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-based classifiers from a binary tree classification as a decision-making system. In our framework for molecular robot development, the system components of molecular robots and simple classifier rules were used to alleviate the computational burden. The design consists of a basic model that generates rule-based classifier gates in several binary tree and cancer classifications based on micro (mi)RNA expression. Simulation results showed that detection and classification were rapid using this system

Wibowo Adi, Kosuke Sekiyama

International Journal on Smart Sensing and Intelligent Systems , ISSUE 2, 944–965


Molecular and morphological characterization of the amaryllis lesion nematode, Pratylenchus hippeastri (Inserra et al., 2007), from California

and processed to glycerin by the formalin glycerin method (Golden, 1990; Hooper, 1970). Photomicrographs of the specimens were made with a Nikon Eclipse Ni compound microscope using a Nikon DS-Ri2 camera. Measurements were made with an ocular micrometer on a Leica WILD MPS48, Leitz DMRB compound microscope. All measurements are in micrometers unless otherwise stated. The molecular identification was performed using DNA extracted from single nematodes as template in PCR reactions. The internal

Zafar A. Handoo, Andrea M. Skantar, Mihail R. Kantor, Saad L. Hafez, Maria N. Hult

Journal of Nematology , 1–5


Morphological and Molecular Characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy

Oscheius as well the two monophyletic species groups within the latter. Molecular data of Oscheius sensu lato species, many of them described during the last years, have been matter of analyses by several authors (Félix et al., 2001; Darby et al., 2011; Darsouei et al., 2014; Campos-Herrera et al., 2015; Torrini et al., 2015; Tabassum et al., 2016; Lima de Brida et al., 2017; Valizadeh et al., 2017; Zhou et al., 2017), resulting in the confirmation of the monophyly of the Insectivorus- and the

Joaquín Abolafia, Reyes Peña-Santiago

journal of nematology , 1–21


Molecular and morphological characterization of Paurodontella composticola n. sp. (Nematoda: Hexatylina, Sphaerulariidae) from Iran

, 1980) in the order of Tylenchida (Thorne, 1949). Siddiqi (2000) considered the family Paurodontidae a junior synonym of Sphaerulariidae, since the genera included in these families are morphologically similar and have similar life cycles. However, there is not enough available molecular data to support these statements. Biologically, in Paurodontidae, a fungus-feeding generation is well known and nothing is known about entomoparasitic forms, whereas in Sphaerulariidae, an entomoparasitic form is

Mehrab Esmaeili, Ramin Heydari, Ahmad Kheiri, Weimin Ye

Journal of Nematology , 1–12


Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.


Journal of Nematology , ISSUE 3, 254–267


Morphological and molecular characterization of Pungentus sufiyanensis n. sp. and additional data on P. engadinensis (Altherr, 1950) Altherr, 1952 (Dorylaimida: Nordiidae) from northwest of Iran

and cultivated soils of northwest Iran (East–West Azarbaijan and Kurdistan provinces) to explore the dorylaimid diversity of this region. Their study revealed that they belonged to one new and one known species. The objective of this work was to report Pungentus sufiyanensis n. sp. using morphology, morphometric, and molecular methods and provide new data about P. engadinensis. Materials and methods Extraction and processing of nematodes Soil samples were collected from the rhizosphere of

Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Reyes Peña-Santiago

Journal of Nematology , 1–12


Morphological and Molecular Characteristics of Pratylenchus haiduongensis sp. n., a New Species of Root–Lesion Nematodes Associated with Carrot in Vietnam

terminus observed in some specimens. The matrix code of P. haiduongensis sp. n. is: A2, B1, C4, D(1,3), E1, F(5,6), G(1,2), H(1,4); I(1,2,3,4), J1, K(1,2) according to Castillo and Vovlas (2007). The LSU–D2D3 segment and the ITS–rDNA region of this species were amplified and sequenced. The morphological characters and molecular phylogenetic analyses confirmed that this is a new species of the genus Pratylenchus in Vietnam.


Journal of Nematology , ISSUE 3, 276–285

Research Article


The molecular typing methods are used to identify specific genetic targets and relationships between microbial isolates. In order to understand clonal relatedness between the microbial strains, classic phenotypic methods are used in line with modern molecular biology techniques. The development of genetics, especially new techniques like molecular typing, have revolutionized microbial research. After 1970, the development techniques, especially those referring to DNA sequencing, established

Daria Artyszuk, Tomasz Wołkowicz

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 179–193


Description of Seinura italiensis n. sp. (Tylenchomorpha: Aphelenchoididae) found in the medium soil imported from Italy

isolated by the modified Baermann funnel technique for 24 hr. Permanent slides were prepared by heat-killed and fixed nematodes with FA 4:1 and ethanol-glycerin dehydration according to Seinhorst (1959) as modified by De Grisse (1969). Morphometrics, drawings, and light micrographs of nematodes were done with the aid of a Zeiss microscope equipped with a Zeiss AxioCam MRm CCD camera. Molecular and phylogenetic analyses DNA samples were prepared according to Li et al. (2008). Three sets of primers

Jianfeng Gu, Munawar Maria, Lele Liu, Majid Pedram

Journal of Nematology , 1–11

Original Research

Description of Pseudacrobeles (Pseudacrobeles) curvatus sp. n. (Cephalobidae: Rhabditida) in South Korea

and diagnostic features of Pseudacrobeles species and molecular sequence data from the D2-D3 regions of the 28S ribosomal DNA (rDNA) and ITS1-5.8S-ITS2 region of rDNA from the new species, which can be used as molecular barcode sequences.  

Jiyeon Kim, Taeho Kim, Joong-Ki Park

Journal of Nematology , ISSUE 2, 162–167

Research Article

First report of Bursaphelenchus antoniae from Pinus strobus in the U.S.

Juvenile, female and male nematodes were discovered in wood chips of white pine Pinus strobus from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics. The specimens were identified as Bursaphelenchus antoniae Penas et al., 2006 based on 18S rDNA molecular sequence vs

Lynn K. Carta, R. L. Wick

Journal of Nematology , ISSUE 4, 473–478

Research Article

Improved 18S small subunit rDNA primers for problematic nematode amplification

The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new

L. K. Carta, S. Li

Journal of Nematology , ISSUE 4, 533–542

Original Paper

Modeling alloantibody formation to highincidence red blood cell antigens in immune responders using genotypic data  

Alloimmunization to red blood cell antigens is unpredictable and poorly understood. Patients who are negative for highincidence antigens (HIAs) are at risk for developing the corresponding antibodies. Molecular methods can easily predict the lack of an antigen and thus, the risk of an individual to become immunized. We examined the prevalence and risk factors for HIA alloimmunization in patients at risk based on genotyping results. Genotyping using a molecular method (HEA BeadChip&trade

Patricia A.R. Brunker, Keerthana Ravindran, R. Sue Shirey

Immunohematology , ISSUE 1, 9–14


Morphological and Molecular Identification of Longidorus euonymus and Helicotylenchus multicinctus from the Rhizosphere of Grapevine and Banana in Greece

