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Article | 30-November-2019

Inhibition of blood group antibodies by soluble substances

Background When performing pretransfusion testing, serologic results may indicate the presence of one or more alloantibodies. There are many methods that can be used to identify and separate specificities.1 One such method is based on the principle of inhibition. The ability to specifically inhibit one antibody may help identify that antibody and allow other antibody specificities to also be identified. Inhibition can aid in the identification of an antibody to an antigen that shows variable

K.M. Byrne, C.M.C. Mercado, T.N. Nnabue, T.D. Paige, W.A. Flegel

Immunohematology, Volume 35 , ISSUE 1, 19–22

Research paper | 01-December-2011


This paper presents a biologically-inspired signal processor, which allows selective amplification of signals, and signal channeling mechanism to increase the contrast and sharpness of the perceived signals. The processor draws influence from lateral inhibition and integrative function mechanisms of the human nervous system. The uniqueness of the proposed design is that it combines two intelligent signal transmission mechanisms of the human nervous system with desirable capabilities: 1) wide

Kiran George, Chien-In Henry Chen

International Journal on Smart Sensing and Intelligent Systems, Volume 4 , ISSUE 4, 547–567

research-article | 07-July-2020

Temporal specificity of latent inhibition in rats with daily water restriction prior to taste conditioning

INTRODUCTION The formation of latent inhibition (LI), that is a reduced conditioned response due to pre-exposures to the future conditioned stimulus (CS) before conditioning, is sensitive to certain contextual changes between different stages in the procedure (Hall and Channell, 1986; Escobar et al., 2002). Particularly, LI of taste aversion learning (CTA) can be modulated by contextual changes to the external or physical environment (De la Casa and Lubow, 1995; 2001; Lukoyanov et al., 2002

Andrés Molero-Chamizo, Guadalupe Nathzidy Rivera-Urbina

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 2, 99–107

Article | 20-April-2020

An unusual anti-H lectin inhibited by milk from individuals with the Bombay phenotype

There are several lectins with anti-H specificity but few of them serve as useful reagents.An anti-H lectin,extracted from the seeds of the plant Momordica dioica Roxb. ex willd., was tested for its hemagglutination and inhibition properties, using standard serologic methods and panel RBCs, serum, saliva, milk, and oligosaccharides purified from milk. The extract displayed strongest agglutination with group O RBCs and was weakest with group A1B RBCs in a spectrum of O>A2>B>A2B>A1>

Sanmukh R. Joshi, K. Vasantha, Janine S. Robb

Immunohematology, Volume 21 , ISSUE 1, 1–4

Article | 29-December-2020

Evaluation of monoclonal anti-A reagents by salivary inhibition studies

Ten commercially available monoclonal and polyclonal anti-A reagents were evaluated for use in the test for ABH secretor status after finding unexpected inhibition with a monoclonal anti-A (reagent Z). Two of the available monoclonal reagents were from different batches from the same clone as reagent Z. Those two reagents, as well as reagent Z, appeared to he partially neutralized by salivary substances from group A nonsecretor individuals. This finding suggests that when a saliva inhihition

Stephen M. Henry, Graeme Benny, Graeme Woodfield

Immunohematology, Volume 4 , ISSUE 4, 75–78

research-article | 06-April-2020

EEG correlates of cognitive load in a multiple choice reaction task

inhibit old or no more relevant information is considered to be critical for efficient WM functioning (Zacks and Hasher, 1994). Poor inhibition leads to intruding of irrelevant information into WM and reduces its capacity (Vogel and Machizawa, 2004; Borella, 2008). The inhibition is also critical for a situation in which multiple stimulus-response (S-R) associations (S-R mappings) or task sets should be maintained in WM and compete with each other for an access to a motor response. This kind of

Sergei A. Schapkin, Jonas Raggatz, Markus Hillmert, Irina Böckelmann

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 1, 76–89

short-communication | 19-March-2021

Growth Inhibition of Phytopathogenic Fungi and Oomycetes by Basidiomycete Irpex lacteus and Identification of its Antimicrobial Extracellular Metabolites

wild and human life (Loiseleur 2017). Previous studies have documented efficient in vitro antagonism of basidiomycete species against phytopathogenic microorganisms, through the use of more than one mechanism (White and Traquair 2006; Gholami et al. 2019). A wide variety of extracts and metabolites obtained from basidiomycete fungi have shown antimicrobial activity, sufficient for growth inhibition of pathogenic microorganisms of medical and agricultural relevance (Shen et al. 2017). Due to the


Polish Journal of Microbiology, Volume 70 , ISSUE 1, 131–136

Research Article | 23-May-2019


ability for human-to-human transmission is higher for HCoV-SARS than for HCoV-MERS (8 generations vs. 4, respectively). Moreover, there are differences in genome structure and pathogenic mechanisms: different receptor, cell entry mechanism, different way of host response modulation (e.g. inhibition of IFNβ cascade), etc. Probably, these differences influence the overall manifestation of the disease in humans. Infection caused by HCoV-MERS might manifest itself as ARDS, a mild-mannered and

