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research-article | 17-March-2020

Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

USA where related species may exist. Molecular and morphological taxonomic identifications were conducted in our lab with the nematodes isolated from the lesions of the BLD leaves collected in Fall, 2017 from Perry, Lake County, Ohio, USA by an Ohio Department of Agriculture nursery inspector from ailing American beech trees Fagus grandifolia (Fall specimens). Their ribosomal DNA (rDNA) loci were amplified by PCR with the one primer set and an enhanced DNA polymerase system, and the resulting 3.5

L.K. Carta, S. Li

Journal of Nematology, Volume 52 , 1–15

research-article | 03-June-2019

PCR amplification of a long rDNA segment with one primer pair in agriculturally important nematodes

Eukaryotic nuclear ribosomal DNA (rDNA) is arranged in tandem repeat arrays in the genome. Each repeat unit consists of one copy of small subunit (SSU) 18S, internal transcribed spacers (ITS1 and ITS2), 5.8S, and large subunit (LSU) 28S rDNA, and is separated by an external transcribed spacer (EST) and an intergenic spacer (IGS) (Hillis and Dixon, 1991). The copy number of the repeats within most eukaryotic genomes is high, which provide large quantities of template DNA for PCR. In

L. K. Carta, S. Li

Journal of Nematology, Volume 51 , 1–8

Article | 21-July-2017

Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves

northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the


Journal of Nematology, Volume 48 , ISSUE 1, 28–33

research-article | 24-April-2020

Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Milad Rashidifard, Gerhard Du Preez, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–10

Original Paper | 10-December-2018

Epilithic Biofilms in Lake Baikal: Screening and Diversity of PKS and NRPS Genes in the Genomes of Heterotrophic Bacteria

Abstract A collection of heterotrophic bacteria consisting of 167 strains was obtained from microbial communities of biofilms formed on solid substrates in the littoral zone of Lake Baikal. Based on the analysis of 16S rRNA gene fragments, the isolates were classified to four phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. To assess their biotechnological potential, bacteria were screened for the presence of PKS (polyketide synthase) and NRPS (non-ribosomal peptide


Polish Journal of Microbiology, Volume 67 , ISSUE 4, 501–516

Research Article | 03-September-2018

Molecular Identification of Entomopathogenic Nematode Isolates from the Philippines and their Biological Control Potential Against Lepidopteran Pests of Corn

In search for local entomopathogenic nematode (EPN) species as a biological control agent of lepidopterous insect pests of corn, a survey for EPN in the major islands in the Philippines was conducted. Seven EPN populations from 279 soil samples were isolated using Ostrinia furnacalis, the key target insect pest of corn in the country, as bait. Analysis of the ITS1-5.8S-ITS2 ribosomal DNA sequence revealed the presence of Steinernema abbasi, Steinernema minutum, Steinernema tami, and

Barbara L. Caoili, Romnick A. Latina, Regina Faye C. Sandoval, Joey I. Orajay

Journal of Nematology, Volume 50 , ISSUE 2, 99–110

research-article | 30-November-2020

Morphological and molecular characterization of Iotonchus lotilabiatus n. sp. (Nematoda: Iotonchidae) from Lao Cai Province, Vietnam

Tam T. T. Vu, Thi Mai Linh Le, Thi Duyen Nguyen

Journal of Nematology, Volume 53 , 1–22

research-article | 30-November-2020

Description of Prionchulus jonkershoekensis n. sp. (Nematoda: Mononchida), a new predatory species from South Africa

Candice Jansen van Rensburg, Hendrika Fourie, Samad Ashrafi, Milad Rashidifard

Journal of Nematology, Volume 53 , 1–10

research-article | 30-November-2020

Descriptions of two new and one known species of Parkellus Jairajpuri, Tahseen and Choi, 2001 (Nematoda: Mononchidae) and their phylogenetic position among Mononchida

Parkellus rDNA sequence fragments, as well as ribosomal DNA fragments of Coomansus parvus used in this study as a close reference species, were deposited in GenBank under the following accession numbers: MT799665–MT799669 (18S rDNA) and MT799670–MT799673 (28S rDNA). Sequence analysis and phylogenetic studies The newly obtained rDNA sequences were analyzed using the BioEdit program (Hall, 1999: v.7.2.5). The final 18S and 28S rDNA datasets for phylogenetic study included sequences from the three

Tam T.T. VU, Katarzyna Rybarczyk-Mydłowska, Andrij Susulovsky, Magdalena Kubicz, Łukasz Flis, Thi Mai Linh Le, Grażyna Winiszewska

