Review | 01-December-2019
Adsorption studies are usually required to confirm or rule out the presence of underlying alloantibodies in samples containing warm autoantibody. Allogeneic adsorptions are necessary if the patient has been recently transfused. Most commonly, allogeneic adsorptions are performed using a trio of phenotyped reagent red blood cells to rule out clinically significant alloantibodies to common antigens. The adsorbing cells may be used untreated or treated with enzymes or with ZZAP before adsorption
Christina Barron
Immunohematology, Volume 30 , ISSUE 4, 153–155
Article | 17-February-2021
blood cell (RBC) antibodies and make finding serologically compatible blood more difficult. Confirming the presence of HLA antibodies is complicated because HLA typing of reagent RBCs is often not reliable, HLA expression on RBCs is variable, and HLA antigens are not stable during storage.2 Having a method to rid a patient’s serum of these antibodies is beneficial for resolving suspected HLA antibody reactivity. Using platelets for the adsorption of HLA antibodies allows for the detection of
J. Jung,
C. Barron
Immunohematology, Volume 36 , ISSUE 1, 1–3
Article | 09-November-2020
The use of polyethylene glycol (PEG) to enhance the adsorption of warm autoantibodies on red blood cells (RBCs) was evaluated in our laboratory in an effort to reduce the time and cost associated with routine differential adsorptions. Sera from 19 patients with warm autoantibodies were tested. Fourteen of these sera contained alloantibodies or additional autoantibody specificities underlying the dominant autoantibody. The sera were differentially adsorbed using equal volumes of serum, reagent
Christina L. Barron,
Mary Beth Brown
Immunohematology, Volume 13 , ISSUE 4, 119–122
Article | 14-October-2020
Use of polyethylene glycol (PEG) to promote adsorption of autoantibodies is reported to give good recovery of concomitant alloantibodies. In initial experiments, PEG and ZZAP (Ficin and DTT) adsorption procedures were compared for removal of autoantibody and recovery of alloantibody. Postadsorption studies (n = 11) were performed and hemagglutination scores compared. In subsequent studies, equal volumes of alloantibody containing sera, PEG, and antigen-negative red blood cells (RBCs) were used
W. John Judd,
Louann Dake
Immunohematology, Volume 17 , ISSUE 3, 82–85
Article | 16-October-2019
, and adsorption with allogeneic RBCs—may be used to separate and properly identify antibodies that are present in a single serum or plasma sample.
Reagents/Supplies
Reagents
Supplies
Allogeneic red blood cells positive for antigen to which antibody is directed against, and antigen negative for other suspected antibody(ies)
Phosphate-buffered saline
Proteolytic enzyme
1 mL graduated, disposable pipettes
37°C incubator
Test tubes
Centrifuge
Procedural Steps
Select
E.M. Ekema
Immunohematology, Volume 33 , ISSUE 4, 155–158
Original Paper | 07-June-2016
This paper presents the interactions of Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas putida) bacteria with ceramic materials obtained by combustion synthesis. These studies were conducted based on an analysis of the adsorption of bacteria onto aggregates of ceramic materials in an aqueous suspension. The materials used in the studies were of a nanostructured nature and consisted mainly of carbides: silicon carbide (SiC) in the form of nanofibers (NFs) and nanorods (NRs
Andrzej Borkowski,
Filip Owczarek,
Mateusz Szala,
Marek Selwet
Polish Journal of Microbiology, Volume 65 , ISSUE 2, 161–170
Report | 14-March-2020
adsorbing time were observed. The modified-PEG adsorption is an improved method that may replace the current unenhanced method.
