Original Research | 18-July-2017
identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69%), followed by M. javanica (13%), M. enterolobii (2%), and M. arenaria (2%). Most of the tubers sampled (86%) were infected by a single species, and multiple species of RKN were detected in 14% of the samples. Results of both identification methods revealed the
Yao A. Kolombia,
Gerrit Karssen,
Nicole Viaene,
P. Lava Kumar,
Nancy de Sutter,
Lisa Joos,
Danny L. Coyne,
Wim Bert
Journal of Nematology, Volume 49 , ISSUE 2, 177–188
research-article | 21-October-2020
Province, Peru. In order to identify the plant-parasitic nematode species, a combination of morphological, biochemical, and molecular analyses were performed.
Figure 1:
A and B: Roots of Brassica nigra (L.) W.D.J. Koch showing galls induced by Meloidogyne incognita (Kofoid and White, 1919; Chitwood, 1949).
This population of root-knot nematode was identified to species with esterase phenotypes (n = 36 females) (Carneiro and Almeida, 2001); morphology, and morphometrics of second-stage juveniles
Jorge Airton Gómez-Chatata,
Juan José Tamo-Zegarra,
Teodocia Gloria Casa-Ruiz,
Cristiano Bellé
Journal of Nematology, Volume 52 , 1–3
research-article | 30-November-2020
nodules, characterizing the galls; C. Isoenzymatic esterase phenotype (I1 = M. incognita) of females recovered from hops roots Mi.1 and Mi.2; J3 = M. javanica (Treub, 1885) Chitwood, 1949, control. Trapezoidal labial region of male (a), a prominent labial disc in relation to the submedian lips with transverse streaks (b) and the stylet basal knobs height than wide (c); D. Perineal region of a female with high, trapezoidal dorsal arch and thick streaks, typical of M. incognita. São Paulo, Brazil
R. F. Gonsaga,
A. Souza Pollo,
D. D. Nascimento,
R. J. Ferreira,
L. T. Braz,
P. L. M. Soares
Journal of Nematology, Volume 53 , 1–4
research-article | 30-November-2020
graminicola Golden and Birchfield, 1965, root infestation symptoms on South American rush (Juncus microcephalus Kunth). Root-knot symptoms of galls of J. microcephalus from the field (A, B) and in the greenhouse (C, D).
This species was identified from esterase using esterase phenotypes (n = 20 females) (Carneiro and Almeida 2001; Carneiro et al., 2000), morphological measurement of second-stage juveniles (J2) (n = 20), females (n = 10) and males (n = 10), and perineal patterns (n = 20) and through the
Cristiano Bellé,
Paulo Sergio dos Santos,
Tiago Edu Kaspary
Journal of Nematology, Volume 53 , 1–4
research-article | 24-April-2020
identified to species with esterase phenotypes (n = 36 females) (Carneiro and Almeida, 2001); morphology and morphometrics of second-stage juveniles (J2) (n = 30) and females (n = 10), and perineal patterns (n = 15); and molecular characterization of the mitochondrial DNA region between the cytochome oxidase subunit II (COII) and 16 S rRNA genes (mtDNA) using the primers C2F3 (5´-GGTCAATGTTCAGAAATTTGTGG-3´) and 1108 (5´-TACCTTTGACCAATCACGCT-3´) (Powers and Harris, 1993) along with PCR species-specific
Ricardo Andreé Vega-Callo,
María Yaquelin Mendoza-Lima,
Nataly Ruth Mamani-Mendoza,
Leslie Sharon Lozada-Villanueva,
Juan José Tamo-Zegarra,
Teodocia Gloria Casa-Ruiz,
Cristiano Bellé
Journal of Nematology, Volume 52 , 1–3
research-article | 21-October-2020
= 20), and perineal patterns (n = 20 females), esterase phenotypes (n = 36 females), and molecular characterization of the mitochondrial DNA region between the cytochome oxidase subunit II (COII) and 16S rRNA genes (mtDNA) using the primers C2F3 and 1108 (Powers and Harris, 1993); along with PCR species-specific characterized amplified region (SCAR) sequence for confirmation, using a primer set composed of inc-K14-F and inc-K14-R (Randig et al., 2002).
