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Article | 30-November-2019

APPLICATION OF THE MALDI-TOF MS TECHNIQUE FOR IDENTIFICATION OF DERMATOPHYTES

struktury morfologicznej uzyskanych kolonii i ich charakterystyka mikroskopowa [22, 47]. Konwencjonalne metody identyfikacji dermatofitów są zatem pracochłonne i ze względu na wysoki stopień podobieństwa morfologicznego między niektórymi gatunkami tej grupy grzybów wymagają dużej wiedzy i doświadczenia diagnosty laboratoryjnego [15, 30]. Dodatkowo, czas uzyskania wyniku badania z wykorzystaniem tych metod może przekraczać nawet 5 tygodni [52]. Metoda MALDI-TOF MS (Matrix-Assisted Laser Desorption

Sebastian Gnat, Dominik Łagowski, Aneta Nowakiewicz

Postępy Mikrobiologii - Advancements of Microbiology, Volume 59 , ISSUE 3, 315–324

Short Communication | 15-March-2016

Usefulness of CHROMagar Candida Medium, Biochemical Methods – API ID32C and VITEK 2 Compact and Two MALDI-TOF MS Systems for Candida spp. Identification

This study was conducted to compare of the yeasts identification results obtained with two new systems using the MALDI-TOF MS technique with the ones obtained using the routine identification methods of Candida spp. in clinical microbiology laboratories. All 124 Candida spp. isolates were recovered from the routine examination of clinical specimens in microbiological laboratories and collected in the Centre of Quality Control in Microbiology in Warsaw (Poland). Our findings confirm the high

Elżbieta Stefaniuk, Anna Baraniak, Monika Fortuna, Waleria Hryniewicz

Polish Journal of Microbiology, Volume 65 , ISSUE 1, 111–114

Original Paper | 09-March-2018

MALDI-TOF MS Detection of Endophytic Bacteria Associated with Great Nettle (Urtica dioica L.), Grown in Algeria

). The identification of bacteria is done by biochemical tests and confirmed by MALDI-TOF MS. Seven genus and eleven species were isolated from the Great Nettle. They belong to the genera Bacillus, Escherichia, Pantoea, Enterobacter, Staphylococcus, Enterococcus and Paenibacillus. The majority of these bacteria were isolated from Tlemcen which makes this region the richest in endophytic bacteria compared to that harvested from Dellys. The results show also that the leaves are the most diversified in

Souheyla Toubal, Ouahiba Bouchenak, Djillali Elhaddad, Karima Yahiaoui, Sarah Boumaza, Karim Arab

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 67–72

original-paper | 31-May-2021

Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry for Identification of Microorganisms in Clinical Urine Specimens after Two Pretreatments

). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a new method for rapid microbial identification without the need for target amplification (Nomura 2015). It can replace methods such as 16S rRNA gene sequencing (Vincent et al. 2013). It has a great potential in identifying bacteria directly from urine samples and culture-positive blood samples (Sauget et al. 2017; Nomura et al. 2020). Nevertheless, this novel method has not been widely used in clinical

DERU LEI, PEIYING CHEN, XUETING CHEN, YUJIE ZONG, XIANGYANG LI

Polish Journal of Microbiology, Volume 70 , 1–7

Article | 26-October-2019

Blood group genotyping: the power and limitations of the Hemo ID Panel and MassARRAY platform

Matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI-TOF MS), is a sensitive analytical method capable of resolving DNA fragments varying in mass by a single nucleotide. MALDI-TOF MS is applicable to blood group genotyping, as the majority of blood group antigens are encoded by single nucleotide polymorphisms. Blood group genotyping by MALDI-TOF MS can be performed using a panel (Hemo ID Blood Group Genotyping Panel, Agena Bioscience Inc., San Diego, CA) that is

Rhiannon S. McBean, Catherine A. Hyland, Robert L. Flower

Immunohematology, Volume 31 , ISSUE 2, 75–80

original-paper | 15-May-2020

Comparison of Rapid and Routine Methods of Identification and Antibiotic Susceptibility Testing of Microorganisms from Blood Culture Bottles

tests that are fast, reliable, easy to use, and inexpensive, as well as applicable for use as diagnostic checkpoints is a significant limitation (Tuite et al. 2014; Yis 2015; Caliendo and Hodinka 2017). Although several molecular or proteomic methods (e.g., Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry [MALDI-TOF MS]) exist that can be used to direct evaluation of specimens or colonies, the gold-standard methods with the highest sensitivity and safety still remain

SADIK AKGUN, HAKAN SEZGIN SAYINER

Polish Journal of Microbiology, Volume 69 , ISSUE 2, 165–176

Original Paper | 10-December-2018

Primary and Secondary Bacteremia Caused by Proteus spp.: Epidemiology, Strains Susceptibility and Biofilm Formation

Abstract Proteus spp. is an etiological factor of urinary tract and bloodstream infections. The aim of this study was the retrospective analysis of susceptibility of Proteus spp. strains isolated from bloodstream infections (BSIs) as well as similarity evaluation of the strains isolated from different clinical samples. Proteus spp. strains were isolated in 2009–2017 from hospital patients. Identification was based on the colony’s morphology and biochemical or MALDI-TOF MS analyzes. The

JOANNA KWIECIŃSKA-PIRÓG, KRZYSZTOF SKOWRON, EUGENIA GOSPODAREK-KOMKOWSKA

Polish Journal of Microbiology, Volume 67 , ISSUE 4, 471–478

Original Paper | 15-March-2016

Streptococcus anginosus (milleri) Group Strains Isolated in Poland (1996–2012) and their Antibiotic Resistance Patterns

microbiology laboratory as less virulent “viridans streptococci”. Epidemiological situation regarding SAG infections in Poland has been unrecognized, therefore we performed a retrospective analysis of strains isolated between 1996 and 2012. Strains suspected of belonging to SAG were re-identified using an automated biochemical approach (Vitek2) and MALDI-TOF MS. We performed first analysis of antibiotic resistance among SAG strains isolated in Poland using automated methods (Vitek2), disk

Katarzyna Obszańska, Izabella Kern-Zdanowicz, Aleksandra Kozińska, Katarzyna Machura, Elżbieta Stefaniuk, Waleria Hryniewicz, Izabela Sitkiewicz

Polish Journal of Microbiology, Volume 65 , ISSUE 1, 33–41

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