Mandy E. Etem,
Barbara Laird-Fryer,
Marie P. Holub,
John J. Hedl,
Daniel B. Symington,
Dolores Figueroa
Immunohematology, Volume 26 , ISSUE 3, 104–108
Article | 17-February-2021
two homologous RH genes: RHD and RHCE. The high degree of homology and opposite orientation of the two genes leads to the production of numerous Rh variants.2 D variants are usually classified as weak D, partial D, and DEL. Unlike weak D and partial D, DEL represents a weakened form of D that cannot be detected by conventional serology and requires the use of an adsorption-elution method; therefore, it is DEL (D-elute).3,4 The first examples of DEL+ RBCs were reported in the early 1980s when blood
R. Chaudhary,
S. Verma,
A. Verma
Immunohematology, Volume 36 , ISSUE 4, 133–136
Article | 16-October-2019
Cold-reactive autoagglutinins may mask the presence of underlying clinically significant alloantibodies. Adsorption with rabbit red blood cells (RBCs) or stroma can remove cold autoagglutinins found in the patient’s plasma/serum that are directed towards antigens expressed on the surface of rabbit RBCs. By removing these cold autoagglutinins, it is then possible to determine whether any underlying alloantibody reactivity is present. Although this method may also unintentionally adsorb
Adam Cobaugh
Immunohematology, Volume 34 , ISSUE 2, 46–48
Article | 28-August-2018
Granular activated carbon (GAC) is used for removing an excessive amount of organic contaminants causing undesirable taste, odour or colour of water, and refractive micropollutants, such as heavy metals or toxic organic compounds. However, adsorption is one of the most expensive unit processes used in treatment and renewal of water. This is due to the high cost of granular active carbon and the necessity of its frequent exchange or regeneration. In the paper the authors present the results of
Adam GUMIŃSKI,
Marcin KŁOS,
Jolanta GUMIŃSKA
Architecture, Civil Engineering, Environment, Volume 10 , ISSUE 4, 123–131
Report | 06-November-2019
This review updates new findings in drug-induced immunehemolytic anemia (DIIHA) since the 2007 review in Immunohematology by these authors. Twelve additional drugs have been added to the three tables listing drugs associated with drug-dependent antibodies, drugs associated with drug-independent antibodies, and drugs associated with nonimmunologic protein adsorption. Other updated findings include (1) piperacillin is currently the most commonly encountered cause of DIIHA, (2) new data on
George Garratty,
Patricia A. Arndt
Immunohematology, Volume 30 , ISSUE 2, 66–79
research-article | 30-November-2019
sufficiently low dew point temperature, other methods are occasionally used: gas heating, pressure reduction or injection of inhibitors that reduce the hydrate formation temperature. However, their effectiveness is much lower, so they are usually used only as ad-hoc methods [11, 12]. A number of methods of gas dehumidification are available worldwide, and one can distinguish between them:
– absorption methods
– adsorption methods
– recommending methods for cooling the gas stream [13].
The method of the
Natalia GENEROWICZ
Architecture, Civil Engineering, Environment, Volume 13 , ISSUE 3, 73–83
Article | 22-November-2020
Positive direct antiglobulin tests (DATs) associated with cephalosporin therapy have been reported, but rarely were associated with immune hemolytic anemia (IHA). In 1989, we described the first case of IHA associated with cefotetan (Cefotan™) causing hemolysis by the drug-adsorption mechanism. We now report the full details of our investigation. The patient was a 23-year-old female with a 2 1/2 year history of chronic ulcerative colitis. After 4 days of therapy with cefotetan (2 g/day
Robert J. Eckrich,
Susan Fox,
Delores Mallory
Immunohematology, Volume 10 , ISSUE 2, 51–54
Article | 30-November-2020
Two units of red blood cells (RBCs) were ordered for a 44-year-old Caucasian woman with renal failure and cancer. Pretransfnsion testing performed at the regional reference laboratory had revealed the presence of an antibody reactive with all cells at the indirect antiglobulin test (IAT) but apparently nonreactive by a prewarmed IAT. The patient’s RBCs were direct antiglobulin test negative. Adsorption of the serum with rabbit erythrocyte stroma or with allogeneic RBCs at 4°C reduced
Jill Storry,
Delores Mallory
Immunohematology, Volume 10 , ISSUE 3, 83–86
Article | 03-November-2017
doping gas concentration. The doping process is reversible, and the experimental results are interpreted in terms of adsorption and diffusion of the gas in the polymer, charge transfer, and transport of the injected carriers in the polymer chain. These experimental results and previous studies on other polymers and oxidizing gases demonstrate the potentiality of such materials to be used as sensitive part of gas sensors.