Katarzyna W. Pancer

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 1, 22–32

Research Article | 24-May-2019


phenomenon. A contact-dependent growth inhibition (CDI), primarily found in Escherichia coli strains, utilizes CdiB/CdiA protein of type Vb secretion system, described also as two-partner secretion (TPS) system, to inhibit growth of non-kin strains, where cell contact is required. Presence of an intracellular small immunity protein (CdiI) protects E. coli cells from autoinhibition. Other bacterial competition system, involved mainly in the nodulation process of Rhizobium leguminosarum bv. Trifolii strain

Dawid Gmiter, Grzegorz Czerwonka, Wiesław Kaca

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 4, 360–373

Report | 16-March-2020

Development and validation of a fluorescent microsphere immunoassay for anti-IgA

at dilutions up to 32-fold greater than the dilution used to test patients’ samples. Inhibition studies with purified IgA and IgA-deficient serum showed no inhibition with IgA-deficient serum and complete inhibition with soluble IgA. Clinical tests performed in more than 90 assays had a CV of 13.6 percent for measurements of an internal positive control. The fluorescent immunoassay method is rapid, reproducible, and sensitive to low concentrations of IgG anti-IgA.

Kandelaria M. Rumilla, Jeffrey L. Winters, Jessica M. Peterman, Eric A. Jedynak, Henry A. Homburger

Immunohematology, Volume 25 , ISSUE 1, 24–28

research-article | 23-April-2019

Nematicidal Activity of Fosthiazate Against Soybean Cyst Nematode Heterodera glycines

. glycines; hatching-inhibition effectiveness on free eggs in vitro; effect of fosthiazate on reproduction of SCN using a pot test. The results provide important information on fosthiazate for further studies controlling H. glycines and field precision applications. Materials and methods Chemicals Fosthiazate (85.1%) was obtained from the Zhengbang Bio-chemical Co., Ltd. (Nanchang, China). In bioassay, five fosthiazate concentrations (2.18, 3.44, 5.45, 8.61, and 13.62 mg/l) were used in experiments

Hai Yan Wu, Man Luo, Lu Yuan Zhang, Xun Bo Zhou

Journal of Nematology, Volume 51 , 1–9

Article | 03-November-2020

A comprehensive IgA service provided by a blood transfusion center

IgA is best known in transfusion practice for its deficiency when antiIgA antibodies cause severe anaphylactic reactions. Following the realization that IgA deficient products were needed on demand, blood donors were routinely screened, initially by latex agglutination inhibition and subsequently by hemagglutination inhibition using an Olympus PK-7200™ blood grouping machine. IgA deficiency (<.0016 g/L) was found in 357 (with anti-IgA in 28%) of 301,310 donors, an incidence of 1 in 844

R. Munks, J.R. Booth, R.J. Sokol

Immunohematology, Volume 14 , ISSUE 4, 155–160

Short Communication | 26-August-2016

Search of the Antimicrobial Potential of Benzimidazole Derivatives

activity of the synthesized compounds. From the presented set of 22 compounds, only 8, 16, 18 and 19 showed moderate to good inhibition against bacterial strains. Against Candida strains only compound 19 with three hydroxyl substituted benzene moiety presented high inhibition at nystatin level or lower.

Monika Janeczko, Zygmunt Kazimierczuk, Andrzej Orzeszko, Andrzej Niewiadomy, Ewa Król, Ryszard Szyszka, Maciej Masłyk

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 359–364

Original Paper | 04-September-2018

RNA Quality Control Using External Standard RNA

mRNA, inhibition to DNA polymerase, and degradation of mRNA for determining the RNA quality using standard RNA. It would be possible to know yield of mRNA and inhibition of the enzyme reaction by adding standard RNA before RNA extraction and looking at standard RNA loss. Degradation was evaluated by comparing the differences in the 3’ and 5’ regions of the RNA. In our study, it was demonstrated that in the crude extract of Saccharomyces cerevisiae, degradation was comparatively higher at the 3’ end


Polish Journal of Microbiology, Volume 67 , ISSUE 3, 347–353

original-paper | 08-September-2020

Marine Sediment Recovered Salinispora sp. Inhibits the Growth of Emerging Bacterial Pathogens and other Multi-Drug-Resistant Bacteria

isolated from distinct locations may produce new molecules, though an accurate identification of the isolate is compulsory (Goodfellow and Fiedler 2010). It is essential to evaluate bacterial growth inhibition by new marine Salinispora strains to find novel antibiotics for fighting the organisms of clinical importance and multi-drug-resistant bacteria. In the present work, obligate marine actinobacteria isolated from Punta Arena de la Ventana (PAV), the Gulf of California (GC), Mexico, were identified


Polish Journal of Microbiology, Volume 69 , ISSUE 3, 321–330

Research Article | 17-October-2018

Nematicidal Weeds, Solanum nigrum and Datura stramonium

We investigated Solanum nigrum (seeds) and Datura stramonium (shoots) against root-knot nematodes in terms of J2 paralysis and egg hatch inhibition (methanol extract), as well as inhibition of nematode development in host roots (soil amending with either S. nigrum seeds’ or D. stramonium shoots’ meal). Datura stramonium was found equally effective at inhibiting motility of Meloidogyne incognita and Meloidogyne javanica (both EC50 = 427 μg mL−1 at 3 day), but inhibition occurred more quickly for

