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Review | 01-December-2019

Allogeneic red blood cell adsorption for removal of warm autoantibody

Adsorption studies are usually required to confirm or rule out the presence of underlying alloantibodies in samples containing warm autoantibody. Allogeneic adsorptions are necessary if the patient has been recently transfused. Most commonly, allogeneic adsorptions are performed using a trio of phenotyped reagent red blood cells to rule out clinically significant alloantibodies to common antigens. The adsorbing cells may be used untreated or treated with enzymes or with ZZAP before adsorption

Christina Barron

Immunohematology, Volume 30 , ISSUE 4, 153–155

Article | 17-February-2021

Elimination of HLA antibodies by platelet adsorption

blood cell (RBC) antibodies and make finding serologically compatible blood more difficult. Confirming the presence of HLA antibodies is complicated because HLA typing of reagent RBCs is often not reliable, HLA expression on RBCs is variable, and HLA antigens are not stable during storage.2 Having a method to rid a patient’s serum of these antibodies is beneficial for resolving suspected HLA antibody reactivity. Using platelets for the adsorption of HLA antibodies allows for the detection of

J. Jung, C. Barron

Immunohematology, Volume 36 , ISSUE 1, 1–3

Article | 09-November-2020

The use of polyethylene glycol (PEG) to enhance the adsorption of autoantibodies

The use of polyethylene glycol (PEG) to enhance the adsorption of warm autoantibodies on red blood cells (RBCs) was evaluated in our laboratory in an effort to reduce the time and cost associated with routine differential adsorptions. Sera from 19 patients with warm autoantibodies were tested. Fourteen of these sera contained alloantibodies or additional autoantibody specificities underlying the dominant autoantibody. The sera were differentially adsorbed using equal volumes of serum, reagent

Christina L. Barron, Mary Beth Brown

Immunohematology, Volume 13 , ISSUE 4, 119–122

Article | 14-October-2020

PEG adsorption of autoantibodies causes loss of concomitant alloantibody

Use of polyethylene glycol (PEG) to promote adsorption of autoantibodies is reported to give good recovery of concomitant alloantibodies. In initial experiments, PEG and ZZAP (Ficin and DTT) adsorption procedures were compared for removal of autoantibody and recovery of alloantibody. Postadsorption studies (n = 11) were performed and hemagglutination scores compared. In subsequent studies, equal volumes of alloantibody containing sera, PEG, and antigen-negative red blood cells (RBCs) were used

W. John Judd, Louann Dake

Immunohematology, Volume 17 , ISSUE 3, 82–85

Article | 16-October-2019

Separation of multiple antibodies by adsorption with allogeneic red blood cells

, and adsorption with allogeneic RBCs—may be used to separate and properly identify antibodies that are present in a single serum or plasma sample. Reagents/Supplies Reagents Supplies Allogeneic red blood cells positive for antigen to which antibody is directed against, and antigen negative for other suspected antibody(ies) Phosphate-buffered saline Proteolytic enzyme 1 mL graduated, disposable pipettes 37°C incubator Test tubes Centrifuge Procedural Steps Select

E.M. Ekema

Immunohematology, Volume 33 , ISSUE 4, 155–158

Original Paper | 07-June-2016

Interaction of Gram-Positive and Gram-Negative Bacteria with Ceramic Nanomaterials Obtained by Combustion Synthesis – Adsorption and Cytotoxicity Studies

This paper presents the interactions of Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas putida) bacteria with ceramic materials obtained by combustion synthesis. These studies were conducted based on an analysis of the adsorption of bacteria onto aggregates of ceramic materials in an aqueous suspension. The materials used in the studies were of a nanostructured nature and consisted mainly of carbides: silicon carbide (SiC) in the form of nanofibers (NFs) and nanorods (NRs

Andrzej Borkowski, Filip Owczarek, Mateusz Szala, Marek Selwet

Polish Journal of Microbiology, Volume 65 , ISSUE 2, 161–170

Report | 14-March-2020

Allogeneic adsorptions: a comparison of the traditional method with a modified PEG adsorption method

adsorbing time were observed. The modified-PEG adsorption is an improved method that may replace the current unenhanced method.