Allal LARBI,
Bachir DJEDOU,
Layachi BENNACER,
Mounir BOUSBIA-SALAH
International Journal on Smart Sensing and Intelligent Systems, Volume 2 , ISSUE 3, 448–462
Review | 01-December-2019
Patients demonstrating warm autoantibody specificity present serologic challenges for laboratory staff performing antibody identification in the blood bank. Autoantibody can be removed from plasma or serum by adsorption onto autologous red blood cells (RBCs) provided the patient has not been transfused in the previous 3 months. The adsorption process can be enhanced by enzyme pretreatment of autologous RBCs.
Farai Tsimba-Chitsva,
Susanne Bishop,
Kelly Kezeor
Immunohematology, Volume 28 , ISSUE 3, 88–90
Article | 01-June-2016
The accuracy of supercritical CO2 sorption determination on coal is important for estimating the CO2 sequestration potential of coal bed using CO2 injection into deep unminable coal seams. Based on the CO2 density change and CO2 sorption data of three selected coal samples, this paper investigates the effect of pressure-sensor accuracy on measurements of CO2 adsorption isotherms on coals with manometric equipment. The characteristic density-change of CO2 in proper pressure range determines a
Jiang Renxia,
Yu Hongguan,
Wang Li,
Chen Lihui
International Journal on Smart Sensing and Intelligent Systems, Volume 9 , ISSUE 2, 468–490
Report | 01-December-2019
Routine adsorption procedures to remove autoantibodies from patients’ serum often require many hours to perform. This timeconsuming process can create significant delays that affect patient care. This study modified the current adsorption method to reduce total adsorption time to 1 hour. A ratio of one part serum to three parts red blood cells (RBCs; 1:3 method) was maintained for all samples. The one part serum was split into three tubes. Each of these three aliquots of serum was mixed
J. Ryan Nobles,
Clare Wong
Immunohematology, Volume 29 , ISSUE 1, 5–10
Article | 28-April-2020
A recognized hazard of administering blood transfusions to patients with panreactive warm autoantibodies is that alloantibodies may be masked. Studies have shown the incidence of underlying alloantibodies to be 30 to 40 percent. Adsorption procedures can be used to remove autoantibodies and allow detection and identification of underlying alloantibodies. This study contains data from 126 patients referred to the Red Cell Immunohaematology laboratory at the National Blood Service, Newcastle upon
Martin Maley,
David G. Bruce,
Roderick G. Babb,
Angus W. Wells,
Mark Williams
Immunohematology, Volume 21 , ISSUE 3, 122–125
Research Article | 21-April-2017
Boron-containing lubricant for rail-curve lubrication is developed. Its effectiveness was proved by series of experiments carried out on different types of friction machines. The mechanism of antiscoring effect of boron-containing additives was analyzed in terms of physical adsorption.
Mikhail BOIKO,
Konstantin LEBEDINSKY
Transport Problems, Volume 10 , ISSUE 4, 99–105
Article | 10-November-2020
Testing of Ls(a+) and R1(a+) red cells with numerous antisera containing antibodies to low-incidence antigens indicated that these antigens are identical. This conclusion was confirmed by adsorption and elution tests, and supported by immunoblotting of Ls(a+) and R1(a+) cells with antibodies to giycophorin C and glycophorin D.
Leif Kornstad,
Carole Green,
Pertti Sistonen,
Geoff Daniels
Immunohematology, Volume 12 , ISSUE 1, 8–10
case-report | 25-June-2021
result between RBC and serum grouping tests. Subgroups of B are difficult to classify and include B3, Bx/Bweak (previously known as Bx), Bweak (previously known as Bm), and Bel phenotypes, according to the International Society of Blood Transfusion (ISBT).4,5 The B subgroups can be classified by their agglutination strength with anti-B, anti-A,B, and anti-H; the presence or absence of anti-B in the serum; the presence of B substance in saliva; the results of adsorption-elution testing; and molecular
E. Elardo,
N. Elbadri,
C. Sanchez,
V. Powell,
M. Smaris,
Y. Li,
J. Jacobson,
T. Hilbert,
T. Hamilton,
D.W. Wu
Immunohematology, Volume 37 , ISSUE 2, 89–94
Article | 15-February-2021
alloantibodies, due to drug-induced antibodies, or in the investigation of hemolytic disease of the fetus and newborn (HDFN).7 They may also be used in combination with in vitro adsorption studies to identify weakly expressed blood group antigens and for the identification of multiple alloantibodies.
