Article | 03-December-2017
, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.
RAJAN SALALIA,
R. K. WALIA,
VISHAL SINGH SOMVANSHI,
PUNEET KUMAR,
ANIL KUMAR
Journal of Nematology, Volume 49 , ISSUE 3, 254–267
research-article | 06-November-2020
was stored at −20°C for later use in PCR reactions.
PCR amplification
ITS region
The internal transcribed spacer (ITS) region was amplified using 0.5 µl each of primers TW81 [5′-GTTTCCGTAGGTGAACCTGC-3′] and AB28 [5′-ATTGCTTAAGTTCAGCGGGT-3′] (Joyce et al., 1994). In a PCR tube containing 15 µl of PCR mixture ready to use (Ready-2x-Go with Taqplus; NanohelixTM, Daejeon, Korea) was added 2 µl of nematode DNA extract, 32 µl of triple distilled water, and 0.5 µl of each primer making a total volume
Rose Mwesige,
Eun-Hwa Kim,
Eun-Hyung Park,
Hyoung-Rai Ko
Journal of Nematology, Volume 52 , 1–12
Research Article | 03-September-2018
anus distance); and elongate, conical tail with pointed tip. Nothotylenchus andrassy n. sp. is morphologically similar to five known species of the genus, namely Nothotylenchus geraerti, Nothotylenchus medians, Nothotylenchus affinis, Nothotylenchus buckleyi, and Nothotylenchus persicus. The results of molecular analysis of rRNA gene sequences, including the D2–D3 expansion region of 28S rRNA, internal transcribed spacer (ITS) rRNA and partial 18S rRNA gene are provide for the new species.
Parisa Jalalinasab,
Mohsen Nassaj Hosseini,
Ramin Heydari
Journal of Nematology, Volume 50 , ISSUE 2, 219–228
Article | 21-July-2017
-stage juveniles lack a stylet, the pharynx degenerated, and can be differentiated into preadult females and males based on the position of the genital primordia. The third-stage juveniles are similar to females but smaller. Phylogenetic studies using the rDNA small subunit 18S, large subunit 28S D2/D3, and internal transcribed spacer (ITS) sequences collectively provide evidence of a grouping with other Gracilacus and some species of Paratylenchus with stylet length of females longer than 41 mm
QING YU,
WEIMIN YE,
TOM POWERS
Journal of Nematology, Volume 48 , ISSUE 3, 203–213
Original Research | 18-July-2017
infective forms, by a broadly rounded tail end in mycetophagous females and lateral fields with 11 to 12 lines midbody in both mycetophagous females and males. The partial 18S, complete internal transcribed spacer, and partial 28S D2/D3 rRNA genes were amplified and sequenced. Phylogenetic analyses of the genes grouped the new species with previously sequenced species of Deladenus in a fully supported clade. This is the first report of Deladenus species with a known infective stage to have the excretory
Qing Yu,
Jianfeng Gu,
Weimin Ye,
Rusong Li,
Jie He
Journal of Nematology, Volume 49 , ISSUE 2, 168–176
Article | 21-July-2017
-pharynx (D% = 46). Hyaline layer occupies approximately half of tail length. Male spicules slightly to moderately curved, with a sharp tip and golden brown in color. The first generation of males lacking a mucron on the tail tip while the second generation males with a short filamentous mucron. Genital papillae with 11 pairs and one unpaired preanal papilla. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions (D2-D3) of the ribosomal
HARUN CIMEN,
VLADIMI´R PU°zA,
JIRI NERMUT,
JUSTIN HATTING,
TSHIMA RAMAKUWELA,
SELCUK HAZIR
Journal of Nematology, Volume 48 , ISSUE 3, 148–158
Research Article | 31-May-2018
Three populations of neotylenchid nematodes were isolated in Ningbo, P. R. China, from white pine lumber (Pinus monticola) imported from the USA. The nematodes were morphologically intermediate between Hexatylus and Deladenus. The nematodes were molecularly characterized based on sequences of the rDNA small subunit 18S, large subunit 28S D2/D3, and internal transcribed spacer sequences. The phylogenetic inferences placed the nematodes with other neotylenchid nematodes, i.e., Fergusobia and
Qing Yu,
Maria Munawar,
Jianfeng Gu,
Weimin Ye
Journal of Nematology, Volume 50 , ISSUE 1, 69–76
Research Article | 03-September-2018
Barbara L. Caoili,
Romnick A. Latina,
Regina Faye C. Sandoval,
Joey I. Orajay
Journal of Nematology, Volume 50 , ISSUE 2, 99–110
Article | 21-July-2017
