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original-paper

Comparison of Rapid and Routine Methods of Identification and Antibiotic Susceptibility Testing of Microorganisms from Blood Culture Bottles

Introduction Bloodstream infections, especially in intensive care units, are among the most important causes of morbidity and mortality despite the antimicrobial treatment. The early diagnosis and treatment of these infections is clinically vital (Durmaz et al. 2003; Ferreira et al. 2011). For the proper identification and antibiotic susceptibility testing, the bacteria grown in blood culture bottles are sent to laboratories; the detection of bloodstream infections under the appropriate growth

SADIK AKGUN, HAKAN SEZGIN SAYINER

Polish Journal of Microbiology , ISSUE 2, 165–176

Original Paper

Coomassie Blue G250 for Visualization of Active Bacteria from Lake Environment and Culture

fluorescent visualization of living, protein-rich bacteria, both in freshwater environment and culture. Our results revealed that the number of G250-stained bacteria from eutrophic lake was positively correlated with other standard bacterial activity markers, like number of bacteria containing 16S rRNA, bacterial secondary productionor maximal potential leucine-aminopeptidase activity. In case of the E. coli culture, the percentage of bacteria visualized with G250 was similar to that of bacteria which

Bartosz Kiersztyn, Waldemar Siuda, Ryszard Chróst

Polish Journal of Microbiology , ISSUE 3, 365–373

Original Paper

Detection of Acinetobacter spp. in Blood Cultures by an Improved Fluorescent in Situ Hybridization Assay

Fluorescent in situ hybridization (FISH) allows rapid detection of microorganisms. We aimed (i) to evaluate the sensitivity and specificity of FISH for the detection of Acinetobacter spp. in blood culture specimens and (ii) to test the simultaneous application of two genus-specific probes labeled with the same fluorochrome to increase the fluorescent signal intensity and improve the detection of Acinetobacter spp. Three hundred and twenty blood culture specimens were tested via both the

Hanieh Asaadi, Behrouz Naeimi, Somayyeh Gharibi, Abdalnaser Khosravi, Sina Dobaradaran, Reza Taherkhani, Saeed Tajbakhsh

Polish Journal of Microbiology , ISSUE 1, 3–10

Short Communication

Rapid Detection of Bloodstream Pathogens in Oncologic Patients with a FilmArray Multiplex PCR Assay: a Comparison with Culture Methods

The results of the FilmArray® Blood Culture Identification Panel (BCID) (BioFire Diagnostics) and the culture with susceptibility testing of 70 positive blood cultures from oncologic patients were compared. The multiplex PCR assay (BCID) identified 81 of the 83 isolates (97.6%), covered by the panel. The panel produced results in significantly shorter time than standard identification methods, when counted from receiving positive blood cultures bottles to the final results. It is an accurate

Maria Szymankiewicz, Beata Nakonowska

Polish Journal of Microbiology , ISSUE 1, 103–107

Short Communication

Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods

The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally

Agnieszka Figas, Magdalena Wieczorek, Bogumiła Litwińska, Włodzimierz Gut

Polish Journal of Microbiology , ISSUE 4, 479–483

Research Article

Enhanced entomopathogenic nematode yield and fitness via addition of pulverized insect powder to solid media

(Galleria mellonella, Tenebrio molitor, and Lucillia sericata) at three dose levels (1, 3, and 5%). The impact of insect powder was assessed on infective juvenile (IJ) yield in solid media. Additionally, IJs produced in solid culture were subsequently assessed for virulence, and progeny production in a target insect, Spodoptera litura. The dose level of larval powder had a significant effect on IJ yield in both trials, whereas insect type had significant effect on IJ yield in trial 1 but not in trial 2

Shiyu Zhen, Yang Li, Yanli Hou, Xinghui Gu, Limeng Zhang, Weibin Ruan, David Shapiro-Ilan

Journal of Nematology , ISSUE 4, 495–506

Original Paper

Levels of Organic Compounds, Number of Microorganisms and Cadmium Accumulation in Festuca ovina Hydroponic Culture

Małgorzata Majewska, Anna Słomka

Polish Journal of Microbiology , ISSUE 2, 191–200

Original Paper

A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic Microbes

microbes with high potential urease activity (> 10 μmol urea hydrolyzed per min per ml of enrichment culture) were repeatedly enriched under non-sterile conditions. In addition, the ureolytic enrichment obtained in this study showed in vitro calcium carbonate precipitation. Fluorescence in situ hybridization analysis showed the existence of bacteria of the phylum Firmicutes in the bioreactor system. Our data demonstrate that this DHS bioreactor system is a useful system for the enrichment and

Masataka Aoki, Takuya Noma, Hiroshi Yonemitsu, Nobuo Araki, Takashi Yamaguchi, Kazuyuki Hayashi

