Article
biopharmaceuticals, which are recombinant proteins with therapeutic properties. This made the mass production of safe and effective medications possible. Currently, many diseases are treated with biopharmaceuticals produced by the DNA recombination technology [7, 65].
Manufacturing biopharmaceuticals is strictly regulated by international guidelines defining the proper conduct during production process. The entire technological process, such as: selection of the expression system, cell culture, creation of cell
Agnieszka Sobolewska-Ruta,
Piotr Zaleski
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 87–100
original-paper
, Candida tropicalis, Candida parapsilosis, and C. neoformans. To further understand the physical and chemical properties of AMP-17 protein, the salt, acid-base, and thermal stabilities were also assayed. In addition, cytotoxicity and hemolytic activity of AMP-17 protein were determined, as a prelude to more extensive safety evaluation.
Experimental
Materials and Methods
Materials. AMP-17 protein was obtained from the Guoguo research team. It was produced in a prokaryotic expression system and
LONG-BING YANG,
GUO GUO,
XIN-YU ZHAO,
PEI-PEI SU,
PING FU,
JIAN PENG,
JIANG-FAN XIU,
BO-YAN LI
Polish Journal of Microbiology , ISSUE 3, 383–390