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  • Journal Of Nematology

 

Research Article

Nothotylenchus andrassy n. sp. (Nematoda: Anguinidae) from Northern Iran

anus distance); and elongate, conical tail with pointed tip. Nothotylenchus andrassy n. sp. is morphologically similar to five known species of the genus, namely Nothotylenchus geraerti, Nothotylenchus medians, Nothotylenchus affinis, Nothotylenchus buckleyi, and Nothotylenchus persicus. The results of molecular analysis of rRNA gene sequences, including the D2–D3 expansion region of 28S rRNA, internal transcribed spacer (ITS) rRNA and partial 18S rRNA gene are provide for the new species.

Parisa Jalalinasab, Mohsen Nassaj Hosseini, Ramin Heydari

Journal of Nematology , ISSUE 2, 219–228

research-article

Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia

conserved, or the non-coding internal transcribed spacer (ITS) regions which are much more variable between species of the same genera (Mckeand, 1998). Sequencing of the ITS regions has revealed species-specific variations, which can be used as diagnostic markers, so enabling accurate identification of species and studies on the phylogenetic relationships between and within species of Pratylenchus (Waeyenberge et al., 2009; Palomares-Rius et al., 2010). Also, the nucleotide sequences of the D2 to D3

Farhana Begum, John Fosu-Nyarko, Shashi Sharma, Bill Macleod, Sarah Collins, Michael G. K. Jones

Journal of Nematology , 1–15

Article

Morphological and Molecular Characterization of Gracilacus wuae n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip (Heracleum maximum) in Ontario, Canada

-stage juveniles lack a stylet, the pharynx degenerated, and can be differentiated into preadult females and males based on the position of the genital primordia. The third-stage juveniles are similar to females but smaller. Phylogenetic studies using the rDNA small subunit 18S, large subunit 28S D2/D3, and internal transcribed spacer (ITS) sequences collectively provide evidence of a grouping with other Gracilacus and some species of Paratylenchus with stylet length of females longer than 41 mm

QING YU, WEIMIN YE, TOM POWERS

Journal of Nematology , ISSUE 3, 203–213

Article

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.

RAJAN SALALIA, R. K. WALIA, VISHAL SINGH SOMVANSHI, PUNEET KUMAR, ANIL KUMAR

Journal of Nematology , ISSUE 3, 254–267

Article

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

-pharynx (D% = 46). Hyaline layer occupies approximately half of tail length. Male spicules slightly to moderately curved, with a sharp tip and golden brown in color. The first generation of males lacking a mucron on the tail tip while the second generation males with a short filamentous mucron. Genital papillae with 11 pairs and one unpaired preanal papilla. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions (D2-D3) of the ribosomal

HARUN CIMEN, VLADIMI´R PU°zA, JIRI NERMUT, JUSTIN HATTING, TSHIMA RAMAKUWELA, SELCUK HAZIR

Journal of Nematology , ISSUE 3, 148–158

Article

Description of a New Anguinid Nematode, Nothotylenchus phoenixae n. sp. (Nematoda: Anguinidae) Associated with Palm Date Trees and Its Phylogenetic Relations within the Family Anguinidae

sac well developed, 15 (14 to 17) mm long, female tail elongate-conoid with pointed terminus; and male with adanal bursa and spicules 21 to 22 mm long (n = 2). The new species comes close in morphology and morphometrics to five known species of the genus, namely N. affinis, N. hexaglyphus, N. persicus, N. taylori, and N. uniformis. Molecular analyses of the partial 18S, D2/D3 expansion segments of the partial 28S and internal transcribed spacer (ITS) revealed this as a new species. The sequences

MEHRAB ESMAEILI, RAMIN HEYDARI, WEIMIN YE

Journal of Nematology , ISSUE 3, 268–275

Original Paper

Characterization of Endolithic Culturable Microbial Communities in Carbonate Rocks from a Typical Karst Canyon in Guizhou (China)