Mandy E. Etem, Barbara Laird-Fryer, Marie P. Holub, John J. Hedl, Daniel B. Symington, Dolores Figueroa

Immunohematology, Volume 26 , ISSUE 3, 104–108

Article | 17-February-2021

Prevalence of DEL phenotype in D– blood donors in India

two homologous RH genes: RHD and RHCE. The high degree of homology and opposite orientation of the two genes leads to the production of numerous Rh variants.2 D variants are usually classified as weak D, partial D, and DEL. Unlike weak D and partial D, DEL represents a weakened form of D that cannot be detected by conventional serology and requires the use of an adsorption-elution method; therefore, it is DEL (D-elute).3,4 The first examples of DEL+ RBCs were reported in the early 1980s when blood

R. Chaudhary, S. Verma, A. Verma

Immunohematology, Volume 36 , ISSUE 4, 133–136

Article | 16-October-2019

Adsorption of cold agglutinins with rabbit red blood cells

Cold-reactive autoagglutinins may mask the presence of underlying clinically significant alloantibodies. Adsorption with rabbit red blood cells (RBCs) or stroma can remove cold autoagglutinins found in the patient’s plasma/serum that are directed towards antigens expressed on the surface of rabbit RBCs. By removing these cold autoagglutinins, it is then possible to determine whether any underlying alloantibody reactivity is present. Although this method may also unintentionally adsorb

Adam Cobaugh

Immunohematology, Volume 34 , ISSUE 2, 46–48

Article | 28-August-2018


Granular activated carbon (GAC) is used for removing an excessive amount of organic contaminants causing undesirable taste, odour or colour of water, and refractive micropollutants, such as heavy metals or toxic organic compounds. However, adsorption is one of the most expensive unit processes used in treatment and renewal of water. This is due to the high cost of granular active carbon and the necessity of its frequent exchange or regeneration. In the paper the authors present the results of


Architecture, Civil Engineering, Environment, Volume 10 , ISSUE 4, 123–131

Report | 06-November-2019

Drugs that have been shown to cause druginduced immune hemolytic anemia or positive direct antiglobulin tests: some interesting findings since 2007

This review updates new findings in drug-induced immunehemolytic anemia (DIIHA) since the 2007 review in Immunohematology by these authors. Twelve additional drugs  have been added to the three tables listing drugs associated with drug-dependent antibodies, drugs associated with drug-independent antibodies, and drugs associated with nonimmunologic protein adsorption. Other updated findings include (1) piperacillin is currently the most commonly encountered cause of DIIHA, (2) new data on

George Garratty, Patricia A. Arndt

Immunohematology, Volume 30 , ISSUE 2, 66–79

research-article | 30-November-2019


sufficiently low dew point temperature, other methods are occasionally used: gas heating, pressure reduction or injection of inhibitors that reduce the hydrate formation temperature. However, their effectiveness is much lower, so they are usually used only as ad-hoc methods [11, 12]. A number of methods of gas dehumidification are available worldwide, and one can distinguish between them: – absorption methods – adsorption methods – recommending methods for cooling the gas stream [13]. The method of the


Architecture, Civil Engineering, Environment, Volume 13 , ISSUE 3, 73–83

Article | 22-November-2020

Cefotetan-induced immune hemolytic anemia due to the drug-adsorption mechanism

Positive direct antiglobulin tests (DATs) associated with cephalosporin therapy have been reported, but rarely were associated with immune hemolytic anemia (IHA). In 1989, we described the first case of IHA associated with cefotetan (Cefotan™) causing hemolysis by the drug-adsorption mechanism. We now report the full details of our investigation. The patient was a 23-year-old female with a 2 1/2 year history of chronic ulcerative colitis. After 4 days of therapy with cefotetan (2 g/day

Robert J. Eckrich, Susan Fox, Delores Mallory

Immunohematology, Volume 10 , ISSUE 2, 51–54

Article | 30-November-2020

Misidentification of anti-Vel due to inappropriate use of prewarming and adsorption techniques

Two units of red blood cells (RBCs) were ordered for a 44-year-old Caucasian woman with renal failure and cancer. Pretransfnsion testing performed at the regional reference laboratory had revealed the presence of an antibody reactive with all cells at the indirect antiglobulin test (IAT) but apparently nonreactive by a prewarmed IAT. The patient’s RBCs were direct antiglobulin test negative. Adsorption of the serum with rabbit erythrocyte stroma or with allogeneic RBCs at 4°C reduced

Jill Storry, Delores Mallory

Immunohematology, Volume 10 , ISSUE 3, 83–86

Article | 03-November-2017


doping gas concentration. The doping process is reversible, and the experimental results are interpreted in terms of adsorption and diffusion of the gas in the polymer, charge transfer, and transport of the injected carriers in the polymer chain. These experimental results and previous studies on other polymers and oxidizing gases demonstrate the potentiality of such materials to be used as sensitive part of gas sensors.


