of Iranian population of Longidorus proximus (Sturhan and Argo, 1983). In each stage the length of replacement odontostyle is equal to the length of functional odontostyle in the next stage.
Molecular characterization and phylogenetic position of the Iranian population of Longidorus proximus
Sequencing of LSU rDNA D2-D3 expansion domains of two females of L. proximus yielded two single fragments, 701 (fem1, MK795203) and 757 (fem2, MK795204) nt long. The longer size of the latter sequences
journal of nematology , 1–11
Hadi Karimipour Fard,
Journal of Nematology , 1–3
Thi Duyen Nguyen,
Huu Tien Nguyen,
Thi Mai Linh Le,
Quang Phap Trinh
journal of nematology , 1–5
microscope (5 kV) (Zeiss, Oberkochen, Germany).
DNA extraction, PCR, and sequencing
A single live nematode specimen of D. brevis n. sp. was picked out and transferred to a small drop of TE buffer (10 mM Tris-Cl, 0.5 mM EDTA; pH 9.0; Qiagen) on a clean slide and squashed using a clean cover slip. The suspension was collected by adding 15 μl TE buffer. The DNA sample was stored at −20°C until used as PCR template. Three DNA samples were prepared in this manner. Primers for LSU rDNA D2-D3 expansion
Journal of Nematology , 1–13
Two SSU rDNA (MN519728 and MN847693) and two LSU rDNA D2-D3 (MN519727 and MN847694) sequences were generated for the new species using four different female specimens. There were no differences between the two SSU and two LSU rDNA sequences. Subsequently, only one sequence from each fragment was used in corresponding phylogenetic trees.
In the inferred SSU tree using the above-mentioned SSU data set (Fig. 4), the new species placed into the maximally supported clade of Cephalenchus. Within
Gerhard Du Preez,
Journal of Nematology , 1–10
profiles and phylogenetic status
The amplification and sequencing of near full-length SSU and LSU rDNA D2-D3 expansion segments of Seinura italiensis n. sp. yielded two single fragments of 1732 and 801 nucleotides long. The BLAST search using the SSU sequence revealed that it has 96.92% identity with the SSU rDNA of S. caverna (LC414971). The identity value of other sequences that showed high coverage was all less than 93%. The BLAST search using the LSU D2-D3 sequence revealed it has 91.01% identity
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of small subunit (SSU) and large subunit (LSU) rDNA D2/D3 fragments, the new species formed a clade with two currently available GenBank-derived, unspecified isolates/sequences in SSU and three other isolates/sequences in LSU trees, respectively.
Journal of Nematology , ISSUE 2, 223–230