Journal of Nematology, Volume 53 , 1–22

research-article | 13-April-2020

First molecular characterization of an Iranian population of Schistonchus caprifici (Gasparrini, 1864) Cobb, 1927 (Rhabditida; Aphelenchoidea)

in Estahban (Fars province) during the present study, the morphological and morphometric characters of this population were found to be in accordance with the data presented by Hunt (1993), Vovlas and Larizza (1996) and Kolaei et al. (2016). Therefore, the present study aimed to characterize this recently recovered population for its molecular identity by sequencing the D2-D3 expansion fragments of the large subunit of the ribosomal DNA gene, as the first molecular analysis of the Iranian

Hadi Karimipour Fard, Hamid Zare

Journal of Nematology, Volume 52 , 1–3

Research Article | 03-December-2018

Two nematodes (Nematoda: Diplogastridae, Rhabditidae) from the invasive millipede Chamberlinius hualienensis Wang, 1956 (Diplopoda, Paradoxosomatidae) on Hachijojima Island in Japan

L. K. Carta, W. K. Thomas, V. B. Meyer-Rochow

Journal of Nematology, Volume 50 , ISSUE 4, 479–486

research-article | 30-November-2019

Four Pristionchus species associated with two mass-occurring Parafontaria laminata populations

Natsumi Kanzaki, Minami Ozawa, Yuko Ota, Yousuke Degawa

Journal of Nematology, Volume 52 , 1–10

research-article | 30-November-2020

First report of Cactodera milleri Graney and Bird, 1990 from Colorado and Minnesota

county, Colorado, USA. A, B: J2 anterior ends; C, D: J2 tails; E: J2 lateral field; F: Cyst posterior (ventral view); G: Cyst cone mount. Representative J2 from two CO isolates and three MN isolates were used for molecular confirmation of the species, using two ribosomal genes (internal transcribed spacer, ITS 1 and 2 and large ribosomal subunit, 28S), one nuclear gene (partial heat shock protein 90, Hsp90), and one mitochondrial gene (partial cytochrome oxidase I, COI). Markers were amplified

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Saad L. Hafez, Maria N. Hult, Kathryn Kromroy, Kimberly Sigurdson, Michelle Grabowski

Journal of Nematology, Volume 53 , 1–7

research-article | 30-November-2018

The morphological characteristics and phylogenetic analysis of Pratylenchus vulnus Taiwan strawberry isolate

1000X magnification with compound microscope and SEM. Molecular analysis of the ribosomal RNA and mitochondrial gene sequences of the extracted nematodes confirmed their species as P. vulnus (Table 1). The rDNA LSU region (D2A/D3B: ACAAGTACCGTGAGGGAAAGTTG/TCGGAAGGAACCAGCTACTA) (Nunn, 1992), rDNA ITS region (TW81/AB28: GTTTCCGTAGGTGAACCTGC/ATATGCTTAAGTTCAGCGGGT) (Amiri et al., 2002; Subbotin et al., 2001), rDNA SSU region (SSU18A/SSU26R: AAAGATTAAGCCATGCATG/CATTCTTGGCAAATGCTTTCG) (Eyualem and

Yu-po Lin, Wan-chun Lee, Pei-che Chung, Jiue-in Yang

Journal of Nematology, Volume 51 , 1–5

Original Research | 11-December-2017

Description of Pseudacrobeles (Pseudacrobeles) curvatus sp. n. (Cephalobidae: Rhabditida) in South Korea

and diagnostic features of Pseudacrobeles species and molecular sequence data from the D2-D3 regions of the 28S ribosomal DNA (rDNA) and ITS1-5.8S-ITS2 region of rDNA from the new species, which can be used as molecular barcode sequences.  

Jiyeon Kim, Taeho Kim, Joong-Ki Park

Journal of Nematology, Volume 49 , ISSUE 2, 162–167

research-article | 30-November-2018

First record of Aphelenchoides stammeri (Nematoda: Aphelenchoididae) from Turkey

of Aphelenchoides stammeri are very similar to the original description of the species by Körner (1954). Confirmation of the morphological description was made by H. Braasch (pers. Comm. with H. Braasch). Molecular characterization The length of sequenced 18S rRNA was 641 bp and matched with A. stammeri gene for 18S ribosomal RNA, partial sequence (AB368535.1) (99% identify, e-value 0.0 and 0% gaps), and the length of sequenced 28S rRNA was 618 and matched with A. stammeri partial 28S rRNA gene