Indications for heat elution include:
Investigation of ABO HDFN. Elution is rarely required, however, because the diagnosis is generally made from clinical findings consistent with HDFN in an ABO-incompatible
C. Dean-El,
N. Quraishy
Immunohematology, Volume 35 , ISSUE 2, 45–47
Research Article | 15-February-2020
An interdigitated microwave sensor was used to detect the adsorption of cytochrome c on hydroxyapatite thick films. Changes in the microwave spectral response were indicative of the presence of adsorbed cytochrome c. The sensitivity of the system was also evaluated using different protein loadings on the e films. The results suggest that the microwave sensor can be utilized to detect protein.
M. Salazar-Alvarez,
S.A.M. Tofail,
E. Magner,
O. Korostynska,
A. Mason,
A. Al-Shamma'a
International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–4
Case report | 27-December-2020
A patient whose red blood cells (RBCs) typed as Ge:2,3 produced an alloantibody to a high-frequency antigen in the Gerbich system. This antibody was shown to be nonreactive with Ge: -2, -3 RBCs using adsorption-elution studies. A monocyte monolayer assay (MMA) suggested that transfusion of Ge:2,3 RBCs to this patient would have reduced in vivo survival.
Michael I. Gorman,
Bobbye Woody
Immunohematology, Volume 5 , ISSUE 2, 55–57
Article | 26-August-2018
Krzysztof BARBUSIŃSKI,
Szymon SALWICZEK,
Agnieszka PASZEWSKA
Architecture, Civil Engineering, Environment, Volume 9 , ISSUE 2, 107–115
Article | 06-December-2020
The proposita was classified as Bel, By, or Bm, Le(b+) by routine blood grouping and by adsorption/elution studies using anti-A and -B hyperimmune pregnancy sera. Red cells from the proposita adsorbed as much anti-B from the hyperimmune sera as did red cells from normal B individuals, but adsorbed less anti-A,B (group O serum). Saliva contained H, but not B, soluble substance. Red cells from the proposita and a normal B donor were sensitized with monoclonal A and B blood group antibodies
Hans Erik Heier,
Leif Kornstad,
Ellen Namork,
Peder Østgard,
Randi Sandin
Immunohematology, Volume 8 , ISSUE 4, 94–99
Research Article | 15-February-2020
Important progress has been made towards the expanding of the limits of applicability of Fiber Bragg Grating optical sensors. The development of new thermostable coatings has opened up the possibility towards the use of this technology under extreme conditions, i.e. at high temperatures and pressures. The developed sensors have been tested for adsorption of a range of gaseous analytes and exhibit a sensitivity towards linear alkanes. Preliminary tests at high temperature in liquid phase
Dennis J. M. Snelders,
Arjen Boersma
International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–4
Article | 16-October-2019
Cold-reactive autoantibodies can mask the presence of underlying clinically significant alloantibodies in a patient’s plasma or serum. These autoantibodies are problematic when performing laboratory procedures such as ABO typing, red blood cell (RBC) crossmatching, antibody detection testing, and antibody identification. To avert the masking of clinically significant alloantibodies in a patient’s plasma or serum, adsorption studies can be performed at 4°C using autologous RBCs
Ernest M. Ekema
Immunohematology, Volume 34 , ISSUE 4, 158–160
Article | 15-February-2021
also allow for antigen typing of RBCs using indirect antiglobulin test (IAT) methods if the antigen is not destroyed by the reagent. Because ZZAP denatures many antigens determined in a common RBC phenotype (K, M, N, S, s, Fya, Fyb), this application has limited value. As a pretreatment before adsorption, ZZAP-treated RBCs may remove autoantibody more quickly and completely than untreated RBCs.
