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  • Polish Journal Of Microbiology

 

original-paper | 19-March-2021

A Clinical Trial to Evaluate the Efficacy of α-Viniferin in Staphylococcus aureus – Specific Decolonization without Depleting the Normal Microbiota of Nares

mins employing a Hanil combi R515 (Hanil Scientific Inc., Korea) serially diluted the pellet 10-fold eight times using sterile distilled water. After that, we extracted DNA through mechanical lysis as described in the DNA Extraction section, amplified it by qRT-PCR, and used the data to create the standard curve in which each target CFU is plotted against the resulting Ct value. 16S rRNA-based amplicon sequencing. Following the DNA extraction, we amplified the V4 hyper-variable region of the 16S

MD ABDUR RAHIM, HOONHEE SEO, SUKYUNG KIM, YOON KYOUNG JEONG, HANIEH TAJDOZIAN, MIJUNG KIM, SAEBIM LEE, HO-YEON SONG

Polish Journal of Microbiology, Volume 70 , ISSUE 1, 117–130

original-paper | 05-December-2019

Illumina MiSeq Analysis and Comparison of Freshwater Microalgal Communities on Ulleungdo and Dokdo Islands

producer and consumer trophic levels (Berner 1992; Vitousek et al. 2002; Cardinale et al. 2011). However, microalgal community research based solely on the culturing faces certain limitations, particularly the difficulty in identifying and analyzing unculturable microorganisms (Handelsman 2004; Streit and Schmitz 2004). Consequently, amplicon sequencing analysis using Illumina MiSeq can be a powerful tool for the investigation of unculturable microorganisms in their natural environment (Knight 2000

HYUN-SIK YUN, YOUNG-SAENG KIM, HO-SUNG YOON

Polish Journal of Microbiology, Volume 68 , ISSUE 4, 527–539

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