Chrisostomos Oplos, Kodjo Eloh, Urania-Menkissoglu Spiroudi, Caboni Pierluigi, Nikoletta Ntalli

Journal of Nematology, Volume 50 , ISSUE 3, 317–328

Short Communication | 04-December-2017

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Moreno Bondi, Anna Mazzini, Simona De Niederhäusern, Ramona Iseppi, Patrizia Messi

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 533–536

Original Paper | 26-August-2016

Distribution and Identification of Endophytic Streptomyces Species from Schima wallichii as Potential Biocontrol Agents against Fungal Plant Pathogens

Streptomyces sp. associated with an ethanobotanical plant Schima wallichi. A total of 22 actinobacterial strains were isolated from different organs of selected medicinal plants and screened for their biocontrol ability against seven fungal phytopathogens. Seven isolates showed significant inhibition activity against most of the selected pathogens. Their identification based on 16S rRNA gene sequence analysis, strongly indicated that all strains belonged to the genus Streptomyces. An endophytic strain

Ajit Kumar Passari, Vineet Kumar Mishra, Vijai Kumar Gupta, Ratul Saikia, Bhim Pratap Singh

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 319–329

Original Paper | 30-March-2017

Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

periodontitis patients. In vitro laboratory susceptibility was carried out using the modified agar diffusion method by measuring the diameters of inhibition zones on agar plates coated with selected microbial strains. Both calcium chloride (CaCl2) activated and non activated samples were tested. Both activated PRP and PPP, of both patients and controls, effectively inhibited the growth of A. actinomycetemcomitans, P. gingivalis and C. albicans. However, a statistically significant difference in favor of PRP

Reham L. Aggour, Lina Gamil

Polish Journal of Microbiology, Volume 66 , ISSUE 1, 31–37

original-paper | 03-September-2019

Purification, Characterization and Inhibition of Alanine Racemase from a Pathogenic Strain of Streptococcus iniae

explored the potentiality of the enzyme as an attractive antimicrobial target against S. iniae. We determined the 50% inhibition concentrations (IC50) of two alanine racemase inhibitors (homogentisic acid and hydroquinone) and their antimicrobial susceptibility against six opportunistic pathogens including S. iniae, in quest of providing the possible solutions against antibiotics resistance and bacterial infections. Experimental Materials and Methods Strains, plasmids, and growth conditions. The


Polish Journal of Microbiology, Volume 68 , ISSUE 3, 331–341

Article | 17-November-2020

Trimeresurus venom inhibition of anti-HPA-1a and anti-HPA-1b antibody binding to human platelets

Steve J. Wlodar, Darryl L. Stone, Lyle T. Sinor

Immunohematology, Volume 11 , ISSUE 4, 129–132

Article | 14-October-2020

False reactivity in GTI Pak Plus® ELISA kits due to the presence of anti-mouse antibody in patients' samples

210 patients were evaluated from January 1995 to April 2002; 79 (38%) were found to be positive for HLA- and/or platelet-specific antibodies. Thirty (38%) of these positive samples, as well as ten negative samples that served as controls, underwent HAMA neutralization/inhibition procedures before being retested by ELISA. One (10%) of the control samples was reactive after treatment. When the samples that were positive in routine testing were treated to neutralize/inhibit HAMAs, reactivity was

Miriam Fogg Leach, James P. AuBuchon

Immunohematology, Volume 19 , ISSUE 4, 112–116

review-article | 30-November-2019

Updating the picture of layer 2/3 VIP-expressing interneuron function in the mouse cerebral cortex

al., 1978; Lamour et al., 1983; Ferron et al., 1985). In the hippocampus, VIP action on pyramidal cells is also diverse (Haas and Gähwiler, 1992; Cunha-Reis et al., 2004). VIP signaling was shown to potentially influence the operation of local circuits in the hippocampus, mainly by supporting release of inhibition, exerted by other interneurons, on the pyramidal cells (Cunha-Reis et al., 2004). At least two subtypes of VIP cells selectively innervate other inhibitory interneurons in the

Anna Posłuszny

Acta Neurobiologiae Experimentalis, Volume 79 , ISSUE 4, 328–337

Original Paper | 28-December-2016

Antistaphylococcal Activity of Selected Thiourea Derivatives

Five of thiourea derivatives were prepared using as a starting compound 3-(trifluoromethyl)aniline, 4-chloro-3-nitroaniline, 1,3-thiazol-2-amine, 2H-1,2,3-triazol-4-amine and commercial isothiocyanates. All compounds were evaluated in vitro for antimicrobial activity. Derivatives 2 and 3 showed the highest inhibition against Gram-positive cocci (standard and hospital strains). The observed MIC values were in the range of 0.5–8 μg/ml. The products effectively inhibited the formation of

Joanna Stefańska, Karolina Stępień, Anna Bielenica, Małgorzata Wrzosek, Marta Struga

