

Polish Journal of Microbiology , ISSUE 4, 427–431

Research Article

Samplings of Millipedes in Japan and Scarab Beetles in Hong Kong result in five new Species of Pristionchus (Nematoda: Diplogastridae)

. fukushimae on Japanese millipedes. In addition, the authors found three new Pristionchus species on millipedes, which are described as P. riukiariae sp. n., P. degawai sp. n., and P. laevicollis, sp. n., the latter of which was also found on stag beetles. These species are most closely related to P. maxplancki, P. japonicus, and P. quartusdecimus and belong to the pacificus species-complex. The authors describe all species based on morphology, morphometrics, and genome-wide sequence analysis. Mating

Natsumi Kanzaki, Matthias Herrmann, Kohta Yoshida, Christian Weiler, Christian Rödelsperger, Ralf J. Sommer

Journal of Nematology , ISSUE 4, 587–610

Article

LYSSAVIRUS SPP. – RABIES VIRUSES AS A STILL-PRESENT PROBLEM

and the lipoprotein envelope, which is responsible for the overall shape of the virus and is involved in budding – detachment of viral particles from host cells [65]. The N nucleoprotein is the main structural protein of the virus, protecting its RNA against ribonucleases, thus ensuring that it remains in the configuration allowing for transcription [32]. The virus structure is schematically shown in Fig. 1. Fig. 1. The structure of viruses belonging to the genus Lyssavirus spp. 3.2. Genome

Przemysław Gałązka, Patryk Kaczor, Klaudyna Grzelakowska, Kamil Leis

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 153–164

Research Article

FARMED AND COMPANION ANIMALS AS RESERVOIRS OF ZOONOTIC ROTAVIRUS STRAINS

Rotavirus (RV) infections are a major epidemiological problem in humans and farm animals. So far, a number of human and animal RV strains have been identified. Based on the antigenic properties of the VP6 capsid protein, they have been classified into eight serogroups (A-H). The most important of them are viruses from group A (RVA), which are responsible for more than 90% of cases of rotaviral diarrhoea. The segmented structure of the virus genome and the presence of animals in human

Iwona Kozyra, Artur Rzeżutka

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 156–166

Research Article

THE APPLICATION OF GENOTYPING AND PHENOTYPING TECHNIQUES FOR EPIDEMIOLOGICAL ANALYSIS OF MICROORGANISMS

. This review discusses and compares methods facilitating bacterial typing at a strain level. Phenotyping methods analysed in this article are: Biotyping, Antimicrobial Susceptibility Typing, Phage Typing and protein-based methods. Genotyping techniques reviewed in this article are based on digestion of genomic DNA, methods using amplification of DNA, and based on sequencing DNA. This would include Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS). Methods used in identification of

Marcin Brzozowski, Paweł Kwiatkowski, Joanna Jursa-Kulesza, Danuta Kosik-Bogacka

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 353–366

research-article

Two new Species of Pristionchus (Nematoda: Diplogastridae) include the Gonochoristic Sister Species of P. fissidentatus

sister group to all described beetle-associated Pristionchus species. Thus, the gonochorist P. paulseni sp. n. has a crucial position in the phylogeny of Pristionchus. In order to characterize both species, morphology, morphometrics, mating experiments and genome-wide transcriptome sequencing are used. Materials and methods Nematode cultivation One strain of P. paulseni sp. n. was isolated from an adult of Lucanus dybowski taiwanus (Miwa, 1937) (Coleoptera: Lucanidae) collected at Pilu sacred tree

Matthias Herrmann, Natsumi Kanzaki, Christian Weiler, Kohta Yoshida, Christian RÖdelsperger, Ralf J. Sommer

Journal of Nematology , 1–14

Article

THE RHIZOSPHERE MICROBIOME AND ITS BENEFICIAL EFFECTS ON PLANTS – CURRENT KNOWLEDGE AND PERSPECTIVES

Małgorzata Woźniak, Anna Gałązka

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 59–69

research-article

Active and inactive forms of biotin synthase occur in Heterodera glycines

, horizontally transferred genes coding for non-secreted proteins/enzymes with bacterial homology could also play a critical role in SCN biology, parasitism, and (a)virulence. Studies on non-secreted plant-parasitic nematode proteins have been few in number until Craig et al. (2008, 2009) showed that a certain group of non-secreted HGT proteins can include enzymes involved in vitamin B1, B5, B6, and B7 biosynthesis and their corresponding salvage pathways. More recently, a whole genome allelic imbalance

Khee Man Kwon, Sadia Bekal, Leslie L. Domier, Kris N. Lambert

Journal of Nematology , 1–12

Original Paper

The Very Low Frequency of Epstein-Barr JC and BK Viruses DNA in Colorectal Cancer Tissues in Shiraz, Southwest Iran

extracted, then qualified samples introduced to polymerase chain reaction (PCR). The EBV, JCV and BKV genome sequences were detected using specific primers by 3 different in-house PCR assays. Out of 210 subjects, 98 cases were female and the rest were male. The mean age of the participants was 52 ± 1.64 years. EBV and JCV DNA was detected just in one (1.42%) out of seventy adenocarcinoma colorectal tissues. All adenomatous polyp and normal colorectal tissues were negative for EBV and JCV DNA sequences