Plant-parasitic nematodes such as Longidorus euonymus and Helicotylenchus multicintctus are species widely distributed in central Europe as well as in Mediterranean area. In Greece, both species have been previously reported but no morphometrics or molecular data were available for these species. Nematode surveys in the rhizosphere of grapevines in Athens carried out in 2016 and 2017, yielded a Longidorus species identified as Longidorus euonymus. Similarly, a population of Helicotylenchus


Journal of Nematology , ISSUE 3, 233–235

Research Article

Description of Longidorus azarbaijanensis n. sp. (Dorylaimida: Longidoridae) from Iran

Longidorus azarbaijanensis n. sp. is described and illustrated using morphological and molecular data. It was recovered in West Azarbaijan province, northwestern Iran, from the rhizospheric soil of foxtail weed. The new species is characterized by having 5.4 to 6.8 mm long females, offset, anteriorly flat lip region and separated from the rest of the body by a shallow constriction, funnel-shaped amphidial pouches, the guiding ring at 23 to 27 µm from the anterior end, 73 to 81 and 44 to 50 µm

Farshad Gharibzadeh, Ebrahim Pourjam, Majid Pedram

Journal of Nematology , ISSUE 2, 207–218


Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

, 20 km north of the town Lichtenburg (North-West Province, South Africa). In-depth investigations revealed that it belongs to an unknown species, being described herein as C. driekieae n. sp. Therefore, the present study aims to characterize this species using both traditional and molecular techniques and discuss its phylogenetic affinities using SSU and LSU markers. Materials and methods Nematode extraction and morphological studies Nematodes were extracted from the soil using the modified

Milad Rashidifard, Gerhard Du Preez, Joaquín Abolafia, Majid Pedram

Journal of Nematology , 1–10


First Report of Criconema demani from Russia

record of this species in Russia. Materials and Methods Nematodes were extracted from the soil samples using a modification of the decanting and sieving method (Flegg, 1967). For morphological studies, the nematodes were killed with hot water, fixed in 5 % formalin solution, and mounted in glycerin slides using the Seinhorst technique (Seinhorst, 1959). For molecular studies, nematodes were fixed with 96% ethanol. Their total DNA was extracted using the K-Sorb kit (Syntol LLC, Russia) according to

Sergei Tabolin, Irina Markina

Journal of Nematology , 1–4


First report of Bursaphelenchus fungivorus (Nematoda: Aphelenchida) in Italy and an overview of nematodes associated with Crocus sativus L.

time in Italy, and, in particular, associated with C. sativus corms. Morphometrical and molecular characterization of the nematode is given. In addition, a review of nematodes associated with plants of C. sativus worldwide is provided. Material and methods Collecting corms and nematodes extraction In September 2018, 20 corms of Crocus sativus were collected by the Plant Protection Service of Lazio Region on the farm “Arte Zafferano” located in Terracina (Latina, Lazio, Central Italy), in the

Giulia Torrini, Agostino Strangi, Stefania Simoncini, Maria Luppino, Pio Federico Roversi, Leonardo Marianelli

Journal of Nematology , 1–11


Integrative taxonomy of Xiphinema histriae and Xiphinema lapidosum from Spain

population of Xiphinema lapidosum was identified in association with Olea europaea subsp. europaea L. Lamberti et al. (1993a, 1993b) and Roca and Bravo (1993) described female and male stages of X. histriae and X. lapidosum, respectively, but in both species no juvenile stages were detected and described. The objectives of this study were: (i) to provide updated morphological descriptions of juvenile stages of X. histriae and X. lapidosum, (ii) to characterize the molecular data of both species using the

Ruihang Cai, Antonio Archidona-Yuste, Carolina Cantalapiedra-Navarrete, Juan E. Palomares-Rius, Jingwu Zheng, Pablo Castillo

Journal of Nematology , 1–21

Research Article

Nothotylenchus andrassy n. sp. (Nematoda: Anguinidae) from Northern Iran

Nothotylenchus andrassy n. sp. is described and illustrated from moss (Sphagnum sp.) based on morphology and molecular analyses. Morphologically, this new species is characterized by a medium body size, six incisures in the lateral fields, and a delicate stylet (8–9 µm long) with clearly defined knobs. Pharynx with fusiform, valveless, non-muscular and sometimes indistinct median bulb. Basal pharyngeal bulb elongated and offset from the intestine; a long post-vulval uterine sac (55% of vulva to

Parisa Jalalinasab, Mohsen Nassaj Hosseini, Ramin Heydari

Journal of Nematology , ISSUE 2, 219–228

Research paper

Effects of individual stressors used in a battery of “chronic unpredictable stress” on long-term plasticity in the hippocampus of juvenile rats

Alexandra A. Gorbunova, Irina V. Kudryashova, Anna O. Manolova, Margarita R. Novikova, Mikhail Yu. Stepanichev, Natalia V. Gulyaeva

Acta Neurobiologiae Experimentalis , ISSUE 3, 244–253

Research Article

First report of Pratylenchus vulnus associated with apple in Tunisia

The root-lesion nematode of the genus Pratylenchus Filipjev (1936) has a worldwide distribution and cause severe production constraints on numerous important crops. In 2013-14, during a survey of the apple nurseries and orchards in center of Tunisia (Kairouan, Zaghouan, Monastir and Kasserine), 70 different roots and soil samples were collected. The populations of root-lesion nematode were identified on the basis of their morphological and morphometric characters, and by molecular methods

Noura Chihani-Hammas, Lobna Hajji-Hedfi, Hajer Regaieg, Asma Larayedh, Ahmed Badiss, Yu Qing, Horrigue-Raouani Najet

Journal of Nematology , ISSUE 4, 579–586


Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism

, 1991) and populus in Henan province (Li and Yu, 1991), both descriptions lack detailed morphological or molecular data, and neither citrus nor populus are known to be the host of M. mali. Thus, the presence of M. mali in China remains questionable, as does the suitability of citrus and populus as hosts. Due to its wide host and broad distribution, M. mali was placed on the EPPO Alert List in 2014. Intragenomic polymorphisms have been found in various plant–parasitic nematode taxa, including genera

Jianfeng Gu, Yiwu Fang, Lele Liu

Journal of Nematology , 1–11


Taxonomy and Systematics of the Genus Makatinus Heyns, 1965 (Nematoda: Dorylaimida: Aporcelaimidae)

The taxonomy and the systematics of the genus Makatinus are discussed by means of the characterization of its morphological pattern and the first molecular (D2–D3 expansion segments of 28S rDNA) analysis of a representative of this taxon, Makatinus crassiformis from Costa Rica. The presence of two or more pairs of male ad-cloacal genital papillae is the most characteristic autapomorphy of the genus, but the status of its species on this concern differ among them. Both morphological and


Journal of Nematology , ISSUE 3, 245–253

Research Article

First Report of Carrot Cyst Nematode Heterodera carotae in Mexico: Morphological, Molecular Characterization, and Host Range Study

During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization

Ilia Mariana Escobar-Avila, Edgar Óliver López-Villegas, Sergei A. Subbotin, Alejandro Tovar-Soto

Journal of Nematology , ISSUE 2, 229–242


First report of molecular characterization and phylogeny of Trichuris fossor Hall, 1916 (Nematoda: Trichuridae)