Polish Journal of Microbiology , ISSUE 1, 59–65

Original Paper

Suppressive Effect of Trichoderma spp. on toxigenic Fusarium species

cerealis, Fusarium culmorum, Fusarium graminearum and Fusarium temperatum). Dual-culture bioassay on potato dextrose agar (PDA) medium clearly documented that all of the Trichoderma strains used in the study were capable of influencing the mycelial growth of at least four of all five Fusarium species on the fourth day after co-inoculation, when there was the first apparent physical contact between antagonist and pathogen. The qualitative evalu­ation of the interaction between the colonies after 14

Lidia Błaszczyk, Aneta Basińska-Barczak, Hanna Ćwiek-Kupczyńska, Karolina Gromadzka, Delfina Popiel, Łukasz Stępień

Polish Journal of Microbiology , ISSUE 1, 85–100

Article

Shifting leadership out of the backyard: Expanding opportunities for women leading in higher education in the Solomon Islands

cultural context, the unique island of Santa Isabel in the Solomon Islands. Semi-structured interviews and focus group discussions were engaged to explore women’s leadership perceptions and experiences and how these ideas were realized in the way they practised leadership. Findings indicated that women’s perceptions of, and participation in, leadership was immersed in a cultural context which was founded on a belief of matrilineal leadership culture providing opportunities for women to have

Susanne Maezama

Journal of Educational Leadership, Policy and Practice , ISSUE 1, 51–64

Original Paper

Natural Attenuation Potential of Polychlorinated Biphenyl-Polluted Marine Sediments

petroleum hydrocarbons (TPHs). Then we adopted both culture-dependent and culture-independent (PCR-DGGE) approaches to identify bacterial inhabitants of the polluted marine sediments from Shuwaikh harbor. The chemical analysis revealed spatial variation among the sampling stations in terms of total amount of PCBs, TPHs and the PCB congener fingerprints. Moreover, in all analyzed sediments, the medium-chlorine PCB congeners were more abundant than the low-chlorine and high-chlorine counterparts. PCR-DGGE

Sarah Aldhafiri, Huda Mahmoud, Mohammed Al-Sarawi, Wael A. Ismail

Polish Journal of Microbiology , ISSUE 1, 37–48

Article

The importance of safe space and student voice in schools that serve minoritized learners

This article is based on an ethnography conducted over a six year period that used participant observation, photography, focus groups, and interviews to discover and describe the emergent school culture and the lived experiences of female secondary students in an all-girls college preparatory school. This article shares the story of a group of women educators who created a novel school culture, and the female students who meet them there, to disrupt and transform the dailiness of sexism, racism

Katherine Cumings Mansfield

Journal of Educational Leadership, Policy and Practice , ISSUE 1, 25–38

Short Communication

Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of

Beata Zimowska, Ewa Dorota Zalewska, Ewa Dorota Król, Agnieszka Furmańczyk

Polish Journal of Microbiology , ISSUE 2, 281–285

Original Paper

Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy

Abstract The gold standard in microbiological diagnostics of bacteremia is a blood culture in automated systems. This method may take several days and has low sensitivity. New screening methods that could quickly reveal the presence of bacteria would be extremely useful. The objective of this study was to estimate the effectiveness of these methods with respect to blood cultures in the context of antibiotic therapy. Blood samples from 92 children with sepsis were analyzed. Blood cultures were

TOMASZ W. ŹRÓDŁOWSKI, DANUTA JURKIEWICZ-BADACZ, AGNIESZKA SROKA-OLEKSIAK, DOMINIKA SALAMON, MAŁGORZATA BULANDA, TOMASZ GOSIEWSKI

Polish Journal of Microbiology , ISSUE 4, 479–486

Book Review

PATIENT SAFETY CULTURE: THEORY, METHODS, AND APPLICATION. PATRICK WATERSON (ED).

Adrian Smith

Journal of the Australasian Society of Aerospace Medicine , 28–28

Research Article

Emotion recognition from the eye region in children with and without Autism Spectrum Disorder in Arab and Scandinavian countries

Sanna Kuusikko-Gauffin, Sherin Elsheikh, Sven Bölte, Manal Omar, Geylan Riad, Hanna Ebeling, Arja Rautio, Irma Moilanen

Scandinavian Journal of Child and Adolescent Psychiatry and Psychology , ISSUE 4, 159–169

Original Paper

Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812

The nutritional requirements and environmental conditions for a submerged culture of Streptomyces sp. 8812 were determined. Batch and fed-batch Streptomyces sp. 8812 fermentations were conducted to obtain high activity of secondary metabolites. In the study several factors were examined for their influence on the biosynthesis of the active metabolites-7-hydroxy-6-oxo-2,3,4,6-tetrahydroisoquinoline-3-carboxyl acid (C10H9NO4) and N-acetyl-3,4-dihydroxy-l-phenylalanine (C11H13NO5): changes in

Aleksandra Rajnisz, Adam Guśpiel, Magdalena Postek, Joanna Ziemska, Anna Laskowska, Daniel Rabczenko, Jolanta Solecka

Polish Journal of Microbiology , ISSUE 1, 51–61

original-paper

A Current Microbiological Picture of Mycobacterium Isolates from Istanbul, Turkey