Polish Journal of Microbiology, Volume 65 , ISSUE 4, 451–460

research-paper | 14-June-2019

Selective neuronal death following exposure to methylenedioxypyrovalerone is accompanied by an inhibition of NMDA receptor NR2B subunit expression

inhibition of NMDAR (via D2R dependent reduction of CaMKII activity and NMDA phosphorylation) and the suppression of the indirect striatal pathway, leading to hypermotility (see a recent study by Giannotti et al., 2017). Selective vulnerability of striatal neurons to neurotoxic damage due to methamphetamine exposure (ultimately evoked by excess dopamine) has been reported previously (Zhu et al., 2006; Tulloch et al., 2011). Activation of the extrasynaptic (perisynaptic) NMDARs, predominantly consisting

László István Gerecsei, Tamás Balázsa, Diego Echevarría, Ágota Ádám, Gergely Zachar, András Csillag

Acta Neurobiologiae Experimentalis, Volume 79 , ISSUE 1, 92–100

Hypothesis | 30-August-2017

Do minocycline and other suppressors of microglia reactivity have a future in prevention or treatment of epilepsy?

potential usefulness of microglia regulators and anti-inflammatory agents in designing antiepileptic/antiepileptogenic drugs, with the primary mechanism of action based on the inhibition of neuroinflammation. Methods. We reviewed PubMed and MEDLINE databases to select publications in the topic: epilepsy, neuroinflammation, microglia and microglia regulators with antiepileptic properties. We searched the databases up to April 2017 with no date restrictions. Review and Discussion. In the present paper, we

Władysław Lasoń, Joanna Ślusarczyk, Magdalena Regulska, Monika Leśkiewicz, Agnieszka Basta-Kaim

Journal of Epileptology, Volume 25 , ISSUE 1-2, 9–20

Short Communication | 26-August-2016

Effects of Selected Herbicides on Growth and Nitrogen Fixing Activity of Stenotrophomonas maltophilia (Sb16)

, but 2X of 2, 4-D had the most adverse effect. An inhibition in the growth and nitrogenase activity was recovered on the last days of incubation.

Armita Nahi, Radziah Othman, Dzolkhifli Omar, Mahdi Ebrahimi

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 377–382

short-communication | 05-August-2020

In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus

cm distance from the edge. The Petri plates inoculated with M. cannonballus alone were used as control. Four Petri plates per treatment were used. The Petri plates were incubated at 25 ± 2°C, and the radial growth of M. cannonballus was measured after 5–7 days of incubation. The mycelial growth inhibition was calculated using the following formula: % inhibition=[1−(T/C)]×100 where C – radial growth of M. cannonballus in the control plate and T – radial growth of M. cannonballus in the dual


Polish Journal of Microbiology, Volume 69 , ISSUE 3, 379–383

Article | 27-December-2020

An update on Rodgers and Chido, the antigenic determinants of human C4

Rodgers (Rg) and Chido (Ch) blood groups are antigenic determinants of the fourth component of human complement (C4). Nine determinants have been defined by means of hemagglutination-inhibition (HAI) with polyspecific human antiserums. The association of C4A isotypes with Rg and of C4B isotypes with Ch is strong hut not complete. Derived amino acid sequences from the C4d region of selected C4 allotypes of known antigenic expression have provided support for the previously reported complex

Carolyn M. Giles

Immunohematology, Volume 5 , ISSUE 1, 1–6

Article | 29-December-2020

Immunoglobulin A (IgA) levels in blood products and plasma derivatives

Both anaphylactic and anaphylactoid reactions to transfused blood products and plasma derivatives have occurred in IgA-deficient patients with antibodies to IgA. This study reports the concentration of IgA in various blood products to assist in the development of treatment protocols for IgA-deficient patients. Five types of blood products and seven types of plasma derivatives were tested for the presence of IgA using a semiquantitative hemagglutination inhibition method. The concentration of

Susan M. Fox, Linda M. Stavely-Haiber

Immunohematology, Volume 4 , ISSUE 1, 5–9

Research paper | 25-July-2017

Inhibition of neuronal and inducible nitric oxide synthase does not affect the analgesic effects of NMDA antagonists in visceral inflammatory pain

Dragana Srebro, Sonja Vučković, Milica Prostran

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 2, 110–116

Original Paper | 26-August-2016

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source

Aurea I. Morales-Estrada, Ahidé López-Merino, Nestor Gutierrez-Mendez, Enrico A. Ruiz, Araceli Contreras-Rodríguez

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 279–285

Article | 14-October-2020

Serologic aspects of treating immune thrombocytopenic purpura using intravenous Rh immune globulin

In patients with immune thrombocytopenic purpura (ITP), IgG autoantibody-coated platelets are phagocytized by mononuclear macrophages, primarily in the spleen. Intravenous Rh immune globulin (IV RhIG) has been used since 1983 to treat D+, nonsplenectomized patients with ITP. The beneficial therapeutic effect of IV RhIG is attributed to competitive inhibition of phagocytosis of IgG-coated platelets by IgG anti-D-coated D+ red blood cells (reticuloendothelial or Fc receptor blockade). Following