Mehmet Dayi, Ece B. Kasapoğlu Uludamar, Süleyman Akbulut, İ. Halil Elekcioğlu

journal of nematology, Volume 51 , 1–6

research-article | 30-November-2019

Morphological and molecular characterization of Heterodera dunensis n. sp. (Nematoda: Heteroderidae) from Gran Canaria, Canary Islands

field differentiation, tail length, and hyaline tail length in J2 (Subbotin et al., 2010). Since the last two decades, employing molecular data such as ITS and 28S of ribosomal DNA and COI gene of mitochondrial DNA to characterize Heterodera species has been a common practice, including DNA barcoding, phylogeny, and even phylogeography (Ferris et al., 1999; Toumi et al., 2013a; Subbotin et al., 2017, 2018). Herein, we characterize Heterodera dunensis n. sp. discovered in a recent exploratory survey

Phougeishangbam Rolish Singh, Gerrit Karssen, Marjolein Couvreur, Wim Bert

Journal of Nematology, Volume 52 , 1–14

Research Article | 03-December-2018

Improved 18S small subunit rDNA primers for problematic nematode amplification

The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new

L. K. Carta, S. Li

Journal of Nematology, Volume 50 , ISSUE 4, 533–542

Article | 21-July-2017

Sectonema caobangense sp. n. from Vietnam (Nematoda, Dorylaimida, Aporcelaimidae)


Journal of Nematology, Volume 48 , ISSUE 2, 95–103

research-article | 30-November-2020

Morphological and molecular characterisation of Tripylina gorganensis from the Slovak Republic as a contribution to the redescription of the species

. yeatesi Zhao, 2009 New Zealand T. ymyensis Tahseen & Nusrat, 2010 China T. zhejiangensis Pham et al., 2013 China Starting with the work by Zhao (2009), half of the described species have been characterized molecularly by partial sequences of either one or two of the ribosomal DNA genes (18 S and/or 28 S rDNA). The rDNA-based phylogenetic analyses revealed the well-supported monophyletic status of Tripylina (Asghari et al., 2012; Cid del Prado-Vera et al., 2012, 2016; Xu et al., 2013

Marek Renčo, Katarzyna Rybarczyk-Mydłowska, Łukasz Flis, Magdalena Kubicz, Grażyna Winiszewska

Journal of Nematology, Volume 53 , 1–10

research-article | 30-November-2018

First report of Longidorus mindanaoensis Coomans, De Ley, Jimenez and De Ley, 2012 (Nematoda: Longidoridae) From a Mangrove Forest in Vietnam

Thi Duyen Nguyen, Huu Tien Nguyen, Thi Mai Linh Le, Neriza Nobleza, Quang Phap Trinh

journal of nematology, Volume 51 , 1–5

research-article | 30-November-2019

Morphological and molecular characterization of Hoplolaimus pararobustus (Schuurmans Stekhoven and Teunissen, 1938) Sher 1963 with its first report on Zea mays roots in Namibia

corms and roots of banana where they were likely to cause damage to the crop (Sikora et al., 2018). Phylogenetic analysis of Hoplolaimus spp. using D2–D3 expansion of 28 S and internal transcribed spacer (ITS1) ribosomal DNA sequences resolved the phylogeny of the genus and were useful in molecular identification of Hoplolaimus spp. (Bae et al., 2008). In addition, the PCR-RFLP method was applied by different researchers to evaluate the genetic diversity of Hoplolaimus spp. (Robbins et al., 2009

Mariette Marais, Esther van den Berg, Hendrika Fourie, Milad Rashidifard

Journal of Nematology, Volume 52 , 1–12

research-article | 17-March-2020

Description of Laimaphelenchus sinensis n. sp. (Nematoda: Aphelenchoididae) from declining Chinese pine, Pinus tabuliformis in Beijing, China

by Majorbio, Shanghai, China) were used in the PCR analyses to amplify the near full length 18 S, full length ITS region and D2-D3 expansion segments of the 28 S ribosomal RNA genes (rDNA). The near full length 18 S region was amplified as two partially overlapping fragments; for the first fragment, 988 F (5-CTC AAA GAT TAA GCC ATG C-3) and 1912R (5-TTT ACG GTC AGA ACT AGG G-3) were used and for the second fragment 1813F (5-CTG CGT GAG AGG TGA AAT-3) and 2646 R (5-GCT ACC TTG TTA CGA CTT TT-3

