research-article | 30-November-2020
the present study, two populations of the family Tylenchidae were recovered from natural forests of Golestan province, north Iran. The first species appeared as being a new member of Filenchus, and the second species belonged to Malenchus gilanensis (Jalalinasab et al., 2019). Thus, the present study aims to (i) characterize the new species using both traditional and molecular criteria, and (ii) update the phylogeny of Malenchus by including M. gilanensis in the LSU tree.
Materials and methods
Parnaz Mortazavi,
Fariba Heydari,
Joaquín Abolafia,
Pablo Castillo,
Majid Pedram
Journal of Nematology, Volume 53 , 1–14
research-article | 24-April-2020
overlapping ranges of measurements (Callejón et al., 2015, 2016; Vejl et al., 2017) and host preference may be more variable than previously thought (Doležalová et al., 2015). Approximately 24 species been reported from rodents, many of which exhibit comparable morphological patterns (Robles et al., 2014; Doležalová et al., 2015; Eberhardt et al., 2019).
Understanding the diversity and phylogeny of whipworms is important; false classification limits our acuity of biogeography and conceals the zoonotic
Malorri R. Hughes,
Deborah A. Duffield,
Dana K. Howe,
Dee R. Denver
Journal of Nematology, Volume 52 , 1–6
research-article | 24-April-2020
classification from species level up to family level (Andrássy, 2007; Brzeski, 1998; Qing and Bert, 2019; Siddiqi, 2000).
With the improved availability of genetic sequencing, molecular sequences in species diagnosis and phylogeny analysis have consolidated them as one of the most powerful tools in current taxonomy. Among marker genes, the ribosomal RNA (rRNA) genes are being used as the standard barcode for almost all animals and successfully resolved several groups in Nematoda (Bert et al., 2008; Holterman
Mengxin Bai,
Xue Qing,
Kaikai Qiao,
Xulan Ning,
Shun Xiao,
Xi Cheng,
Guokun Liu
Journal of Nematology, Volume 52 , 1–12
research-article | 30-November-2020
Farhad Saeidi Naeini,
Zahra Majd Taheri
Journal of Nematology, Volume 53 , 1–7
research-article | 30-November-2020
The taxonomy and phylogeny of tylenchids have already been studied and revised by several authors (Bert et al., 2008; De Ley and Blaxter, 2002; Holterman et al., 2009; Panahandeh et al., 2019; Sturhan, 2012; Subbotin et al., 2006). Following the molecular phylogenetic study of De Ley and Blaxter (2002), the order Tylenchida Thorne, 1949 sensu Siddiqi (2000), together with the former order Aphelenchida Siddiqi, 1980, formed the infraorder Tylenchomorpha De Ley and Blaxter, 2002 within the order
Mohammad Amiri Bonab,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 53 , 1–14
Article | 03-December-2017
Hemicycliophora. The 18S rDNA dataset was insufficient for distinguishing genus boundaries between Criconema, Ogma, and Crossonema. The relationships depicted by the 18S rDNA phylogeny suggest that key morphological characters used in the classification of Criconematidae are not homologous.
THOMAS POWERS,
TIMOTHY HARRIS,
REBECCA HIGGINS,
PETER MULLIN,
KIRSTEN POWERS
Journal of Nematology, Volume 49 , ISSUE 3, 236–244
research-article | 25-May-2020
Hossein Mirbabaei Karani,
Ali Eskandari,
Reza Ghaderi,
Akbar Karegar
Journal of Nematology, Volume 52 , 1–17
Research Article | 03-September-2018
Mehrdad Madani,
Mario Tenuta
Journal of Nematology, Volume 50 , ISSUE 2, 163–182
research-article | 30-November-2019
and Renčo (2007; Slovakia), Carrión and Desgarennes (2012; Mexico), Gagarin (2018; Vietnam), and Shurkurovich and Akvarovna (2018; Uzbekistan).
Phylogeny and systematics of Eucephalobus
Diagnosis (emended)
Cephalobidae, Cephalobinae: they are small nematodes, 0.36 to 1.00 mm long, cuticle bearing transverse striation, lateral field with three longitudinal incisures, lip region continuous with the adjacent body and consisting of conoid lips, primary and secondary axils showing similar
Joaquín Abolafia,
Reyes Peña-Santiago
Journal of Nematology, Volume 52 , 1–20
research-article | 30-November-2020
the host cadaver recovers infectivity and movement (Shapiro and Glazer, 1996; Shapiro and Lewis, 1999; Shapiro-Ilan et al., 2003).
This study uses a phylogenetic framework to assess how responsiveness to macerate from an EPN-infected insect, which contains both general host cues and species-specific pheromones, varies across four EPN species with increasing phylogenetic distance from a fifth “reference” species, Heterorhabditis bacteriophora. We used a 2016 phylogeny (Spiridonov and Subbotin, 2016
Hilal Erdogan,
Glen Stevens,
Asa Stevens,
David Shapiro-Ilan,
Fatma Kaplan,
Hans Alborn,
Edwin Lewis
Journal of Nematology, Volume 53 , 1–9
Article | 21-July-2017
as the closely related Ottolenchus were given based on a combination of morphology and recent molecular data, and their phylogeny were analyzed in a context of Tylenchidae. Furthermore, we test different fungi and moss as a food resource of Malenchus and their feeding behavior is also discussed.