Can M. Savasman, S. Gerald Sandler

Immunohematology, Volume 17 , ISSUE 4, 106–110

Article | 16-February-2021

An update on the Cartwright (Yt) blood group system

Cartwright antibodies, along with several others, could be missed in the DTT test environment.9 Inhibition studies using srYt were instrumental in further characterizing the three new Yt antigens discovered in recent years—YTEG, YTLI, and YTOT. Nomenclature The first two antigens, Yta and Ytb, were named after the patient, Mrs. Cartwright, in whom the antibody was first identified; the third antigen, YTEG, was named after the proband; and the fourth and fifth, YTLI and YTOT, were named after the

M.R. George

Immunohematology, Volume 35 , ISSUE 4, 154–155

original-paper | 05-December-2019

Inhibition of Drug Resistance of Staphylococcus aureus by Efflux Pump Inhibitor and Autolysis Inducer to Strengthen the Antibacterial Activity of β-lactam Drugs

). Several in vitro studies have shown that TZ significantly increases the susceptibility of MRSA to β-lactam antibiotics (Poulsen et al. 2013). It has been shown that cytoderm synthesis and autolysis are linked since the inhibition of the former activates the latter. Thus, the destruction of the cytoderm is an important step in the bactericidal process of penicillin and other antibiotics (Zore et al. 2011). Miconazole (MCZ) is an antifungal drug that is considered as an autolysis inducer, which causes a


Polish Journal of Microbiology, Volume 68 , ISSUE 4, 477–491

Short Communication | 25-July-2017

Somatic and dendritic perforated-patch recordings reveal β-adrenergic receptor-induced depolarization in medial prefrontal cortex pyramidal neurons

cyclase inhibitor SQ 22536. This suggested that adenylate cyclase was involved in mediating the effect of the β-adrenergic receptor agonist. The isoproterenol-induced depolarization persisted after inhibition of protein kinase A with H-89. The effect of β-adrenergic receptor activation on the membrane potential was dependent on Ih channels because it was abolished in the presence of the Ih channel inhibitor ZD 7288. Dendritic recordings were also performed. In the dendritic segments between 100 μm and

Bartłomiej Szulczyk

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 2, 158–164

original-paper | 03-September-2019

Chemical Composition of Aspergillus creber Extract and Evaluation of its Antimicrobial and Antioxidant Activities

disks (without solvent) were used as negative controls. All experiments were carried out in triplicate. After incubation, antimicrobial activity was determined by measuring the diameter of inhibition zones around the discs. Determination of minimum inhibitory concentration (MIC). The minimum inhibitory concentration (MIC) of EAF was carried out against the sensitive test microorganisms by using broth microdilution method following the recommendations of Clinical and Laboratory Standards Institute


Polish Journal of Microbiology, Volume 68 , ISSUE 3, 309–316

original-paper | 04-June-2020

Lactobacillus fermentum JX306 Restrain D-galactose-induced Oxidative Stress of Mice through its Antioxidant Activity

supernatant was measured at 510 nm. The specific details of the measurement of hydroxyl radical scavenging ability of LAB cells were as follows: Scavenging activity (%)=[(Asample−Acontrol)/(Ablank−Acontrol)]×100 Lipid peroxidation inhibition rate. The lipid peroxidation inhibition rate of the primary screening LAB strains was determined based on a Kullisaar’s test method (Kullisaar et al. 2003) with some modifications. Briefly, 1.0 ml of the LAB suspension was added to a solution containing 0.5 ml of


Polish Journal of Microbiology, Volume 69 , ISSUE 2, 205–215

research-article | 07-July-2020

Hypothermia in the course of acute traumatic spinal cord injury

phases of nerve tissue damage, but the greatest beneficial effects have been observed during the first stages of injury (Table I). In acute SCI (within 48 h of injury), hypothermia reduces blood flow, oxygen consumption and total metabolic activity in the tissue, oxidative stress and the formation of free radicals are reduced and inhibition of lactate formation prevents the development of acidosis. These factors lead to decreased edema formation. Concomitantly, with the reduction in blood supply to

Jozef Kafka, Nadezda Lukacova, Igor Sulla, Marcela Maloveska, Zuzana Vikartovska, Dasa Cizkova

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 2, 172–178

Original Paper | 10-December-2018

Emodin Reduces the Activity of (1,3)-β-D-glucan Synthase from Candida albicans and Does Not Interact with Caspofungin

Abstract Candidiasis is the most common opportunistic yeast infection, with Candida albicans as a paramount causative species. (1,3)-β-D-glucan is one of the three main targets of clinically available antifungal agents used to treat Candida infections. It is one of the most abundant fungal cell wall components. Echinocandins represent the newest class of antifungals affecting cell wall biosynthesis through non-competitive inhibition of (1,3)-β-D-glucan synthase. Therefore, treatment with


Polish Journal of Microbiology, Volume 67 , ISSUE 4, 463–470

original-paper | 28-June-2019

Prevalence and Antimicrobial Properties of Lactic Acid Bacteria in Nigerian Women During the Menstrual Cycle

