biopharmaceuticals, which are recombinant proteins with therapeutic properties. This made the mass production of safe and effective medications possible. Currently, many diseases are treated with biopharmaceuticals produced by the DNA recombination technology [7, 65].
Manufacturing biopharmaceuticals is strictly regulated by international guidelines defining the proper conduct during production process. The entire technological process, such as: selection of the expression system, cell culture, creation of cell
Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 1, 87–100
, Candida tropicalis, Candida parapsilosis, and C. neoformans. To further understand the physical and chemical properties of AMP-17 protein, the salt, acid-base, and thermal stabilities were also assayed. In addition, cytotoxicity and hemolytic activity of AMP-17 protein were determined, as a prelude to more extensive safety evaluation.
Materials and Methods
Materials. AMP-17 protein was obtained from the Guoguo research team. It was produced in a prokaryotic expression system and
Polish Journal of Microbiology , ISSUE 3, 383–390