, 1993). Descriptions have been based on morphology and host preference and Trichuris from geomyid hosts have never been sequenced (Eberhardt et al., 2019). The aim of this study was to serve as the first molecular report for T. fossor. The 18S rRNA gene was sequenced for four specimens identified putatively as T. fossor collected from separate Thomomys (western pocket gophers) species hosts. Materials and methods Thomomys bulbivorus (Richardson, 1829; Brandt, 1855) were salvaged from trappers

Malorri R. Hughes, Deborah A. Duffield, Dana K. Howe, Dee R. Denver

Journal of Nematology , 1–6


Molecular and Morphological Characterization of Aphelenchoides fuchsi sp. n. (Nematoda: Aphelenchoididae) Isolated from Pinus eldarica in Western Iran


Journal of Nematology , ISSUE 1, 34–42


First report of Meloidogyne javanica infecting Zinnia elegans in Ceará State, Brazil

were prepared according to Taylor and Netscher (1974). The determination of the esterase profile was made according to Carneiro and Almeida (2001), using 20 female. For molecular identification, the D2 to D3 region of 28S rDNA segment was amplified and sequenced using the primers D2A (5′-ACAAGTACCGTGAGGGAAAGTTG-3′) and D3B (5′-TCGGAAGGAACCAGCTACTA-3′) (De Ley et al., 1999) and ITS primers with VRAIN2F (5′-CTTTGTACACACCGCCCGTCGCT-3′) and VRAIN2R (5′-TTTCACTCGCCGTTACTAAGGGAATC-3′) (Vrain et al., 1992

Francisco Jorge Carlos Souza Junior, Mayara Castro Assunção

Journal of Nematology , 1–4


Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy

, V. fluvialis, and V. alginolyticus are reported as the causative agents of vibriosis in shrimp (de la Pena et al. 1993; Karunasagar et al. 1994; Lee et al. 1996; Lightner 1996; Austin and Zhang 2006; Chatterjee and Halder 2012). No molecular level study has so far been conducted to precisely identify the causal agent of vibrio sis in shrimp in Bangladesh and the required effective management for this worrisome disease. A number of antibiotics and chemotherapeutic agents have been used in shrimp


Polish Journal of Microbiology , ISSUE 4, 429–438


First report of potato rot nematode, Ditylenchus destructor Thorne, 1945 infecting Codonopsis pilosula in Gansu province, China

Chunhui Ni, Shuling Zhang, Huixia Li, Yonggang Liu, Wenhao Li, Xuefen Xu, Zhipeng Xu

Journal of Nematology , 1–2

Research Article

Occurrence of Sheraphelenchus sucus (Nematoda: Aphelenchoidinae) and Panagrellus sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

Two different nematode species were recovered from pomegranate decaying fruit in two localities in Southern Italy: the mycetophagus nematode Sheraphelenchus sucus and a bacterial feeder nematode belonging to the Panagrolaimidae (Rhabditida) family. Morphometrics of the Italian population of S. sucus closely resemble that of the type population, whereas some differences were found when compared with another population from Iran. Molecular characterization of the Italian S. sucus using the 18S


Journal of Nematology , ISSUE 4, 418–426


A New Species of the Rare Genus Anguillonema Fuchs, 1938 (Nematoda: Hexatylina, Sphaerularioidea) with Its Molecular Phylogenetic Study

Abstract: Anguillonema amolensis n. sp. is described and illustrated based on its morphological, morphometric, and molecular characters. The new species is characterized by its 575 to 820 mm long and wide body (body width at vulva = 30 to 59 mm), irregularly ventrally curved after fixation, five to six lines in lateral fields, 6.0 to 7.5 mm long stylet with small rounded knobs, pharynx lacking median bulb, pharyngo-intestinal junction anterior to nerve ring and excretory pore, females with


Journal of Nematology , ISSUE 3, 286–294


Morphological and molecular characterizations of Heterodera oryzae in Korea

. Species identification using morphological characters alone is very difficult and does not give correct results. A combination of both morphological and molecular approaches is very crucial for proper and accurate identification (Subbotin et al., 2015). The use of mitochondrial DNA cytochrome c oxidase subunit I (mtCOI) gene, ITS region, and D2-D3 expansion segments of 28S rDNA region sequences are trending today in molecular and phylogenetic studies (Hajibabaei et al., 2007; Subbotin et al., 2007

Rose Mwesige, Eun-Hwa Kim, Eun-Hyung Park, Hyoung-Rai Ko

Journal of Nematology , 1–12


Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

Globally, cyst nematodes species cause serious damage to a wide variety of economically important crops. The need for information on cyst-forming nematode species has been instrumental in stimulating growth of nematology worldwide. The cyst nematode group currently contains eight genera, with a total of 121 valid species (Handoo and Subbotin, 2018). The general morphology and molecular taxonomy and phylogeny of cyst nematodes have been given in detail in two recent review articles (Baldwin and

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Lynn K. Carta, Jamal Faghihi, Virginia Ferris

Journal of Nematology , 1–8

Research Article

Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran

Labrys filiformis n. sp., the second species of the rare genus Labrys, was recovered from natural forests of Gilan province and is described based upon morphological and molecular characters. The new species is characterized by its smooth cuticle under light microscopy, lateral field with two incisures forming a single plain band, lip region continuous with body contour, dorso-ventrally flattened and forming four poorly prominent lobes, having a dorso-ventrally narrower protuberant labial plate

Yousef Panahandeh, Joaquín Abolafia, Ebrahim Pourjam, Robin M. Giblin-Davis, Farahnaz Jahanshahi Afshar, Majid Pedram

Journal of Nematology , ISSUE 3, 343–354


First report of Xiphinema hunaniense Wang & Wu, 1992 (Nematoda: Longidoridae) in Vietnam

overlapping morphometrics of some species groups in the genus Xiphinema make quarantine regulations and protection methods more difficult. Therefore, accurate identification of Xiphinema species using integrate approach is strongly recommended to create a basis for plant pest management. In Vietnam, eight species of the genus Xiphinema have been reported, however, molecular identification are not available for most of them (Nguyen and Nguyen, 2000), and thus, a higher diversity of Xiphinema spp. is

Huu Tien Nguyen, Thi Duyen Nguyen, Thi Mai Linh Le, Quang Phap Trinh

Journal of Nematology , 1–4

Short Communication

Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of

Beata Zimowska, Ewa Dorota Zalewska, Ewa Dorota Król, Agnieszka Furmańczyk

Polish Journal of Microbiology , ISSUE 2, 281–285


First molecular characterization of an Iranian population of Schistonchus caprifici (Gasparrini, 1864) Cobb, 1927 (Rhabditida; Aphelenchoidea)

Goldansaz, 1998; Pakniyat and Taghizadeh, 2000; Namjoo et al., 2019) with no morphological and morphometric data. However, the morphological characters and morphometric data were provided for an Iranian population of the species by Kolaei et al. (2016) from Kerman. The genera Aprutides and Schistonchus were not characterized from Iran with molecular data, unlike the other previously mentioned genera. Based on a recovered population of S. caprifici from syconia of Ficus carica collected from a fig garden