XUE QING,
WIM BERT
Journal of Nematology, Volume 49 , ISSUE 2, 189–206
research-article | 11-March-2021
. However, the biology, morphology, and phylogeny of this species have not been well elucidated. Both species were detected in galleries of Scolytus multistriatus (Marsham, 1802), a bark beetle that is known to transmit Dutch elm disease (DED). Their dauer juveniles were also regularly collected in beetle adults, under elytra.
The objectives of this paper were to (i) describe and characterize morphologically all developmental stages of R. ulmi, including the dauer juvenile stage, (ii) describe R. ulmi’s
Alexander Y. Ryss,
Kristina S. Polyanina,
Sergio Álvarez-Ortega,
Sergei A. Subbotin
Journal of Nematology, Volume 53 , 1–25
Research Article | 03-December-2018
The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new
L. K. Carta,
S. Li
Journal of Nematology, Volume 50 , ISSUE 4, 533–542
research-article | 30-November-2018
clustering (PP = 81%) with two isolates of an unknown species of Coslenchus (JQ005005 and JQ005006), but separated from the sequences of other Coslenchus species.
Figure 5:
Figure 5: The 50% majority rule consensus trees from Bayesian analysis generated from the D2–D3 expansion fragments of 28 S rRNA gene dataset under GTR+I+G model. Posterior probabilities for BI analysis more than 50% are given for appropriate clades. New sequences are indicated in bold.
Discussion
In our phylogeny tree, the
Manouchehr Hosseinvand,
Ali Eskandari,
Reza Ghaderi
journal of nematology, Volume 51 , 1–10
research-article | 30-November-2019
allowance for gamma distribution of rate variation (GTR+Γ) in RAxML v8 (Stamatakis, 2014). The phylogeny of Helicotylenchus was inferred using MrBayes v3.2.6 (Ronquist et al., 2012) with the GTR + Γ model. Two independent Metropolis-coupled Markov chain Monte Carlo (MCMCMC) searches were performed for 2 million generations sampling every 2,000 steps. Convergence was assessed using Tracer v1.5 (burn-in = 20% of the samples), and by examining the average standard deviation of split frequencies among
Donald Riascos-Ortiz,
Ana Teresa Mosquera-Espinosa,
Francia Varón De Agudelo,
Claudio Marcelo Gonçalves de Oliveira,
Jaime Eduardo Muñoz-Florez
Journal of Nematology, Volume 52 , 1–19
Research Article | 26-September-2018
rRNA gene, D2–D3 expansion domains of the 28S rDNA, the ITS region, and the partial mitochondrial COI were carried out. Sequences of the 18S rRNA gene, the D2–D3 domains, and the ITS were analyzed using several methods for inferring phylogeny to reconstruct the relationships among Sheraphelenchus and Bursaphelenchus species. The bacterial feeder Panagrellus sp. was characterized at the molecular level only. The D2–D3 expansion domains and ITS sequences of this Italian panagrolaimid were determined
ELENA FANELLI,
ALBERTO TROCCOLI,
NICOLA VOVLAS,
GIANLUCA SCARCIA,
ANNAMARIA MINCUZZI,
SIMONA M. SANZANI,
ANTONIO IPPOLITO,
FRANCESCA DE LUCA
Journal of Nematology, Volume 49 , ISSUE 4, 418–426
Original Paper | 28-December-2016
all bacteria isolated from dolomite and limestone rocks were divided into 4 bacterial groups, including Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. For these two kinds of rocks, Proteobacteria was the first dominant group, and Gammaproteobacteria occupied the greatest proportion which might be closely related to Pseudomonas in phylogeny to be the most dominant genera after isolation. Actinobacteria and Bacillus bacteria were also widespread in these two kinds of rock environments
Yuan Tang,
Jian-Zhong Cheng,
Bin Lian
Polish Journal of Microbiology, Volume 65 , ISSUE 4, 413–423
research-article | 30-November-2019
Greicy Andrea Sarria,
Donald Riascos-Ortiz,
Hector Camilo Medina,
Yuri Mestizo,
Gerardo Lizarazo,
Francia Varón De Agudelo
Journal of Nematology, Volume 52 , 1–12
Research Article | 17-October-2018
Victor Phani,
Satyapal Bishnoi,
Amita Sharma,
Keith G. Davies,
Uma Rao
Journal of Nematology, Volume 50 , ISSUE 3, 387–398
Original Paper | 04-September-2018
MARISELA YADIRA SOTO-PADILLA,
PABLO GORTÁRES-MOROYOQUI,
LUIS ALBERTO CIRA-CHÁVEZ,
MARÍA ISABEL ESTRADA-ALVARADO
Polish Journal of Microbiology, Volume 67 , ISSUE 3, 377–382
research-article | 18-March-2020
with the SSU rDNA of S. hyrcania (KT354242). The identity value with other sequences was all less than 91%.