), ciprofloxacin (30 µg), ofloxacin (30 µg), and gentamycin (30 µg) were placed on the surface of the solidified agar with the aid of sterile forceps and incubated aerobically at 37°C for 24 h. The susceptibility of the test organisms to the antibiotics used was documented by measuring the diameter of the clear zones of inhibition in millimeter (mm) around the antibiotics disks, and the results were interpreted according to the guidelines of European Committee on Antimicrobial Susceptibility Testing (2015


Polish Journal of Microbiology, Volume 68 , ISSUE 2, 203–209

original-paper | 05-December-2019

New Look on Antifungal Activity of Silver Nanoparticles (AgNPs)

concerning growth inhibition of other bacteria (Enterococcus spp.) and yeast (Candida albicans) by nanosilver is also available (Kim et al. 2007; Roe et al. 2008; Lara et al. 2015). However, the studies of AgNPs interaction with cell structures of bacteria and yeasts are scarce. Only in few reports the location of AgNPs inside C. albicans cells was demonstrated under transmission electron microscopy (TEM) or scanning electron microscopy (SEM) (Kim et al. 2009; Radhakrishnan et al. 2018). In opposite to


Polish Journal of Microbiology, Volume 68 , ISSUE 4, 515–525

Research Article


bring about changes in the structure of their cell membranes, resulting in their growth inhibition and cell lysis. However, there is still an untapped bioremediation potential in microorganisms, which are able to participate in the catabolism of cresols, both under aerobic and anaerobic conditions. The typical strategies of the aerobic degradation of cresols include the use of monooxygenase and dioxygenase enzymes. Thanks to these enzymes, atoms of molecular oxygen initiate fission of the aromatic

Magdalena Zaborowska

Postępy Mikrobiologii - Advancements of Microbiology, Volume 56 , ISSUE 1, 7–17

original-paper | 14-August-2020

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

McFarland scale) and then swabbed onto Mueller-Hinton agar. Holes with 4 mm diameter were created using sterile cork borer (4 mm) and filled with 20 µl of B14 cell-free supernatant of the optimized cultures. Plates were incubated at 37°C for 24 hr. The zone of inhibition was determined by measuring a clear zone around the holes. Tetracycline (30 µg/ml) was used as a positive control. Results Effect of carbon sources on growth and biosurfactant production by strain B14. In order to determine a


Polish Journal of Microbiology, Volume 69 , ISSUE 3, 273–282

Article | 21-July-2017

Assessment of DAPG-producing Pseudomonas fluorescens for Management of Meloidogyne incognita and Fusarium oxysporum on Watermelon

Fon resulted in shorter vine lengths than treatment with either P. fluorescens isolate plus RKN. Root weights, galling indices, eggs per gram root, and secondstage juvenile (J2) numbers in soil were similar among all RKN-inoculated treatments, and fruit production was not affected by treatment. Plant death was high in all treatments. These studies demonstrated that the tested P. fluorescens isolates resulted in some inhibition of vine growth in the field, and were not effective for enhancing plant


Journal of Nematology, Volume 48 , ISSUE 1, 43–53

research-article | 06-April-2020

Hindbrain metabolic deficiency regulates ventromedial hypothalamic nucleus glycogen metabolism and glucose-regulatory signaling

glycolytic end-product L-lactate is monitored in the hindbrain as a critical metabolic variable. The astrocyte-neuron lactate shuttle hypothesis posits that astrocyte lactate trafficking sustains mitochondrial energy production in neurons (Broer et al., 1997; Pellerin et al., 1998; Magistretti et al., 1993). Pharmacological induction of lactoprivation within the DVC, involving inhibition of local monocarboxylate transporter function, is a potent stimulus for hyperglycemia (Patil and Briski, 2005). During

Karen P. Briski, Santosh K. Mandal

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 1, 57–65

research-article | 15-October-2020

Diazepam and electrical stimulation of paleocerebellar cortex inhibits seizures in pentylenetetrazol-kindled rats

-Herrera et al., 2004, 2010; Besio et al., 2013; Gersner et al., 2016). These data point to the potential for the development of new and better therapies for drug-resistant seizures (Asgari et al., 2014, 2016). This study presents the first analysis of BDZ-GABAA-dependent mechanisms of seizure inhibition in response to paleocerebellar ES. To test this, we used the PTZ-induced kindling rat model since PTZ is a selective antagonist of the GABAA receptor-chloride ionophore complex (Erkec and Arihan, 2015

Leonid S. Godlevsky, Oleksii O. Shandra, Mykhailo P. Pervak, Alexey A. Shandra

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 3, 322–330

Article | 10-November-2020

Do monocyte ADCC assays accurately predict the severity of hemolytic disease of the newborn caused by antibodies to high-frequency antigens?

related to fetal antigen expression and tissue distribution. The anti-Inb was further investigated using mono­cyte binding and inhibition studies. The absence of HDN in the pres­ence of potent anti-U remains unexplained. In general, antibodies with low ADCC activity do not cause HDN, but high ADCC activity does not always indicate severe HDN.

