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  • Journal Of Nematology


research-article | 30-November-2018

Data of an Iranian Population of L. proximus Sturhan & Argo, 1983, with taxonomic revision of L. israelensis Peneva, Orion, Shlevin, Bar-Eyal & Brown, 1998 (Nematoda: Longidoridae) and Proposal for a New Synonymy

of Iranian population of Longidorus proximus (Sturhan and Argo, 1983). In each stage the length of replacement odontostyle is equal to the length of functional odontostyle in the next stage. Molecular characterization and phylogenetic position of the Iranian population of Longidorus proximus Sequencing of LSU rDNA D2-D3 expansion domains of two females of L. proximus yielded two single fragments, 701 (fem1, MK795203) and 757 (fem2, MK795204) nt long. The longer size of the latter sequences

Mazdosht Giti, Leila Kashi, Majid Pedram

journal of nematology, Volume 51 , 1–11

research-article | 30-November-2020

Laimaphelenchus africanus n. sp. (Tylenchomorpha: Aphelenchoididae) from South Africa, a morphological and molecular phylogenetic study, with an update to the diagnostics of the genus

. The specimens were then transferred to two individual Eppendorf tubes containing 15 µl ddH2O and their respective DNA was extracted using the chelex-100 protocol of Rashidifard et al. (2019). The DNA samples were stored at –20°C until used for amplification. The partial sequences of the large subunit ribosomal DNA (LSU rDNA D2-D3) were amplified using forward primer D2A (5′–ACAAGTACCGTGAGGGAAAGT–3′) and reverse primer D3B (5′–TCGGAAGGAACCAGCTACTA–3′) (Nunn, 1992). The polymerase chain reaction (PCR

Farahnaz Jahanshahi Afshar, Milad Rashidifard, Joaquín Abolafia, Miloslav Zouhar, Hendrika Fourie, Majid Pedram

Journal Of Nematology, Volume 53 , 1–14

research-article | 13-April-2020

First molecular characterization of an Iranian population of Schistonchus caprifici (Gasparrini, 1864) Cobb, 1927 (Rhabditida; Aphelenchoidea)

Hadi Karimipour Fard, Hamid Zare

Journal of Nematology, Volume 52 , 1–3

research-article | 30-November-2020

Morphological and molecular characterization of Filenchus pseudodiscus n. sp. from east Golestan province, north Iran; with an updated phylogeny of Malenchus Andrássy, 1968 (Tylenchomorpha: Tylenchidae)

province (Jalalinasab et al., 2019). Molecular phylogenetic status The amplification and sequencing of SSU and LSU rDNA (D2-D3 region) fragments of the new species yielded single fragments of 788 and 726 nt long (accession numbers MW346650 and MW346649, respectively). The BLAST search using the SSU sequence, revealed that its highest identity (99.37%) with currently available SSU sequences of Tylenchidae belonged to F. vulgaris (KJ869307) and Filenchus sp. (JQ814877). However, the BLAST search using

Parnaz Mortazavi, Fariba Heydari, Joaquín Abolafia, Pablo Castillo, Majid Pedram

Journal of Nematology, Volume 53 , 1–14

research-article | 30-November-2018

First report of Longidorus mindanaoensis Coomans, De Ley, Jimenez and De Ley, 2012 (Nematoda: Longidoridae) From a Mangrove Forest in Vietnam

Thi Duyen Nguyen, Huu Tien Nguyen, Thi Mai Linh Le, Neriza Nobleza, Quang Phap Trinh

journal of nematology, Volume 51 , 1–5

research-article | 24-April-2020

Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

variable. Two SSU rDNA (MN519728 and MN847693) and two LSU rDNA D2-D3 (MN519727 and MN847694) sequences were generated for the new species using four different female specimens. There were no differences between the two SSU and two LSU rDNA sequences. Subsequently, only one sequence from each fragment was used in corresponding phylogenetic trees. In the inferred SSU tree using the above-mentioned SSU data set (Fig. 4), the new species placed into the maximally supported clade of Cephalenchus. Within

Milad Rashidifard, Gerhard Du Preez, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–10

research-article | 18-March-2020

Description of Seinura italiensis n. sp. (Tylenchomorpha: Aphelenchoididae) found in the medium soil imported from Italy

profiles and phylogenetic status The amplification and sequencing of near full-length SSU and LSU rDNA D2-D3 expansion segments of Seinura italiensis n. sp. yielded two single fragments of 1732 and 801 nucleotides long. The BLAST search using the SSU sequence revealed that it has 96.92% identity with the SSU rDNA of S. caverna (LC414971). The identity value of other sequences that showed high coverage was all less than 93%. The BLAST search using the LSU D2-D3 sequence revealed it has 91.01% identity

Jianfeng Gu, Munawar Maria, Lele Liu, Majid Pedram

Journal of Nematology, Volume 52 , 1–11

research-article | 30-November-2019

Description of Deladenus brevis n. sp. (Sphaerularioidea: Neotylenchidae) from Iran: a morphological and molecular phylogenetic study

microscope (5 kV) (Zeiss, Oberkochen, Germany). DNA extraction, PCR, and sequencing A single live nematode specimen of D. brevis n. sp. was picked out and transferred to a small drop of TE buffer (10 mM Tris-Cl, 0.5 mM EDTA; pH 9.0; Qiagen) on a clean slide and squashed using a clean cover slip. The suspension was collected by adding 15 μl TE buffer. The DNA sample was stored at −20°C until used as PCR template. Three DNA samples were prepared in this manner. Primers for LSU rDNA D2-D3 expansion

Fariba Heydari, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–13

Article | 24-July-2017

Cryptaphelenchus varicaudatus n. sp. (Rhabditida: Ektaphelenchinae) from Tehran Province, Iran

of small subunit (SSU) and large subunit (LSU) rDNA D2/D3 fragments, the new species formed a clade with two currently available GenBank-derived, unspecified isolates/sequences in SSU and three other isolates/sequences in LSU trees, respectively.


Journal of Nematology, Volume 49 , ISSUE 2, 223–230

research-article | 30-November-2020

An interesting rare tylenchid species, Antarctenchus urmiensis n. sp. (Tylenchomorpha; Psilenchidae) from Urmia Lake islands, northwest Iran, with a discussion on the taxonomy of related genera

forward primer 22F (5´–TCCAAGGAAGGCAGCAGGC–3´) and revers primer 13R (5´–GGGCATCACAGACCTGTTA–3´) (Dorris et al., 2002). Sometimes the reverse primer 1573 R (5´–TACAAAGGGCAGGGACGTAAT–3´) (Mullin et al., 2005) was used interchangeably. The LSU rDNA D2-D3 expansion segments were amplified using the forward D2A (5´–ACAAGTACCGTGAGGGAAAGTTG–3´) and reverse D3B (5’–TCGGAAGGAACCAGCTACTA–3´) (Nunn, 1992) primer pairs. The PCR products were sequenced in both directions using the same primers used in PCR with an

Mohammad Amiri Bonab, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 53 , 1–14

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