A number of 38 ektaphelenchid+seinurid, a Noctuidonema sp., an Anomyctus sp., an Peraphelenchus sp., two aphelenchid and three rhabditid SSU sequences were selected for the SSU phylogeny. A number of 52 ektaphelenchid + seinurid, a Noctuidonema sp., two aphelenchid and three rhabditid LSU D2-D3 sequences were selected for the LSU phylogeny. The selection of the sequences for both analyses was
Jianfeng Gu,
Munawar Maria,
Lele Liu,
Majid Pedram
Journal of Nematology, Volume 52 , 1–11
research-article | 13-April-2020
Hadi Karimipour Fard,
Hamid Zare
Journal of Nematology, Volume 52 , 1–3
research-article | 26-March-2021
Ebrahim Shokoohi
Journal of Nematology, Volume 53 , 1–9
research-article | 30-November-2020
) that were found in previous studies in Yunnan province were not detected in our study.
The phylogenetic tree based on Neighbor-Joining proposed a reasonably good resolution among the distal clades. Our wide-ranging analyses of the phylogeny constructed on the ITS region provides a novel vision into the interactions between groups and species in Steinernematids. The results support the same evolutionary lines previously found by Stock et al. (2001) that were based on the partial sequence of the 28S
Bingjiao Sun,
Xiuqing Zhang,
Li Song,
Lixin Zheng,
Xianqin Wei,
Xinghui Gu,
Yonghe Cui,
Bin Hu,
Toyoshi Yoshiga,
Mahfouz M. Abd-Elgawad,
Weibin Ruan
Journal of Nematology, Volume 53 , 1–17
research-article | 30-November-2018
accession numbers given in LSU and ITS1 trees.
Phylogenetic analyses
The newly generated sequences were compared with the available sequences in the GenBank database using the basic local alignment search tool (BLAST) (https:// blast.ncbi.nlm.nih.gov/Blast.cgi). For LSU phylogeny, several available sequences of the genus were retrieved from the database (a large and a smaller pruned LSU datasets were prepared). The currently available ITS1 sequences of Longidorus spp. were retrieved for the ITS1
Mazdosht Giti,
Leila Kashi,
Majid Pedram
journal of nematology, Volume 51 , 1–11
research-article | 03-June-2019
L. K. Carta,
S. Li
Journal of Nematology, Volume 51 , 1–8
Article | 21-July-2017
LYNN K. CARTA,
SHIGUANG LI,
ANDREA M. SKANTAR,
GEORGE NEWCOMBE
Journal of Nematology, Volume 48 , ISSUE 1, 28–33
Article | 24-July-2017
MAJID PEDRAM
Journal of Nematology, Volume 49 , ISSUE 2, 223–230
research-article | 30-November-2020
Wenhao Li,
Huixia Li,
Chunhui Ni,
Mingming Shi,
Xuejuan Wei,
Yonggang Liu,
Yiwen Zhang,
Deliang Peng
Journal of Nematology, Volume 53 , 1–15
research-article | 30-November-2020
described in Hooper, 1986). Mounted specimens were used for morphological observations and morphometric analyses, and kept as type specimens. All micrographs were obtained using a digital camera system (MC170 HD; Leica), and morphological drawings were made using a drawing tube connected to the microscope. To prepare the figures, the micrographs and drawings were edited using Photoshop 2019 (Adobe).
Molecular profiles and phylogeny
The nematodes transferred to NDB were digested at 55°C for 30 min, and
Natsumi Kanzaki,
Keiko Hamaguchi
Journal of Nematology, Volume 53 , 1–13
research-article | 25-May-2020
poinari (Aliramaji et al., 2014) revealed that a slightly projecting structure is present slightly anterior to the cloacal opening. This could be the single precloacal P1 papilla, or the paired papillae, similar to those in E. pini. The last pair is situated at 14.5 µm distance to the tail tip. The arrangement and number of papilla for this species are amended herein.
Molecular characterization and phylogenetic relationships
Partial SSU rDNA phylogeny
To determine the phylogenetic relationships of
Fariba Heydari,
Majid Pedram
Journal of Nematology, Volume 52 , 1–12
Research Article | 03-September-2018
Ilia Mariana Escobar-Avila,
Edgar Óliver López-Villegas,
Sergei A. Subbotin,
Alejandro Tovar-Soto
Journal of Nematology, Volume 50 , ISSUE 2, 229–242
research-article | 30-November-2019
morphometric data ranges after the type and the second Iranian population by Pedram et al. (2012)) by longer females (747 (648-920) vs 390-530 µm), longer odontostyle (7 (6-8) vs 4 µm), and total stylet (27 (24-29) vs 16-20 µm).
Molecular profiles and phylogenetic status
A 1,562 nucleotide long sequence from Oostenbrinkia pedrami n. sp. (MT860062) was used to infer the SSU phylogeny together with 28 sequences of Tylencholaimoidea and other assorted Dorylaimida (Pearse, 1942). Sequences from two species
Farahnaz Jahanshahi Afshar
Journal of Nematology, Volume 52 , 1–7
research-article | 24-November-2020
Wenhao Li,
Huixia Li,
Chunhui Ni,
Deliang Peng,
Yonggang Liu,
Ning Luo,
Xuefen Xu
Journal of Nematology, Volume 52 , 1–16
research-article | 11-March-2021
; and (VII) S. brachyurus with 0.63 posterior probability.
