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original-paper | 15-May-2020

Comparison of Rapid and Routine Methods of Identification and Antibiotic Susceptibility Testing of Microorganisms from Blood Culture Bottles

Introduction Bloodstream infections, especially in intensive care units, are among the most important causes of morbidity and mortality despite the antimicrobial treatment. The early diagnosis and treatment of these infections is clinically vital (Durmaz et al. 2003; Ferreira et al. 2011). For the proper identification and antibiotic susceptibility testing, the bacteria grown in blood culture bottles are sent to laboratories; the detection of bloodstream infections under the appropriate growth


Polish Journal of Microbiology, Volume 69 , ISSUE 2, 165–176

Original Paper | 09-March-2018

Detection of Acinetobacter spp. in Blood Cultures by an Improved Fluorescent in Situ Hybridization Assay

Fluorescent in situ hybridization (FISH) allows rapid detection of microorganisms. We aimed (i) to evaluate the sensitivity and specificity of FISH for the detection of Acinetobacter spp. in blood culture specimens and (ii) to test the simultaneous application of two genus-specific probes labeled with the same fluorochrome to increase the fluorescent signal intensity and improve the detection of Acinetobacter spp. Three hundred and twenty blood culture specimens were tested via both the

Hanieh Asaadi, Behrouz Naeimi, Somayyeh Gharibi, Abdalnaser Khosravi, Sina Dobaradaran, Reza Taherkhani, Saeed Tajbakhsh

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 3–10

Short Communication | 09-March-2018

Rapid Detection of Bloodstream Pathogens in Oncologic Patients with a FilmArray Multiplex PCR Assay: a Comparison with Culture Methods

The results of the FilmArray® Blood Culture Identification Panel (BCID) (BioFire Diagnostics) and the culture with susceptibility testing of 70 positive blood cultures from oncologic patients were compared. The multiplex PCR assay (BCID) identified 81 of the 83 isolates (97.6%), covered by the panel. The panel produced results in significantly shorter time than standard identification methods, when counted from receiving positive blood cultures bottles to the final results. It is an accurate

Maria Szymankiewicz, Beata Nakonowska

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 103–107

Original Paper | 10-December-2018

Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy

Abstract The gold standard in microbiological diagnostics of bacteremia is a blood culture in automated systems. This method may take several days and has low sensitivity. New screening methods that could quickly reveal the presence of bacteria would be extremely useful. The objective of this study was to estimate the effectiveness of these methods with respect to blood cultures in the context of antibiotic therapy. Blood samples from 92 children with sepsis were analyzed. Blood cultures were


Polish Journal of Microbiology, Volume 67 , ISSUE 4, 479–486

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