, joins together anterior to the spicules, and is partially extended and decorated with microvilli. The spicules are incompletely separated, and the tail does not extend beyond the bursa. Phylogenetic trees of 18S rDNA and internal transcribed spacer indicate that the new species belongs to the insectivora group of the genus Oscheius; it is most closely related to O. myriophilus, and the two species can be distinguished on the basis of their different body length, morphological
GUIXIN ZHOU,
HUAN YANG,
FENG WANG,
HAORAN BAO,
GUOXIANG WANG,
XIANGLONG HOU,
JIAN LIN,
GABRIEL YEDID,
KEYUN ZHANG
Journal of Nematology, Volume 49 , ISSUE 1, 33–41
Article | 04-December-2017
sac well developed, 15 (14 to 17) mm long, female tail elongate-conoid with pointed terminus; and male with adanal bursa and spicules 21 to 22 mm long (n = 2). The new species comes close in morphology and morphometrics to five known species of the genus, namely N. affinis, N. hexaglyphus, N. persicus, N. taylori, and N. uniformis. Molecular analyses of the partial 18S, D2/D3 expansion segments of the partial 28S and internal transcribed spacer (ITS) revealed this as a new species. The sequences
MEHRAB ESMAEILI,
RAMIN HEYDARI,
WEIMIN YE
Journal of Nematology, Volume 49 , ISSUE 3, 268–275
Original Paper | 28-December-2016
The endolithic environment is a ubiquitous habitat for microorganisms and a critical interface between biology and geology. In this study, a culture-based method and the phylogenetic analysis based on 16S rRNA and internal transcribed spacer (ITS) sequences were used to investigate the diversity of endolithic bacteria and fungi in two main types of carbonate rocks (namely dolomite and limestone) from Nanjiang Canyon in Guizhou karst area, China. The results of bacterial diversity indicated that
Yuan Tang,
Jian-Zhong Cheng,
Bin Lian
Polish Journal of Microbiology, Volume 65 , ISSUE 4, 413–423
research-article | 17-March-2020
Doreen M. Mgonja,
Gladness E. Temu,
Joseph C. Ndunguru,
Magreth F. Mziray,
Sylvester L. Lyantagaye,
Nessie D. Luambano
Journal of Nematology, Volume 52 , 1–8
research-article | 30-November-2019
transcribed spacer (ITS) 1 & 2 rDNA region was amplified with primers TW81 [5’-GTTTCCGTAGGTGAACCTGC-3’] and AB28 [5’-ATATGCTTAAGTTCAGCGGGT-3’] (Skantar et al., 2012), producing a PCR amplicon of 964 bp. The PCR product was cleaned with the Monarch DNA Gel Extraction Kit (NEB, Ipswitch, MA) and then cloned using the Strataclone PCR Cloning Kit (Agilent, Santa Clara, CA). Cloned plasmid DNA was prepared with the Monarch Plasmid Miniprep Kit (NEB) and sequenced by Genewiz, Inc. Mitochondrial cytochrome
Zafar A. Handoo,
Andrea M. Skantar,
Mihail R. Kantor,
Saad L. Hafez,
Maria N. Hult
Journal of Nematology, Volume 52 , 1–5
research-article | 30-November-2018
conserved, or the non-coding internal transcribed spacer (ITS) regions which are much more variable between species of the same genera (Mckeand, 1998). Sequencing of the ITS regions has revealed species-specific variations, which can be used as diagnostic markers, so enabling accurate identification of species and studies on the phylogenetic relationships between and within species of Pratylenchus (Waeyenberge et al., 2009; Palomares-Rius et al., 2010). Also, the nucleotide sequences of the D2 to D3
Farhana Begum,
John Fosu-Nyarko,
Shashi Sharma,
Bill Macleod,
Sarah Collins,
Michael G. K. Jones
Journal of Nematology, Volume 51 , 1–15
original-paper | 05-December-2019
techniques can overcome these problems (Pryce et al. 2006). The fungal ribosomal RNA gene includes a gene encoding 28S ribosomal DNA, 18S ribosomal DNA, and 5.8S ribosomal DNA, which in the internal transcribed spacer Region1 evolves rapidly (Sirohi et al. 2013; Elekwachi et al. 2017). The last-mentioned gene has the interspecies specificity and intraspecies conservation, and the length of the sequence is moderate enough to get sufficient information (Pryce et al. 2006; Campa et al. 2008; Bellemain et al
HOUFU WANG,
PENGFEI LI,
XUCHUAN LIU,
CHUNYONG ZHANG,
QIONGFEN LU,
DONGMEI XI,
RENHUI YANG,
SHULING WANG,
WENSHUN BAI,
ZHEN YANG,
RONGKANG ZHOU,
XIAO CHENG,
JING LENG
Polish Journal of Microbiology, Volume 68 , ISSUE 4, 505–514
research-article | 30-November-2019
Sedighe Azimi,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–19
Research Article | 31-May-2018
In a search for an entomopathogenic nematode to control cranberry insect pests, three Oscheius populations (Rhabditidae) were recovered through the Galleria-bait method from one sample taken in a wild cranberry marsh in Jackson County, Wisconsin, USA. Morphological studies with light microscopy and scanning electron microscopy, as well as molecular analyses of the near-full-length small subunit rDNA gene, D2/D3 expansion segments of the large subunit rDNA gene, internal transcribed spacer, and