Jianfeng Gu, Munawar Maria, Yiwu Fang, Lele Liu, Yong Bian, Xianfeng Chen

Journal of Nematology, Volume 52 , 1–1

research-article | 30-November-2019

An integrative approach to the study of Helicotylenchus (Nematoda: Hoplolaimidae) Colombian and Brazilian populations associated with Musa crops

% Triton x–100, 4.5% Tween–20, 0.09% Proteinase K). The tubes were incubated at −80°C (15 min), 65°C (1 h), and 95°C (15 min), centrifuged to 16,000 g (1 min) and stored at −20°C. Amplification of D2 to D3 expansion segment of the large subunit – LSU of ribosomal DNA (28S) was done using forward primer D2A (5′–ACAAGTACCGTGAGGGAAAGTTG–3′) and reverse D3B (5′–TCCTCGGAAGGAACCAGCTACTA–3′) (De Ley et al., 1999). The PCR conditions were initial denaturation during 2 min at 94°C followed by 40 cycles of 45 s

Donald Riascos-Ortiz, Ana Teresa Mosquera-Espinosa, Francia Varón De Agudelo, Claudio Marcelo Gonçalves de Oliveira, Jaime Eduardo Muñoz-Florez

Journal of Nematology, Volume 52 , 1–19

research-article | 15-August-2017

Description and Distribution of Three Criconematid Nematodes from Hangzhou, Zhejiang Province, China

Maria Munawar, Thomas O. Powers, Zhongling Tian, Timothy Harris, Rebecca Higgins, Jingwu Zheng

Journal of Nematology, Volume 50 , ISSUE 2, 183–206

Article | 21-July-2017

Occurrence of Panagrellus (Rhabditida: Panagrolaimidae) Nematodes in a Morphologically Aberrant Adult Specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)

and 18S ribosomal DNA and shown to belong to the family Panagrolaimidae (Rhabditida), within a clade of Panagrellus. While most nematodes in the insect were juveniles, a single male adult was partially characterized by light microscopy. Morphometrics showed similarities to a species described from Germany. Excluding the entomopathogenic nematodes (EPN), only five other genera of entomophilic or saprophytic rhabditid nematodes are associated with this weevil. This is the first


Journal of Nematology, Volume 48 , ISSUE 1, 1–6

Research Article | 03-December-2018

First report of Pratylenchus vulnus associated with apple in Tunisia

. Microscopic observation of females and males demonstrated the occurrence of Pratylenchusd vulnus on apple trees. The ribosomal DNA D2-D3 expansion segments of the 28S rRNA and of the Pratylenchus populations were PCR amplified and sequenced. The sequences were compared with those of Pratylenchus species in the GenBank database with high similarity (99%). This comparison reconfirmed the morphological identifications. Phylogenetic studies placed those populations with P. vulnus. This is the first report of

Noura Chihani-Hammas, Lobna Hajji-Hedfi, Hajer Regaieg, Asma Larayedh, Ahmed Badiss, Yu Qing, Horrigue-Raouani Najet

Journal of Nematology, Volume 50 , ISSUE 4, 579–586

research-article | 30-November-2019

Oostenbrinkia pedrami n. sp. (Dorylaimida: Aulolaimoididae) from Iran, with molecular phylogenetic relationships to some other Dorylaimida Pearse, 1942

) buffer (10 mM Tris-Cl, 0.5 mM EDTA, pH 9.0, Qiagen) on a clean slide, and squashed using a clean slide cover with the aid of a pipette tip. The suspension was collected by adding 20 μl of TE buffer after gently removing the slide cover and leaving the solution on the slide (Pedram, 2017). The DNA sample was stored at −20°C until used as the polymerase chain reaction (PCR) template. The near-full-length sequence of the small subunit ribosomal DNA (SSU rDNA) was amplified using the forward primer 18S4

Farahnaz Jahanshahi Afshar

Journal of Nematology, Volume 52 , 1–7

original-paper | 27-December-2020

Identification and Characterization of a Novel Recombinant Porcine Astrovirus from Pigs in Anhui, China

%, and 22.21%, respectively. The viral genome of Ahast encodes three ORFs, including ORF1a, ORF1b, and ORF2. There is a 8 nt overlap between ORF1b and ORF2. The conserve Kozak sequences of RNNAUGG were identified near the start codon of both three ORFs (GCTATGG for ORF1a, AAAATGT for ORF1b, and CTAATGG for ORF2). A translational ribosomal frameshifting signal existed in the 3' end of Ahast ORF1a Fig. 1a. The Conserved Domains analysis ( indicated