Figure 6:
The Bayesian tree inferred from known and newly sequenced S. brachyurus from South Africa based on COI of mtDNA region.
Discussion
Our phylogenetic analysis using 28 S rDNA and COI of mtDNA (Figures 4 and 5) placed the South African S. brachyurus populations, together with the other S. brachyurus. Additionally, based on 28 S rDNA phylogeny, S. brachyurus and S. clavicaudatum place together in a clade with support of a 1.00
Ebrahim Shokoohi
journal of nematology, Volume 53 , 1–13
Article | 21-July-2017
ILHEM GUESMI-MZOUGHI,
ANTONIO ARCHIDONA-YUSTE,
CAROLINA CANTALAPIEDRA-NAVARRETE,
HAJER REGAIEG,
NAJET HORRIGUE-RAOUANI,
JUAN E. PALOMARES-RIUS,
PABLO CASTILLO
Journal of Nematology, Volume 48 , ISSUE 3, 136–138
Article | 21-July-2017
MEHRAB ESMAEILI,
RAMIN HEYDARI,
WEIMIN YE
Journal of Nematology, Volume 48 , ISSUE 2, 109–115
Article | 24-July-2017
ALI YAGHOUBI,
EBRAHIM POURJAM,
SERGIO A LVAREZ-ORTEGA,
GRACIA LIE´BANAS,
MOHAMMAD REZA ATIGHI,
MAJID PEDRAM
Journal of Nematology, Volume 48 , ISSUE 3, 214–221
Research Article | 03-December-2018
L. K. Carta,
W. K. Thomas,
V. B. Meyer-Rochow
Journal of Nematology, Volume 50 , ISSUE 4, 479–486
research-article | 30-November-2018
Paurodontus by having the stylet with asymmetrical basal knobs (vs asymmetrical), and by the presence of a rudimentary PUS (vs prominent). It can also be distinguished from Paurodontoides by having a rudimentary PUS (vs prominent) and having a leptoderan (vs peloderan) bursa.
Molecular phylogeny
The amplification of the partial 28S D2/D3 rDNA gene sequence from Paurodontella composticola n. sp. specimens yielded a single fragment of approximately 800 bp based on gel size.
To determine the phylogenetic
Mehrab Esmaeili,
Ramin Heydari,
Ahmad Kheiri,
Weimin Ye
Journal of Nematology, Volume 51 , 1–12
research-article | 24-April-2020
phylogenetic studies have focused specifically on Cephalenchus, investigating several aspects of its evolution including inter- and intraspecies and intragenomic variations, correlation of morphology and phylogeny and biogeography (Pereira and Baldwin, 2016; Pereira et al., 2017).
During our recent surveys in South Africa, a population of Cephalenchus with a remarkably short stylet, long pharyngeal overlap, and four lines in the lateral field was recovered from the rhizosphere of grasses near a maize field
Milad Rashidifard,
Gerhard Du Preez,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–10
research-article | 30-November-2019
other Aphelenchus species is usually short, collapsed, or shrink, without sperm.
Molecular characterization and phylogeny
The sequences of nearly full-length 18S (1707 bp, GenBank accession numbers MT396110- MT396111), 28S D2-D3 region (772 bp, MT396108-MT396109) and ITS region of rDNA (661 bp, MT396103-MT396104) of A. yinyuensis n. sp. were obtained. Phylogenetic relationships among the isolates were determined separately for each dataset using Bayesian inference (BI), with Caenorhabditis
Gu Jianfeng,
Munawar Maria,
Yiwu Fang,
Liu Lele,
Xianfeng Chen,
Bo Cai
Journal of Nematology, Volume 52 , 1–12
research-article | 30-November-2020
as belonging to D. dubium. Their morphological and molecular study should elucidate the evolutionary relationships of this species, and provide new information to understand better the phylogeny of these dorylaimid taxa.
Materials and methods
Extraction and processing of nematodes
Several soil samples were collected from depths ranging from 10 to 40 cm, in the active plant root zone of East-Azarbaijan province, northwest Iran, during the period 2016 to 2017. Nematodes were extracted from soil
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Arezoo Naghavi,
Reyes Peña-Santiago
Journal of Nematology, Volume 53 , 1–12
research-article | 30-November-2020
Sergei A. Subbotin
Journal of Nematology, Volume 53 , 1–7
Article | 21-July-2017
MEHRAB ESMAEILI,
RAMIN HEYDARI,
MOZHGAN ZIAIE,
JIANFENG GU
Journal of Nematology, Volume 48 , ISSUE 1, 34–42
Article | 21-July-2017
YOUSEF PANAHANDEH,
EBRAHIM POURJAM,
MAJID PEDRAM
Journal of Nematology, Volume 48 , ISSUE 4, 268–279
research-article | 30-November-2018
Ebrahim Shokoohi,
Joaquín Abolafia,
Phatu William Mashela,
Nafiseh Divsalar
Journal of Nematology, Volume 51 , 1–26
research-article | 30-November-2018
Hossein Mirbabaei,
Ali Eskandari,
Reza Ghaderi,
Akbar Karegar
Journal of Nematology, Volume 51 , 1–14
research-article | 30-November-2020
, 1999) using the 50% majority rule. To visualize the results of each run in order to check the effective sample size of each parameter, Tracer v1.5 (Rambaut and Drummond, 2009) was used. The output file of MrBayes was visualized using Dendroscope v3.2.8 (Huson and Scornavacca, 2012) and was drawn in CorelDRAW version 17.