Hadi Karimipour Fard, Hamid Zare

Journal of Nematology , 1–3


Molecular and morphological characterization of the alfalfa cyst nematode, Heterodera medicaginis, from Utah

report of the alfalfa cyst nematode in North America was reported in Kansas by Powers et al. (2019). Previously, unpublished records of this cyst species reproducing on alfalfa indicated the presence of this nematode in the Great Plains states of Kansas and Montana (Powers et al., 2019). The soil sample was collected from Delta, Utah (Millard county) and sent to University of Idaho for initial evaluation, after which it was submitted to USDA-ARS in Beltsville, MD for morphological and molecular

Zafar A. Handoo, Andrea M. Skantar, Saad L. Hafez, Mihail R. Kantor, Maria N. Hult, Stephen A. Rogers

Journal of Nematology , 1–4


Morphological and Molecular Characterization of Coslenchus paramaritus n. sp. and C. cancellatus (Cobb, 1925) Siddiqi, 1978 (Nematoda: Tylenchidae) from Iran

Province, southwestern Iran. Voucher specimens including 12 females were deposited in the collection of the Department of Plant Protection, College of Agriculture, University of Zanjan, Zanjan, Iran. Molecular phylogenetic status The amplification of D2-D3 expansion fragments of 28S rRNA gene sequences of C. paramaritus n. sp. and C. cancellatus yielded a single fragment of 736 (after editing 635) and 751 (after editing 642) bp, respectively. The D2-D3 expansion fragment of 28S rRNA alignment

Manouchehr Hosseinvand, Ali Eskandari, Reza Ghaderi

journal of nematology , 1–10


Description of Deladenus brevis n. sp. (Sphaerularioidea: Neotylenchidae) from Iran: a morphological and molecular phylogenetic study

has two entomoparasitic and free-living mycetophagous generations (Siddiqi, 2000). The list of the valid species under the genus is given by several authors (Siddiqi, 2000; Miraeiz et al., 2017; Yu et al., 2017; Morris et al., 2018). Almost all old species under the genus have been established based upon traditional approaches. On the contrary, several recently described species include molecular data (e.g., Yu et al., 2009, 2011, 2013, 2014; Morris et al., 2013; Tomar et al., 2015; Kanzaki et al

Fariba Heydari, Joaquín Abolafia, Majid Pedram

Journal of Nematology , 1–13


First report of Meloidogyne hapla on kiwifruit in South Africa

glycerin using De Grisse’s (1969) method. The characteristics perineal patterns of the second-stage juvenile (J2) were used to identify the test Meloidogyne species (Perry et al., 2009). Figure 1: Meloidogyne hapla Chitwood, 1949. (A) Roots of kiwi tree affected. (B) Mature female on the root. The molecular characterization followed the methods described in Álvarez-Ortega et al. (2019). The ribosomal ITS and LSU sequences were analyzed and aligned using the program BioEdit (Hall, 1999), aligned

Ebrahim Shokoohi, Phatu W. Mashela

Journal of Nematology , 1–5

Original Paper

Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters

Carotenoids are known to possess immense nutraceutical properties and microorganisms are continuously being explored as natural source for production of carotenoids. In this study, pigmented bacteria belonging to Flavobacteriaceae family were isolated using kanamycin-containing marine agar and identified using the molecular techniques and their phenotypic characteristics were studied along with their potential to produce carotenoids. Analysis of random amplification of polymorphic DNA (RAPD

Rama Sowmya, Nakkarike M. Sachindra

Polish Journal of Microbiology , ISSUE 1, 77–88



removed and, as wet gas, enters condenser (5). In this place gas is cooled down and water vapor condenses. Liquid is taken out in water knock out (6). After that gas is compressed in a compressor (7) to compensate losses in the unit and equalize pressure with raw gas. At the end it is reinjected to the stream coming from well [15]. Figure 3. Scheme of adsorption drying process (source: [15]) The most commonly used adsorbents in gas drying processes are silica gel (SiO2), molecular sieves (zeolites


Architecture, Civil Engineering, Environment , ISSUE 3, 73–83

Original Paper

Gas Gangrene of Different Origin Associated with Clostridium perfringens Type A in Three Patients Simultaneously Hospitalized in a Single Department of Orthopedics and Traumatology in Poland

The objective of the study was to perform a comparative analysis of phenotypic and genetic similarity, determination of resistance profiles, detection of toxin-encoding genes and molecular typing of Clostridium perfringens isolates originating from patients with gas gangrene. The study encompassed three patients with a clinical and microbiological diagnosis of gas gangrene who were hospitalized in one of the hospitals of the Kujawsko-Pomorskie province in the same period of time between 8th

Monika Brzychczy-Włoch, Dorota Ochońska, Anna Piotrowska, Małgorzata Bulanda

Polish Journal of Microbiology , ISSUE 4, 399–406


Morphological and molecular characterization of Epidorylaimus procerus sp. n. (Dorylaimida: Qudsianematidae) from Vietnam

). From the Russian species E. rivalis, it differs in being longer (2.16-2.46 vs 1.46-2.29 mm, n = 15), the lip region is less differentiated (weak vs deep constriction), and it has a longer odontostyle (32-35 vs 28-30 µm). Males of E. rivalis are as abundant as females, whereas they are unknown in E. procerus n. sp. and thus likely to be rare or absent. Evolutionary relationships, as derived from the analysis of D2-D3 28S-rRNA gene sequences, are presented in a molecular tree (Fig. 3). The new

Thi Anh Duong Nguyen, Reyes Peña-Santiago

Journal of Nematology , 1–8


Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

concluded that the overall HBsAg positivity rate for the TRNC is 1.2% (Arikan et al. 2016). There is a high demand for genotype information and investigation regarding HBV infected individuals. This importance rules to be informed regarding molecular and epidemiological specifications. As with the aim of this importance, we have aimed to analyze the dispersion of genotype/subgenotype/serotype together with pol gene mutations, which are related to NA therapy, and S gene mutations. Experimental


Polish Journal of Microbiology , ISSUE 4, 449–456


Description of Enchodorus yeatsi n. sp. (Dorylaimida, Nordiidae) from Southern Iran and Its Molecular Phylogenetic Study

Enchodorus yeatsi n. sp., a new species of the rare genus Enchodorus is described and illustrated based on its morphological and molecular characters. It was recovered from southern Iran. Females of the new species are characterized by having 1,511.3- to 1,792.5-mm long slender body, angular lip region having prominent papillae, 12- to 13-mm long odontostyle, double guiding ring, simple rod-like odontophore, didelphic–amphidelphic reproductive system, and 102- to 160-mm


Journal of Nematology , ISSUE 1, 21–26

Original Paper

Carbapenem-resistant Acinetobacter baumannii from Air and Patients of Intensive Care Units

To understand the molecular epidemiology and antibiotic resistance of air and clinical isolates of Acinetobacter baumannii, the intensive care unit settings of a hospital in Northern China were surveyed in 2014. Twenty non-duplicate A. baumannii isolates were obtained from patients and five isolates of airborne A. baumannii were obtained from the wards’ corridors. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homology relationships of


Polish Journal of Microbiology , ISSUE 3, 333–338


Morphological and molecular characterization of Acrobeloides saeedi Siddiqi, De Ley and Khan, 1992 (Rhabditida, Cephalobidae) from India and comments on its status

