disk diffusion” (Version 9.0, 2019) for the routine and extended internal quality control of antimicrobial susceptibility testing, the recommended control strains are with numbers from particular collections, e.g.: Escherichia coli ATCC 25922 (NCTC 12241, CIP 76.24, DSM 1103, CCUG 17620, CECT 434) .
The set of control strains for internal quality control should match the scope of diagnostics carried out by a laboratory, it should enable control of all stages of a laboratory’s routine work. The
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 205–211
Polish Journal of Microbiology , ISSUE 4, 517–524
the setting of ICU associated with limited therapeutic options (Vardakas et al. 2015).
Herein, we report an investigation of CRKP carriage and acquisition in the ICU to illustrate the clonal spread of CRKP isolates, their phenotypic and genotypic characteristics, and to track their evolutionary traits further.
Materials and Methods
CRKP isolates and Antimicrobial Susceptibility Testing. All the carbapenem-resistant K. pneumoniae strains were isolated from clinical specimens of the
Polish Journal of Microbiology , ISSUE 3, 311–319
atpD (ATP synthase beta chain), fusA (elongation factor G), glnS (glutaminyl-tRNA synthetase), gltB (glutamate synthase large subunit), gyrB (gyrase subunit B), infB (translation initiation factor IF-2), and ppsA (phosphoenolpyruvate synthase) were sequenced. The results obtained were compared with data at the Cronobacter PubMLST database.
Antimicrobial susceptibility testing. All Cronobacter strains were tested for susceptibility against 17 types of antibiotics, that were: ampicillin, aztreonam
Polish Journal of Microbiology , ISSUE 1, 5–14
Polish Journal of Microbiology , ISSUE 2, 225–232