International Journal on Smart Sensing and Intelligent Systems, Volume 2 , ISSUE 3, 448–462

Review | 01-December-2019

Warm autoadsorption with enzyme-treated red blood cells

Patients demonstrating warm autoantibody specificity present serologic challenges for laboratory staff performing antibody identification in the blood bank. Autoantibody can be removed from plasma or serum by adsorption onto autologous red blood cells (RBCs) provided the patient has not been transfused in the previous 3 months. The adsorption process can be enhanced by enzyme pretreatment of autologous RBCs.

Farai Tsimba-Chitsva, Susanne Bishop, Kelly Kezeor

Immunohematology, Volume 28 , ISSUE 3, 88–90

Article | 01-June-2016


The accuracy of supercritical CO2 sorption determination on coal is important for estimating the CO2 sequestration potential of coal bed using CO2 injection into deep unminable coal seams. Based on the CO2 density change and CO2 sorption data of three selected coal samples, this paper investigates the effect of pressure-sensor accuracy on measurements of CO2 adsorption isotherms on coals with manometric equipment. The characteristic density-change of CO2 in proper pressure range determines a

Jiang Renxia, Yu Hongguan, Wang Li, Chen Lihui

International Journal on Smart Sensing and Intelligent Systems, Volume 9 , ISSUE 2, 468–490

Report | 01-December-2019

Warm autoantibodies: time for a change

Routine adsorption procedures to remove autoantibodies from patients’ serum often require many hours to perform. This timeconsuming process can create significant delays that affect patient care. This study modified the current adsorption method to reduce total adsorption time to 1 hour. A ratio of one part serum to three parts red blood cells (RBCs; 1:3 method) was maintained for all samples. The one part serum was split into three tubes. Each of these three aliquots of serum was mixed

J. Ryan Nobles, Clare Wong

Immunohematology, Volume 29 , ISSUE 1, 5–10

Article | 28-April-2020

The incidence of red cell alloantibodies underlying panreactive warm autoantibodies

A recognized hazard of administering blood transfusions to patients with panreactive warm autoantibodies is that alloantibodies may be masked. Studies have shown the incidence of underlying alloantibodies to be 30 to 40 percent. Adsorption procedures can be used to remove autoantibodies and allow detection and identification of underlying alloantibodies. This study contains data from 126 patients referred to the Red Cell Immunohaematology laboratory at the National Blood Service, Newcastle upon

Martin Maley, David G. Bruce, Roderick G. Babb, Angus W. Wells, Mark Williams

Immunohematology, Volume 21 , ISSUE 3, 122–125

Research Article | 21-April-2017


Boron-containing lubricant for rail-curve lubrication is developed. Its effectiveness was proved by series of experiments carried out on different types of friction machines. The mechanism of antiscoring effect of boron-containing additives was analyzed in terms of physical adsorption.

Mikhail BOIKO, Konstantin LEBEDINSKY

Transport Problems, Volume 10 , ISSUE 4, 99–105

Article | 10-November-2020

Evidence that the low-incidence red cell antigens R1a and Lsa are identical

Testing of Ls(a+) and R1(a+) red cells with numerous antisera containing antibodies to low-incidence antigens indicated that these antigens are identical. This conclusion was confirmed by adsorption and elution tests, and supported by immunoblotting of Ls(a+) and R1(a+) cells with antibodies to giycophorin C and glycophorin D.

Leif Kornstad, Carole Green, Pertti Sistonen, Geoff Daniels

Immunohematology, Volume 12 , ISSUE 1, 8–10

case-report | 25-June-2021

B subgroup detection in a small hospital transfusion service

result between RBC and serum grouping tests. Subgroups of B are difficult to classify and include B3, Bx/Bweak (previously known as Bx), Bweak (previously known as Bm), and Bel phenotypes, according to the International Society of Blood Transfusion (ISBT).4,5 The B subgroups can be classified by their agglutination strength with anti-B, anti-A,B, and anti-H; the presence or absence of anti-B in the serum; the presence of B substance in saliva; the results of adsorption-elution testing; and molecular

