original-paper | 18-February-2020
-clamp. The PCR products were purified and sent to the Beijing Genomics Institute (Beijing, China) for clone sequencing. Sequence information was analyzed by aligning the results with sequences in GenBank using BLAST (Bligh et al. 1959; Wang et al. 2018).
PLFA extraction and analysis. PLFAs were extracted using a modified Bligh and Dyer procedure (Jiang et al. 2018). 5 g of Daqu and 30 ml of the Bligh and Dyer extraction buffer were placed into a 50 ml centrifuge tube. The samples were homogenized
Polish Journal of Microbiology, Volume 69 , ISSUE 1, 27–37
original-paper | 12-May-2020
fatty acid (PLFA) is one of the most popular techniques for analyzing of total biomass in a sample (Green and Scow 2000; Zheng et al. 2013; Ding et al. 2015). Therefore, pyrosequencing and PLFAs were employed to assess the structure of prokaryotic communities within the FPM and to reveal the changes within these communities with age during the FPM maturation. In addition, the relationship between environmental factor variables and prokaryotic community structure and diversity in the FPM was revealed
Polish Journal of Microbiology, Volume 69 , ISSUE 2, 151–164