The nematode population density observed in
Jorge Airton Gómez-Chatata,
Teodocia Gloria Casa-Ruiz,
Juan José Tamo-Zegarra,
Cristiano Bellé
Journal of Nematology, Volume 52 , 1–4
research-article | 30-November-2018
molecular analyses were performed.
Figure 1:
Sweet broom (Scoparia dulcis L.) roots showing galls caused by Meloidogyne javanica (Treub, 1885) Chitwood, 1949 infection.
To identify this Meloidogyne population, the following techniques were used: esterase phenotypes (n = 40 females) (Carneiro and Almeida, 2001); morphology and morphometrics of second-stage juveniles (J2) (n = 40) and females (n = 20), and perineal patterns (n = 20); and molecular characterization of the mitochondrial DNA region
Cristiano Bellé,
Rodrigo Ferraz Ramos,
Andressa Lima de Brida,
Tiago Edu Kaspary
journal of nematology, Volume 51 , 1–3
research-article | 30-November-2018
(Aydınlı et al., 2013; Carneiro et al., 2014; Bellé et al., 2016; Janssen et al., 2016; Machado et al., 2016). Currently, there are about 26 different plant species recognized as hosts for M. luci (EPPO, 2017). Because of its morphological resemblance to M. ethiopica Whitehead, 1968 and similar esterase phenotype, M. luci might have been misidentified as M. ethiopica in a number of surveys. Therefore, it is highly probable that this RKN has an even broader host range and distribution than is currently
Duarte Santos,
António Correia,
Isabel Abrantes,
Carla Maleita
Journal of Nematology, Volume 51 , 1–4
research-article | 30-November-2019
measurements in µm. a, c De Man indices; n.d. not determined.
Biochemical identification was performed by esterase phenotype analysis. Females were excised from infected roots of the turfgrass mix and transferred to sealed micro-hematocrit tubes with 5 μl of extraction buffer (20% sucrose with 1% Triton X-100) and macerated with a pestle. After centrifugation, the protein extracts were submitted to electrophoresis in polyacrylamide gels according to Pais and Abrantes (1989). Gels were stained for
M. Clara Vieira dos Santos,
M. Teresa M. Almeida,
Sofia R. Costa
Journal of Nematology, Volume 52 , 1–4
research-article | 30-November-2019
spacer (ITS) region, and the sequence characterized amplified region (SCAR) combined with morphological, morphometric and biochemical (e.g. esterase phenotyping) data provides better resolution for species identification.
We found a severe infestation of M. enterolobii in guava in Coimbatore district of Tamil Nadu, India (located at N11°1′6″ and E76°58′21″); and the identity of the species was confirmed by detailed morphology and morphometrics supplemented with biochemical and molecular
Tushar Manohar Ghule,
Victor Phani,
Vishal Singh Somvanshi,
Maya Patil,
Somnath Bhattacharyya,
Matiyar Rahaman Khan
Journal of Nematology, Volume 52 , 1–9
research-article | 09-April-2020
studies (n = 20). Additionally, individual females (n = 20) were extracted from the peach roots and identified by electrophoresis using α-esterase (Est) and malate dehydrogenase (Mdh) phenotypes (Carneiro and Almeida, 2001) and perineal patterns (Taylor and Netscher, 1974). The nematode population density in the samples was 283 eggs and J2s per gram of fresh roots. Perineal patterns of females (Fig. 2B,C) showed oval squared shapes, with moderately high to high dorsal arches, striae widely separated
W. R. Silva,
C. P. Machaca-Calsin,
C. B. Gomes
Journal of Nematology, Volume 52 , 1–3