Lastly, ZZAP treatment can be helpful in complex antibody investigations. It can be used to create RBCs
S.I. Marckwardt
Immunohematology, Volume 35 , ISSUE 1, 9–10
Article | 16-October-2019
Chloroquine diphosphate (CDP) is a helpful tool in the blood bank for two main applications. The most common application is to render direct antiglobulin test–positive red blood cells (RBCs) free from membrane-bound IgG; these treated RBCs can then be used for autologous adsorption and/or to determine the patient’s RBC phenotype. Another common use of CDP is to remove human leukocyte antigens (HLAs) from RBCs to help identify or exclude the presence of antibodies to HLAs expressed
Thandar Aye,
Patricia A. Arndt
Immunohematology, Volume 34 , ISSUE 3, 98–102
Research Article | 12-December-2017
1-5 Napthalenedisulphonic acid (NDSA) doped polypyrrole thin films are prepared by chemical oxidation polymerization and applied as modified claddings to optical fibers for organophosphate (dimethyl-methyl phosphonate, DMMP) sensing. DMMP sensing takes place via intensity modulation in multimode fibers due to refractive index modulation in polypyrrole films due to DMMP adsorption. The polypyrrole films are characterized by FTIR and Raman spectroscopy. Ellipsometry and thin film transmission
Lalitkumar Bansal,
Mahmoud. A. El-Sherif
International Journal on Smart Sensing and Intelligent Systems, Volume 3 , ISSUE 2, 230–252
Article | 14-October-2020
showed that the mother’s serum contained anti-Mta . The father and all three children phenotyped as Mta+, while the mother was Mta–. Adsorption and elution experiments gave results which suggested that anti-Mta may be implicated in recurrent HDN in this family.
Carol C. Cheung,
Daniel Challis,
George Fisher,
Susan J. Russell,
Andrew Davis,
Hayley Bruce,
Julie Watt,
Beng H. Chong
Immunohematology, Volume 18 , ISSUE 2, 37–39
Article | 17-November-2020
and allogeneic adsorption. In 42 percent of the cases, all autoantibody reactivity was removed by both methods. No clinically significant alloantibodies were missed using serum dilution as compared to allogeneic adsorptions. We conclude that serum dilution is a simple, rapid way to initially assess tor the presence of alloantibodies that co-exist with autoantibodies.
Ragnhild Øyen,
Maria L. Angeles
Immunohematology, Volume 11 , ISSUE 3, 85–87
Article | 30-November-2020
An 80-year-old group O, D- (rr) female with anti-C, -D, -E, and -Fya received four units of crossmatch-compatible red blood cells (RBCs). The direct antiglobulin test (DAT) was negative. Two weeks later, jaundice, dark urine, a 16% drop in hematocrit (Hct), a 20% reticulocyte count, and absent haptoglobin occurred. During the next month, her DAT was positive with anti-IgG and -C3d. Acid eluates, which repeatedly showed anti-D specificity, were nonreactive with enzyme-treated D- RBCs. Adsorption
Walter H. Dzik,
Joyce Blank,
Paula Lutz,
Thomas G. Hirose,
Christine Lomas-Francis,
Marilyn Moulds
Immunohematology, Volume 10 , ISSUE 4, 117–119
Report | 06-November-2019
, such as which drugs most commonly cause DIIHA, the optimal testing methods for identifying them, and the theories behind the mechanisms by which they react. This article reviews the major changes in DIIHA since the early 1980s involving the immune complex mechanism, cephalosporins, nonimmunologic protein adsorption, and penicillins. Because serologic results associated with DIIHA can mimic those expected with autoimmune hemolytic anemia or hemolytic transfusion reactions, DIIHA may go undetected in
Patricia A. Arndt
Immunohematology, Volume 30 , ISSUE 2, 44–54
Article | 06-December-2020