Polish Journal of Microbiology, Volume 69 , ISSUE 4, 471–478

research-article | 30-November-2020

Pristionchus trametes n. sp. (Diplogastridae) isolated from the mushroom Trametes orientalis in Kyoto, Japan

., 2021; Mayer et al., 2007), the species diversity of the genus is still far from saturated (Kanzaki et al., 2021; Rödelsperger et al., 2018), and further isolation of various species is necessary to improve the model system.This study describes a species of Pristionchus recovered from fruiting bodies (mushrooms) of the wood-decaying fungus Trametes orientalis (Yasuda) based on its typological characters and ribosomal RNA sequences, which were used as species-specific molecular barcodes. Materials

Natsumi Kanzaki, Keiko Hamaguchi

Journal of Nematology, Volume 53 , 1–13

Article | 21-July-2017

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

-pharynx (D% = 46). Hyaline layer occupies approximately half of tail length. Male spicules slightly to moderately curved, with a sharp tip and golden brown in color. The first generation of males lacking a mucron on the tail tip while the second generation males with a short filamentous mucron. Genital papillae with 11 pairs and one unpaired preanal papilla. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions (D2-D3) of the ribosomal


Journal of Nematology, Volume 48 , ISSUE 3, 148–158

research-article | 30-November-2019

Additional notes on the morphology and molecular data of the Kikuyu root-knot nematode, Meloidogyne kikuyensis (Nematoda: Meloidogynidae)

ribosomal RNA gene (COII-16S) was amplified using the forward C2F3 (5´-GGT CAA TGT TCA GAA ATT TGT GG-3´) and reverse 1108 (5´-TAC CTT TGA CCA ATC ACG CT-3´) primers (Powers and Sanders, 1993). The following primers were used for the amplification of the ITS1 region, including partial sequences of the 18S and 5.8S rRNA genes: 5´-TTG ATT ACG TCC CTG CCC TTT-3´ (Vrain et al., 1992) and 5´-ACG AGC CGA GTG ATC CAC CG-3´ (Cherry et al., 1997). The obtained sequences for M. kikuyensis were deposited in

J. D. Eisenback, P. Vieira

Journal of Nematology, Volume 52 , 1–13

research-article | 30-November-2020

Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Mihail R. Kantor, Zafar A. Handoo, Sergei A. Subbotin, Gary R. Bauchan, Joseph D. Mowery

Journal of Nematology, Volume 53 , 1–10

research-article | 18-March-2020

Description of Seinura italiensis n. sp. (Tylenchomorpha: Aphelenchoididae) found in the medium soil imported from Italy

Jianfeng Gu, Munawar Maria, Lele Liu, Majid Pedram

Journal of Nematology, Volume 52 , 1–11

research-article | 22-February-2021

Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

accepted and its widespread use has also recently been facilitated by a web-based key that draws its basis from cluster analysis (Nguyen et al., 2019). In combination with the morphological features, molecular information such as ITS, 18S, and 28S of ribosomal DNA and COI of mitochondrial DNA have been used for their identification. In the current paper, we characterize a newly discovered Rotylenchus wimbii n. sp. found associated with finger millet, Eleusine coracana (L.) Gaertn. (Planta: Poaceae) in

Phougeishangbam Rolish Singh, Gerrit Karssen, Kelvin Gitau, Cecilia Wanjau, Marjolein Couvreur, Njira Njira Pili, Godelieve Gheysen, Wim Bert

Journal of Nematology, Volume 53 , 1–14

research-article | 30-November-2020

Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia

mM Tris pH 8.0, 15 mM MgCl2, 0.5% Triton × 100, 4.5% Tween 20, and 0.09% Proteinase K). Subsequently, the tubes were incubated at ‒80°C for 15 min, 65 °C for 1 h, and 95°C for 15 min, centrifuged at 16,000 × g for 1 min, and stored at ‒20°C. The polymerase chain reaction (PCR) amplification of the expansion segment D2-D3 of the large subunit of ribosomal DNA (28S) was performed with the primers D2A (5′-ACAAGTACCGTGAGGGAAAGTTG-3′) forward and D3B (5′-TCCTCGGAAGGAACCAGCTACTA-3′) reverse, according

Oscar Velandia, Yuri Mestizo, Héctor Camilo Medina, Donald Riascos-Ortiz, Francia Varón De Agudelo, Greicy Andrea Sarria