Rafiqius to include A. saeedi and other morphological related species as A. bodenheimeri (Steiner, 1936; Thorne, 1937). This newly proposed genus was differentiated from Acrobeloides (Cobb, 1924) according to the morphology of the lip region, having seta-like processes at labial primary axils. However, the creation of this new genus was considered unjustified by De Ley et al. (1999). Unfortunately, none of these studies provided molecular study. Figure 1: Acrobeloides saeedi (isolate KMW) (Siddiqi

Aasha Rana, Aashaq Hussain Bhat, Suman Bhargava, Ashok Kumar Chaubey, Joaquín Abolafia

Journal of Nematology , 1–21


Assessment of Globodera pallida RNA Extracted from Solanum Roots

results once the animals entered their hosts’ roots. We have compared several extraction procedures to ascertain whether a single protocol was capable of recovering high-quality, high-molecular-weight RNA from newly hatched J2 larva as well as from larva embedded in roots of both potatoes (Solanum tuberosum L. cv. Desiree) and a very distantly related species, Solanum sisymbriifolium. Although it was possible to recover large amounts of RNA from J2 larvae using Proteinase K


Journal of Nematology , ISSUE 1, 12–20

Research Article

Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus

study. Molecular characterization based on 18SSU rDNA sequencing performed to confirm the taxonomic position of this species and to documents the morphological data. Sequence alignment detects a percent of identity up to 88.0% with other Heteroxynematidae species. Phylogenetic analysis showed that the present recorded is a putative sister taxon to A. tetraptera recorded in a previous study. The SSU rDNA sequence has been deposited in the GenBank under the accession no. MG019400.

Rewaida Abdel-Gaber, Fathy Abdel-Ghaffar, Saleh Al Quraishy, Kareem Morsy, Rehab Saleh, Heinz Mehlhorn

Journal of Nematology , ISSUE 2, 117–132


Morpho-molecular characterization of Colombian and Brazilian populations of Rotylenchulus associated with Musa spp

., 2004; Jones et al., 2013). While numerous reports of Rotylenchulus associated with Musa spp. in Colombia exist (Zuñiga et al., 1979; Barriga and Cubillos, 1980; Curiel and Ospino, 2001; Gómez, 2001; Guzmán et al., 2012), detailed morphological, morphometric, and molecular data were not included. Thus, in Colombia, there is limited knowledge as to which species of Rotylenchulus are associated with Musa spp. which impedes the deployment of effective management strategies to control the species. To

Donald Riascos-Ortiz, Ana Teresa Mosquera-Espinosa, Francia Varón De Agudelo, Claudio Marcelo Gonçalves de Oliveira, Jaime Eduardo Muñoz-Flórez

Journal of Nematology , 1–13

Research Article

Delatylus andersoni n. gen., n. sp. (Nematoda: Neotylenchidae) Isolated from White Pine (Pinus monticola) Lumber from USA and Intercepted in Ningbo, China

opening large, and relative proximity of vulva to the anus. Detailed morphological and molecular characterization of the new genus/species is presented along with the comparison of the related genera.

Qing Yu, Maria Munawar, Jianfeng Gu, Weimin Ye

Journal of Nematology , ISSUE 1, 69–76


Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves

northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the


Journal of Nematology , ISSUE 1, 28–33


Description and molecular phylogeny of Mesocriconema abolafiai n. sp. (Nematoda: Criconematidae) from Iran

) analysis (Powers et al., 2016). Mesocriconema nebraskense (Olson et al., 2017) was described as a monosexual, cryptic species sympatrically distributed with its cryptic counterpart, M. curvatum (Raski, 1952; Loof and De Grisse, 1989; Olson et al., 2017). In this paper, we describe the new species M. abolafiai n. sp., based on morphological and molecular characteristics. Material and methods Nematode populations and morphological characterization The specimens were recovered from two localities in

Hossein Mirbabaei Karani, Ali Eskandari, Reza Ghaderi, Akbar Karegar

Journal of Nematology , 1–17


Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

., 2016). Thus, additional identification methods, particularly molecular-based identification, which requires a small amount of nucleic acid extracted from a single individual, will allow more accurate nematode identification, regardless of developmental stage (Handoo et al., 2016). The present study aimed to characterize R. similis using a combined morphological and morphometric approach and to confirm its identity using rDNA sequencing. Materials and methods Nematode populations We collected 314

Doreen M. Mgonja, Gladness E. Temu, Joseph C. Ndunguru, Magreth F. Mziray, Sylvester L. Lyantagaye, Nessie D. Luambano

Journal of Nematology , 1–8

Original Paper

Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland

using microarrays technology confirmed high effectiveness of this method in determining STEC virulotypes which provides data suitable for molecular risk assessment of the potential virulence of this bacteria.

Aleksandra Januszkiewicz, Waldemar Rastawicki

Polish Journal of Microbiology , ISSUE 3, 261–269

Research Article

Molecular Characterization and Phylogeny of Ditylenchus weischeri from Cirsium arvense in the Prairie Provinces of Canada

molecular analysis of many D. weischeri specimens from Canada is presented. Individuals from 41C. arvense or yellow pea grain samples with seeds of C. arvense from the Prairie Provinces were sequenced for the internal transcribed spacer (ITS rDNA), large subunit (LSU) D2D3 28S rDNA, partial segment of small subunit (SSU) 18S rDNA, and the heat shock protein Hsp90 gene. The analysis also included D. weischeri individuals from C. arvense from Russia and garlic with D. dipsaci from the Provinces of Ontario

Mehrdad Madani, Mario Tenuta

Journal of Nematology , ISSUE 2, 163–182


Description of a new dagger nematode, Xiphinema barooghii n. sp. (Nematoda: Longidoridae) and additional data on the three known species of the genus from northwest of Iran

the new species, according to the polytomous key of Loof and Luc (1990), are: A4, B3, C5a, D6, E5, F4, G3, H2, I3, J4, K2, L1. Based on the molecular and morphological similarities, the new species is closely related to X. aceri (Chizhov et al., 1986); X. granatum (Pedram, Pourjam, Palomares-Rius, Ghaemi, Cantalapiedra-Navarrete and Castillo, 2012); X. herakliense (Tzortzakakis et al., 2015); X. zagrosense (Ghaemi et al., 2012), and X. vuittenezi (Luc et al., 1964), but it can be separated using

Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Arezoo Naghavi

Journal of Nematology , 1–17

Research Article

Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

In a search for an entomopathogenic nematode to control cranberry insect pests, three Oscheius populations (Rhabditidae) were recovered through the Galleria-bait method from one sample taken in a wild cranberry marsh in Jackson County, Wisconsin, USA. Morphological studies with light microscopy and scanning electron microscopy, as well as molecular analyses of the near-full-length small subunit rDNA gene, D2/D3 expansion segments of the large subunit rDNA gene, internal transcribed spacer, and

Weimin Ye, Shane Foye, Ann E. MacGuidwin, Shawn Steffan

Journal of Nematology , ISSUE 1, 9–26


A role for the GDAP1 gene in the molecular pathogenesis of Charcot-Marie-Tooth disease