During a serologic study on red cell samples from individuals representing three generations of a Chinese family, an unusual pattern of reactivity was noted in a sample from a daughter of an A1B2 individual. The results of direct ABO grouping, titration, and adsorption studies demonstrated that the red blood cells (RBCs) from the proposita and two of the proposita's uncles (1) expressed more B antigen than group B2 RBCs but less than group B1 RBCs; (2) expressed the B1 antigen but at a
Gongliang Zhang,
Yiging Wang,
Jie Zheng,
Alice Lee,
Robert J. Eckrich,
Delores M. Mallory,
Tsung Dao Lee
Immunohematology, Volume 9 , ISSUE 1, 11–14
Report | 06-November-2019
antibodies are investigated by testing drug-treated red blood cells (RBCs) or by testing RBCs in the presence of a solution of drug. Drug-independent antibodies are serologically indistinct from idiopathic warm autoantibodies and cannot be defined or excluded by serologic testing. Nonimmunologic protein adsorption, caused by some drugs, is independent of antibody production but may also cause immune hemolytic anemia. Serologic methods for testing for drug antibodies are presented, and observations from
Regina M. Leger,
Patricia A. Arndt,
George Garratty
Immunohematology, Volume 30 , ISSUE 2, 85–94
Research Article | 13-December-2017
) application. The ceramic sensors, based on sol gel thin film are shown to increase its capacitance upon water adsorption over the temperature range upto 250°C. The variation of capacity to voltage is shown to provide useful information on both break rate and location. The sensor installation spacing on the outer surface of the piping is determined as a function of the detection sensitivity. In this paper we have summarize the results of the development and characterization of ceramic humidity sensor for
Dilip Kumar Ghara,
Debdulal Saha,
Kamalendu Sengupta
International Journal on Smart Sensing and Intelligent Systems, Volume 1 , ISSUE 3, 784–798
case-report | 25-June-2021
R2R2 RBCs was nonreactive; a probable anti-f was then identified. Not all common alloantibodies could be excluded using the Jk(a–b–) RBCs, and, thus, differential adsorption studies with ZZAP (DTT-ficin)-modified allogeneic RBCs were performed to intentionally adsorb out the anti-Jk3. The adsorbed plasma revealed anti-Jka and anti-Jkb by LISS-IAT. Lastly, the second reference laboratory also identified an anti-P1 at room temperature with some reactivity carried through the IAT. The patient’s plasma
D.J.A.M. Talabong,
W.E. Kelley
Immunohematology, Volume 37 , ISSUE 2, 84–88
Article | 27-August-2018
Fluorine is a common chemical element. According to WHO guidelines, the F- ion content in drinking water cannot be higher than 1.5 mg/dm3. Excess of fluorine leads to many health problems: Alzheimer’s disease, neurological disorders or fluorosis (dental or skeletal). Fluoride can be removed from aqueous solutions by means of various methods (adsorption, precipitation, ion-exchange or membrane techniques). The aim of this paper was to evaluate the efficiency of electrodialysis in fluoride
Martyna GRZEGORZEK,
Katarzyna MAJEWSKA-NOWAK
Architecture, Civil Engineering, Environment, Volume 9 , ISSUE 4, 107–113
Article | 09-November-2020
of RBCbound C3b and C3d were relatively common and probably resulted from autoantibody activity, immune-complexes, and nonspecific adsorption. There was no association between positive ELDAT results and the presence of active hemolysis. The ELDAT was far more sensitive than the agglutination tests for detecting RBC-bound C3b and also for C3d if the monoclonal reagent was employed.