E. Elardo, N. Elbadri, C. Sanchez, V. Powell, M. Smaris, Y. Li, J. Jacobson, T. Hilbert, T. Hamilton, D.W. Wu

Immunohematology, Volume 37 , ISSUE 2, 89–94

Article | 15-February-2021

Heat elution: a modification of the Landsteiner-Miller method

alloantibodies, due to drug-induced antibodies, or in the investigation of hemolytic disease of the fetus and newborn (HDFN).7 They may also be used in combination with in vitro adsorption studies to identify weakly expressed blood group antigens and for the identification of multiple alloantibodies. Indications for heat elution include: Investigation of ABO HDFN. Elution is rarely required, however, because the diagnosis is generally made from clinical findings consistent with HDFN in an ABO-incompatible

C. Dean-El, N. Quraishy

Immunohematology, Volume 35 , ISSUE 2, 45–47

Research Article | 15-February-2020

Detection of adsorbed cytochrome c on hydroxyapatite thick films using a microwave sensor

An interdigitated microwave sensor was used to detect the adsorption of cytochrome c on hydroxyapatite thick films. Changes in the microwave spectral response were indicative of the presence of adsorbed cytochrome c. The sensitivity of the system was also evaluated using different protein loadings on the e films. The results suggest that the microwave sensor can be utilized to detect protein.

M. Salazar-Alvarez, S.A.M. Tofail, E. Magner, O. Korostynska, A. Mason, A. Al-Shamma'a

International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–4

Case report | 27-December-2020

A case report: unusual Gerbich antibody in a patient with sickle cell anemia

A patient whose red blood cells (RBCs) typed as Ge:2,3 produced an alloantibody to a high-frequency antigen in the Gerbich system. This antibody was shown to be nonreactive with Ge: -2, -3 RBCs using adsorption-elution studies. A monocyte monolayer assay (MMA) suggested that transfusion of Ge:2,3 RBCs to this patient would have reduced in vivo survival.

Michael I. Gorman, Bobbye Woody

Immunohematology, Volume 5 , ISSUE 2, 55–57

Article | 26-August-2018



Architecture, Civil Engineering, Environment, Volume 9 , ISSUE 2, 107–115

Article | 06-December-2020

Expression of B and H antigens on red cells from a group Bweak individual studied by serologic and scanning electron microscopic techniques

The proposita was classified as Bel, By, or Bm, Le(b+) by routine blood grouping and by adsorption/elution studies using anti-A and -B hyperimmune pregnancy sera. Red cells from the proposita adsorbed as much anti-B from the hyperimmune sera as did red cells from normal B individuals, but adsorbed less anti-A,B (group O serum). Saliva contained H, but not B, soluble substance. Red cells from the proposita and a normal B donor were sensitized with monoclonal A and B blood group antibodies

Hans Erik Heier, Leif Kornstad, Ellen Namork, Peder Østgard, Randi Sandin

Immunohematology, Volume 8 , ISSUE 4, 94–99

Research Article | 15-February-2020

Development of thermostable FBG optical sensor for oil and gas applications

Important progress has been made towards the expanding of the limits of applicability of Fiber Bragg Grating optical sensors. The development of new thermostable coatings has opened up the possibility towards the use of this technology under extreme conditions, i.e. at high temperatures and pressures. The developed sensors have been tested for adsorption of a range of gaseous analytes and exhibit a sensitivity towards linear alkanes. Preliminary tests at high temperature in liquid phase

Dennis J. M. Snelders, Arjen Boersma

International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–4

Article | 16-October-2019

Cold autoadsorption

Cold-reactive autoantibodies can mask the presence of underlying clinically significant alloantibodies in a patient’s plasma or serum. These autoantibodies are problematic when performing laboratory procedures such as ABO typing, red blood cell (RBC) crossmatching, antibody detection testing, and antibody identification. To avert the masking of clinically significant alloantibodies in a patient’s plasma or serum, adsorption studies can be performed at 4°C using autologous RBCs

Ernest M. Ekema

Immunohematology, Volume 34 , ISSUE 4, 158–160

Article | 15-February-2021

ZZAP treatment of red blood cells

also allow for antigen typing of RBCs using indirect antiglobulin test (IAT) methods if the antigen is not destroyed by the reagent. Because ZZAP denatures many antigens determined in a common RBC phenotype (K, M, N, S, s, Fya, Fyb), this application has limited value. As a pretreatment before adsorption, ZZAP-treated RBCs may remove autoantibody more quickly and completely than untreated RBCs. Lastly, ZZAP treatment can be helpful in complex antibody investigations. It can be used to create RBCs