(EZN) (GBL Rose Biology Laboratory, Istanbul) method and examined microscopically under immersion oil. Isolation of Mycobacterium species. For all the samples, both liquid and solid media are used for culture. Therefore, any isolate growing either on one of these media were used in identification studies. In some patients, multiple specimens were submitted for culture, and multiple cultures were positive for growth. In those patients, the very first isolate was used in the study. For the liquid

BILGE SUMBUL, MEHMET ZIYA DOYMAZ

Polish Journal of Microbiology , ISSUE 2, 185–191

Article

Leading schooling in Aotearoa New Zealand: Understanding and supporting the weight of culture for Māori teachers

Toni Torepe, Angus Hikairo Macfarlane, Sonja Macfarlane, Jo Fletcher, Richard Manning

Journal of Educational Leadership, Policy and Practice , ISSUE 2, 48–59

original-paper

Oligotrophic Nitrification and Denitrification Bacterial Communities in a Constructed Sewage Treatment Ecosystem and Nitrogen Removal of Delftia tsuruhatensis NF4

nutritional composition of the culture medium is important for effectively maintaining the microorganisms in nature. It has been recently reported that medium components would functionally influence the growth of microbiome communities (Li et al. 2018). Studies have found that the genus could strongly be enriched within the intestinal bacterial communities by different nutritional environments (Flint et al. 2017). The oligotrophic heterotrophic nitrifiers and oligotrophic aerobic denitrifiers are mainly

RUILAN YANG, JING LI, LUYAO WEI-XIE, LIN SHAO

Polish Journal of Microbiology , ISSUE 1, 99–108

Original Paper

Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache

Salma Mukhtar, Ayesha Ishaq, Sara Hassan, Samina Mehnaz, Muhammad S. Mirza, Kauser A. Malik

Polish Journal of Microbiology , ISSUE 3, 353–364

Short Communication

Vital Staining of Bacteria by Sunset Yellow Pigment

In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture

Peng Gao, Chengming Sun, Yuxia Li, Xiaohua Zou, Xun Wu, Yan Ling, Caifu Luan, Huipeng Chen

Polish Journal of Microbiology , ISSUE 1, 113–117

original-paper

PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

and chemical analyses were performed to confirm that the selected Streptomyces strains can produce the antifungal compounds (cyps genes) under the culture condition. Experimental Materials and Methods Streptomyces strains and genomic DNA extraction. A total of 40 Streptomyces strains were obtained from the collection of the Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan. The selected strains were previously isolated from Cholistan desert of

NAILA NOUREEN, MOHSIN TASSAWAR CHEEMA, SUMAIRA ANWAR, SHAHIDA HASNAIN, IMRAN SAJID

Polish Journal of Microbiology , ISSUE 2, 139–149

Short Communication

Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014

A 4-year study (2011–2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were posi­tive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) were positive for enteroviruses using cell culture methods. The highest detection rate was observed in the summer and autumn. In total, 185 enteroviruses were identified by using neutralization test. Echovirus 6 and 30 were the most common (41.7% and 37.5% respectively

Magdalena Wieczorek, Agnieszka Figas, Arleta Krzysztoszek

Polish Journal of Microbiology , ISSUE 2, 231–235

Short Communication

Survival of Bacteria in Respiratory Protective Filters

The aim of this study was to estimate the susceptibility of Staphylococcus hominis bacteria to the bacteriostatic agent in respiratory pro­tective filters. Four types of filter media of different characteristics were tested. The number of bacteria was estimated by a culture-based method. It was proved that in treated filters the number of S. hominis was significantly reduced, even below the detection limit, while in untreated material bacteria were able to grow and multiply up to 100-fold

Ewa B. Miaśkiewicz-Pęska, Tomasz Słomczyński

Polish Journal of Microbiology , ISSUE 4, 475–477

original-paper

Effects of Sodium Tripolyphosphate on Oral Commensal and Pathogenic Bacteria

antimicrobial effect of polyP3 against the mixed-species consortium consisting of the four bacterial species. Experimental Materials and Methods Bacterial strains and culture condition. We used S. gordonii G9B, F. nucleatum ATCC 23726, P. gingivalis ATCC 53977 (previously designated A7A1-28), and P. intermedia ATCC 49046. Each bacterial strain was grown either in Brucella agar (Becton, Dickinson and Company, Sparks, MD, USA) supplemented with 5% sheep blood, 5 µg/ml hemin (Sigma Chemical Co., St. Louis

