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Short Communication

Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic Ganoderma boninense

Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological poten­tials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 104 basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from

Nisha Thopla Govender, Maziah Mahmood, Idris Abu Seman, Wong Mui-Yun

Polish Journal of Microbiology , ISSUE 3, 383–388


N-acetylcysteine (NAC) Attenuating Apoptosis and Autophagy in RAW264.7 Cells in Response to Incubation with Mycolic Acid from Bovine Mycobacterium tuberculosis Complex

and cell damage of host cells after incubation with MA. Animal experiments were performed to investigate the role of NAC in antagonizing the effects of MA in the induction of apoptosis and autophagy. Experimental Materials and Methods Animals. Male ICR (Institute of Cancer Research) mice aged six weeks were purchased from the Chinese Academy of Sciences (Shanghai, China). The Ethics Committee approved all experimental procedures for Laboratory Animals at the Ningxia University. Mice were kept


Polish Journal of Microbiology , ISSUE 2, 223–229

Original Paper

In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs

the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B1 and hydrolysed fumonisin B1 concentration were analysed by liquid chromatograghy – mass spectrometry. There was no significant difference in FB1 concentration between the experimental and the control 2 group (buffer and fumonisin B1) at 0 h incubation, 5.185 ± 0.174 μg/ml com­pared with 6.433 ± 0.076

Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, István Bors, András Bónai, Brigitta Bóta, Judit Szabó-Fodor

Polish Journal of Microbiology , ISSUE 2, 245–250

Short Communication

Effects of Selected Herbicides on Growth and Nitrogen Fixing Activity of Stenotrophomonas maltophilia (Sb16)

A study was carried out to determine the effects of paraquat, pretilachlor and 2, 4-D on growth and nitrogen fixing activity of Stenotropho­monas maltophilia (Sb16) and pH of Jensen’s N-free medium. The growth of Sb16 and pH of medium were significantly reduced with full (X) and double (2X) doses of tested herbicides, but nitrogen fixing activity was decreased by 2X doses. The nitrogenase activity had the highest value in samples treated with 1/2X of 2, 4-D on fifth incubation day

Armita Nahi, Radziah Othman, Dzolkhifli Omar, Mahdi Ebrahimi

Polish Journal of Microbiology , ISSUE 3, 377–382

Original Paper

Exopolysaccharides Produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos Affect Their Adhesion to Enterocytes

: Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos. Exopolysaccharides were isolated from bacterial cells and their monosaccharide composition was examined using liquid chromatography. The influence of exopolysaccharides on lactobacilli adhesion to enterocytes was studied after deglycosylation of the bacterial cells and incubation with the selected intestinal microbiota strains that metabolize polysaccharides – Faecalibacterium prausnitzii DSM 17677 and Blautia luti DSM 14534. Both deglycosylation


Polish Journal of Microbiology , ISSUE 3, 273–281

Research paper

The differential effects of neuroleptic drugs and PACAP on the expression of BDNF mRNA and protein in a human glioblastoma cell line

were compared to the action of PACAP38, a neuropeptide with well known BDNF-mediated neuroprotective effects. The tested neuroleptics differentially regulated the mRNA expression and protein level of BDNF depending on the concentration and incubation time. Using rtPCR technique, we demonstrate that, from the three tested neuroleptics, both haloperidol as well as olanzapine at 5 μM concentration (but not at 20 μM) increased BDNF mRNA expression with a similar efficacy after a 72 h incubation. In

Marta Jóźwiak-Bębenista, Magdalena Jasińska-Stroschein, Edward Kowalczyk

Acta Neurobiologiae Experimentalis , ISSUE 3, 205–213

Original Paper

Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1

characterization and investigation of different valuable applications. Effect of medium type, incubation period and pH were studied using the one factor at a time experiments. It was shown that the highest productivity (24 gl–1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation. Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations. The finalized optimized medium

Sahar W.M. Hassan, Hassan A.H. Ibrahim

Polish Journal of Microbiology , ISSUE 4, 449–461

Original Paper

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

, Citrobacter amolonaticus G4 and Acinetobacter lwoffii G5. This is the first study on the bacterial flora of Blatta orientalis evaluated for the biodegradation of α-endosulfan. After 10 days of incubation, the biodegradation yields obtained from P. aeruginosa G1, S. maltophilia G2, B. atrophaeus G3, C. amolonaticus G4 and A. lwoffii G5 were 88.5% , 85.5%, 64.4%, 56.7% and 80.2%, respectively. As a result, these bacterial strains may be utilized for biodegradation of endosulfan polluted soil and

Murat Ozdal, Ozlem Gur Ozdal, Omer Faruk Algur

Polish Journal of Microbiology , ISSUE 1, 63–68

Original Paper

The Usefulness of Chromogenic Media for Qualitative and Semi-Quantitative Diagnostic of Urinary Tract Infections

Agar (UTI C, Oxoid). After a 24-hour incubation period, 47 Gram-positive cocci and 62 Gram-negative rods were observed. The specificity and sensitivity of all chromogenic media was 97.3% and 93.5% respectively for qualitative diagnostic; and 81.9% and 81.3% respectively for semi-quantitative diagnostic. The mean PPV and NPV of the chromogenic media were 98.7% and 87.7% for qualitative UTI diagnostic, and 90.9% and 71.9% respectively for semi-quantitative diagnostic.


