Report
The detection of polymorphism is the basis of blood group genotyping and phenotype prediction. Genotyping may be useful to determine blood groups when serologic results are unclear. The development and application of different methods for blood group genotyping may be needed as a substitute for blood group typing. The purpose of this study is to establish an approach for blood group genotyping based on a melting curve analysis of realtime polymerase chain reaction (PCR). Using DNA extracted
Tianxiang Gong,
Ying Hong,
Naihong Wang,
Xuemei Fu,
Changhua Zhou
Immunohematology, Volume 30 , ISSUE 4, 161–165
Article
Matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI-TOF MS), is a sensitive analytical method capable of resolving DNA fragments varying in mass by a single nucleotide. MALDI-TOF MS is applicable to blood group genotyping, as the majority of blood group antigens are encoded by single nucleotide polymorphisms. Blood group genotyping by MALDI-TOF MS can be performed using a panel (Hemo ID Blood Group Genotyping Panel, Agena Bioscience Inc., San Diego, CA) that is
Rhiannon S. McBean,
Catherine A. Hyland,
Robert L. Flower
Immunohematology, Volume 31 , ISSUE 2, 75–80
Case report
blood cell concentrates. This case demonstrates how the use of blood group genotyping in an acute setting can lead to a decrease in the unnecessary use of group O, D– blood products.
Joyce Curvers,
Volkher Scharnhorst,
Masja de Haas,
Loes Warnier-Wandel,
Daan van de Kerkhof
Immunohematology, Volume 28 , ISSUE 3, 85–87
Article
rare donor registries is required to obtain high-prevalence antigen-negative blood for transfusion. Several studies have compared molecular genotyping platforms with serologic phenotyping in patients and donors, with excellent concordance rates.16,19–21
Two PCR-based molecular genotyping platforms commercially available in the United States for blood group genotyping are the human erythrocyte antigen (HEA) PreciseType (formerly HEA BeadChip; Immucor, Norcross, GA) and ID CORE XT (Progenika-Grifols
C.A. Sheppard,
N.L. Bolen,
G. Meny,
M. Kalvelage,
G. Ochoa-Garay
Immunohematology, Volume 36 , ISSUE 4, 123–128
Report
The paucity of appropriate reagents for serologic typing of the Diego blood group antigens has prompted the development of a real-time PCR and melting curve analysis for Diego blood group genotyping. In this study, we phenotyped 4326 donor blood samples for Dia using semiautomated equipment. All 157 Di(a+) samples were then genotyped by PCR using sequence-specific primers (PCR-SSP) for DI*02 because of anti-Dib scarcity. Of the 4326 samples, we simultaneously tested 160 samples for Dia and Dib
Marcia C. Zago Novaretti,
Azulamara da Silva Ruiz,
Pedro Enrique Dorlhiac-Llacer,
Dalton Alencar Fisher Chamone
Immunohematology, Volume 26 , ISSUE 2, 66–70
Report
Connie M. Westhoff
Immunohematology, Volume 24 , ISSUE 4, 190–195
Article
The use of SNaPshot (Applied Biosystems, Foster City, CA) for predicting blood group antigens has emerged as an alternative to hemagglutination testing and also to the current low- and highthroughput blood group genotyping methods. Several groups have developed multiplex–polymerase chain reaction SNaPshot assays to determine single nucleotide polymorphisms (SNPs) in blood group genes with the purpose of identifying clinically relevant antigens and rare alleles. The selection of SNPs is
Flavia R.M. Latini,
Lilian M. Castilho
Immunohematology, Volume 31 , ISSUE 2, 53–57
Article
Marion E. Reid,
Maria J. Rios,
Kevin L. Cash,
Annie M. Strupp,
Joan M. Uehlinger
Immunohematology, Volume 15 , ISSUE 2, 61–65
Article
Barbera Veldhuisen,
C. Ellen van der Schoot,
Masja de Haas
Immunohematology, Volume 31 , ISSUE 2, 58–61
Report
Christine Lomas-Francis,
Helene DePalma
Immunohematology, Volume 24 , ISSUE 4, 180–190
Article
Gregory A. Denomme,
Michael J. Schanen
Immunohematology, Volume 31 , ISSUE 2, 69–74
Article
-genotype correlations were obtained. The potential use of the presented methods can be predicted in clinical transfusion medicine,allowing appropriate monitoring, early intervention, and improved care. When blood group genotyping techniques are necessary, this methodology is highly competitive for a routine laboratory.
Fernando Manuel Ferreira Araújo,
Christiana Pereira,
Fátima Monteiro,
Isabel Henriques,
Elsa Meireles,
Pedro Lacerda,
Ana Aleixo,
Regina Celeste,
Luis M. Cunha-Ribeiro,
Maria J. Rodrigues
Immunohematology, Volume 18 , ISSUE 3, 59–64
Report
Ricardo Omoto,
Marion E. Reid,
Lilian Castilho
Immunohematology, Volume 24 , ISSUE 4, 148–153