JI-HOI MOON, MI HEE NOH, EUN-YOUNG JANG, SEOK BIN YANG, SANG WOOK KANG, KYU HWAN KWACK, JAE-IN RYU, JIN-YONG LEE

Polish Journal of Microbiology , ISSUE 2, 263–268

original-paper

Cytokine Levels in the In Vitro Response of T Cells to Planktonic and Biofilm Corynebacterium amycolatum

their biochemical profile (APICoryne; bioMérieux, France). The phenotypic identification was followed by the analysis of the sequences of the specific fragment of the 16S rRNA gene (Genomed, Poland) (Drancourt et al. 2000; Bernard et al. 2002a; Fernández-Natal et al. 2008). Cell culture. Human T lymphocytes cell line (Jurkat, Clone E6-1 ATCC®TIB-152) was used in the study. Cells were cultured in RPMI 1640 culture medium (Sigma Aldrich, USA) containing 10% heat-inactivated fetal calf serum (FCS

ALINA OLENDER, AGNIESZKA BOGUT, AGNIESZKA MAGRYŚ, JACEK TABARKIEWICZ

Polish Journal of Microbiology , ISSUE 4, 457–464

Short Communication

Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic Y. enterocolitica in animal and food samples. In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the ail gene. Additionally, biochemical tests and RT-PCR showed that nine Y. enterocolitica isolates carried the ail-gene. Two Y. enterocolitica

MILENA A. STACHELSKA

Polish Journal of Microbiology , ISSUE 2, –

Short Communication

Susceptibility of Vascular Implants to Colonization in vitro by Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa

We compared association of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Enterococcus faecalis with nine vascular implants after co-culture. Vascular implants were composed of various materials such as warp knitted polyester (with or without gelatin and silver ions), expanded polytetrafluoroethylene and biological materials – surface treated porcine pericardial patch and Omniflow II. The lowest overall number of associated bacteria was detected for

Witold Woźniak, Aleksandra Kozińska, Piotr Ciostek, Izabela Sitkiewicz

Polish Journal of Microbiology , ISSUE 1, 125–129

Article

Strategic and Genetic Networking: Relational Endowment in a Local Cultural Offer

The local theatrical offer is the result of all the theatre companies which perform shows in each other’s venues. Theatre hospitality is an inherently relational phenomenon, and besides big national and international tours, it is an important part of the local cultural landscape. Aiming at contributing to the literature on network analysis applied to the inquiry on culture, the research adopts the network perspective to test hypotheses on companies’ relational behaviors and mechanisms of

Andrea Gallelli

Connections , ISSUE 1-2, 69–84

Article

CURVILINEAR STRUCTURAL ENVELOPS IN CURRENT ARCHITECTURE

how to build curvilinear surfaces with shot crete technology – The EMP in Seattle (2000), The City of Culture in Santiago de Compostela (2010) and The Museum of the History of Polish Jews in Warsaw (2014) will be presented. This study highlights issues of constructing curvilinear building envelops and shows new relationships between geometry and material which are different than before.

Krystyna JANUSZKIEWICZ

Architecture, Civil Engineering, Environment , ISSUE 1, 11–16

Research Article

NOCARDIA SPP. – CHARACTERISTICS, PATHOGENICITY, TREATMENT

after immunosupression therapy. Nocardia asteroides is responsible for the majority of infections in humans. Diagnostic methods include cell culture and PCR. The symptoms vary depending on the form of the illness. Cough and hemoptysis are characteristic for pulmonary nocardiosis, while abscesses are typical for the cutaneous form. When the illness spreads, the symptoms vary depending on the organ. The treatment of choice is sulfonamide.

Michał Wiciński, Mateusz Maciej Węclewicz, Bartosz Malinowski, Jarosław Żak, Elżbieta Grześk, Grzegorz Grześk, Kamil Leis

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 68–75

Research paper

Lin28 overexpression inhibits neurite outgrowth of primary cortical neurons in vitro

overexpression, which was confirmed immunocytochemically, led to neurite underdevelopment for all time points during culture. Specifically, Lin28-overexpressing cells displayed significantly fewer primary neurites and a decreased dendritic branching index, compared to GFP-expressing controls. Additionally, Lin28 overexpression in primary cortical neurons induced the expression of high mobility group AT-Hook 2 (HMGA2). Taken together, our study demonstrates that constitutive Lin28 expression disrupts cortical

Mohammad Iqbal Hossain Bhuiyan, Seong Yun Kim, Kyung-Ok Cho

Acta Neurobiologiae Experimentalis , ISSUE 4, 297–304

Original Paper

Screening and Identification of Yeasts Antagonistic to Pathogenic Fungi Show a Narrow Optimal pH Range for Antagonistic Activity

-sensitive strain of Saccharomyces cerevisiae and three pathogenic fungi. We demonstrate that some strains with antagonistic activity against these pathogenic fungi can be found in antagonist culture tests. These yeasts were identified as members of Trichosporon asahii, Candida stellimalicola, Wickerhamomyces anomalus, Ustilago esculenta, Aureobasidium pullulans, and Pichia kluyveri. The results indicated that the antagonistic activity of these killer yeasts has a narrow optimal pH range. Furthermore, we