Stephen F. Garner, Alan Devenish

Immunohematology, Volume 12 , ISSUE 1, 20–26

Research paper | 31-July-2017

Age‑dependent concomitant changes in synaptic function and GABAergic pathway in the APP/PS1 mouse model

of the disruption documented in the glutamate system. To build on previous research in this field, experiments were designed to determine if previously documented synaptic dysfunction in AD models is concomitantly observed with excitation/inhibition imbalance as suggested by observation of seizure and seizure‑like pathology in such models. We report changes in synaptic function in aged APPPS1 mice not observable in the younger cohort. These changes in synaptic function are furthermore accompanied

Tutu Oyelami, An De Bondt, Ilse Van den Wyngaert, Kirsten Van Hoorde, Luc Hoskens, Hamdy Shaban, John A. Kemp, Wilhelmus H. Drinkenburg

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 4, 282–293

Research paper | 01-August-2017

The false-positive responses of analgesic drugs to the intradermal serotonin- and compound 48/80-induced scratches as an animal model of itch

, 10 mg/kg), tramadol (20, 40, 80 mg/kg), cannabinoid agonist CP 55,940 (0.1, 0.3, 1 mg/kg), paracetamol (100, 200, 300 mg/kg) and diclofenac (50, 100, 200 mg/kg) were given intraperitoneally 30 min prior to pruritogen injection. The analgesic drugs dose dependently blocked serotonin and compound 48/80-induced straching behavior with exerting complete inhibition at certain doses. Our data suggests that intradermal pruritogen-induced scratching models may not discriminate pain and itch sensations

Fatih Ilkaya, Ozgur Yesilyurt, Melik Seyrek, Ozgur Gunduz, Tayfun Ide, Ahmet Akar, Ahmet Ulugol, Hasan Guzel, Ahmet Dogrul, Durmus Ucar, Caner Gunaydin

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 3, 234–243

Original Paper | 07-June-2016

Biocontrol of Gray Mold Decay in Pear by Bacillus amyloliquefaciens Strain BA3 and its Effect on Postharvest Quality Parameters

other treatments, the inhibition effect on gray mold of washed cell suspension of B. amyloliquefaciens was the best. Consequently it was utilized in subsequent experiments. Spore germination and germ tube length of Botrytis cinerea was 18.72% and 12.85 μm treated with BA3, while the control group was 62.88% and 30.44 μm. We confirmed that increase of the concentration of B. amyloliquefaciens, improved the efficacy of BA3 in controlling gray mold decay of pears. Colonization variation of BA3 in

Hui Qu, Longyu Zhao, Fengchun Zhao, Yufang Liu, Zhengyou Yang

Polish Journal of Microbiology, Volume 65 , ISSUE 2, 171–176

original-paper | 27-March-2019

Bioactive Compounds of Pseudoalteromonas sp. IBRL PD4.8 Inhibit Growth of Fouling Bacteria and Attenuate Biofilms of Vibrio alginolyticus FB3

(approx. 1 × 106 cells/ml) were prepared in 0.9% saline and swabbed evenly on the MA. The discs were then placed on the MA surface and incubated at 30°C for 24 h. Copper omadine (CuPT) (0.002 mg/disc) and 98% methanol were utilized as positive and negative controls, respectively. The resulting zones of inhibition produced around the discs were then measured in millimeter (mm). The MIC assay of extract was undertaken according to the modified microdilution method (Yu et al. 2012). The extract, in


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 21–33

original-paper | 12-May-2020

PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

were incubated for about 2 days. After 2 days of incubation, similarly, agar plugs were cut from the fungal cultures, and were placed on the opposite side of the same plate that contained the actinomycetes agar plug. The plates were incubated for further 4 to 5 days at 27°C. After that the incubation zone of inhibition was measured. In another method, the fungal test strains, including F. oxysporum (FO), R. solani (RS), and Aspergillus sp. (FN2) were streaked on SDA (Sabourad dextrose agar). The


Polish Journal of Microbiology, Volume 69 , ISSUE 2, 139–149

Research paper | 31-July-2017

Upregulation of CCL3/MIP‑1alpha regulated by MAPKs and NF‑kappaB mediates microglial inflammatory response in LPS‑induced brain injury

brain. In the observation period, LPS induced CCL3/MIP‑1alpha expression, which localized to OX‑42‑labeled microglia, leading to time‑dependent increases in the phosphorylation of P38 MAPK and ERK1/2. The expression pattern of induced CCL3/MIP‑1alpha was partly consistent with the phosphorylation of MAPKs (P38 MAPK, ERK1/2). Anti‑MIP‑1alpha attenuated microglial accumulation and the upregulation of cyclooxygenase‑2 and iNOS. The inhibition of P38 MAPK, ERK1/2, or NF‑kappaB signaling reduced the

Xiaobo Zhu, Dee Wei, Ou Chen, Zhaohua Zhang, Jiang Xue, Shanying Huang, Weiwei Zhu, Yibiao Wang

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 4, 304–317

Original Paper | 30-June-2018

Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis

spectrum of Staphylococcus spp. clinical and animal isolates. Growth inhibition was observed only in the case of 13 (including nine S. aureus, three S. xylosus and one S. epidermidis strains) out of 206 strains tested. Important advantage of the produced agent was its high thermal stability. Fifteen minutes of incubation at 90°C did not affect its antimicrobial potential. The highest efficiency of production of the agent was demonstrated in TSB medium after 24 hours at 37°C. The researches revealed


