Article | 21-July-2017
N. CAROL CASAVANT,
JOSEPH C. KUHL,
FANGMING XIAO,
ALLAN B. CAPLAN,
LOUISE-MARIE DANDURAND
Journal of Nematology, Volume 49 , ISSUE 1, 12–20
Original Paper | 04-September-2018
mRNA, inhibition to DNA polymerase, and degradation of mRNA for determining the RNA quality using standard RNA. It would be possible to know yield of mRNA and inhibition of the enzyme reaction by adding standard RNA before RNA extraction and looking at standard RNA loss. Degradation was evaluated by comparing the differences in the 3’ and 5’ regions of the RNA. In our study, it was demonstrated that in the crude extract of Saccharomyces cerevisiae, degradation was comparatively higher at the 3’ end
TAKEMA HASEGAWA,
JUNKO TAKAHASHI,
HITOSHI IWAHASHI
Polish Journal of Microbiology, Volume 67 , ISSUE 3, 347–353
research-article | 07-July-2020
animal performed the movements, using Pluz-MZ v1.1 Software. During all the evaluation period, groups were randomly tested and the investigator was unaware to the experimental group’s composition.
RNA extraction
Five animals from each group were submitted to RNA extraction and gene expression analyses. Animals were euthanized through deep isoflurane inhalation. After brain removal, hippocampus bilateral dissection was performed using a stereomicroscope. Then, the tissue was placed into a
Gabrielle Pfutzenreuter,
Kenny Nieradka,
Márcia Regina Pincerati,
Ilton Santos da Silva
Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 2, 160–169