Pei-Hua Chen, Jui-Yu Chou

Polish Journal of Microbiology , ISSUE 1, 101–106

Article

Research on the Application of Political Teaching Under the Network Platform

With the advent of the era of network, information technology has penetrated into many fields such as politics, economy, culture and so on. Human’s production mode, life style and learning way have changed greatly. The traditional educational mode has been unable to meet the diverse needs of students. Our educational reform and development are facing unprecedented challenges. The driving point of educational reform is to drive educational modernization with educational information. In this

Pan Yingli

International Journal of Advanced Network, Monitoring and Controls , ISSUE 2, 95–98

Research Article

Work-life balance and family friendly policies

hours (e.g. access to part-time work); paid and unpaid leave (e.g. parental leave); and access to childcare. It then considers the work-life literature related to two specific industries – the Australian public sector, and health and social services – to identify work-life issues and practices specific to each industry. We then conclude with a general discussion of challenges associated with the policy-practice gap, focusing particularly on work intensification and the role of organisational culture

Natalie Skinner, Janine Chapman

Evidence Base , ISSUE 4, 1–17

Original Paper

Biohydrogen Production by Antarctic Psychrotolerant Klebsiella sp. ABZ11

facultative anaerobic condition. The isolate is able to ferment glucose, fructose and sucrose with biohydrogen production rate and yield of 0.8 mol/l/h and 3.8 mol/g, respectively at 10 g/l glucose concentration. It also showed 74% carbohydrate uptake and 95% oxygen uptake ability, and a wide growth temperature range with optimum at 37°C. Klebsiella sp. ABZ11 has a short biohydrogen production lag phase, fast substrate uptake and is able to tolerate the presence of oxygen in the culture medium. Thus, the

ABDULLAHI MOHAMMED, MOHD FIRDAUS ABDUL-WAHAB, MAZLAN HASHIM, ABDUL HAFIDZ OMAR, MOHD NADZRI MD REBA, MOHD FARID MUHAMAD SAID, KAMARUZAMAN SOEED, SITI AISYAH ALIAS, JERZY SMYKLA, MUSTAPHA ABBA, ZAHARAH IBRAHIM

Polish Journal of Microbiology , ISSUE 3, 283–290

Original Paper

Enzymes Involved in Naproxen Degradation by Planococcus sp. S5

Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. Planococcus sp. S5 is a gram positive strain able to degrade naproxen in monosubstrate culture (27%). However, naproxen is not a suf­ficient growth substrate for this strain. In the presence of benzoate, 4-hydroxybenzoic acid

Danuta Wojcieszyńska, Dorota Domaradzka, Katarzyna Hupert-Kocurek, Urszula Guzik

Polish Journal of Microbiology , ISSUE 2, 177–182

Article

The Effect of Endophytic Fungi on Nematode Populations in Summer-dormant and Summer-active Tall Fescue

;cultivars, Kentucky 31 (common toxic) and Texoma MaxQ II (novel endophyte) and the Mediterranean cultivar Flecha MaxQ (novel endophyte). Endophyte-free plants of each cultivar were controls. Each cultivar 3 endophyte combination was randomly assigned to a control, low or high inoculation rate of a mixed nematode culture containing stunt nematodes (Tylenchorhynchus spp.), ring nematodes (Criconemella spp.), spiral nematodes (Helicotylenchus spp.), and lesion nematodes (Pratylenchus spp

JAMES K. ROGERS, NATHAN R. WALKER, CAROLYN A. YOUNG

Journal of Nematology , ISSUE 2, 87–94

Article

A New Zealand case study: What is happening to lead changes to effective co-teaching in flexible learning spaces?

team who changed the school culture from traditional one teacher per classroom settings to four to five teachers with approximately 105 students in flexible learning spaces. The principal and three members of the governing Board of Trustees of the school were interviewed. The study found that the leadership team had invested considerable time into sustained professional development in ways to effectively develop collaborative teaching communities within flexible learning spaces. The professional

Jo Fletcher, Julie Mackey, Letitia Fickel

Journal of Educational Leadership, Policy and Practice , ISSUE 1, 70–83

Original Paper

Characterization of Endolithic Culturable Microbial Communities in Carbonate Rocks from a Typical Karst Canyon in Guizhou (China)

The endolithic environment is a ubiquitous habitat for microorganisms and a critical interface between biology and geology. In this study, a culture-based method and the phylogenetic analysis based on 16S rRNA and internal transcribed spacer (ITS) sequences were used to investigate the diversity of endolithic bacteria and fungi in two main types of carbonate rocks (namely dolomite and limestone) from Nanjiang Canyon in Guizhou karst area, China. The results of bacterial diversity indicated that

Yuan Tang, Jian-Zhong Cheng, Bin Lian

Polish Journal of Microbiology , ISSUE 4, 413–423

Original Paper

Improved Production of Recombinant Human β-NGF in Escherichia coli – a Bioreactor Scale Study

Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in Escherichia coli in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further

