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research-article | 30-November-2020

Recombinase Polymerase Amplification assays for detection of the major tropical root-knot nematodes

and plant roots is important for the application of effective control measures and prevention of spread of these agricultural pests. Currently, the reliable diagnosis of RKN is based on molecular methods using sequencing of nuclear ribosomal RNA and mitochondrial genes, PCR with specific primers and other techniques (Subbotin et al., 2021). Recombinase Polymerase Amplification (RPA) is a relatively new isothermal methodology for amplifying DNA and uses a highly efficient displacement polymerase

Sergei A. Subbotin, Julie Burbridge

Journal of Nematology, Volume 53 , 1–11

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