Jamal Sarvari, Shahab Mahmoudvand, Neda Pirbonyeh, Akbar Safaei, Seyed Younes Hosseini

Polish Journal of Microbiology , ISSUE 1, 73–79

original-paper

Campylobacter fetus is Internalized by Bovine Endometrial Epithelial Cells

has been studied regarding its pathogenicity mechanisms. However, considering the pathogenesis of infection by this organism, it must possess characteristics that allow it to colonize or invade tissues and evade the immune system response. Several virulence factors, such as adhesins, secretion systems, and anti-phagocytic layers, have been identified in the genome of C. fetus (Kienesberger et al. 2014). Nonetheless, it is still necessary to investigate C. fetus interactions with animal hosts. When

LIZETH GUADALUPE CAMPOS-MÚZQUIZ, ESTELA TERESITA MÉNDEZ-OLVERA, BEATRIZ ARELLANO-REYNOSO, DANIEL MARTÍNEZ-GÓMEZ

Polish Journal of Microbiology , ISSUE 2, 217–224

Research Article

Two New Species of Pristionchus (Nematoda: Diplogastridae) from Taiwan and the Definition of the pacificus Species-Complex Sensu Stricto

kurosawai sp. n. that are closely related to P. pacificus. Together with five previously described species they form the “pacificus species-complex sensu stricto” that is characterized by all species forming viable, but sterile F1 hybrids indicating reproductive isolation. P. sikae sp. n. and P. kurosawai sp. n. have a gonochorist mode of reproduction and they are described using morphology, morphometrics, mating experiments, and genome-wide sequence analysis. We discuss the extreme diversification in

Kohta Yoshida, Matthias Herrmann, Natsumi Kanzaki, Christian Weiler, Christian Rödelsperger, Ralf J. Sommer

Journal of Nematology , ISSUE 3, 355–368

research-article

PCR amplification of a long rDNA segment with one primer pair in agriculturally important nematodes

Eukaryotic nuclear ribosomal DNA (rDNA) is arranged in tandem repeat arrays in the genome. Each repeat unit consists of one copy of small subunit (SSU) 18S, internal transcribed spacers (ITS1 and ITS2), 5.8S, and large subunit (LSU) 28S rDNA, and is separated by an external transcribed spacer (EST) and an intergenic spacer (IGS) (Hillis and Dixon, 1991). The copy number of the repeats within most eukaryotic genomes is high, which provide large quantities of template DNA for PCR. In

L. K. Carta, S. Li

Journal of Nematology , 1–8

Research Article

Secondary Metabolites Produced by Heterorhabditis Symbionts and Their Application in Agriculture: What We Know and What to Do Next

genomes. Furthermore, genome mining and characterization of biosynthetic pathways, have uncovered the richness of these compounds, which are predicted to vary across different Photorhabdus spp. and strains. Although progress has been made in the identification and function of SM genes and gene clusters, the targeted testing for the bioactivity of molecules has been scarce or mostly focused on medical applications. In this review, we summarize the current knowledge of Photorhabdus SM, emphasizing on

S. PATRICIA STOCK, AYAKO KUSAKABE, ROUSEL A. OROZCO

Journal of Nematology , ISSUE 4, 373–383

Original Paper

Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland

, stx2g. Among STEC O157 strains we observed conserved core set of 14 virulence factors, stable in bacteria genome at long intervals of time. There was one cattle STEC isolate which possessed verotoxin gene as well as sta1 gene encoded heat-stable enterotoxin STIa characteristic for enterotoxigenic E. coli. To the best of our knowledge, this is the first comprehensive analysis of virulence gene profiles identified in STEC strains isolated from human, cattle and food in Poland. The results obtained

Aleksandra Januszkiewicz, Waldemar Rastawicki

Polish Journal of Microbiology , ISSUE 3, 261–269

Original Paper

Isolation of Sabin-like Polioviruses from Sewage in Poland

different serotypes, yielding a total of 36 PVs. The microneutralization test revealed the presence of 7, 10 and 19 strains belonging to poliovirus serotype 1, 2 and 3, respectively. The genomic variability of 36 poliovirus strains was examined by the restriction fragment length polymorphism assay (RFLP). By combined analyses of two distant, polymorphic segments of the viral genome, one situated in the capsid protein VP1 coding region and the other in the 3D-polymerase coding region, we screened for the

Agnieszka Figas, Magdalena Wieczorek, Anna Żuk-Wasek, Bogumiła Litwińska

Polish Journal of Microbiology , ISSUE 1, 89–96

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