Table 1.
The used sequences in 28S phylogeny of Longidorella (Saevadorella) caspica n. sp., their accession number, locality, and related plants.
Species name
Accession
Fariba Heydari,
Mohammad Reza Atighi,
Ebrahim Pourjam,
Majid Pedram
Journal of Nematology, Volume 53 , 1–11
research-article | 30-November-2020
(29–30.6), b = 8.3 (7.3–9.5) vs (6.5–7.1), and longer tail length of 30 (28–31) vs ca 24.5 μm.
Bionomics
Specimens of B. hyrcanus n. sp. were successfully multiplied on a B. cinerea culture.
Molecular phylogeny
Partial sequences of the 18 S region and D2-D3 28 S expansion segments of the rDNA gene were generated with accession numbers MN888474 (730 bp) and MN888475 (718 bp), respectively. The datasets for phylogenetic trees were composed of 1,529 and 975 total characters in 18 S and D2-D3 28 S
Behrouz Golhasan,
Esmaeil Miraeiz,
Zahra Tanha maafi,
Ramin Heydari
Journal of Nematology, Volume 53 , 1–11
research-article | 05-August-2021
Bashiy Akol,
Qiuling Huang,
Borong Lin,
Honghong Wang,
Jinling Liao,
Kan Zhuo
Journal of Nematology, Volume 53 , 1–12
Article | 21-July-2017
BEHROUZ GOLHASAN,
RAMIN HEYDARI,
MEHRAB ESMAEILI,
ESMAEIL MIRAEIZ
Journal of Nematology, Volume 49 , ISSUE 1, 67–76
Research Article | 03-December-2018
Munawar Maria,
Ruihang Cai,
Weimin Ye,
Thomas O. Powers,
Jingwu Zheng
Journal of Nematology, Volume 50 , ISSUE 4, 611–622
research-article | 17-March-2020
Jianfeng Gu,
Munawar Maria,
Yiwu Fang,
Lele Liu,
Yong Bian,
Xianfeng Chen
Journal of Nematology, Volume 52 , 1–1
research-article | 30-November-2019
using CLUSTAL W (Thompson et al., 1994). The length of each alignment was 946 and 1186 bp for ITS rDNA and 28S rDNA, respectively. Bayesian inference was used to reconstruct the phylogeny, with Bayesian trees generated using the Bayesian inference method as implemented in the program MrBayes 3.1.2 (Ronquist and Huelsenbeck, 2003). The GTR + I + G model was selected using jModeltest 2.1.10 (Guindon and Gascuel, 2003; Darriba et al., 2012). Analysis using the GTR + I + G model was initiated with a
Ebrahim Shokoohi,
Phatu W. Mashela
Journal of Nematology, Volume 52 , 1–5
research-article | 06-November-2020
those in the basal clades. In the present study, we reported that a M. mali population was imported from Japan in Ningbo Port, China, with high intragenomic rRNA polymorphisms. We describe this population by detailed morphology and molecular phylogeny, and compare it with other populations in GenBank by mitochondrial COI haplotype network analysis.
Materials and methods
Isolation and morphological observation of nematodes
Roots and a little soil and rhizosphere medium associated with maple (Acer
Jianfeng Gu,
Yiwu Fang,
Lele Liu
Journal of Nematology, Volume 52 , 1–11
research-article | 30-November-2020
can be distinguished by the shape of lip region (hemispherical vs rounded), shorter body (581 (503-644) µm vs 744.0-911.0 µm), and shorter stylet (12.6 (11.5-13.5) µm vs 15.5-20.4 µm).
Habitat and locality
This population was collected in the rhizosphere of sea randa (Guettarda speciosa L.) from Ganquan Island, Sansha City, Hainan Province, China on September 23, 2019 (6.509068N, 111.596901E).
Molecular characterisation and phylogeny
The sequences of nearly full-length 18S (1649 bp, NW628178
Jianfeng Gu,
Maria Munawar,
Pablo Castillo,
Bo Cai
Journal of Nematology, Volume 53 , 1–11
research-article | 30-November-2020
(LC583316 and DQ328683), were selected for the LSU phylogeny. This dataset comprised 796 total characters. The phylogenetic tree inferred using this dataset is presented in Figure 3. The newly generated sequences of the Iranian population of B. hispaniensis have formed a maximally supported clade with other sequences of the species in this tree.