S.I. Marckwardt

Immunohematology, Volume 35 , ISSUE 1, 9–10

Article | 16-October-2019

Utility of chloroquine diphosphate in the blood bank laboratory

Chloroquine diphosphate (CDP) is a helpful tool in the blood bank for two main applications. The most common application is to render direct antiglobulin test–positive red blood cells (RBCs) free from membrane-bound IgG; these treated RBCs can then be used for autologous adsorption and/or to determine the patient’s RBC phenotype. Another common use of CDP is to remove human leukocyte antigens (HLAs) from RBCs to help identify or exclude the presence of antibodies to HLAs expressed

Thandar Aye, Patricia A. Arndt

Immunohematology, Volume 34 , ISSUE 3, 98–102

Research Article | 12-December-2017


1-5 Napthalenedisulphonic acid (NDSA) doped polypyrrole thin films are prepared by chemical oxidation polymerization and applied as modified claddings to optical fibers for organophosphate (dimethyl-methyl phosphonate, DMMP) sensing. DMMP sensing takes place via intensity modulation in multimode fibers due to refractive index modulation in polypyrrole films due to DMMP adsorption. The polypyrrole films are characterized by FTIR and Raman spectroscopy. Ellipsometry and thin film transmission

Lalitkumar Bansal, Mahmoud. A. El-Sherif

International Journal on Smart Sensing and Intelligent Systems, Volume 3 , ISSUE 2, 230–252

Article | 14-October-2020

Anti-Mta associated with three cases of hemolytic disease of the newborn

showed that the mother’s serum contained anti-Mta . The father and all three children phenotyped as Mta+, while the mother was Mta–. Adsorption and elution experiments gave results which suggested that anti-Mta may be implicated in recurrent HDN in this family.

Carol C. Cheung, Daniel Challis, George Fisher, Susan J. Russell, Andrew Davis, Hayley Bruce, Julie Watt, Beng H. Chong

Immunohematology, Volume 18 , ISSUE 2, 37–39

Article | 17-November-2020

A simple screening method to evaluate the presence of alloantibodies with concomitant warm autoantibodies

and allogeneic adsorption. In 42 percent of the cases, all autoantibody reactivity was removed by both methods. No clinically significant alloantibodies were missed using serum dilution as compared to allogeneic adsorptions. We conclude that serum dilution is a simple, rapid way to initially assess tor the presence of alloantibodies that co-exist with autoantibodies.  

Ragnhild Øyen, Maria L. Angeles

Immunohematology, Volume 11 , ISSUE 3, 85–87

Article | 30-November-2020

Autoimmune hemolysis following transfusion: a mimicking autoanti-D in a D- patient with alloanti-D

An 80-year-old group O, D- (rr) female with anti-C, -D, -E, and -Fya received four units of crossmatch-compatible red blood cells (RBCs). The direct antiglobulin test (DAT) was negative. Two weeks later, jaundice, dark urine, a 16% drop in hematocrit (Hct), a 20% reticulocyte count, and absent haptoglobin occurred. During the next month, her DAT was positive with anti-IgG and -C3d. Acid eluates, which repeatedly showed anti-D specificity, were nonreactive with enzyme-treated D- RBCs. Adsorption

Walter H. Dzik, Joyce Blank, Paula Lutz, Thomas G. Hirose, Christine Lomas-Francis, Marilyn Moulds

Immunohematology, Volume 10 , ISSUE 4, 117–119

Report | 06-November-2019

Drug-induced immune hemolytic anemia: the last 30 years  of changes

, such as which drugs most commonly cause DIIHA, the optimal testing methods for identifying them, and the theories behind the mechanisms by which they react. This article reviews the major changes in DIIHA since the early 1980s involving the immune complex mechanism, cephalosporins, nonimmunologic protein adsorption, and penicillins. Because serologic results associated with DIIHA can mimic those expected with autoimmune hemolytic anemia or hemolytic transfusion reactions, DIIHA may go undetected in

Patricia A. Arndt

Immunohematology, Volume 30 , ISSUE 2, 44–54

Article | 06-December-2020

A weak B antigen with serologic reactivity between B1 and B2 red blood cells found in a Chinese family

During a serologic study on red cell samples from individuals representing three generations of a Chinese family, an unusual pattern of reactivity was noted in a sample from a daughter of an A1B2 individual. The results of direct ABO grouping, titration, and adsorption studies demonstrated that the red blood cells (RBCs) from the proposita and two of the proposita's uncles (1) expressed more B antigen than group B2 RBCs but less than group B1 RBCs; (2) expressed the B1 antigen but at a