Journal of Nematology, Volume 53 , 1–14

Article | 21-July-2017

Occurrence of Belonolaimus in Sinaloa, Northwestern Mexico: A New Report on Distribution and Host Range

to the Belonolaimus longicaudatus species complex. Molecular analyses based on the 28S gene and ITS1-5.8S-ITS2 regions of the ribosomal RNA (rRNA) identified four major clades within Belonolaimus; however, none of the species including B. longicaudatus, B. gracilis, and B. euthychilus were supported as monophyletic; yet monophyly is argued to be a basic requirement of species status. Sequence divergence among different Belonolaimus populations and species varied according to the rRNA dataset (i.e


Journal of Nematology, Volume 49 , ISSUE 1, 103–113

research-article | 17-March-2020

Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

Doreen M. Mgonja, Gladness E. Temu, Joseph C. Ndunguru, Magreth F. Mziray, Sylvester L. Lyantagaye, Nessie D. Luambano

Journal of Nematology, Volume 52 , 1–8

research-article | 30-March-2020

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

, 0.2 mM dNTPs, 1U Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA), 3 µl nematode DNA extract, and supplied enzyme reaction buffer in a total volume of 25 µl. Cycling included one step of 95°C for 2 min, followed by 35 cycles of 95°C for 30 sec, 55°C for 30 sec, and 72°C for 90 sec, finished with one cycle at 72°C for 5 min (Skantar et al., 2007). 28S: Amplification of the 28S large ribosomal subunit (LSU) D2-D3 expansion segment included the primers D2A [5′-ACAAGTACCGTGAGGGAAAGTT-3′] and D3B

Andrea M. Skantar, Zafar A. Handoo, Mihail R. Kantor, Lynn K. Carta, Jamal Faghihi, Virginia Ferris

Journal of Nematology, Volume 52 , 1–8

research-article | 30-November-2019

Molecular and morphological characterization of the amaryllis lesion nematode, Pratylenchus hippeastri (Inserra et al., 2007), from California

oxidase I (COI) was amplified with JB3 [5’-TTTTTTGGGCATCCTGAGGTTTAT-3’] and JB5 [5’-AGCACCTAAACTTAA AACATAATGAAAATG-3’] (Derycke et al., 2005) as described in Ozbayrak et al. (2019). PCR amplicons of 403 bp were cleaned and sequenced directly with the same primers. The 28 S large ribosomal subunit D2-D3 expansion segment was obtained via amplification with the primers D2A [5’-ACAAGTACCGTGAGGGAAAGTTG-3’] and D3B [5’-TCGGAAGGAACCAGCTACTA-3’] (De Ley et al., 2005; Ye et al., 2007), producing sequences of

Zafar A. Handoo, Andrea M. Skantar, Mihail R. Kantor, Saad L. Hafez, Maria N. Hult

Journal of Nematology, Volume 52 , 1–5

research-article | 26-March-2021

First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae

these worms between genera and within the family Anisakidae (Nadler et al., 2005, 2007). On the contrary, high copy and short transcribed rDNA spacers ITS1, ITS2 (Campbell et al., 1994; Hoste et al., 1995; Samson-Himmelstjerna et al., 1997) and the region spanning the ITS1, the 5.8S gene, and the ITS2 of the ribosomal DNA are suitable genetic markers for the identification of nematodes, in particular anisakid species regardless of their stage of development (D’Amelio et al., 2000; Jabbar et al

Alina E. Safonova, Anastasia N. Voronova, Konstantin S. Vainutis

Journal of Nematology, Volume 53 , 1–10

research-article | 06-June-2019

Phylogenetic studies on three Helicotylenchus species based on 28S rDNA and mtCOI sequence data

process as well as to delineate more phylogenetically distant species, which share the same morphology (cryptic species), the support of a molecular approach is needed. To date, only about 20% of Helicotylenchus species has been molecularly characterized using mostly ribosomal DNA fragments (18S, ITS, 28S rDNA; GenBank resources). Mitochondrial cytochrome c oxidase subunit I (mtCOI) sequences were reported for one species, the recently described H. oleae (Palomares-Rius et al., 2018), and a cytochrome

K. Rybarczyk-Mydłowska, E. Dmowska, K. Kowalewska

Journal of Nematology, Volume 51 , 1–17

research-article | 30-November-2019

Description of Deladenus brevis n. sp. (Sphaerularioidea: Neotylenchidae) from Iran: a morphological and molecular phylogenetic study

Fariba Heydari, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–13

research-article | 30-November-2019

Molecular identification of Bursaphelenchus cocophilus associated to oil palm (Elaeis guineensis) crops in Tibu (North Santander, Colombia)