Figure 3:
Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Iranian population of Bitylenchus
Abbas Abdolkhani,
Sedighe Azimi
Journal of Nematology, Volume 53 , 1–10
research-article | 30-November-2020
, P. smoliki n. sp. displays a pattern similar to type I of Subbotin et al. (2008), with a more set-off labial disc, while the face of P. dakotaensis conform to type H, similar to that of P. flakkensis, with the labial disc not demarcated.
Phylogeny: The maximum likelihood COI tree of Pratylenchus species includes 47 specimens from Kansas and Nebraska that belong to a clade representing Pratylenchus smoliki n. sp (Figs. 6 and 7). The clade is supported by a bootstrap support value of 81, with
Thomas Powers,
Timothy Todd,
Tim Harris,
Rebecca Higgins,
Ann MacGuidwin,
Peter Mullin,
Mehmet Ozbayrak,
Kirsten Powers,
Kanan Sakai
Journal of Nematology, Volume 53 , 1–23
Article | 21-July-2017
ZAFAR A. HANDOO,
LYNN K. CARTA,
ANDREA M. SKANTAR,
SERGEI A. SUBBOTIN,
STEPHEN W. FRAEDRICH
Journal of Nematology, Volume 48 , ISSUE 1, 20–27
Research Article | 31-May-2018
Weimin Ye,
Shane Foye,
Ann E. MacGuidwin,
Shawn Steffan
Journal of Nematology, Volume 50 , ISSUE 1, 9–26
Research Article | 17-October-2018
Farzad Aliramaji,
Ebrahim Pourjam,
Sergio Álvarez-Ortega,
Farahnaz Jahanshahi Afshar,
Majid Pedram
Journal of Nematology, Volume 50 , ISSUE 3, 437–452
research-article | 30-November-2019
O. Gorgadze,
A. Troccoli,
E. Fanelli,
E. Tarasco,
F. De Luca
Journal of Nematology, Volume 52 , 1–12
research-article | 30-November-2020
; longitude – 111° 56′ 37.5″ W); Taber Rural Municipality, Alberta, Canada.
Molecular characterization and phylogeny
The sequences of partial 18S (GenBank accession numbers MW029450, MW029451 for G. brevidens; MW023248, MW023249 for Q. capitatus), 28S (MW029449 for G. brevidens, MW023387 for Q. capitatus), and the ITS region of the rRNA (MW029446, MW029447, MW029448 for G. brevidens; MW027537, MW027538 for Q. capitatus) of both species were obtained.
Phylogenetic relationships among the isolates were
Maria Munawar,
Dmytro P. Yevtushenko,
Pablo Castillo
Journal of Nematology, Volume 53 , 1–17
research-article | 30-November-2020
Tripylina clade (Figs 4 and 5). BLAST analysis revealed the sequence identity between T. gorganensis populations from Iran and Slovakia of more than 99% (4 bp difference) in case of 18 S rDNA and of 97–98% (16–20 SNPs) in case of 28 rDNA.
Figure 4:
18 S rDNA-based Bayesian phylogeny of the Trischistomatidae and some other representatives of Enoplida. The new population of Tripylina gorganensis is indicated in bold. Numbers near nodes indicate posterior probabilities. ♀♂ = all species from the marked
Marek Renčo,
Katarzyna Rybarczyk-Mydłowska,
Łukasz Flis,
Magdalena Kubicz,
Grażyna Winiszewska
Journal of Nematology, Volume 53 , 1–10
research-article | 30-November-2018
provided its diagnosis.
Sudhaus and Hooper (1994) provided new ideas about the taxonomy and the phylogeny of several rhabditid species: (i) accepted Oscheius as a subgenus of Rhabditis, (ii) regarded it as a monophyletic taxon based on three synapomorphies (long female rectum, terminal duct of the excretory system forwards coiled and with heavily sclerotized wall, and several features of spicule shape), (iii) considered Dolichorhabditis (Andrássy, 1983) as a junior synonym of Oscheius, and (iv
Joaquín Abolafia,
Reyes Peña-Santiago
journal of nematology, Volume 51 , 1–21
Article | 21-July-2017
HARUN CIMEN,
VLADIMI´R PU°zA,
JIRI NERMUT,
JUSTIN HATTING,
TSHIMA RAMAKUWELA,
SELCUK HAZIR
Journal of Nematology, Volume 48 , ISSUE 3, 148–158
Article | 21-July-2017
MANUEL MUNDO-OCAMPO,
J. G. BALDWIN,
T. J. PEREIRA,
J. R. CAMACHO-BAEZ,
A. D. ARMENTA-BOJORQUEZ,
M. CAMACHO-HARO,
J. O. BECKER
Journal of Nematology, Volume 49 , ISSUE 1, 103–113
research-article | 30-November-2018
. lapidosum seems to be related with X. lupini because of both species clustered together in all the analyses carried out in this study. The present study on the phylogeny based on D2 to D3 segments supported a very weak correlation in the phylogenetic relationships among the different morphospecies groups within Xiphinema, a finding already reported by several authors namely, Gutiérrez-Gutiérrez et al., 2013; De Luca et al., 2014; Tzortzakakis et al., 2014, 2015; Archidona-Yuste et al., 2016a, 2016b
Ruihang Cai,
Antonio Archidona-Yuste,
Carolina Cantalapiedra-Navarrete,
Juan E. Palomares-Rius,
Jingwu Zheng,
Pablo Castillo
Journal of Nematology, Volume 51 , 1–21
research-article | 30-November-2018
-separated clades and grouped with species having shorter stylets and advuval flap, the species having longer stylets arranged at the basal position of the trees. To this point we suppose only fewer paratylenchid sequences were available for the phylogenetic studies, the inclusion of more sequences from other known species will be helpful to provide a better resolution of paratylenchid phylogeny.