Gongliang Zhang, Yiging Wang, Jie Zheng, Alice Lee, Robert J. Eckrich, Delores M. Mallory, Tsung Dao Lee

Immunohematology, Volume 9 , ISSUE 1, 11–14

Report | 06-November-2019

How we investigate drug-induced immune hemolytic anemia

antibodies are investigated by testing drug-treated red blood cells (RBCs) or by testing RBCs in the presence of a solution of drug. Drug-independent antibodies are serologically indistinct from idiopathic warm autoantibodies and cannot be defined or excluded by serologic testing. Nonimmunologic protein adsorption, caused by some drugs, is independent of antibody production but may also cause immune hemolytic anemia. Serologic methods for testing for drug antibodies are presented, and observations from

Regina M. Leger, Patricia A. Arndt, George Garratty

Immunohematology, Volume 30 , ISSUE 2, 85–94

Research Article | 13-December-2017


) application. The ceramic sensors, based on sol gel thin film are shown to increase its capacitance upon water adsorption over the temperature range upto 250°C. The variation of capacity to voltage is shown to provide useful information on both break rate and location. The sensor installation spacing on the outer surface of the piping is determined as a function of the detection sensitivity. In this paper we have summarize the results of the development and characterization of ceramic humidity sensor for

Dilip Kumar Ghara, Debdulal Saha, Kamalendu Sengupta

International Journal on Smart Sensing and Intelligent Systems, Volume 1 , ISSUE 3, 784–798

case-report | 25-June-2021

A case series highlighting a common approach to identifying anti-Jk3

R2R2 RBCs was nonreactive; a probable anti-f was then identified. Not all common alloantibodies could be excluded using the Jk(a–b–) RBCs, and, thus, differential adsorption studies with ZZAP (DTT-ficin)-modified allogeneic RBCs were performed to intentionally adsorb out the anti-Jk3. The adsorbed plasma revealed anti-Jka and anti-Jkb by LISS-IAT. Lastly, the second reference laboratory also identified an anti-P1 at room temperature with some reactivity carried through the IAT. The patient’s plasma

D.J.A.M. Talabong, W.E. Kelley

Immunohematology, Volume 37 , ISSUE 2, 84–88

Article | 27-August-2018


Fluorine is a common chemical element. According to WHO guidelines, the F- ion content in drinking water cannot be higher than 1.5 mg/dm3. Excess of fluorine leads to many health problems: Alzheimer’s disease, neurological disorders or fluorosis (dental or skeletal). Fluoride can be removed from aqueous solutions by means of various methods (adsorption, precipitation, ion-exchange or membrane techniques). The aim of this paper was to evaluate the efficiency of electrodialysis in fluoride


Architecture, Civil Engineering, Environment, Volume 9 , ISSUE 4, 107–113

Article | 09-November-2020

Measurement of red blood cell-bound C3b and C3d using an enzyme-linked direct antiglobulin test

of RBCbound C3b and C3d were relatively common and probably resulted from autoantibody activity, immune-complexes, and nonspecific adsorption. There was no association between positive ELDAT results and the presence of active hemolysis. The ELDAT was far more sensitive than the agglutination tests for detecting RBC-bound C3b and also for C3d if the monoclonal reagent was employed.

J.D. Bellamy, D.J. Booker, N.T. James, R. Stamps, R.J. Sokol

Immunohematology, Volume 13 , ISSUE 4, 123–131

case-report | 30-November-2020

Blood group discrepancy in Ah para-Bombay phenotype: a rare blood group variant and its clinical significance

patient’s RBC phenotype was found to be Le(a–b+) using anti-Lea and anti-Leb murine monoclonal antisera (Immucor, Norcross, GA), although the strength of reactivity was very weak (Fig. 2A). Secretor status was also performed by saliva inhibition study according to the AABB technical manual.6 The patient was a secretor of A and H substance, but the presence of A on RBCs could not be demonstrated by cold adsorption and heat elution methods, even after best efforts were tried, like increasing the cell-to

