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Research Article | 03-September-2018

Molecular Characterization and Phylogeny of Ditylenchus weischeri from Cirsium arvense in the Prairie Provinces of Canada

Ditylenchus weischeri that parasitizes the weed Cirsium arvense (L.) Scop., 1772, (creeping thistle) was described in 2011 from Russia based on their morphology, ITS-RFLP analysis, and Hsp90 gene sequence of a few individuals and one field collection of the plant. More recently, we found C. arvense parasitized by D. weischeri in the Prairie Provinces of Canada. Plant host preference for D. weischeri was also distinct from D. dipsaci (Kühn) Filipjev, 1936. In the current study, a comprehensive

Mehrdad Madani, Mario Tenuta

Journal of Nematology, Volume 50 , ISSUE 2, 163–182

original-paper | 09-March-2021

Effects of Different Ambient Temperatures on Caecal Microbial Composition in Broilers

cervical dislocation. The caecal contents and liver samples from all 36 broilers were obtained and immediately stored in liquid nitrogen for further 16S rRNA amplicon sequencing and quantitative real-time polymerase chain reaction (qPCR). Quantitative real-time PCR. qPCR analysis was performed to compare the relative expression level of Hsp70, Hsp90, tumor necrosis factor alpha (TNF-α), and NF-κB signaling pathway-related genes (NFKB1, NFKB2) in the three temperature groups. Total RNA from the 36 liver


Polish Journal of Microbiology, Volume 70 , ISSUE 1, 33–43

research-article | 30-November-2020

Characterization of Globodera ellingtonae Populations from Chile Utilizing Whole Genome Sequencing

Oregon showed that the population was distinct from G. pallida, G. rostochiensis, and G. tabacum (Skantar et al., 2011). In this same study, a phylogenetic analysis based on the ITS1 and -2 rDNA indicated that the G. ellingtonae population from Oregon was most similar to Globodera sp. populations collected in Argentina and Chile. A subsequent study on a G. ellingtonae population from Argentina obtained similar results based on HSP90 and ITS regions (Lax et al., 2014). Additionally, in these analyses

C.N. Hesse, I. Moreno, O. Acevedo Pardo, H. Pacheco Fuentes, E. Grenier, L. M. Dandurand, I. A. Zasada

Journal of Nematology, Volume 53 , 1–9

research-article | 30-November-2020

First report of Cactodera milleri Graney and Bird, 1990 from Colorado and Minnesota

county, Colorado, USA. A, B: J2 anterior ends; C, D: J2 tails; E: J2 lateral field; F: Cyst posterior (ventral view); G: Cyst cone mount. Representative J2 from two CO isolates and three MN isolates were used for molecular confirmation of the species, using two ribosomal genes (internal transcribed spacer, ITS 1 and 2 and large ribosomal subunit, 28S), one nuclear gene (partial heat shock protein 90, Hsp90), and one mitochondrial gene (partial cytochrome oxidase I, COI). Markers were amplified

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Saad L. Hafez, Maria N. Hult, Kathryn Kromroy, Kimberly Sigurdson, Michelle Grabowski

Journal of Nematology, Volume 53 , 1–7

research-article | 30-March-2020

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

DNA Polymerase (Thermo Fisher), 0.5 µl PicoMaxx Taq, and 3 µl DNA extract. Cycling conditions were as described previously (Subbotin, 2015). Hsp90: Heat shock protein 90 (Hsp90) fragments were amplified with degenerate primers U288 [5′-GAYACVGGVATYGGNATGACYAA-3′] and L1110 [5′-TCRCARTTVTCCATGATRAAVAC-3′] (Skantar and Carta, 2004). Cycling was performed with 1× PicoMaxx reaction buffer, 0.2 mM dNTPs, 1.5 mM MgCl2, 0.3 µM each primer, 1.25 U PicoMaxx Taq, 1 U Platinum Taq, and 3 µl nematode DNA

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Lynn K. Carta, Jamal Faghihi, Virginia Ferris

Journal of Nematology, Volume 52 , 1–8

research-article | 30-November-2018

Meloidogyne aegracyperi n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing yellow and purple nutsedge in New Mexico

and samples were well mixed then stored at −20°C prior to PCR. Sequence-based identification of individual nematodes was performed using 18S rRNA and heat shock protein 90 (Hsp90) markers with primers used for amplification and sequencing listed in Table 1. Amplification of the 18S rRNA was performed using two primer sets; 79 F deg plus 1629 R deg which amplifies the majority of the 18S rRNA gene and 983 F deg plus Nema 28S R AG which amplifies the 3′ ~1/2 of the 18S rRNA gene and the ITS region

J. D. Eisenback, L. A. Holland, J. Schroeder, S. H. Thomas, J. M. Beacham, S. F. Hanson, V. S. Paes-Takahashi, P. Vieira

journal of nematology, Volume 51 , 1–16

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