The endolithic environment is a ubiquitous habitat for microorganisms and a critical interface between biology and geology. In this study, a culture-based method and the phylogenetic analysis based on 16S rRNA and internal transcribed spacer (ITS) sequences were used to investigate the diversity of endolithic bacteria and fungi in two main types of carbonate rocks (namely dolomite and limestone) from Nanjiang Canyon in Guizhou karst area, China. The results of bacterial diversity indicated that

Yuan Tang, Jian-Zhong Cheng, Bin Lian

Polish Journal of Microbiology , ISSUE 4, 413–423

research-article

Molecular and morphological characterization of the amaryllis lesion nematode, Pratylenchus hippeastri (Inserra et al., 2007), from California

transcribed spacer (ITS) 1 & 2 rDNA region was amplified with primers TW81 [5’-GTTTCCGTAGGTGAACCTGC-3’] and AB28 [5’-ATATGCTTAAGTTCAGCGGGT-3’] (Skantar et al., 2012), producing a PCR amplicon of 964 bp. The PCR product was cleaned with the Monarch DNA Gel Extraction Kit (NEB, Ipswitch, MA) and then cloned using the Strataclone PCR Cloning Kit (Agilent, Santa Clara, CA). Cloned plasmid DNA was prepared with the Monarch Plasmid Miniprep Kit (NEB) and sequenced by Genewiz, Inc. Mitochondrial cytochrome

Zafar A. Handoo, Andrea M. Skantar, Mihail R. Kantor, Saad L. Hafez, Maria N. Hult

Journal of Nematology , 1–5

Research Article

Molecular Characterization and Phylogeny of Ditylenchus weischeri from Cirsium arvense in the Prairie Provinces of Canada

molecular analysis of many D. weischeri specimens from Canada is presented. Individuals from 41C. arvense or yellow pea grain samples with seeds of C. arvense from the Prairie Provinces were sequenced for the internal transcribed spacer (ITS rDNA), large subunit (LSU) D2D3 28S rDNA, partial segment of small subunit (SSU) 18S rDNA, and the heat shock protein Hsp90 gene. The analysis also included D. weischeri individuals from C. arvense from Russia and garlic with D. dipsaci from the Provinces of Ontario

Mehrdad Madani, Mario Tenuta

Journal of Nematology , ISSUE 2, 163–182

research-article

Meloidogyne aegracyperi n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing yellow and purple nutsedge in New Mexico

J. D. Eisenback, L. A. Holland, J. Schroeder, S. H. Thomas, J. M. Beacham, S. F. Hanson, V. S. Paes-Takahashi, P. Vieira

journal of nematology , 1–16

Report

First Report of the Spiral Nematode Helicotylenchus microlobus Infecting Soybean in North Dakota

identified as Helicotylenchus microlobus according to morphological and morphometric characteristics (Subbotin et al., 2015). DNA was extracted from single nematodes (n = 8) using the Proteinase K method (Kumari and Subbotin, 2012). The internal transcribed spacer (ITS) region of rDNA was amplified with the primers rDNA2/rDNA1.58S (Cherry et al., 1997). The PCR products were then purified and sequenced. The consensus ITS rDNA sequence (accession no. KY271078, 822 bp) that was

GUIPING YAN, ADDISON PLAISANCE, DANQIONG HUANG, ZAFAR A. HANDOO

Journal of Nematology , ISSUE 1, 1–1

Research Article

First Report of the Yellow Nutsedge Cyst Nematode, Heterodera cyperi, in Georgia, U.S.A.

extracted from single cysts (n = 3) and internal transcribed spacer (ITS) of rRNA and partial cytochrome oxidase I (COI) genes were amplified with primers TW81/AB28 and Het-coxiF/Het-coxiR, respectively (Subbotin et al., 2001; Subbotin, 2015) and sequenced. The resulting sequences were deposited into the GenBank database (Accession no. MG825344 and MG857126) and also subjected to BLAST searches in the database. ITS sequence of H. cyperi showed 100% similarity (100% coverage) with that of a H. cyperi

Abolfazl Hajihassani, Bhabesh Dutta, Ganpati B. Jagdale, Sergei A. Subbotin

Journal of Nematology , ISSUE 3, 456–458

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