In 2002 a series of mutations in the GDAP1 gene were reported in patients suffering from Charcot-Marie-Tooth disease manifesting as early–onset, progressive distal-muscle wasting and weakness. The molecular etiology of Charcot-Marie-Tooth -GDAP1 disease has been elucidated but its pathogenesis remains unclear, especially given the seemingly contradictory function of the GDAP1 protein. Expression of GDAP1 is observed almost exclusively in neuronal cells, however, the GDAP1 protein is present in

Weronika Rzepnikowska, Andrzej Kochański

Acta Neurobiologiae Experimentalis , ISSUE 1, 1–13


Morphological and molecular characterization of Ektaphelenchoides pini (Massey, 1966) Baujard, 1984 (Aphelenchoididae; Ektaphelenchinae) from Iran, with morphological and taxonomic observations on some species

poinari (Aliramaji et al., 2014) revealed that a slightly projecting structure is present slightly anterior to the cloacal opening. This could be the single precloacal P1 papilla, or the paired papillae, similar to those in E. pini. The last pair is situated at 14.5 µm distance to the tail tip. The arrangement and number of papilla for this species are amended herein. Molecular characterization and phylogenetic relationships Partial SSU rDNA phylogeny To determine the phylogenetic relationships of

Fariba Heydari, Majid Pedram

Journal of Nematology , 1–12


First Report of the Spiral Nematode Rotylenchus incultus (Nematoda: Hoplolaimidae) from Cultivated Olive in Tunisia, with Additional Molecular Data on Rotylenchus eximius

al. (2003). Moderate-to-low soil populations of these spiral nematodes were detected (5.5–11.5, 1.5–5.0 individuals/500 cm3 of soil, respectively). This prompted us to undertake a detailed morphological and molecular comparative study with previous reported data. Morphological and molecular analyses of females identified these species as Rotylenchus eximius Siddiqi, 1964, and Rotylenchus incultus Sher, 1965. The morphology of R. eximius females (five specimens studied) was


Journal of Nematology , ISSUE 3, 136–138


Description of a New Anguinid Nematode, Nothotylenchus phoenixae n. sp. (Nematoda: Anguinidae) Associated with Palm Date Trees and Its Phylogenetic Relations within the Family Anguinidae

sac well developed, 15 (14 to 17) mm long, female tail elongate-conoid with pointed terminus; and male with adanal bursa and spicules 21 to 22 mm long (n = 2). The new species comes close in morphology and morphometrics to five known species of the genus, namely N. affinis, N. hexaglyphus, N. persicus, N. taylori, and N. uniformis. Molecular analyses of the partial 18S, D2/D3 expansion segments of the partial 28S and internal transcribed spacer (ITS) revealed this as a new species. The sequences


Journal of Nematology , ISSUE 3, 268–275

Research Article

Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination

morphometric data ranges. The morphological features and morphometrics of the second studied species, A. helicus, agreed well with the data given for the type population. However, detailed study of fresh females revealed it has three drop-shaped stylet knobs and long PUS, making it typologically similar to the genus Robustodorus, meriting its taxonomic revision, i.e., transferring to it. In molecular phylogenetic analyses using partial small and large subunit ribosomal RNA gene (SSU and LSU rDNA) sequences

Farzad Aliramaji, Ebrahim Pourjam, Sergio Álvarez-Ortega, Farahnaz Jahanshahi Afshar, Majid Pedram

Journal of Nematology , ISSUE 3, 437–452

Research Article

First Reports, Morphological, and Molecular Characterization of Longidorus caespiticola and Longidorus poessneckensis (Nematoda: Longidoridae) from Ukraine

from L. poessneckensis, showed 99% to other sequences of L. poessneckensis deposited in GenBank from Slovakia and Czech Republic. Morphology, morphometry, and molecular data obtained from these samples were consistent with L. caespiticola and L. poessneckensis identification. To our knowledge, these are the first reports of L. caespiticola and L. poessneckensis in Ukraine, extending the geographical distribution of these species.


Journal of Nematology , ISSUE 4, 396–402

Research Article

Molecular Identification of Entomopathogenic Nematode Isolates from the Philippines and their Biological Control Potential Against Lepidopteran Pests of Corn

most susceptible to H. indica PBCB (21.90 hr) and S. abbasi (21.18 hr). Our results suggest that H. indica PBCB and S. abbasi MBLB are good candidates as biological control agents against these insect pests of corn. Moreover, O. furnacalis as alternative bait for EPN was discussed. To date, this is the most extensive research on Philippine EPN, comprised of wide sampling coverage, molecular identification and bioefficacy assays.

Barbara L. Caoili, Romnick A. Latina, Regina Faye C. Sandoval, Joey I. Orajay

Journal of Nematology , ISSUE 2, 99–110

Research Article

Xiphinema japonicum n. sp. (Nematoda: Longidorinae) from the Rhizosphere of Japanese Podocarpus macrophyllus (Thunb.), a Cryptic Species Related to Xiphinema bakeri Williams, 1961

bakeri, herein considered as its cryptic species by the nature of high morphological similarity, but with significant differences in DNA sequences in nearly full length 18S, ITS1, 28S D2/D3, and cytochrome c oxidase subunit 1 sequences. The second morphologically close species is Xiphinema setariae from which the new species could be separated based on morphological and molecular characters. Morphological comparisons with phylogenetically related species are also discussed.


Journal of Nematology , ISSUE 4, 404–417


Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq

likely inflated as a result of false positives caused by the morphologically indistinguishable, nonpathogenic E. dispar/moshkovskii, and/or polymorphic nuclear leukocytes and macrophages with similar morphology in the stool samples (Walsh 1986; Tanyuksel and Petri 2003). New methods have been developed that are better in distinguishing between the pathogenic E. histolytica and nonpathogenic amoebae in the stool sample. Emerging molecular-based techniques, such as polymerase chain reaction (PCR), have


Polish Journal of Microbiology , ISSUE 3, 263–272

Research Article

First Report of Bitylenchus hispaniensis, Pratylenchoides alkani, and Helicotylenchus vulgaris in Association with Cultivated and Wild Olives in Crete, Greece and Molecular Identification of Helicotylenchus microlobus and Merlinius brevidens

alkani is herein reported for the second time in the Mediterranean area and for the first time in association with olive. Two further populations of H. microlobus and H. vulgaris, from walnut and goji berry from Greece, were identified. Molecular data for all of these nematode species are provided, resulting in the first integrative identification of these Greek populations.