M.S. Bhagavathi, N. Das, S. Prakash, A. Sahu, S. Routray, S. Mukherjee

Immunohematology, Volume 37 , ISSUE 4, 160–164

Article | 14-October-2020

Warm autoimmune hemolytic anemia with mimicking anti-c and -E specificities

out. Upon adsorption of the patient’s serum with ficin-treated Cde/Cde RBCs, both antibody specificities were adsorbed; however, the antibodies were not adsorbed with native (untreated) Cde/Cde RBCs. Furthermore, the autoantibody was not adsorbed by Rhnull cells, thereby suggesting Rh specificity. The serum was incompatible with cde/cde RBCs and compatible with Cde/Cde RBCs. The patient was successfully transfused with Cde/Cde RBCs followed by resolution of his anemia, as evidenced by an

Hsin-Yeh Hsieh, Diana L. Moroney, Deanne E. Naumann, D. Jane Hata, Nancy C. Vosnidou, Rovenna L. Kessinger, Nassir Shahab, Nasrollah Hakami, Daniel S. Smith

Immunohematology, Volume 18 , ISSUE 1, 19–22

Article | 18-October-2020

Fyx is associated with two missense point mutations in its gene and can be detected by PCR–SSP

;) samples of Tanzanian origin were correctly typed and of 300 random donors of Caucasian origin with known Fy phenotype, only four out of 59 Fy(a+b–) donors showed the discrepant DNA-type Fy(a+b+). Serologic reinvestigation by adsorption and elution techniques confirmed weakly expressed Fyb antigen in these cases and DNA sequencing of the entire Duffy gene revealed identical point mutations in all of them. Specific PCR reactions were used to reinvestigate the C265T (Arg89Cys) and G298A (Ala100Thr

Christoph Gassner, Richard L. Kraus, Tadeja Dovc, Susanne Kilga-Nogler, Irene Utz, Thomas Mueller, Friedrich Schunter, Diether Schoenitzer

Immunohematology, Volume 16 , ISSUE 2, 61–67

Article | 26-October-2020

Naturally-occurring anti-Jka in infant twins

transfusions. Red blood cells (RBCs) from the patient and her sister typed as Jk(a-b+) by direct hemagglutination and this phenotype was confirmed by negative adsorption and elution studies. Both infants' plasma samples were strongly reactive with 20 examples of Jk(a+) RBCs and nonreactive with 20 examples of Jk(a-) RBCs by SPRCA assays. Anti-Jka was not detected in either twins' plasma by indirect antiglobulin tests by tube method in low-ionic-strength saline solution or polyethylene glycol, or

Dawn H. Rumsey, Sandra J. Nance, Mary Rubino, S. Gerald Sandler

Immunohematology, Volume 15 , ISSUE 4, 159–162

case-report | 25-June-2021

Neonatal testing leading to the identification of Bh (para-Bombay) phenotype in the mother: case report with review of the literature

agglutination testing was performed to resolve the discrepancy. Antibody screening and identification showed panagglutination reactivity (Fig. 1B–D) with negative autocontrol (Bio-Rad, Hercules, CA); additional testing with anti-H lectin was negative, thus implying the mother’s blood group/type to be Oh D+. The antibody reacted strongly with cord group O RBCs (4+), similar to its reactivity with adult group O RBCs, thus ruling out anti-IH. However, we were not able to perform a complete adsorption of the

G. Mohan, A. Vaidya, S. Shastry

Immunohematology, Volume 37 , ISSUE 2, 59–63

original-report | 30-November-2020

New ABO intron 1 variant alleles

K. Fennell, M.A. Keller, M.A. Villa, C. Paccapelo, M. Kucerakova, J. Rosochova, C. Clemente DosSantos, L. Brackney, C.J. Lee, R. Metcalf, G. Crovetti, M. Barbieri, S. Travali, G. Barrotta, G. Giuca, L.E. Guerra, G. Ochoa-Garay

Immunohematology, Volume 37 , ISSUE 4, 178–184

Article | 05-December-2017

Influence of Root Exudates and Soil on Attachment of Pasteuria penetrans to Meloidogyne arenaria

than the loamy sand, which may have resulted in more adsorption of compounds in the root exudates that affect spore attachment. The components of the root exudates could have also been modified by soil type. The results of this study demonstrate that root exudates can decrease the attachment of P. penetrans endospores to root-knot nematodes, indicating that when these nematodes enter the root zone their susceptibility to spore attachment may decrease.


