Article | 16-October-2019
Dithiothreitol (DTT) and 2-mercaptoethanol (2-ME) are sulfhydryl compounds that can be used to treat serum or plasma to denature IgM antibody reactivity. By using sulfhydryl agents, IgG and IgM antibodies can be separated, the relative amount of IgM and IgG antibodies can be determined, and the risk of hemolytic disease of the fetus and newborn can be assessed.
Lorraine N. Blagg
Immunohematology, Volume 34 , ISSUE 4, 135–139
Short Communication | 15-March-2016
A total of 52 serum samples from patients with symptoms suggestive of tick-borne encephalitis virus (TBEV) infection and positive IgM and/or IgG antibodies were tested for IgG avidity. Acute/recent TBEV infection was confirmed by low/borderline avidity index (AI) in 94.8% IgM positive/IgG positive samples, while in 5.2% high AI was found indicating persisting IgM antibodies. Majority of IgM negative/IgG positive samples (78.6%) showed high AI consistent with past TBEV infection. However, in
Polish Journal of Microbiology, Volume 65 , ISSUE 1, 119–121
Article | 15-February-2021
freeze–thaw elution methods.
Preparing the Eluate
Before performing the elution, the sensitized RBCs must be washed5,6 thoroughly four to six times with large volumes of saline. Use ice-cold saline to prevent dissociation of cold-reactive IgM antibodies. The wash step is critical for removing cell-free antibody while retaining RBC-bound antibody. After the last wash is performed, harvest the supernatant wash into a clean, labeled test tube.
Place an equal volume of washed packed RBCs and
Immunohematology, Volume 35 , ISSUE 2, 45–47
Article | 17-November-2020
Some solid phase red cell adherence (SPRCA) assays are designed to detect IgG antibodies to red blood cell (RBC) antigens. These assays use anti-IgG-coated red cells as the indicator. It is reported that most antibodies to Lea, Leb, P1, M, and N fail to react by solid phase (SP), presumably because they are IgM antibodies. Those detected are assumed to be IgG. In one year, during routine testing using SPRCA to screen patients for intended RBC transfusion, 28 of 59 such examples were found
Immunohematology, Volume 11 , ISSUE 3, 78–80
Article | 13-April-2020
Within the UK blood transfusion services, there is currently no recommendation for the use of either clotted or anticoagulated samples for antibody identification testing. This report describes three cases in which the detection of IgM antibodies was impeded by the use of anticoagulated samples. Two patient samples,referred for compatibility testing, were both identified as having IgM complement-activating anti-S and the remaining case involved an antenatal patient with IgM complement
Amanda J Sweeney
Immunohematology, Volume 22 , ISSUE 2, 72–77
Article | 03-November-2020
unique binding site, a diverse array of immunoglobulin is produced not only by random recombination of the genetic components during assembly of the molecule but also due to somatic mutation. These events, as well as gene restriction, which is often seen in IgM antibodies to blood group antigens, are discussed and some of the differences between monoclonal and polyclonal antibodies are addressed.
Immunohematology, Volume 14 , ISSUE 1, 12–18
Original Paper | 10-December-2018
husbandry, veterinary work or butchery). Enzyme-linked immunoassays were used to detect anti-HEV IgG and IgM in the blood samples. The prevalence of anti-HEV IgG antibodies was 3.0% (12/400), while the prevalence of anti-HEV IgM antibodies was 0.25% (1/400). The prevalence of anti-HEV IgG amongst the samples received from females was approximately 2.5-fold higher than samples received from males (2.4%). Anti-HEV IgG was detected amongst 7% of animal husbandry workers and amongst 2% of veterinarians and
Polish Journal of Microbiology, Volume 67 , ISSUE 4, 525–528
Article | 31-December-2020
A 65-year-old woman was found to have severe autoimmune hemolytic anemia. The patient was group A1, RhoD positive. The direct antiglobulin test was strongly positive with anti-C3 and negative with anti-IgG. The serum contained two distinct IgM antibodies, auto-anti-I and auto-anti-AI. Both were reactive at 22°C. However, the anti-AI also was reactive in saline and in albumin at 37°C. An eluate revealed anti-AI and a weak anti-I. Sequential 51Chromium survival studies were done with
May H. McGinniss,
Richard J. Davey,
Richard A. Binder,
Arthur N. Kales
Immunohematology, Volume 3 , ISSUE 2, 20–22
Article | 03-November-2020
column technology and by LISS tube technique. Both methods detected antibodies directed at common RBC antigens, high-incidence and low-incidence RBC antigens, and warm-reacting autoantibodies. IgM antibodies were not detected by affinity column technology. Affinity column technology compares favorably with the LISS tube technique for IgG antibody detection and identification.
Kayla D. Champagne,
Immunohematology, Volume 14 , ISSUE 4, 149–151
original-paper | 27-March-2019
examination has led to consider other diagnostic techniques more useful in the diagnostic workup of brucellosis.
Serologically, ELISA is the most popular and widely used diagnostic assay. Brucella-specific IgM antibodies are produced in the first week after the disease onset, reaching a maximum after two months. On the other hand, IgG antibodies are detected after the second week of infection, attaining a peak level of six to eight weeks later. While IgG response coincides more closely with the clinical
REEM AL JINDAN,
SHEIKH MUHAMMAD AMJAD
Polish Journal of Microbiology, Volume 68 , ISSUE 1, 51–57