Emmanuel A. Tzortzakakis, Carolina Cantalapiedra-Navarrete, Maria Kormpi, Maria S. Lazanaki, Pablo Castillo, Antonio Archidona-Yuste

Journal of Nematology , ISSUE 3, 413–418


Molecular and Morphological Characterization of Xiphinema chambersi Population from Live Oak in Jekyll Island, Georgia, with Comments on Morphometric Variations

A population of Xiphinema chambersi from the root zone around live oak (Quercus virginiana Mill.) trees on Jekyll Island, GA, is described using both morphological and molecular tools and compared with descriptions of type specimens. Initially, because of a few morphological differences, this nematode was thought to represent an undescribed species. However, on further examination, the morphometrics of the nematodes from live oak tend to agree with most of the morphometrics in the


Journal of Nematology , ISSUE 1, 20–27

Original Paper

The Heavy-Metal Resistance Determinant of Newly Isolated Bacterium from a Nickel-Contaminated Soil in Southwest Slovakia

[CP001068] with 99 and 100% similarities, respectively. In addition, the whole nccA-like heavy-metal resistance gene sequence (3,192 bp), marked as MR-CH-I2-nccA [KR476581], was obtained and on the base of phylogenetic analysis its assignment was confirmed to MULTISPECIES: cation efflux system protein CzcA [WP_004635342] from Burkholderiaceae with 98% similarity. Furthermore, although the bacterium carried one high molecular plasmid of about 50 kb in size, nccA-like gene was not located on this plasmid


Polish Journal of Microbiology , ISSUE 2, 191–201


First report of root-knot nematode, Meloidogyne arenaria, on lavender in Turkey

. Galled roots were collected from seedlings and examined with a stereo-binocular microscope. The galls were symptoms caused by root-knot nematodes (Fig. 1). Egg masses were separately collected from roots of L. angustifolia cultivars, Raya, Hemus, Sevtopolis, Hebar and Yubileina using a small needle. For molecular identification, DNA was extracted from egg masses using the High Pure PCR Template Preparation Kit (Roche). Then, DNA samples were screened by species-specific primers belonging to the most

Tevfik Özalp, Gonca Könül, Önder Ayyıldız, Adnan Tülek, Zübeyir Devran

Journal of Nematology , 1–3

Research Article

Description of Xiphinema parachambersi sp. n. (Nematoda: Longidoridae) from Imported Ornamental Plants in Japan with a Key to Xiphinema Species in Group 1

Munawar Maria, Weimin Ye, Qing Yu, Jianfeng Gu

Journal of Nematology , ISSUE 3, 369–386

Original Paper

Non-invasive Diagnostic of Helicobacter pylori in Stools by Nested-qPCR

María I. Taborda, Gisela Aquea, Yenny Nilo, Karla Salvatierra, Nicolás López, Sergio López, Gustavo Bresky, Juan A. Madariaga, Vittorio Zaffiri, Sergio Häberle, Giuliano Bernal

Polish Journal of Microbiology , ISSUE 1, 11–18


Plant-parasitic nematodes associated with sugarcane in Kilimanjaro, Tanzania

Meloidogyne, Pratylenchus, Tylenchorhynchus, Rotylenchulus, and Helicotylenchus. This study was aimed at studying the diversity of PPN associated with sugarcane, with focus on the sugarcane plantation sites of TPC Limited in Kilimanjaro region. The combination of morphological and molecular analyses revealed the presence of several genera of PPN in the soil and root samples collected from 12 different sugarcane fields. Molecular and morphological characterizations are provided for the most important PPN

Phougeishangbam Rolish Singh, Beatrice E. Kashando, Marjolein Couvreur, Gerrit Karssen, Wim Bert

Journal of Nematology , 1–17

Short Communication

Genetic Characterization of Human Enteroviruses Associated with Hand, Foot and Mouth Diseases in Poland, 2013–2016

The objective of the present study was to describe the molecular characteristics of enteroviruses associated with hand, food, and mouth disease (HFMD) in Poland. Clinical material from HFMD cases, that occurred during 2013–2016 were examined. It has been showed that coxsackievirus A6 (CVA6), CVA10 and CVA16 were circulating in the country. Phylogenetic analysis showed that Polish CVA6 strains were divided into two distinct clusters suggesting two independent introductions. This is the

Magdalena Wieczorek, Agnieszka Ciąćka, Arleta Krzysztoszek, Agnieszka Figas, Leszek Szenborn

Polish Journal of Microbiology , ISSUE 3, 405–409


Description of Deladenus gilanica n. sp. (Hexatylina: Neotylenchidae) isolated from wood of black pine in Northern Iran

was recovered from a wood sample of a dead pine tree (Pinus niger L.) in Siahkal region, Guilan Province, Northern Iran. The observations revealed that this population appeared to be morphologically and morphometrically distinct from any existing Deladenus species and it is herein described as D. gilanica n. sp. The objectives of the present study were: (i) to provide an accurate description of the new species by an integrative approach to morphological and molecular characterization using the

Parisa Jalalinasab, Mehrab Esmaeili, Weimin Ye, Ramin Heydari

Journal of Nematology , 1–10


Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

group and insectivora group. Diagnostic characters of both the groups are unique; species under the insectivora group are characterized by leptoderan bursa, crochet needle-shaped spicules and normal rectum, whereas the dolichura group has peloderan bursa, probe head spicule tips and expandable rectum. On the basis of morphological and molecular studies, Sudhaus (2011) characterized the family Rhabditidae by generalized body plan into three groups, Pleiorhabditis, Synrhabditis, and Anarhabditis. He

Puneet Kumar, Wajih Jamal, Vishal S. Somvanshi, Khushbu Chauhan, Sabia Mumtaz

Journal of Nematology , 1–11

Short Communication

Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods

Agnieszka Figas, Magdalena Wieczorek, Bogumiła Litwińska, Włodzimierz Gut

Polish Journal of Microbiology , ISSUE 4, 479–483


c-Fos and neuronal plasticity: the aftermath of Kaczmarek’s theory

The development of molecular biology methods in the early 1980s led to a better understanding of the role of transcription factors in mammalian cells. The discovery that some transcription factors are critically important for cells to switch between different functional states was fundamental for modern molecular neurobiology. In the 1980s Leszek Kaczmarek proposed that, analogically to the cell cycle or to cell differentiation, long-term synaptic plasticity, learning, and memory should also

Jacek Jaworski, Katarzyna Kalita, Ewelina Knapska

Acta Neurobiologiae Experimentalis , ISSUE 4, 287–296


Paurodontella parapitica n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Kermanshah Province, Western Iran


Journal of Nematology , ISSUE 2, 109–115


Evidence for Infections by the Same Strain of Beta 2-toxigenic Clostridium perfringens Type A Acquired in One Hospital Ward

identify the differences and similarities among bacterial strains from every patient. The use of molecular methods allows for fast and precise identification of the bacteria isolated. The objective of this study is a comparative phenotypic and genetic analysis of C. perfringens strains isolated from two patients hospitalized at the same time in 2017 in the Provincial Specialist Hospital in Włocławek (Kujawsko-Pomorskie Province) who developed soft tissue infections. Trying to explain recurring cases of


Polish Journal of Microbiology , ISSUE 3, 323–329


New data on known species of Hirschmanniella and Pratylenchus (Rhabditida, Pratylenchidae) from Iran and South Africa

(Majd Taheri et al., 2013). Those species have been studied by morphological characters except for two unknowns which have been studied by morphological and molecular DNA barcoding using 28S rDNA (Majd Taheri et al., 2013). Root-lesion nematodes, Pratylenchus (Filipjev, 1936), are after root-knot and cyst nematodes listed as the third economically most important genus that adversely affects crop production worldwide (Castillo and Vovlas, 2007; Jones et al., 2013). Pratylenchus hippeastri, the

Ebrahim Shokoohi, Joaquín Abolafia, Phatu William Mashela, Nafiseh Divsalar

Journal of Nematology , 1–26


Data on Some Species of the Genus Coslenchus Siddiqi, 1978 (Rhabditida, Tylenchidae) from Iran