ZAHRA HAJIHASSAN, POURIA GHOLAMI TILKO, SEYEDEH MAHDIEH SADAT

Polish Journal of Microbiology , ISSUE 3, 355–363

Original Paper

Comparison of the Photosensitivity of Biofilms of Different Genera of Cariogenic Bacteria in Tooth Slices

suspension. Then, tooth slices previously incubated for 24 hours with broth cultures of broth culture of the four cariogenic organisms were exposed to antimicrobial photosensitization. The control samples consisted of planktonic and sessile cells that were exposed to TBO alone, laser alone and the bacterial cells that were not treated with TBO or laser. The results showed significant reductions in the viability of S. mutans, L. casei and A. viscosus in both planktonic form (to 13%, 30%, and 55

HOMA DARMANI, KHITAM H. TAWALBEH, AHMAD S. AL-HIYASAT, MOHAMMAD-ALI AL-AKHRAS

Polish Journal of Microbiology , ISSUE 4, 455–462

Article

Dispositions of a responsible early childhood education leader: Voices from the field

acting as a critical friend. We argue that responsible leadership must be purposefully grown, developed and sustained across the culture of the ECE setting. Underpinning this understanding is the need for dedicated leadership professional development that supports emerging and current leaders, and their teams, to engage in robust collegial dialogue and reflective practice in terms of what it means to be a responsible leader.

Gwen Davitt, Debbie Ryder

Journal of Educational Leadership, Policy and Practice , ISSUE 1, 18–31

original-paper

Prevalence and Antimicrobial Properties of Lactic Acid Bacteria in Nigerian Women During the Menstrual Cycle

of the menstrual cycle and the antimicrobial effects of the isolated LAB. Therefore, this study aimed at determining the prevalence of LAB at different stages of the menstrual cycle in Nigerian women with their potential antimicrobial properties. Experimental Materials and Methods Pathogens. Ten clinical strains of uropathogens: Klebsiella spp., Staphylococcus spp. and Pseudomonas spp. (isolated from urine) were collected from the culture collection of the Medical Microbiological Laboratory of

FOLASHADE GRACE ADEOSHUN, WERNER RUPPITSCH, FRANZ ALLERBERGER, FUNMILOLA ABIDEMI AYENI

Polish Journal of Microbiology , ISSUE 2, 203–209

Article

USE OF COMPACT TRICKLE BED BIOREACTOR FOR THE PURIFICATION OF AIR FROM A VOC’s MIXTURE – PRELIMINARY RESEARCH

The study presents the preliminary results of research with the aim to test a Compact Trickle-Bed Bioreactor (CTBB) to the biopurification of air from VOC’s mixtures (ethyl alcohol, dimethyl sulphide, styrene). The experiments were performed using a 45 dm3 semi-industrial scale bioreactor in which gas and liquid phases flowed co-currently in downward direction through a bed made up of polyethylene rings. The bioreactor was inoculated with a co-culture of microorganisms including bacterial

Damian KASPERCZYK, Krzyszof BARBUSIŃSKI, Violetta KOZIK

Architecture, Civil Engineering, Environment , ISSUE 2, 137–143

original-paper

Prevalence of Multi-Drug Resistant Mycobacterium Tuberculosis in Khyber Pakhtunkhwa – A High Tuberculosis Endemic Area of Pakistan

information software and analyzed using SPSS V.15 (IBM, USA). Sensitivity and specificity were calculated using Medcalc software (https://www.medcalc.org). Results Among 5759 TB suspects, 1969 (34%) were culture-positive, 3121 (54%) were culture-negative, and 344 (6%) were contaminated. The proportion of TB was higher in females (39%) than males (29%), thus, a strong association was observed between the gender and tuberculosis disease (χ (3) = 68.2, p = 0.001). It was observed that the age group of 25

SAJID ALI, MUHAMMAD TAHIR KHAN, ANWAR SHEED KHAN, NOOR MOHAMMAD, MUHAMMAD MUMTAZ KHAN, SAJJAD AHMAD, SADIQ NOOR, ABDUL JABBAR, CANTILLON DAIRE, FARIHA HASSAN

Polish Journal of Microbiology , ISSUE 2, 133–137

Article

Characterization of Lilium longiflorum cv. ‘Nellie White’ Infection with Root-lesion Nematode Pratylenchus penetrans by Bright-field and Transmission Electron Microscopy

Lilium longiflorum cv. Nellie White, commonly known as Easter lily, is an important floral crop with an annual wholesale value of over $26 million in the United States. The root-lesion nematode, Pratylenchus penetrans, is a major pest of lily due to the significant root damage it causes. In this study, we investigated the cytological aspects of this plant–nematode interaction using bright-field and transmission electron microscopy. We took advantage of an in vitro culture method

PAULO VIEIRA, JOSEPH MOWERY, JAMES KILCREASE, JONATHAN D. EISENBACK, KATHRYN KAMO

Journal of Nematology , ISSUE 1, 2–11

Original Paper

Evaluation of Neisseria meningitidis Carriage with the Analysis of Serogroups, Genogroups and Clonal Complexes among Polish Soldiers

types and clonal complexes of the isolates detected among Polish soldiers. The study was conducted during winter seasons of 2015 and 2016 and involved 883 professional soldiers from the Armoured Brigade in Świętoszów, Poland. The material for testing were nasopharyngeal swabs obtained from study participants. The samples were tested using standard microbiological methods (culture, incubation, microscopy, biochemical and automated identification). N. meningitidis isolates were subjected to slide