Polish Journal of Microbiology , ISSUE 2, 213–218

Original Paper

Characterization of Bacteria Isolation of Bacteria from Pinyon Rhizosphere, Producing Biosurfactants from Agro-Industrial Waste

strains belonged to Enterobacter sp., Pseudomonas aeruginosa, Bacillus pumilus and Rhizobium sp. All strains assayed were able to grow and showed halos around the colonies as evidence of biosurfactants production on Cetyl Trimethyl Ammonium Bromide agar with crude glycerol and lactoserum as substrate. In a mineral salt liquid medium enriched with both wastes, the biosurfactants were produced and collected from free cell medium after 72 h incubation. The biosurfactants produced reduced the surface

Arnoldo Wong-Villarreal, Lizbeth Reyes-López, Hipólito Corzo González, Cristina Blanco González, Gustavo Yáñez-Ocampo

Polish Journal of Microbiology , ISSUE 2, 183–189



temperature gradient between the local temperature in the measurement range of the thermopile and its internal temperature. Therefore, it is necessary to integrate temperature sensors aiming to correct the output temperature value. In this sense, the output of the thermopile corresponds to a value generated from the relationship between the internal temperature of the thermopile and the output temperature. This work proposes and evaluates a thermopile characterization model, which uses an incubation

J-S. Botero V, A. Salazar, L-J. Morantes G.

International Journal on Smart Sensing and Intelligent Systems , ISSUE 2, 637–650


Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products

. After incubation at 37°C for 10 min, the absorbance at 465 nm was measured to calculate the difference ΔA, and ultrapure water was used as a control. An enzyme activity unit was defined as the amount of enzyme required to oxidize 1 nmol of guaiacol per minute per liter of the culture medium. The guaiacol extinction coefficient ε2 was equal to 12,100 l · mol–1 · cm–1, and the calculation formula was as follows: Lip (nmol·min−1·1−1)=ΔA÷(ε1×d)×VA÷VS÷TMnp (nmol·min−1·1−1)=ΔA÷(ε2×d)×VA÷VS÷T d – cuvette


Polish Journal of Microbiology , ISSUE 3, 339–347


Silver nanoparticles as a potential nematicide against Meloidogyne graminicola

46.7 68.3 0.5 60 83.3 80 1.0 73.3 96.7 93.3 2.0 100 100 100 3.0 100 100 100 Notes: aControl (no AgNP, water alone). Means are averages of three replications. Data were subjected to 9 × 3 (AgNP concentration × incubation time) factorial analysis of variance (ANOVA), critical difference (CD) for incubation time is 0.027, CD for AgNP concentrations is 0.468, and that for time ×  concentrations is 0.81. AgNP effects on rice grown in a soilless system The AgNP treatments at 1, 2, and 3 µg

Richa Baronia, Puneet Kumar, S. P. Singh, R. K. Walia

Journal of Nematology , 1–9

Original Paper

Dipicolinic Acid Release and the Germination of Alicyclobacillus acidoterrestris Spores under Nutrient Germinants

. Optical density (OD660) was used to estimate the number of germinated spores. The amount of DPA released was determined using HPLC. The results indicate that the degree of germination of A. acidoterrestris spores depended on the strain and time of incubation and the nutritious compounds used. The data obtained show that the amount of DPA released correlated to the number of A. acidoterrestris spores germinated.

Izabela Porębska, Barbara Sokołowska, Łukasz Woźniak

Polish Journal of Microbiology , ISSUE 1, 67–74

Original Paper

Evaluation of Neisseria meningitidis Carriage with the Analysis of Serogroups, Genogroups and Clonal Complexes among Polish Soldiers

types and clonal complexes of the isolates detected among Polish soldiers. The study was conducted during winter seasons of 2015 and 2016 and involved 883 professional soldiers from the Armoured Brigade in Świętoszów, Poland. The material for testing were nasopharyngeal swabs obtained from study participants. The samples were tested using standard microbiological methods (culture, incubation, microscopy, biochemical and automated identification). N. meningitidis isolates were subjected to slide


