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  • Journal Of Nematology

 

research-article | 30-November-2020

Virulence of Steinernema ceratophorum against different pest insects and their potential for in vivo and in vitro culture

clone the ITS1-5.8S-ITS2 rRNA gene, and D2-D3 expansion segment of the 28 S rRNA gene, respectively (Nguyen et al., 2006). The nematode was identified as Steinernema ceratophorum Jian, Reid and Hunt. IJ of S. ceratophorum were reared in last instar larvae of the greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae), and harvested by using White traps (Kaya and Stock, 1997). IJ suspension was concentrated to 2,000 IJ/ml, stored at 15°C, 100 rpm shaker and used within 2 weeks. Bioassays

Xun Yan, Guimei Chen, Yuqing Chen, Bingjiao Sun, Xinghui Gu, Weibing Ruan, Richou Han

Journal of Nematology, Volume 53 , 1–12

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