, covered vulva by a protuding anterior vulvar lip, presence of a long post-uterine sac, a narrow and elongated tail with a clindroid and rounded terminus (Opperman, 1995). Currently, DNA sequences of segment D2-D3, Internal Transcribed Spacer-(ITS) of ribosomal RNA and Cytochrome oxidase subunit I-COI of mitochondrial DNA have been obtained for B. cocophilus in coconut palm and deposited in public molecular databases, which are used as a reference for the identification of the nematode (Ye et al., 2007

Greicy Andrea Sarria, Donald Riascos-Ortiz, Hector Camilo Medina, Yuri Mestizo, Gerardo Lizarazo, Francia Varón De Agudelo

Journal of Nematology, Volume 52 , 1–12

original-paper | 28-March-2019

Predominance of Lactobacillus plantarum Strains in Peruvian Amazonian Fruits

, the API 50CHL (Biomerieux, Marcy l’etoile, France) kit, which tests for 49 carbohydrates and esculin. Other systems designed for Gram-positive or Gram-negative bacteria have been applied to LAB identification, such as the Biolog system, which includes the fermentation of 96 carbohydrates (Moraes et al. 2013). On the other hand, the development of molecular techniques has allowed more accurate identification of LAB. The wide method used for this purpose is based on ribosomal gene sequencing or


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 127–137

original-paper | 17-September-2021

Characteristics of Vaginal Microbiome in Women with Pelvic Inflammatory Disease in Korea


Polish Journal of Microbiology, Volume 70 , ISSUE 3, 345–357

research-article | 30-November-2019

Oscheius myriophila (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

, 10 females and 10 males were observed under a microscope (Nikon eclipse e200), and morphometric measurements were performed (Table 1). The maximum and minimum ranges of the body length were obtained: L, body width: W, spicules, distance from the part before the vulva, and length of eggs. The average values were calculated. Molecular identification For molecular identification, an 832 bp fragment of the D2D3 region of the 28s ribosomal gene was amplified by PCR. The sequence of the MC5 2014

Iveth del Rocio Castro-Ortega, Juan Manuel Caspeta-Mandujano, Ramón Suárez-Rodríguez, Guadalupe Peña-Chora, José Augusto Ramírez-Trujillo, Karina Cruz-Pérez, Iván Arenas Sosa, Víctor Manuel Hernández–Velázquez

Journal of Nematology, Volume 52 , 1–8

original-paper | 19-March-2021

A Clinical Trial to Evaluate the Efficacy of α-Viniferin in Staphylococcus aureus – Specific Decolonization without Depleting the Normal Microbiota of Nares

ribosomal RNA gene. We used the V4 region-specific primers with a locus-specific overhang sequence: 515F-5’ – TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-GTGCCAGCMGCCGCGGTAA and 806R – 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-GGACTACHVGGGTWTCTAAT (overhang sequence-locus specific-sequence). We attached indexes and Illumina sequencing adapters through index PCR using a Nextera XT Index kit v2 (Illumina, USA). We used Agencourt AMPure XP (Beckman Coulter, USA) beads after every PCR step to purify the PCR products and


Polish Journal of Microbiology, Volume 70 , ISSUE 1, 117–130

research-article | 30-November-2020

Laimaphelenchus africanus n. sp. (Tylenchomorpha: Aphelenchoididae) from South Africa, a morphological and molecular phylogenetic study, with an update to the diagnostics of the genus

. The specimens were then transferred to two individual Eppendorf tubes containing 15 µl ddH2O and their respective DNA was extracted using the chelex-100 protocol of Rashidifard et al. (2019). The DNA samples were stored at –20°C until used for amplification. The partial sequences of the large subunit ribosomal DNA (LSU rDNA D2-D3) were amplified using forward primer D2A (5′–ACAAGTACCGTGAGGGAAAGT–3′) and reverse primer D3B (5′–TCGGAAGGAACCAGCTACTA–3′) (Nunn, 1992). The polymerase chain reaction (PCR

Farahnaz Jahanshahi Afshar, Milad Rashidifard, Joaquín Abolafia, Miloslav Zouhar, Hendrika Fourie, Majid Pedram