Additional remarks
Ghaderi et al. (2014) presented a key for the species identification of pin nematodes
Munawar Maria,
Wentao Miao,
Weimin Ye,
Jingwu Zheng
journal of nematology, Volume 51 , 1–13
research-article | 30-November-2018
Alemayehu W. Habteweld,
Faruk Akyazi,
Soumi Joseph,
William T. Crow,
Eyualem Abebe,
Tesfamariam Mekete
Journal of Nematology, Volume 51 , 1–15
research-article | 30-November-2019
corms and roots of banana where they were likely to cause damage to the crop (Sikora et al., 2018). Phylogenetic analysis of Hoplolaimus spp. using D2–D3 expansion of 28 S and internal transcribed spacer (ITS1) ribosomal DNA sequences resolved the phylogeny of the genus and were useful in molecular identification of Hoplolaimus spp. (Bae et al., 2008). In addition, the PCR-RFLP method was applied by different researchers to evaluate the genetic diversity of Hoplolaimus spp. (Robbins et al., 2009
Mariette Marais,
Esther van den Berg,
Hendrika Fourie,
Milad Rashidifard
Journal of Nematology, Volume 52 , 1–12
research-article | 30-November-2019
cylindroid anteriorly, mostly narrowing to a bluntly triangular or wedge-shaped posterior part).
From H. dulli Van den Berg & Tiedt, 2001, a species with close phylogenetic affinities in ITS phylogeny, by shorter stylet (63.3–71.0 vs 73–79 μm), lower Rst (18–21 vs 21–25), lateral field lacking line(s) (vs having one or two lines), lip region continuous with one annulus (vs set off with two annuli), excretory pore located posterior to pharynx base (vs anterior or posterior) and vulva with not or slightly
Sedighe Azimi,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–19
research-article | 30-November-2019
are related in the clade with N. major (PP = 100). N. hades differs from N. cancellata by 16 bp (2.1%) and from N. major by 11 bp (1.6%), and N. major differs from N. cancellata by 20 bp (1.9%).
Discussion
In our 28 S rDNA phylogeny, Neothada is highly supported as a valid genus (Figure 6), similar to previously published works (Yaghubi et al., 2015; Hosseinvand et al., 2020a), and as a member of the subfamily Boleodorinae as suggested by Geraert (2008). In this tree, Neothada formed a sister
Manouchehr Hosseinvand,
Ali Eskandari,
Reza Ghaderi
Journal of Nematology, Volume 52 , 1–10
research-article | 30-November-2020
delimitation of the species, assist in explaining the tentative synonymies (Pedram et al., 2018) and will further clarify the phylogeny of the genus.
The genus is known in South Africa by only one representative, L. patulus (Swart, 1997), which has been described based on the traditional criteria. During the present study, a population of the genus, representing an unknown species, was recovered from wood samples of a dead maritime pine tree in Potchefstroom, North-West province, South Africa. Comparisons
Farahnaz Jahanshahi Afshar,
Milad Rashidifard,
Joaquín Abolafia,
Miloslav Zouhar,
Hendrika Fourie,
Majid Pedram
Journal Of Nematology, Volume 53 , 1–14
Research Article | 03-September-2018
Farshad Gharibzadeh,
Ebrahim Pourjam,
Majid Pedram
Journal of Nematology, Volume 50 , ISSUE 2, 207–218
Research Article | 17-October-2018
Yousef Panahandeh,
Joaquín Abolafia,
Ebrahim Pourjam,
Robin M. Giblin-Davis,
Farahnaz Jahanshahi Afshar,
Majid Pedram
Journal of Nematology, Volume 50 , ISSUE 3, 343–354
research-article | 18-March-2020
-morphological characterization presenting challenges in the taxonomy of criconematids. The phylogeny of the majority of criconematids taxa is not well resolved, and to this point, we only suggest that molecular identification can be an efficient way of identifying species; however, linking the correct molecular information to the detected species is an important aspect. The generic status of new species is assigned primarily on the basis of morphological characters of females and juveniles. This is the
Munawar Maria,
Wentao Miao,
Ruihang Cai,
Pablo Castillo,
Jingwu Zheng
Journal of Nematology, Volume 52 , 1–14
research-article | 16-April-2020
, additional studies are required to shed light on the evolution, phylogeny, and ecological aspects of these nematodes.