Data on five known species of the genus Coslenchus are provided. Morphological and morphometric data are given for all studied species. Three of the recovered species were also characterized by molecular phylogenetic data. The species C. leiocephalus was studied for the first time since its original description. Males of the species, C. franklinae and C. oligogyrus were described for the first time and the species C. oligogyrus was reported from Iran for the first time. In molecular


Journal of Nematology , ISSUE 4, 268–279

Original Paper

Aspergillus penicillioides Speg. Implicated in Keratomycosis

Abstract The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis


Polish Journal of Microbiology , ISSUE 4, 407–416


Characterization of a population of Pelodera strongyloides (Nematoda: Rhabditidae) associated with the beetle Lucanus ibericus (Coleoptera: Lucanidae) from Georgia

molecular analysis was carried out at the Institute for Sustainable Plant Protection in Bari, Italy. Nematode pathogenicity Nematode pathogenicity of P. strongyloides was evaluated in comparison to Steinernema carpocapsae (Weiser, 1955) Wouts, Mráček, Gerdin, and Bedding, 1982, using last instar larvae of G. mellonella. Four Petri plates with 15 G. mellonella (last instar lavae) each were used for the tests. A distilled water suspension of 3 ml containing about 100 specimens/1 insect larva was

O. Gorgadze, A. Troccoli, E. Fanelli, E. Tarasco, F. De Luca

Journal of Nematology , 1–12


Predominance of Lactobacillus plantarum Strains in Peruvian Amazonian Fruits

, the API 50CHL (Biomerieux, Marcy l’etoile, France) kit, which tests for 49 carbohydrates and esculin. Other systems designed for Gram-positive or Gram-negative bacteria have been applied to LAB identification, such as the Biolog system, which includes the fermentation of 96 carbohydrates (Moraes et al. 2013). On the other hand, the development of molecular techniques has allowed more accurate identification of LAB. The wide method used for this purpose is based on ribosomal gene sequencing or


Polish Journal of Microbiology , ISSUE 1, 127–137


Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey

infects the cortex of the plant roots and causes necrosis of roots, reduced root system, yellowed leaves, whole plant dwarfing, and plant dead (CABI, 2019). It reproduces on a wide range of plant species from strawberry to walnut on many field crops and forest trees (Pinochet et al., 1992). The genus Pratylenchus spp. has high intra specific variation. Additionally, low number of diagnostic features depending on the reproductive strategy of the species requires molecular identification (Castillo and

Mehmet Sait Karaca, Elif Yavuzaslanoglu, Gul Imriz, Ozlem Ates Sonmezoglu

Journal of Nematology , 1–4


Discopersicus n. gen., a New Member of the Family Tylenchidae Örley, 1880 with Detailed SEM Study on Two Known Species of the Genus Discotylenchus Siddiqi, 1980 (Nematoda; Tylenchidae) from Iran

Discopersicus iranicus n. gen., n. comb., previously described from Iran as a new species under the genus Discotylenchus, is illustrated using light microscope and scanning electron microscope (SEM) observations and further studied using molecular characters. SEM studies revealed the newly proposed genus has oblique amphidial apertures on the lateral sides of the lip region. SEM images are also provided for two species of Discotylenchus, namely D. discretus and D. brevicaudatus, as the first


Journal of Nematology , ISSUE 3, 214–221

Original Paper

Molecular Study of Indigenous Bacterial Community Composition on Exposure to Soil Arsenic Concentration Gradient

Semanti Basu, Tanima Paul, Priya Yadav, Abhijit Debnath, Keka Sarkar

Polish Journal of Microbiology , ISSUE 2, 209–221

Original Paper

Biochemical and Molecular Characterization of a Native Haloalkalophilic Tolerant Strain from the Texcoco Lake


Polish Journal of Microbiology , ISSUE 3, 377–382


First report of Mesocriconema sphaerocephalum (Taylor, 1936) Loof, 1989 associated with carrot (Daucus carota subsp. Stativus) in Vietnam

Zeiss Axio Lab.A1 light microscope. Measurements and pictures were taken using a ZEN lite software on ZEISS Axiocam ERc5s digital camera (Nguyen et al., 2017). For molecular studies, Primers D2A (5′-ACAAGTACCGTGGGGAAA GTTG-3′) and D3B (5′-TCGGAAGGAACCAGCTAC TA-3′) were used to amplify D2D3 of 28S rDNA region (Nguyen et al., 2017). Obtained sequence was used for a Blast search in GenBank (Altschul et al., 1997). The data set was analyzed using maximum likelihood (ML) method in MEGA 6 program with

Thi Duyen Nguyen, Huu Tien Nguyen, Thi Mai Linh Le, Thi Tuyet Thu Tran, Neriza Nobleza, Quang Phap Trinh

Journal of Nematology , 1–4


First report of Meloidogyne enterolobii infecting Japanese blue berry tree (Elaeocarpus decipiens) in Florida, USA

) were collected directly from the rhizosphere of E. decipiens. These samples were designated with the internal FDACS-DPI sample identifier N19-1242. Galls were observed on secondary and tertiary roots (Fig. 1). Females were found inside of the galls, and egg masses were outside. Fig. 1 Roots of Elaeocarpus decipiens F.B.Forbes & Hemsl. showing galls induced by Meloidogyne enterolobii (Yang and Eisenback, 1983). Nematode species were identified using molecular and isozyme analyses, body length

M. R. Moore, J. A. Brito, S. Qiu, C. G. Roberts, L. A. Combee

Journal of Nematology , 1–3


Description of Aphelenchoides macrospica n. sp. (Nematoda:Aphelenchoididae) from Northwestern Iran


Journal of Nematology , ISSUE 1, 67–76


New Hosts and Records in Portugal for the Root-Knot Nematode Meloidogyne luci

to identify the species. The esterase phenotype from young egg-laying females protein extracts of the three isolates exhibited three bands of esterase activity (Rm: 0.38; 0.43; 0.48) (Fig. 1) corresponding to the M. luci L3 phenotype (Maleita et al., 2018). A M. javanica isolate (J3, Rm: 0.38; 0.45; 0.49) was used as reference isolate to determine the relative position of M. luci esterase bands (Fig. 1). Molecular identification was performed by amplification and sequencing of the cytochrome

Duarte Santos, António Correia, Isabel Abrantes, Carla Maleita

Journal of Nematology , 1–4


Description of Laimaphelenchus sinensis n. sp. (Nematoda: Aphelenchoididae) from declining Chinese pine, Pinus tabuliformis in Beijing, China

Newcombe, 2013), although their possible association with oak decline syndrome was suggested by Pedram et al. (2018). During the present study, a population of Laimaphelenchus species was isolated from declining Chinese pine, Pinus tabuliformis Carrière, in Beijing, China, in November, 2018. The population was examined carefully and preliminary studies reveal the status of this species as a new species. Therefore, the objectives of the study are: to provide morphological and molecular characterization

Jianfeng Gu, Munawar Maria, Yiwu Fang, Lele Liu, Yong Bian, Xianfeng Chen

Journal of Nematology , 1–1

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