J.D. Bellamy,
D.J. Booker,
N.T. James,
R. Stamps,
R.J. Sokol
Immunohematology, Volume 13 , ISSUE 4, 123–131
case-report | 30-November-2020
patient’s RBC phenotype was found to be Le(a–b+) using anti-Lea and anti-Leb murine monoclonal antisera (Immucor, Norcross, GA), although the strength of reactivity was very weak (Fig. 2A). Secretor status was also performed by saliva inhibition study according to the AABB technical manual.6 The patient was a secretor of A and H substance, but the presence of A on RBCs could not be demonstrated by cold adsorption and heat elution methods, even after best efforts were tried, like increasing the cell-to
M.S. Bhagavathi,
N. Das,
S. Prakash,
A. Sahu,
S. Routray,
S. Mukherjee
Immunohematology, Volume 37 , ISSUE 4, 160–164
Article | 14-October-2020
out. Upon adsorption of the patient’s serum with ficin-treated Cde/Cde RBCs, both antibody specificities were adsorbed; however, the antibodies were not adsorbed with native (untreated) Cde/Cde RBCs. Furthermore, the autoantibody was not adsorbed by Rhnull cells, thereby suggesting Rh specificity. The serum was incompatible with cde/cde RBCs and compatible with Cde/Cde RBCs. The patient was successfully transfused with Cde/Cde RBCs followed by resolution of his anemia, as evidenced by an
Hsin-Yeh Hsieh,
Diana L. Moroney,
Deanne E. Naumann,
D. Jane Hata,
Nancy C. Vosnidou,
Rovenna L. Kessinger,
Nassir Shahab,
Nasrollah Hakami,
Daniel S. Smith
Immunohematology, Volume 18 , ISSUE 1, 19–22
Article | 18-October-2020
;) samples of Tanzanian origin were correctly typed and of 300 random donors of Caucasian origin with known Fy phenotype, only four out of 59 Fy(a+b–) donors showed the discrepant DNA-type Fy(a+b+). Serologic reinvestigation by adsorption and elution techniques confirmed weakly expressed Fyb antigen in these cases and DNA sequencing of the entire Duffy gene revealed identical point mutations in all of them. Specific PCR reactions were used to reinvestigate the C265T (Arg89Cys) and G298A (Ala100Thr
Christoph Gassner,
Richard L. Kraus,
Tadeja Dovc,
Susanne Kilga-Nogler,
Irene Utz,
Thomas Mueller,
Friedrich Schunter,
Diether Schoenitzer
Immunohematology, Volume 16 , ISSUE 2, 61–67
Article | 26-October-2020
transfusions. Red blood cells (RBCs) from the patient and her sister typed as Jk(a-b+) by direct hemagglutination and this phenotype was confirmed by negative adsorption and elution studies. Both infants' plasma samples were strongly reactive with 20 examples of Jk(a+) RBCs and nonreactive with 20 examples of Jk(a-) RBCs by SPRCA assays. Anti-Jka was not detected in either twins' plasma by indirect antiglobulin tests by tube method in low-ionic-strength saline solution or polyethylene glycol, or
Dawn H. Rumsey,
Sandra J. Nance,
Mary Rubino,
S. Gerald Sandler
Immunohematology, Volume 15 , ISSUE 4, 159–162
case-report | 25-June-2021
agglutination testing was performed to resolve the discrepancy. Antibody screening and identification showed panagglutination reactivity (Fig. 1B–D) with negative autocontrol (Bio-Rad, Hercules, CA); additional testing with anti-H lectin was negative, thus implying the mother’s blood group/type to be Oh D+. The antibody reacted strongly with cord group O RBCs (4+), similar to its reactivity with adult group O RBCs, thus ruling out anti-IH. However, we were not able to perform a complete adsorption of the
G. Mohan,
A. Vaidya,
S. Shastry
Immunohematology, Volume 37 , ISSUE 2, 59–63
original-report | 30-November-2020
K. Fennell,
M.A. Keller,
M.A. Villa,
C. Paccapelo,
M. Kucerakova,
J. Rosochova,
C. Clemente DosSantos,
L. Brackney,
C.J. Lee,
R. Metcalf,
G. Crovetti,
M. Barbieri,
S. Travali,
G. Barrotta,
G. Giuca,
L.E. Guerra,
G. Ochoa-Garay
Immunohematology, Volume 37 , ISSUE 4, 178–184
Article | 05-December-2017
than the loamy sand, which may have resulted in more adsorption of compounds in the root exudates that affect spore attachment. The components of the root exudates could have also been modified by soil type. The results of this study demonstrate that root exudates can decrease the attachment of P. penetrans endospores to root-knot nematodes, indicating that when these nematodes enter the root zone their susceptibility to spore attachment may decrease.