KRZYSZTOF KORZENIEWSKI, MONIKA KONIOR

Polish Journal of Microbiology , ISSUE 4, 493–500

original-paper

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

of soil sample was added to the medium (50 ml) and incubated for one week in shaker adjusted at 50°C and at 250 rpm. After a week, this culture was used as inoculum and added to a freshly prepared minimal salt medium supplemented with petrol. The culture was incubated in incubator shaker under the same conditions. The enrichment process was repeated for six weeks. After enrichment, the serial dilutions were prepared and each dilution (100 μl) was spread on petrol supplemented MSM agar medium

FATIMA MUCCEE, SAMINA EJAZ

Polish Journal of Microbiology , ISSUE 2, 193–201

Research Article

Identification of HG Types of Soybean Cyst Nematode Heterodera glycines and Resistance Screening on Soybean Genotypes in Northeast China

which increased virulence on resistant cultivars occurred, and single-cyst cultures from each soil sample were maintained for more than five generations. Two single-cyst cultures from the Anda sample were identified as HG Type 1.2.3.5.6.7 and HG Type 1.3, and one single-cyst culture from Wuchang was identified as HG Type 2.5.7. Then 18 soybean genotypes, including 11 local cultivars originally developed for resistance to HG Type 0, were used to evaluate resistance response to the three identified

Cui Hua, Chunjie Li, Yanfeng Hu, Yanzhi Mao, Jia You, Mingze Wang, Jingsheng Chen, Zhongyan Tian, Congli Wang

Journal of Nematology , ISSUE 1, 41–50

Research paper

Energy-dense diet triggers changes in gut microbiota, reorganization of gut-brain vagal communication and increases body fat accumulation

fat accumulation. Proteobacteria isolated from cecum of HFD rats were toxic to vagal afferent neurons in culture. Our findings show that diet-induced shift in gut microbiome may disrupt vagal gut-brain communication resulting in microglia activation and increased body fat accumulation.

Alexandra C. Vaughn, Erin M. Cooper, Patricia M. DiLorenzo, Levi J. O’Loughlin, Michael E. Konkel, James H. Peters, Andras Hajnal, Tanusree Sen, Sun Hye Lee, Claire B. de La Serre, Krzysztof Czaja

Acta Neurobiologiae Experimentalis , ISSUE 1, 18–30

short-communication

Evaluation of The Pathogenic Potential of Insecticidal Serratia marcescens Strains to Humans

pathogenesis is the colonization of epithelial cells. After adhesion to the cells, the bacteria produce potential virulence factors, including extracellular toxins which are probably the most common factors of S. marcescens pathogenicity (Grimont and Grimont 2006). The pathogenicity of S. marcescens to insects makes these bacteria an interesting tool in the search of the new biological control preparation applied in plant protection. Liquid culture of S. marcescens had potential use as a biocontrol factor

EDYTA KONECKA, JOANNA MOKRACKA, SYLWIA KRZYMIŃSKA, ADAM KAZNOWSKI

Polish Journal of Microbiology , ISSUE 2, 185–191

original-paper

Resensitization of Fluconazole-Resistant Urinary Candida spp. Isolates by Amikacin through Downregulation of Efflux Pump Genes

the patients admitted to the Alexandria Main University Hospital (AMUH) in Egypt. It also focused on elucidating the role of amikacin as a resistance-modifying agent that might help in increasing the susceptibility of resistant strains to one of the most frequently used antifungal agents, fluconazole. Moreover, the underlying mechanisms of amikacin sensitization of Candida isolates to fluconazole were studied. Experimental Materials and Methods Clinical isolates and culture conditions. Twenty

EVA A. EDWARD, NELLY M. MOHAMED, AZZA S. ZAKARIA

Polish Journal of Microbiology , ISSUE 1, 73–84

Research paper

The differential effects of neuroleptic drugs and PACAP on the expression of BDNF mRNA and protein in a human glioblastoma cell line

order to confirm the observed changes in the mRNA expression of BDNF, a protein expression assay was performed. The exposure of cells only to 5 μM olanzapine for 72 h increased BDNF concentration in the culture medium by 29%. Additionally, PACAP significantly up-regulated BDNF mRNA expression in T98G cells and the obtained results correlated positively with the increased production of BDNF protein, by 22% above control. The results of the paper show that olanzapine, similarly to exogenous PACAP38