Journal of Nematology, Volume 49 , ISSUE 3, 304–310

Review | 09-October-2019

DEL phenotype

DEL red blood cells (RBCs) type as D– by routine serologic methods and are transfused routinely, without being identified as expressing a very weak D antigen, to D– recipients. DEL RBCs are detected only by adsorption and elution of anti-D or by molecular methods. Most DEL phenotypes have been reported in population studies conducted in East Asia, although DEL phenotypes have been detected also among Caucasian individuals. Approximately 98 percent of DEL phenotypes in East Asians

Dong Hyang Kwon, S. Gerald Sandler, Willy Albert Flegel

Immunohematology, Volume 33 , ISSUE 3, 125–132

Case report | 01-December-2019

Molecular analysis of patients with weak D and serologic analysis of those with anti-D (excluding type 1 and type 2)

included autologous controls, direct antiglobulin test, elution, and titration of anti-D before and after adsorption of serum onto autologous RBCs. From molecular analyses, 459 individuals exhibited a weak D type. We described seven novel RHD variant alleles. The most frequent types of weak D were type 1 (30.1%), type 2 (23.7%), type 4.0 (10.2%), type 4.2.2 (20.3%), type 11 (3.9%), and type 15 (3.7%). Anti-D was identified in the sera of 9 of 47 individuals with weak D type 4.0, in 14 of 93 with weak D

Bach-Nga Pham, Michèle Roussel, Dominique Gien, Maryline Ripaux, Carine Auxerre, Pierre-Yves Le Pennec, Christine Andre-Botte

Immunohematology, Volume 29 , ISSUE 2, 55–62

Report | 01-December-2019

Molecular background of RH in Bastiaan, the RH:–31,–34 index case, and two novel  RHD alleles

Anti-hrB (-RH31) and anti-HrB (-RH34) were found nearly 40 years ago in the serum of a South African woman. The anti-hrB was revealed after adsorption with DcE/DcE red blood cells (RBCs). Numerous anti-hrB, in the absence of anti-HrB, have since been identified. We obtained a sample of blood from this index case (Bastiaan) and report the molecular basis of her D+C–E–c+e+/–, V–VS+Hr+hrS+hrB–HrB– phenotype as well as results of testing her RBCs using currently

Marion E Reid, Christine Halter Hipsky, Randall W. Velliquette, Christine Lomas-Francis, Kathleen Larimore, Coral Olsen

Immunohematology, Volume 28 , ISSUE 3, 97–103

Report | 14-March-2020

Absence of hemolytic disease of fetus and newborn despite maternal high-titer IgG anti-Ku

Anti-Ku seen in K0 (Kell-null) individuals has previously been shown to cause severe hemolytic transfusion reactions. Maternal anti-Ku can cause none or moderate to severe hemolytic disease of the fetus and newborn (HDFN). In two of four previously described HDFN cases, intrauterine transfusions were required because of severe anemia. We report a case in which maternal anti-Ku did not cause HDFN. Standard serologic methods were used for RBC antibody screening and identification, adsorption and

Ram M. Kakaiya, Angelica Whaley, Christine Howard-Menk, Jigna Rami, Mona Papari, Sally Campbell-Lee, Zbigniew Malecki

Immunohematology, Volume 26 , ISSUE 3, 119–122

Case report | 01-December-2019

Serologic and molecular characterization of D variants in Brazilians: impact for typing and transfusion strategy

%), 13 weak D type 3 (7.8%), and 2 weak D type 5 (1.2%) alleles were found. Among the partial D samples, 49 type 4.0 weak partial D (36%), 9 DAR (6.6%), 24 DFR (17.6%), 6 DBT (4.4%), 1 DHMi (0.73%), 26 DVI (19%), 14 DVa (10.3%), 5 DIVb (3.7%), and 2 DVII (1.5%) were observed. Two samples identified as DEL by adsorption-elution were characterized by molecular analyses as RHD(IVS5–38DEL4) and one sample was characterized as RHD(K409K). One sample was characterized as DHAR, a CE variant positive

Débora Castilho Credidio, Jordão Pellegrino Jr., Lilian Castilho

Immunohematology, Volume 27 , ISSUE 1, 6–11

Original Paper | 04-September-2018

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

approximately 15% of DCF and 9% of SMX after 20 days of monosubstrate culture. However, a decrease in the optical density of the MG7 strain cultures was observed, caused by an insufficient carbon source for bacterial growth and proliferation. The adsorption of pharmaceuticals onto autoclaved cells was negligible, which confirmed that the tested strain was directly involved in the removal of DCF and SMX. In the presence of phenol as the additional carbon source, the MG7 strain was able to remove


Polish Journal of Microbiology, Volume 67 , ISSUE 3, 321–331

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