Weimin Ye,
Shane Foye,
Ann E. MacGuidwin,
Shawn Steffan
Journal of Nematology, Volume 50 , ISSUE 1, 9–26
research-article | 30-November-2018
′-ACAAGTACCGTGAGGGAAAGTTG-3′) and reverse D3B (5′-TCGGAAGGAACCAGCTACTA-3′) (Nunn, 1992) primers. The internal transcribed spacer 1 (ITS1) fragment was amplified using the forward primer rDNA1 (5′-TTGATTACGTCCCTGCCCTTT-3′) and the reverse primer rDNA1.58s (5′-ACGAGCCGAGTGATCCACCG-3′) (Subbotin et al., 2000). PCR was carried out for both the aforementioned fragments in a total volume of 40 μl (12 μl distilled water, 20 μl 2x Master mix (Ampliqon, Denmark), 2 μl of each primer (10 pMol/μl), and 4 μl of DNA template). The
Mazdosht Giti,
Leila Kashi,
Majid Pedram
journal of nematology, Volume 51 , 1–11
Article | 21-July-2017
sequences of 28S rDNA D2/D3 and internal transcribed spacer 1 (ITS1) fragments. Compared to Enchodorus neodolichurus, it has basic differences in tail characters and spicule lengths. Molecular phylogenetic studies using partial sequences of 28S rDNA D2/D3 fragment of the new species and available sequences of Nordiidae members and several other dorylaim species/genera, revealed E. yeatsi n. sp. and E. dolichurus forming a clade with 0.81 Bayesian posterior probability (BPP). This
MAJID PEDRAM
Journal of Nematology, Volume 49 , ISSUE 1, 21–26
Research Article | 03-September-2018
. sturhani. The morphological differences of the new species with the aforementioned species are discussed. For all the aforementioned species (except L. protae, currently lacking molecular data) the differences of the new species was also confirmed with differences in molecular sequences of D2-D3 expansion domains of 28S rDNA and the corresponding phylogenetic analyses. The partial sequence of the internal transcribed spacer 1 (ITS1) of the new species was also used in phylogenetic analyses. In partial
Farshad Gharibzadeh,
Ebrahim Pourjam,
Majid Pedram
Journal of Nematology, Volume 50 , ISSUE 2, 207–218
Research Article | 03-September-2018
molecular analysis of many D. weischeri specimens from Canada is presented. Individuals from 41C. arvense or yellow pea grain samples with seeds of C. arvense from the Prairie Provinces were sequenced for the internal transcribed spacer (ITS rDNA), large subunit (LSU) D2D3 28S rDNA, partial segment of small subunit (SSU) 18S rDNA, and the heat shock protein Hsp90 gene. The analysis also included D. weischeri individuals from C. arvense from Russia and garlic with D. dipsaci from the Provinces of Ontario
Mehrdad Madani,
Mario Tenuta
Journal of Nematology, Volume 50 , ISSUE 2, 163–182
research-article | 30-November-2018
J. D. Eisenback,
L. A. Holland,
J. Schroeder,
S. H. Thomas,
J. M. Beacham,
S. F. Hanson,
V. S. Paes-Takahashi,
P. Vieira
journal of nematology, Volume 51 , 1–16
Report | 21-July-2017
identified as Helicotylenchus microlobus according to morphological and morphometric characteristics (Subbotin et al., 2015). DNA was extracted from single nematodes (n = 8) using the Proteinase K method (Kumari and Subbotin, 2012). The internal transcribed spacer (ITS) region of rDNA was amplified with the primers rDNA2/rDNA1.58S (Cherry et al., 1997). The PCR products were then purified and sequenced. The consensus ITS rDNA sequence (accession no. KY271078, 822 bp) that was
GUIPING YAN,
ADDISON PLAISANCE,
DANQIONG HUANG,
ZAFAR A. HANDOO
Journal of Nematology, Volume 49 , ISSUE 1, 1–1
Research Article | 17-October-2018
extracted from single cysts (n = 3) and internal transcribed spacer (ITS) of rRNA and partial cytochrome oxidase I (COI) genes were amplified with primers TW81/AB28 and Het-coxiF/Het-coxiR, respectively (Subbotin et al., 2001; Subbotin, 2015) and sequenced. The resulting sequences were deposited into the GenBank database (Accession no. MG825344 and MG857126) and also subjected to BLAST searches in the database. ITS sequence of H. cyperi showed 100% similarity (100% coverage) with that of a H. cyperi
Abolfazl Hajihassani,
Bhabesh Dutta,
Ganpati B. Jagdale,
Sergei A. Subbotin
Journal of Nematology, Volume 50 , ISSUE 3, 456–458
research-article | 26-April-2019
Abolfazl Hajihassani,
Weimin Ye,
Brooke B. Hampton
Journal of Nematology, Volume 51 , 1–3