Journal Of Nematology, Volume 53 , 1–14

Research Article | 17-October-2018

Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination

morphometric data ranges. The morphological features and morphometrics of the second studied species, A. helicus, agreed well with the data given for the type population. However, detailed study of fresh females revealed it has three drop-shaped stylet knobs and long PUS, making it typologically similar to the genus Robustodorus, meriting its taxonomic revision, i.e., transferring to it. In molecular phylogenetic analyses using partial small and large subunit ribosomal RNA gene (SSU and LSU rDNA) sequences

Farzad Aliramaji, Ebrahim Pourjam, Sergio Álvarez-Ortega, Farahnaz Jahanshahi Afshar, Majid Pedram

Journal of Nematology, Volume 50 , ISSUE 3, 437–452

research-article | 30-November-2020

Morphological and molecular characterization of Filenchus pseudodiscus n. sp. from east Golestan province, north Iran; with an updated phylogeny of Malenchus Andrássy, 1968 (Tylenchomorpha: Tylenchidae)

ribosomal markers (e.g. Panahandeh et al., 2018; Qing and Bert, 2018), the two currently resolved phylogenies using both SSU and LSU ribosomal markers, show the genus Filenchus is polyphyletic. These results are updates to the study of Atighi et al. (2013), showing Filenchus is polyphyletic using SSU, but monophyletic using LSU data. Currently there are six species under the genus Discotylenchus (Geraert, 2008) all of which being established based upon morphological criteria. The SEM data are however

Parnaz Mortazavi, Fariba Heydari, Joaquín Abolafia, Pablo Castillo, Majid Pedram

Journal of Nematology, Volume 53 , 1–14

research-article | 30-November-2020

Characterization of Globodera ellingtonae Populations from Chile Utilizing Whole Genome Sequencing

C.N. Hesse, I. Moreno, O. Acevedo Pardo, H. Pacheco Fuentes, E. Grenier, L. M. Dandurand, I. A. Zasada

Journal of Nematology, Volume 53 , 1–9

research-article | 30-November-2020

An interesting rare tylenchid species, Antarctenchus urmiensis n. sp. (Tylenchomorpha; Psilenchidae) from Urmia Lake islands, northwest Iran, with a discussion on the taxonomy of related genera

Mohammad Amiri Bonab, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 53 , 1–14

Research Article | 26-September-2018

First Report of Cactodera estonica in Canada

consistent with those of C. estonica, for which the elongated cyst and short hyaline in J2 are characteristic for the species. Ribosomal DNA of the ITS, 18S, and D2/D3 of 28S regions were PCR amplified from cysts and J2s using primers 18S (5′-TTGATTACGTCCCTGCCCTTT-3′) and 26S (5′-TTTCACTCGCCGTTACTAAGG-3′) (Vrain et al., 1992), D2A (5′-ACAAGTACCGTGAGGGAAAGT-3′) (Nunn, 1992) and D3B (5′-GACCCGTCTTGAAACACGGA-3′) (De Ley et al., 1999), and sequenced. The sequences of the ITS and D2/D3 regions of 1,480 and


Journal of Nematology, Volume 49 , ISSUE 4, 403–403

Article | 01-July-2019


well as from ATP by the RelA and SpoT enzymes, in response to a deficiency in nutritional substances. As a result of these reactions, AMP is also produced. Phosphate groups marked in grey are present only in the case of GTP and pppGpp. Alarmones influence the metabolism of a bacterial cell, i.a., by lowering the transcription of genes encoding rRNA, tRNA and ribosomal proteins and increasing the expression of those encoding proteins involved in the synthesis of amino acids and the response to

Julia Berdychowska, Justyna Boniecka, Grażyna B. Dąbrowska

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 2, 127–142

Article | 24-July-2017

First Report of the Fig Cyst Nematode, Heterodera fici Kirjanova, on Fig Tree, Ficus carica, in Ontario, Canada

National collection of Insects, Arachnids, and Nematodes (Accession no. 14851 to 14853 for the second stage juveniles and 14854–14855 for the cyst cones). For molecular analysis,DNA was extracted from individual juvenile (n = 4) from different cysts. A 1,151-bp fragment of ribosomal DNA containing ITS1-5.8SITS2 region was amplified and sequenced using primers 18S (5'-TTGATTACGTCCCTGCCCTTT-3') and 26S (5'- TTTCACTCGCCGTTACTAAGG-3') (Vrain et al., 1992). The sequence was


Journal of Nematology, Volume 49 , ISSUE 2, 131–132

research-article | 26-April-2019

First report of Meloidogyne javanica on Ginger and Turmeric in the United States

Abolfazl Hajihassani, Weimin Ye, Brooke B. Hampton

Journal of Nematology, Volume 51 , 1–3

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