Polish Journal of Microbiology , ISSUE 4, 493–500



more efficient in samples with mycelium immobilized on a polypropylene foam, what probably was associated with increased enzyme activity of the strains, as well as enhancement of the contact of the dye with the mycelium. Strain L3 respectively removed 100% (mycelium immobilized) of the dye after 24h and 95.8% (mycelium suspended) of the dye after 96h. For complete removal of the dye the immobilized biomass of strain L3 needs 24 hours of incubation, and L1 48h. Strain L1 completely removed the color


Architecture, Civil Engineering, Environment , ISSUE 1, 137–145


Comparison of Rapid and Routine Methods of Identification and Antibiotic Susceptibility Testing of Microorganisms from Blood Culture Bottles

patient is given the opportunity to start early antibiotic treatment. Continuous monitoring of automated blood culture systems allows a positive blood culture to be detected within 6 to 24 hours of incubation (Morrell et al. 2005; Behera et al. 2010; Ferreira et al. 2011; Kirn et al. 2014; Nataraj et al. 2016). In recent years, advances in the methods involving polymerase chain reaction and DNA sequencing have changed the nature of clinical microbiology laboratories. However, the lack of diagnostic


Polish Journal of Microbiology , ISSUE 2, 165–176

Original Paper

The Emergence of Different Functionally Equivalent PAH Degrading Microbial Communities from a Single Soil in Liquid PAH Enrichment Cultures and Soil Microcosms Receiving PAHs with and without Bioaugmentation

a low diversity community. No significant difference in the rate of PAH degradation was observed between the microcosm receiving only PAHs or PAHs and bioaugmentation. Moreover, identical matches to the bioaugmentation inoculum were only observed at the initial stages of PAH degradation on day 8. After 22 days of incubation, the substantial degradation of all PAHs had occurred in both microcosms and the PAH contaminated soil had statistically significant increases in Alphaproteobacteria. There


Polish Journal of Microbiology , ISSUE 3, 365–375

Original Paper

Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis

spectrum of Staphylococcus spp. clinical and animal isolates. Growth inhibition was observed only in the case of 13 (including nine S. aureus, three S. xylosus and one S. epidermidis strains) out of 206 strains tested. Important advantage of the produced agent was its high thermal stability. Fifteen minutes of incubation at 90°C did not affect its antimicrobial potential. The highest efficiency of production of the agent was demonstrated in TSB medium after 24 hours at 37°C. The researches revealed


Polish Journal of Microbiology , ISSUE 2, –


Reproduction and life history traits of a resistance breaking Globodera pallida population

James M. Mwangi, Björn Niere, Maria R. Finckh, Stefan Krüssel, Sebastian Kiewnick

Journal Of Nematology , 1–13


Activity of Steinernema colombiense in plant-based oils

with ventilation, and stored in laboratory conditions at least for a week before being used (Chiriboga et al., 2017). Combination of entomopathogenic nematodes with plant-based oils at different temperatures and times We assessed for the survival and virulence of S. colombiense combined with coconut oil, olive oil, or water (control) at five temperatures (4, 8, 14, 20, and 24°C) and five incubation times (1, 3, 7, 14, and 21 days). We used 24-wells plates (Falcon Multiwell, 24-well Polystyrene

Gabriela Castruita-Esparza, Francisco Ángel Bueno-Pallero, Rubén Blanco-Pérez, Lídia Dionísio, Teodulfo Aquino-Bolaños, Raquel Campos-Herrera

Journal of Nematology , 1–12


In Vitro and In Vivo Activity of Zabofloxacin and Other Fluoroquinolones Against MRSA Isolates from A University Hospital in Egypt

(1999). Two isolates, one resistant and one susceptible to the four tested fluoroquinolones, named S13 and S15 respectively, were selected for this analysis. The bacterial cultures in Muller-Hinton broth (Oxoid, UK) were diluted to reach an approximate count of 105 to 106 CFU/ml and preincu bated for 2 h. Then, ZAB, MOX, LEV and CIP were added to the cultures at concentrations of 0.5, 1, 2, and 4 MIC. The bacterial count was determined after 0, 3, 6 and 24 h of incubation with the antibiotics tested


Polish Journal of Microbiology , ISSUE 1, 59–69

Original Paper

Use of standard laboratory methods to obviate routine dithiothreitol treatment of blood samples with daratumumab interference

solid phase led to tube testing with either low-ionicstrength saline, polyethylene glycol, or both. If incubation failed to eliminate the reactivity, the sample was sent to a reference laboratory for DTT treatment and phenotyping. Of the 33 samples tested, 23 (69.7%) samples had reactivity in solid-phase testing. In 8 of the 10 samples that did not react in solid-phase, testing was conducted more than four half-lives after the last dose of daratumumab. Of the 23 that had reactivity in solid-phase

Nicholas J. Lintel, Debra K. Brown, Diane T. Schafer, Farai M. Tsimba-Chitsva, Scott A. Koepsell, Sara M. Shunkwiler

Immunohematology , ISSUE 1, 22–26

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