Munawar Maria,
Wentao Miao,
Pablo Castillo,
Jingwu Zheng
Journal of Nematology, Volume 52 , 1–13
research-article | 01-October-2021
Joaquín Abolafia,
Matteo Vecchi
Journal of Nematology, Volume 53 , 1–20
Article | 21-July-2017
MAGDALENA OLSON,
TIMOTHY HARRIS,
REBECCA HIGGINS,
PETER MULLIN,
KIRSTEN POWERS,
SEAN OLSON,
THOMAS O. POWERS
Journal of Nematology, Volume 49 , ISSUE 1, 42–66
Article | 04-December-2017
MEHRAB ESMAEILI,
RAMIN HEYDARI,
WEIMIN YE
Journal of Nematology, Volume 49 , ISSUE 3, 268–275
research-article | 30-November-2019
Journal of Nematology, Volume 52 , 1–2
research-article | 30-November-2019
fields (6 vs 10-12) and anteriorly located vulva (V = 89 (87.2-90.3) vs 92 (92-93)).
Molecular profiles and phylogenetic status
Nearly full length SSU rDNA phylogeny
To determine the phylogenetic relationships of D. brevis n. sp. with other species, a newly obtained 1674 nt nearly full length SSU rDNA sequence with accession number MT009494 was used. The BLAST search using this fragment revealed that it is unique, and its identity with other previously submitted sequences to the database is less
Fariba Heydari,
Joaquín Abolafia,
Majid Pedram
Journal of Nematology, Volume 52 , 1–13
research-article | 30-November-2020
Magdalena Lis,
Ewa Sajnaga,
Marcin Skowronek,
Adrian Wiater,
Kamila Rachwał,
Waldemar Kazimierczak
Journal of Nematology, Volume 53 , 1–24
Research Article | 26-September-2018
LIRONG ZHAO,
WEIMIN YE,
MUNAWAR MARIA,
MAJID PEDRAM,
JIANFENG GU
Journal of Nematology, Volume 49 , ISSUE 4, 404–417
research-article | 24-April-2019
morphology and morphometrics along with molecular characteristics and phylogeny of the D2-D3 expansion segment of 28S rDNA, ITS rDNA, and COI mtDNA sequences.
Materials and methods
Sampling and nematode extraction
The soil and root samples were collected around the rhizosphere of banana (Musa basjoo Siebold & Zucc. ex Iinuma) (GPS coordinates N: 51°2′6.8″, E: 3°43′22.7″) and Yam (Dioscorea tokoro) (GPS coordinates: N: 51°2′6.9″, E: 3°43′22.6″) at the Botanical garden of Ghent University. The nematodes
Huu Tien Nguyen,
Quang Phap Trinh,
Marjolein Couvreur,
Phougeishangbam Rolish Singh,
Wilfrida Decraemer,
Wim Bert
Journal of Nematology, Volume 51 , 1–20
research-article | 30-November-2020
Joaquín Abolafia,
Manouchehr Hosseinvand,
Ali Eskandari
Journal of Nematology, Volume 53 , 1–16
research-article | 22-February-2021
Phougeishangbam Rolish Singh,
Gerrit Karssen,
Kelvin Gitau,
Cecilia Wanjau,
Marjolein Couvreur,
Njira Njira Pili,
Godelieve Gheysen,
Wim Bert
Journal of Nematology, Volume 53 , 1–14
research-article | 30-November-2019
Phougeishangbam Rolish Singh,
Beatrice E. Kashando,
Marjolein Couvreur,
Gerrit Karssen,
Wim Bert
Journal of Nematology, Volume 52 , 1–17
research-article | 11-January-2021
Sergei A. Subbotin,
Guiping Yan,
Mihail Kantor,
Zafar Handoo
Journal of Nematology, Volume 52 , 1–7
research-article | 30-November-2019
Yao A. Kolombia,
Oluwadamilola Ogundero,
Emmanuel Olajide,
Nicole Viaene,
P. Lava Kumar,
Danny L. Coyne,
Wim Bert
Journal of Nematology, Volume 52 , 1–25
research-article | 30-November-2018
the most consistent tree representing the relationship of the P. curvicauda samples with other Pratylenchus species. These trees were constructed using the bootstrap method as a test of Phylogeny with 1,000 replicates, where necessary they are presented with the bootstrap values. Representative sequences of Meloidogyne spp. and Radopholus similis, derived from similar regions of the rDNA, were used as outgroups for the phylogenetic analyses.
Differences between and within sequences of either the
Farhana Begum,
John Fosu-Nyarko,
Shashi Sharma,
Bill Macleod,
Sarah Collins,
Michael G. K. Jones
Journal of Nematology, Volume 51 , 1–15
research-article | 30-November-2020
described species P. nanjingensis which has a 64–68 µm long stylet grouped with P. bilineatus and P. aculentus. In another study, Wang et al. (2016b) described P. guangzhouensis, a species with the stylet averaging 47 µm long and based on their ITS rRNA phylogeny, the authors showed that this species was clustered with those four species having a long stylet. Recently, Munawar et al. (2021), Singh et al. (2021), Clavero-Camacho et al. (2021) published comprehensive phylogenies of the genus Paratylenchus
Mihail R. Kantor,
Zafar A. Handoo,
Sergei A. Subbotin,
Gary R. Bauchan,
Joseph D. Mowery
Journal of Nematology, Volume 53 , 1–10