CHANG LIU,
PATRICIA TIMPER,
PINGSHENG JI,
TESFAMARIAM MEKETE,
SOUMI JOSEPH
Journal of Nematology, Volume 49 , ISSUE 3, 304–310
Review | 09-October-2019
DEL red blood cells (RBCs) type as D– by routine serologic methods and are transfused routinely, without being identified as expressing a very weak D antigen, to D– recipients. DEL RBCs are detected only by adsorption and elution of anti-D or by molecular methods. Most DEL phenotypes have been reported in population studies conducted in East Asia, although DEL phenotypes have been detected also among Caucasian individuals. Approximately 98 percent of DEL phenotypes in East Asians
Dong Hyang Kwon,
S. Gerald Sandler,
Willy Albert Flegel
Immunohematology, Volume 33 , ISSUE 3, 125–132
Case report | 01-December-2019
included autologous controls, direct antiglobulin test, elution, and titration of anti-D before and after adsorption of serum onto autologous RBCs. From molecular analyses, 459 individuals exhibited a weak D type. We described seven novel RHD variant alleles. The most frequent types of weak D were type 1 (30.1%), type 2 (23.7%), type 4.0 (10.2%), type 4.2.2 (20.3%), type 11 (3.9%), and type 15 (3.7%). Anti-D was identified in the sera of 9 of 47 individuals with weak D type 4.0, in 14 of 93 with weak D
Bach-Nga Pham,
Michèle Roussel,
Dominique Gien,
Maryline Ripaux,
Carine Auxerre,
Pierre-Yves Le Pennec,
Christine Andre-Botte
Immunohematology, Volume 29 , ISSUE 2, 55–62
Report | 01-December-2019
Anti-hrB (-RH31) and anti-HrB (-RH34) were found nearly 40 years ago in the serum of a South African woman. The anti-hrB was revealed after adsorption with DcE/DcE red blood cells (RBCs). Numerous anti-hrB, in the absence of anti-HrB, have since been identified. We obtained a sample of blood from this index case (Bastiaan) and report the molecular basis of her D+C–E–c+e+/–, V–VS+Hr+hrS+hrB–HrB– phenotype as well as results of testing her RBCs using currently
Marion E Reid,
Christine Halter Hipsky,
Randall W. Velliquette,
Christine Lomas-Francis,
Kathleen Larimore,
Coral Olsen
Immunohematology, Volume 28 , ISSUE 3, 97–103
Report | 14-March-2020
Anti-Ku seen in K0 (Kell-null) individuals has previously been shown to cause severe hemolytic transfusion reactions. Maternal anti-Ku can cause none or moderate to severe hemolytic disease of the fetus and newborn (HDFN). In two of four previously described HDFN cases, intrauterine transfusions were required because of severe anemia. We report a case in which maternal anti-Ku did not cause HDFN. Standard serologic methods were used for RBC antibody screening and identification, adsorption and
Ram M. Kakaiya,
Angelica Whaley,
Christine Howard-Menk,
Jigna Rami,
Mona Papari,
Sally Campbell-Lee,
Zbigniew Malecki
Immunohematology, Volume 26 , ISSUE 3, 119–122
Case report | 01-December-2019
%), 13 weak D type 3 (7.8%), and 2 weak D type 5 (1.2%) alleles were found. Among the partial D samples, 49 type 4.0 weak partial D (36%), 9 DAR (6.6%), 24 DFR (17.6%), 6 DBT (4.4%), 1 DHMi (0.73%), 26 DVI (19%), 14 DVa (10.3%), 5 DIVb (3.7%), and 2 DVII (1.5%) were observed. Two samples identified as DEL by adsorption-elution were characterized by molecular analyses as RHD(IVS5–38DEL4) and one sample was characterized as RHD(K409K). One sample was characterized as DHAR, a CE variant positive
Débora Castilho Credidio,
Jordão Pellegrino Jr.,
Lilian Castilho
Immunohematology, Volume 27 , ISSUE 1, 6–11
Original Paper | 04-September-2018
approximately 15% of DCF and 9% of SMX after 20 days of monosubstrate culture. However, a decrease in the optical density of the MG7 strain cultures was observed, caused by an insufficient carbon source for bacterial growth and proliferation. The adsorption of pharmaceuticals onto autoclaved cells was negligible, which confirmed that the tested strain was directly involved in the removal of DCF and SMX. In the presence of phenol as the additional carbon source, the MG7 strain was able to remove
ANNA WĘGRZYN,
EWA FELIS
Polish Journal of Microbiology, Volume 67 , ISSUE 3, 321–331