Marta Jóźwiak-Bębenista, Magdalena Jasińska-Stroschein, Edward Kowalczyk

Acta Neurobiologiae Experimentalis , ISSUE 3, 205–213

Original Paper

Isolation of Sabin-like Polioviruses from Sewage in Poland

As a complement to the active search for cases of acute flaccid paralysis, environmental sampling was conducted from January to December 2011, to test for any putative polio revertants and recombinants in sewage. A total of 165 environmental samples were obtained and analyzed for the presence of polioviruses by use of cell culture (L20B, RD and Caco-2) followed by neutralization and reverse-transcription polymerase chain reaction. Out of the 31 CPE positive samples, 26 contained one and 5 two

Agnieszka Figas, Magdalena Wieczorek, Anna Żuk-Wasek, Bogumiła Litwińska

Polish Journal of Microbiology , ISSUE 1, 89–96

Original Paper

The Emergence of Different Functionally Equivalent PAH Degrading Microbial Communities from a Single Soil in Liquid PAH Enrichment Cultures and Soil Microcosms Receiving PAHs with and without Bioaugmentation

Polycyclic aromatic hydrocarbon (PAHs) are common soil contaminants of concern due to their toxicity toward plants, animals and microorganisms. The use of indigenous or added microbes (bioaugmentation) is commonly used for bioremediation of PAHs. In this work, the biodegradation rates and changes in the bacterial community structure were evaluated. The enrichment culture was useful for unambiguously identifying members of the soil bacterial community associated with PAH degradation and yielded

FRANCINE AMARAL PIUBELI, LIGIA GIBBI DOS SANTOS, EMILIA NARANJO FERNÁNDEZ, FLÁVIO HENRIQUE DA SILVA, LUCIA REGINA DURRANT, MATTHEW JAMES GROSSMAN

Polish Journal of Microbiology , ISSUE 3, 365–375

Article

Networks and Institutionalization: A Neo-structural Approach

This paper is the text prepared for the keynote address of the EUSN 2017 conference in Mainz, Germany. A short presentation of concepts reflects in part the foundations of neo-structural sociology (NSS) and its use of social and organisational network analyses, combined with other methodologies, to better understand the roles of structure and culture in individual and collective agency. The presentation shows how NSS accounts for institutional change by focusing on the importance of combined

Emmanuel Lazega

Connections , ISSUE 1-2, 7–22

Article

EXOPOLYSACCHARIDE-PRODUCING LACTIC ACID BACTERIA – HEALTH-PROMOTING PROPERTIES AND APPLICATION IN THE DAIRY INDUSTRY

. plantarum) accounts for ca. 0.14 g/l [81], Lb. bulgaricus – 0.06–0.15 g/l [83] and Lb. fermentum – 0.75 g/l [41]. In turn, lactic streptococci (S. thermophilus) are able to produce 0.1 g/l [89]. However, the greatest production of EPS was demonstrated for Lb. rhamnosus RW-9595M strain (2.8 g/l), Lb. kefiranofaciens WT-2B strain (2.5 g/l) and Lb. plantarum BR2 strain (2.8 g/l) [58, 60, 79]. EPS production is determined by the growth stage of bacteria, composition of culture medium (type of carbon and

Anna M. Berthold-Pluta, Antoni St. Pluta, Monika Garbowska, Lidia Stasiak-Różańska

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 191–204

research-article

De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae

examined for collecting the nematodes. The collected nematodes were placed in a Petri dish filled with sterile PBS and washed several times to remove any tissue debris. Then, they were observed under a stereomicroscope and actively moving nematodes without any injury were selected for in vitro culture to obtain A. pegreffii fourth stage larvae (APL4). Each larva was treated with antibiotic-antifungal solution as described elsewhere before starting in vitro culture (Iglesias et al., 2001). Then

U-Hwa Nam, Jong-Oh Kim, Jeong-Ho Kim

Journal of Nematology , 1–16

Article

Evaluation of Pochonia chlamydosporia and Purpureocillium lilacinum for Suppression of Meloidogyne enterolobii on Tomato and Banana

Meloidogyne enterolobii is one of the most important root-knot nematode in tropical regions, due to its ability to overcome resistance mechanisms of a number of host plants. The lack of new and safe active ingredients against this nematode has restricted control alternatives for growers. Egg-parasitic fungi have been considered as potential candidates for the development of bionematicides. In tissue culture plates, Pochonia chlamydosporia (var. catenulata and chlamydosporia) and

SILAS D. SILVA, REGINA M. D. G. CARNEIRO, MARCOS FARIA, DANIELA A. SOUZA, ROSE G. MONNERAT, ROGERIO B. LOPES

Journal of Nematology , ISSUE 1, 77–85

Original Paper

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

approximately 15% of DCF and 9% of SMX after 20 days of monosubstrate culture. However, a decrease in the optical density of the MG7 strain cultures was observed, caused by an insufficient carbon source for bacterial growth and proliferation. The adsorption of pharmaceuticals onto autoclaved cells was negligible, which confirmed that the tested strain was directly involved in the removal of DCF and SMX. In the presence of phenol as the additional carbon source, the MG7 strain was able to remove

ANNA WĘGRZYN, EWA FELIS

Polish Journal of Microbiology , ISSUE 3, 321–331

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