Polish Journal of Microbiology, Volume 67 , ISSUE 2, –

Report | 01-December-2019

The production, serologic evaluation, and epitope mapping of ten murine monoclonal Dombrock antibodies

embryonic kidney 293T cells expressing high levels of Doa or Dob. The MoAbs were tested serologically with untreated and enzymatically or chemically modified red blood cells (RBCs). Serologic inhibition studies were performed with synthetic peptides corresponding to Doa and Dob amino acid sequences. Pepscan epitope analysis was done on an array of immobilized tridecapeptides corresponding to the full-length polypeptide. All ten antibodies were serologically specific for Dombrock. Eight of the antibodies

Magdalena Grodecka, Kazimiera Wasniowska, Gregory Halverson, Karina Yazdanbakhsh, Marion E. Reid, Elwira Lisowska

Immunohematology, Volume 28 , ISSUE 4, 124–129

original-paper | 19-March-2021

Campylobacter fetus Induced Proinflammatory Response in Bovine Endometrial Epithelial Cells

manufacturer’s indication to recover cell and bacteria RNA. Three independent assays with three replications of each time were performed. For cytoskeleton inhibition, before the invasion assays, cell cultures were treated with cytochalasin D (3 mM) (Sigma-Aldrich, USA) for 30 min at 37°C. Then the invasion assays were made as described previously. Intracellular survival evaluation by reverse transcription qPCR. Quantitative reverse transcription PCR using random primers and a commercial kit to synthesize


Polish Journal of Microbiology, Volume 70 , ISSUE 1, 99–106

Review Paper | 10-December-2015

Antiepileptic drugs as a new therapeutic concept for the prevention of cognitive impairment and Alzheimer’s disease. Recent advances

Introduction. Excessive accumulation of amyloid-beta (A_) peptides in the brain results initially in mild cognitive impairment (MCI) and finally in Alzheimer’s disease (AD). Evidences from experimental and clinical studies show that pathological hyperexcitability of hippocampal neurons is a very early functional impairment observed in progressive memory dysfunctions. Therefore, antiepileptic drugs (AEDs) whose mechanism of action is aimed at inhibition of such neuronal hyperexcitability

Krzysztof Sendrowski, Wojciech Sobaniec

Journal of Epileptology, Volume 23 , ISSUE 2, 139–147

original-paper | 27-March-2019

In Vitro and In Vivo Activity of Zabofloxacin and Other Fluoroquinolones Against MRSA Isolates from A University Hospital in Egypt

decrease in the viable count by ≥ 3 log10 CFU/ml after 6 h of contact when tested against a fluoroquinolone-resistant isolate and complete killing when tested against a fluoroquinolone-sensitive strain with inhibition of regrowth in both cases (Fig. 1A–D and 2A–D). Similar rapid bactericidal activity was reported by Park et al. (2016), when ZAB was tested against clinical isolates of S. pneumoniae. In S. aureus, topoisomerase IV and the A subunit of the DNA gyrase are the primary targets for quinolones


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 59–69

Article | 26-October-2020

Evaluation and comparison of three human monoclonal anti-S, two human polyclonal anti-S, and one murine anti-GPB

order to determine their potential as blood group reagents and research tools. Furthermore, through inhibition experiments, we attempted to define the epitope recognized by the antibodies. Three monoclonal (MS-93; MS-94; MS-95) and two polyclonal (A1958; XI960) anti-S and a monoclonal anti-GPB (Mab 148) were tested by standard hemagglutination with RBCs of known common and rare phenotype, with S+ RBCs treated with enzymes, with dif­ferent concentrations of NaCIO, and after incubation with

Marion Reid, Gregory R. Halverson, Janet Sutherland, Malcolm Rhodes

Immunohematology, Volume 15 , ISSUE 4, 163–166

Article | 30-November-2018


phosphorylations take place with the participation of cellular kinases. The active drug form, ganciclovir triphosphate (GCV-TP), is a substrate for the viral DNA polymerase. Therefore, the antiviral effect results from the inhibition of the viral DNA synthesis in the mechanism of the competitive incorporation of the deoxyguanosine triphosphate into the DNA chain [14, 16, 19]. Ganciclovir is structurally similar to acyclovir; however, the introduction of an additional hydroxyl group has expanded the drug

Anna Majewska, Beata Młynarczyk-Bonikowska, Magdalena Malejczyk, Sławomir Majewski, Grażyna Młynarczyk

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 3, 291–299

Article | 01-July-2019


stress [22, 44, 85, 103]. The level of (p)ppGpp in cells also regulates the process of translation and DNA repair. Functioning of the stringent response is observed in both Gram-negative and Gram-positive bacteria, and its psychological effects also include the inhibition of DNA replication [4, 23, 25, 66], which slows down the division and growth of cells [10, 23]. The RelA enzyme is a ribosome-associated protein that “senses” a deficiency in amino acids by directly monitoring the translational

Julia Berdychowska, Justyna Boniecka, Grażyna B. Dąbrowska

Postępy Mikrobiologii - Advancements of Microbiology, Volume 58 , ISSUE 2, 127–142

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